Impact Factor 5.076

The Frontiers in Neuroscience journal series is the 1st most cited in Neurosciences

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Mol. Neurosci. | doi: 10.3389/fnmol.2018.00046

CBX2 Inhibits Neurite Development by Regulating Neuron-Specific Gene Expression

 Xi Gu1*, Dazhuang Su1 and Xiaodan Jiang1
  • 1Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, China

Polycomb group (PcG) proteins regulate the epigenetic status of transcription regulatory states during development. Progression from pluripotency to differentiation requires the sequential activation and repression of different PcG target genes, however, the relationship between early patterning signals, PcG expression, and the development of the Central nervous system (CNS) is still unclear. Using various models of neuronal differentiation, we provide evidence that CBX2 is a negative regulator of neuronal differentiation. Knock-down of CBX2 expression promotes neurite development, while overexpression of CBX2 inhibits neurite development. Further, we found that CBX2 is a direct target gene of miR-124. During neuronal differentiation, CBX2 was decreased while miR-124 was increased. Mechanistically, CBX2 directly interacts with the promoter region of several neuro-associated genes and regulates their expression. We found that the neuron-specific GAP-43 gene could contribute to the stimulating effect on neurite development associated with inhibition of CBX2.

Keywords: CBX2, mir-124, neuronal gene expression and regulation, neuronal development, GAP-43 Protein

Received: 24 Oct 2017; Accepted: 02 Feb 2018.

Edited by:

Daniel F. Gilbert, Friedrich-Alexander-Universität Erlangen-Nürnberg, Germany

Reviewed by:

Keijo Viiri, University of Tampere, Finland
Pierre Billuart, Institut National de la Santé et de la Recherche Médicale (INSERM), France
Holger Scholz, Charité Universitätsmedizin Berlin, Germany  

Copyright: © 2018 Gu, Su and Jiang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Xi Gu, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China, guxi124@126.com