Sucrose Consumption Alters Serotonin/Glutamate Co-localisation Within the Prefrontal Cortex and Hippocampus of Mice

The overconsumption of sugar-sweetened food and beverages underpins the current rise in obesity rates. Sugar overconsumption induces maladaptive neuroplasticity to decrease dietary control. Although serotonin and glutamate co-localisation has been implicated in reward processing, it is still unknown how chronic sucrose consumption changes this transmission in regions associated with executive control over feeding—such as the prefrontal cortex (PFC) and dentate gyrus (DG) of the hippocampus. To address this, a total of 16 C57Bl6 mice received either 5% w/v sucrose or water as a control for 12 weeks using the Drinking-In-The-Dark paradigm (n = 8 mice per group). We then examined the effects of chronic sucrose consumption on the immunological distribution of serotonin (5-HT), vesicular glutamate transporter 3 (VGLUT3) and 5-HT+/VGLUT3+ co-localised axonal varicosities. Sucrose consumption over 12 weeks decreased the number of 5-HT–/VGLUT3+ and 5-HT+/VGLUT3+ varicosities within the PFC and DG. The number of 5-HT+/VGLUT3– varicosities remained unchanged within the PFC but decreased in the DG following sucrose consumption. Given that serotonin mediates DG neurogenesis through microglial migration, the number of microglia within the DG was also assessed in both experimental groups. Sucrose consumption decreased the number of DG microglia. Although the DG and PFC are associated with executive control over rewarding activities and emotional memory formation, we did not detect a subsequent change in DG neurogenesis or anxiety-like behaviour or depressive-like behaviour. Overall, these findings suggest that the chronic consumption of sugar alters serotonergic neuroplasticity within neural circuits responsible for feeding control. Although these alterations alone were not sufficient to induce changes in neurogenesis or behaviour, it is proposed that the sucrose consumption may predispose individuals to these cognitive deficits which ultimately promote further sugar intake.


INTRODUCTION
Obesity is an expanding global health issue driven by the overconsumption of high sugar foods (Jacques et al., 2019a). Sugar is an addictive substrate, as demonstrated by a plethora of historical neurobehavioral studies. For sugar to be deemed an addictive substance, the four criteria need to be validated: bingeing, withdrawal, craving and cross-sensitisation. Sugar has been shown to elicit all hallmark signs of addicted behaviours: bingeing, cross-sensitisation (Avena and Hoebel, 2003), tolerance and cravings (Avena et al., 2005). In order to decrease the prevalence of diet-induced obesity in society, it is crucial to understand how sugar elicits an addictive response.
We have previously reported that varenicline, an FDAapproved nicotinic acetylcholine receptor (nAChR) partial agonist significantly reduced long-term 5% binge-like sucrose consumption (Shariff et al., 2016). For the first time, chronic long-term 25% sucrose consumption has been recently shown to augment weigh gain, elicit abnormal hyperlocomotion, impair cognitive function and alter neurogenesis (Beecher et al., 2021). Only continuous access to 25% sucrose has been investigated in mice, therefore, considering the effect of varenicline on 5% sucrose on reducing sugar intake, we had to examine chronic restricted (DID) 5% sucrose consumption.
The addictive nature of sugar may be due to sugar-induced cognitive neuroplasticity in both the hippocampus (Kanoski and Davidson, 2011) and prefrontal cortex (PFC) (Reichelt, 2016). These areas are focally responsible for memory and executive function, respectively. Many studies have investigated the effects of cognition and sugar, however, there is variability in the literature in terms of interspecies used, concentration of sugar, the type of sugar used and the method of administration. Most of the high sucrose diet impairing spatial and recognition memory have been conducted using a rat model (Jurdak et al., 2008;Jurdak and Kanarek, 2009). There remains relatively little data available on sugar-induced cognitive deficits in mice yet alone lower concentrations of sucrose.
The 5-HT-producing neurons are located in the dorsal (DR) or median (MR) raphe nuclei in the brainstem and project to brain regions including the dentate gyrus (DG) of the hippocampus (Gras et al., 2002;Amilhon et al., 2010) and PFC (Herzog et al., 2004;Amilhon et al., 2010;Sakae et al., 2019). Serotonin signalling has been broadly implicated in the central regulation of diet-induced obesity. Activation of central serotonin receptors excites anorexigenic neurons (Sohn et al., 2011;Roepke et al., 2012), inhibits orexigenic neurons (Heisler et al., 2006). A variety of serotonin receptor subtypes such as 5-HT 1B , 5HT 2B , 5HT 2C , 5-HT 4 , and 5-HT 6 mediate weight loss and satiety (Walsh et al., 1994;Cowen et al., 1995;Sargent et al., 1997;Heisler et al., 2002Heisler et al., , 2006Conductier et al., 2005;Jean et al., 2007;Nonogaki et al., 2007;Smith et al., 2009;Banas et al., 2011). On the other hand, knockout models of central serotonin receptors increases feeding and weight gain (Tecott et al., 1995;Bouwknecht et al., 2001;Xu et al., 2008Xu et al., , 2010. There is also evidence to suggest that serotonin selectively modulates the hedonic aspect of feeding. A selective serotonin reuptake inhibitor blunts the standard appetite-related responses to highly palatable or aversive tastants (Mathes et al., 2013). Additionally, the 5-HT system has been implicated in emotional eating in humans, polymorphisms within serotonin transporter linked polymorphic region (5-HTTLPR) are associated with stressinduced eating susceptibility (Chen et al., 2015) and obesity in childhood (Miranda et al., 2017), adolescence (Sookoian et al., 2007) and adulthood (Fuemmeler et al., 2008). Together these data demonstrate a relationship between serotonin signalling within the brain, and diet-induced obesity. However, the specific serotonergic projections that influence this behaviour remain unknown. It is therefore likely that serotonin innervation within regions of the brain associated with sugar consumption, such as the PFC and DG, are at least partially responsible for sugar consumption. Hence, we predicted that chronic sugar consumption may induce a change in the serotonin innervation at the PFC and DG, which may influence the likelihood of further consumption.
Reward seeking behaviour is proposed to be modulated through the co-transmission of 5-HT neurons and glutamate through the action of vesicular glutamate transporter 3 (VGLUT3) (Liu et al., 2014;Qi et al., 2014;Wang et al., 2019). Given the essential role of the PFC in dietary restraint (Jurdak and Kanarek, 2009;Higgs et al., 2012) and the DG in memory storage, we hypothesise that sucrose consumption may alter 5-HT/VGLUT3 innervation in these regions leading to less cognitive control over reward related behaviours, such as further sucrose consumption. Therefore, in the present study, we examined the impact of chronic sucrose consumption on serotonin innervation within the PFC and DG. We found that mice that have chronically consumed sucrose have a decreased number of 5-HT and 5-HT/VGLUT3 varicosities within the PFC and DG. The DG was also found to have a decreased number of VGLUT3 varicosities within the DG, but not in the PFC. We also found that sucrose consumption decreased the number of microglia within the DG, and increased the number of pMAPK neurons in the PFC.

Animals
Sixteen C57BL/6 male mice (4 weeks old) were trained for the 16 weeks using the Drinking-In-The-Dark (DID) model of binge-like consumption, adapted to sugar (Rhodes et al., 2005;Crabbe et al., 2009Crabbe et al., , 2011Thiele et al., 2014;Belmer et al., 2018a;Patkar et al., 2019;Beecher et al., 2021). Mice were given access to one bottle of 5% (w/v) sucrose for a 2 h period Monday to Thursday and a 4 h period on Friday with acidified-filtered water available at all other times. The sugar solution was presented in 50 ml plastic falcon tubes fitted with rubber stoppers and a 6.35 cm stainless-steel sipper tube with double ball bearings. Sugar containing tubes were weighed prior to and 2 h (Monday to Thursday) or 4 h (Friday) after presentation. Sucrose intake and weights across the study can be seen in Supplementary Figure 1. Mouse weights were measured daily to calculate the adjusted g/kg intake. All procedures were approved by The University of Queensland FIGURE 1 | Experimental design of the study. Experimental design of the anxiety-related and depression-related marble burying (MB), elevated-plus-maze (EPM), open-field (OF), and forced swimming test (FST). Animals consumed 5% sucrose in the DID paradigm for 9 weeks prior to each behavioural testing and continued to be exposed to sucrose for a total of 16 weeks. Behavioural tests were conducted 1 week apart (across 4 weeks) after 24 h of sucrose withdrawal. After 14 weeks of sucrose consumption (1 week after behavioural tests concluded), three EdU injections were administered over 2 weeks (days 0, 7, 14). Animals were assigned into two groups: sucrose-withdrawn animals and water control.

Behavioural Testing
Following 9 weeks of DID sucrose consumption, four behavioural tests were conducted to assess if the sucrose consumption model used produces emotional deficits. All behavioural tests have been performed extensively in our laboratory (Belmer et al., 2018a;Patkar et al., 2019;Beecher et al., 2021). Each test was conducted 1 week apart, over 4 weeks, see Figure 1.
For the testing of withdrawal-induced anxiety-like behaviour, experiments were carried out 24 h after the last drinking session of the week. Marble burying (MB) is used to test anxiety and obsessive-compulsive disorder. When mice are placed in the cage with marbles, they will bury marbles as a defence mechanism under conditions of stress. MB is performed in novel individual plastic cages (21 × 38 × 14 cm) containing 5 cm thick sawdust bedding. Ten glass marbles (diameter 10-12 mm) were arranged on the bedding evenly spaced in 2 rows of 5 marbles. After 20 min, the number of unburied marbles is averaged from counting by two experimenters blind to the treatments. A marble covered at least two-thirds (2/3) of its size by saw dust was considered as "buried" (Deacon, 2006;Belmer et al., 2018a;Patkar et al., 2019;Beecher et al., 2021).
Elevated-plus-maze (EPM) is another measure of anxiety based on the animal's aversion to open spaces. EPM is conducted in an apparatus consisting of four arms (35 cm × 5 cm), elevated 50 cm above the floor. The closed arms are enclosed with 40 cm high walls. The experiment went for 5 min, with initial mouse placement in the centre, facing the open arm. The number of entries and time spent in each arm was recorded using ANY-maze tracking software (Stoelting, IL, United States) (Walf and Frye, 2007;Belmer et al., 2018a;Patkar et al., 2019;Beecher et al., 2021).
Open-field (OF) test is used to measure exploratory behaviour, general activity and anxious behaviour. This test is based on the animal's aversion to bright light and open spaces. OF was performed in an open arena of 30 × 30 cm. The floor is divided into 16 equal squares (7 × 7 cm) and a central region of 10 × 10 cm is considered as the centre. Mice are initially placed in one corner, and allowed to explore freely for 10 min. The number of entries in the centre was recorded using the ANYmaze software (Bailey and Crawley, 2009;Belmer et al., 2018a;Beecher et al., 2021).
The forced swimming test (FST) is commonly used to access depressive-like behaviour and is used to test the efficacy of antidepressants. The test is based on the rodent's response to the threat of drowning. The FST is conducted in a cylindrical glass container measuring 50 cm in height and a diameter of 20 cm. The immobility time was recorded using ANY-maze tracking software (Yankelevitch-Yahav et al., 2015;Belmer et al., 2018b;Beecher et al., 2021).

Immunohistochemistry
Following all behavioural tests, a total of three injections of 5-ethynyl-2'-deoxyuridine (EdU; 50 mg/kg) were given over 2 weeks (days 0, 7, and 14) to all mice to label proliferative cells (Beecher et al., 2021). This dose has been reported to label all actively dividing precursors in the mouse subgranular zone of the DG (Mandyam et al., 2007). Twenty-four hours after the last EdU injection, mice were transcardially perfused with 4% paraformaldehyde. Brains were harvested and postfixed overnight at 4 • C in 4% paraformaldehyde and then kept in 0.1 M phosphate buffer saline (PBS) containing 0.02% (w/v) sodium azide (PBS-azide) until histology and immunohistochemistry processing. Thirty micron-thick coronal vibratome sections were collected and kept floating in ice-cold PBS-azide. The sections were permeabilised in 1% Triton X100, 0.1% Tween-20 in PBS for 1 h and then incubated in blocking solution for 1 h at room temperature (2% normal goat serum-NGS, 0.3% Triton X100 and 0.05% Tween-20).

Imaging and Analysis
Whole PFC and DG of the hippocampus from three coronal sections per animal group (n = 8) were imaged on the Olympus FV3000 confocal microscope using a 40 × oil-objective, ×1.5 numerical zoom and 0.5 z-step. Images were deconvolved using Huygens professional v16.10 (Scientific Volume Imaging, The Netherlands) with iteration number set at 100, quality threshold at 0.001, signal to noise ratio at 15 for the 4 channels and converted in. OIF for subsequent quantification for 5-HT, VGLUT3, 5-HT/VGLUT3, and pMAPK in Imaris 9.1.2 using Surface Reconstruction and Spot Detection functions as previously described (Belmer et al., 2017. Using surface rendering, fluorescence thresholding, and masking of unwanted immunolabelling, we obtained 3D objects (structures) of VGLUT3 labelling within 5-HT boutons. All images were batch processed using the same surface thresholding parameters and mean fluorescence intensities of VGLUT3 labelling within 5-HT boutons and image volumes were obtained from the surface statistics in Imaris.

Statistics
Comparisons between groups were statistically analysed using t-tests through GraphPad Prism 9 (Graph Pad Software Co., CA, United States). A p < 0.05 will be considered significant. All values are expressed as the mean ± SEM.

Sucrose Alters the Density of the Neuronal Plasticity Marker pMAPK in the PFC
The PFC is known to exert restraint over hedonic eating behaviour (Hare et al., 2009) and a decrease in PFC activity has been associated with the breakdown of healthy dieting (Demos et al., 2011). Additionally, serotonin may also induce pMAPKassociated neuroplasticity (Michael et al., 1998). Therefore, we predicted that sucrose consumption will decrease plasticity within the PFC, reducing its inhibitory control over hedonic food consumption. To evaluate this, counted the number of pMAPKimmunoreactive neurons and reconstructed pMAPK neurons using Imaris to quantify the volume of pMAPK (neuroplasticity marker) neurons/staining in the PFC (Figure 2A). No change in the number of pMAPK neurons was observed in the PFC (Figures 2B,B'; p = 0.9177, t-test). A significant reduction in the volume of pMAPK neurons/staining in the PFC between sucrose and water consuming mice was detected (Figures 2C,C'; p = 0.0434, t-test). Although we have not seen a difference in the number of pMAPK neurons we have observed a reduction in the dendritic complexity of these neurons suggesting a decrease in the plasticity of these neurons.

Sucrose Alters Serotonin Innervation in the PFC and DG
Co-release of serotonin and glutamate in the limbic system has been proposed to play a pivotal role in the development of emotional deficits like anxiety, depression and addiction. As the serotonin system is widely implicated in ethanol and sucrose consumption, and VGLUT3 expression is modulated by alcohol and sugar intake (Tukey et al., 2013;Vrettou et al., 2019), we investigated the density of varicosities that would be able to release and co-release 5-HT and glutamate in the PFC ( Figure 3A) and DG ( Figure 3E). Sucrose drinking selectively reduces the number of 5-HT varicosities in the DG (Figure 3F; * * * * p = 0.0001, t-test) not in the PFC (Figure 3B; NS, p = 0.3394, t-test). Sucrose drinking also selectively reduces the density varicosities that release VGLUT3 in PFC ( Figure 3C; * * * * p = 0.0001, t-test) and DG ( Figure 3G; * * * * p = 0.0001, t-test). Sucrose drinking selectively decreased the number of putative 5-HT/VGLUT3 co-release sites in both the PFC and DG (Figures 3D,H; D: * p = 0.0201, H: * p = 0.0238, t-test).
Here for the first time, we have shown chronic restricted sucrose consumption produces changes in the serotonergic innervation of the PFC and DG of the hippocampus.

Sucrose Does Not Alter Adult Hippocampal Neurogenesis but Reduces DG Microglia Density
It has well been established that a reduction in serotonin levels is correlated with anxiety and depression. This depression and anxiety are then partially caused by the impairment of neurogenesis which is known as the neurogenic theory of depression and anxiety (Miller and Hen, 2015). Serotonin may therefore also control sucrose intake through cognitive changes resulting from neurogenesis within the DG. Fructoseconsuming rats (van der Borght et al., 2011) and mice FIGURE 4 | Sucrose consumption alters microglia in the dentate gyrus of the hippocampus. (A) Representative images at both 4× (left images; scale bar = 100 µm) and 40× magnification (field indicated by white boxes on 4× magnification images; right images; scale bar = 10 µm). (B) Long-term sucrose consumption reduces the number of microglia (magenta) in the DG of the hippocampus. All images are co-localised with DAPI (blue). Data are presented as mean ± S.E.M (t-test); n = 8 mice/group. *p < 0.05. (Cisternas et al., 2015) show neurogenic deficits within the DG. The importance of serotonin in neurogenesis has been shown as the selective serotonin reuptake inhibitors mediate their antidepressive responses through binding 5-HT 2B receptors in the DG (Diaz et al., 2012) and subsequently altering hippocampal neurogenesis (Alenina and Klempin, 2015). Upon 5-HT 2B receptor activation, DG microglia migrate toward neuroblast cells (Kolodziejczak et al., 2015), a process essential for neurogenesis (Kreisel et al., 2019). As we have observed a reduction in density of 5-HT, VGLUT3 and 5-HT/VGLUT3 varicosities, we therefore assessed sucrose effect on emotional behaviour after 9 weeks of sucrose, and neurogenesis after 16 weeks of sucrose consumption.
We performed four behavioural tests assessing anxiety and depression: MB, EPM, OF and FST. Surprisingly, we did not observe any anxiety-like behaviour or depressive-like symptoms after sucrose withdrawal (Supplementary Figure 2). Supporting the neurogenic theory of depression, we also did not observe any neurogenic deficits across any stage of neurogenesis in the DG of the hippocampus (Supplementary Figure 3) which was interesting after observing changes in serotonin and knowing that serotonin mediates DG neurogenesis. Glial cells are key mediators throughout neurogenesis, therefore we also assessed glial cells after sucrose consumption. We did not see any changes in number of oligodendrocyte progenitor cells; however, we did observe a reduction in the number of microglia in the DG as a result of sucrose consumption (Figures 4A,B; * p = 0.0243). The dual impact of decreased 5-HT and decreased microglia was not sufficient to elicit any negative impact on neurogenesis.

DISCUSSION
This study demonstrates that chronic consumption of low concentration of sucrose leads to a decrease in the expression of the plasticity marker pMAPK and reduction in serotonin innervation within the PFC and DG, please see Figure 5 for summary of key findings. Given that these structures modulate emotions and behaviours associated with feeding (Kanoski and Davidson, 2011;Reichelt, 2016), these findings demonstrate how chronic sugar consumption can alter the central regulation of feeding from a molecular perspective. It is interesting that we do not observe any behavioural changes considering the effect of varenicline on reducing 5% sucrose intake (Shariff et al., 2016). However, small changes in serotonin co-localisation, neuroplasticity and microglia were observed. These results may predispose individuals to the delirious effects seen at higher concentrations of sucrose which ultimately promote further sugar intake.
Both high sugar and high-fat diets reduce the expression of genes involved in neuroplasticity. High-fat diets have shown to reduce hippocampal levels of brain-derived neurotrophic factor (BDNF) as well as cyclic AMP-response element-binding protein (CREB) mRNA and protein levels (Maniam et al., 2016). BDNF controls CREB activation and is important in memory formation with pMAPK being downstream of BDNF and upstream of CREB. Greater activation of prefrontal pMAPK has been observed following binge-ethanol consumption (Agoglia et al., 2015). Additionally, serotonin (Michael et al., 1998) and serotonin receptors (Yuen et al., 2008;Cui et al., 2016) also modulate pMAPK-associated neuroplasticity. Therefore, we wanted to determine the effect of sucrose consumption on pMAPK expression. In contrast to ethanol exposure (Agoglia et al., 2015), we observed a reduction in the overall immunoreactivity of pMAPK in the PFC. Changes in dendritic MAPK phosphorylation at the PFC, similar to what we have reported, has been correlated with altered apical dendritic structure (Papadeas et al., 2008). Therefore, dendritic mapping of the PFC following chronic sucrose consumption could be conducted to confirm if these structural changes are also present. In summary, these results suggest that sucrose negatively affects neuroplasticity in the PFC. Future work can investigate these regions associated with cognitive function by performing behavioural testing such as the Morris Water Maze.
FIGURE 5 | Summary of key findings. Serotonergic neurons in the RN (coloured red; dorsal, median and caudal raphe nuclei) project to the PFC and DG to modulate behaviours associated with eating. Chronic, low-concentration sucrose consumption causes a decrease in the density of pMAPK, VGLUT3 and 5-HT/VGLUT3 co-localisation in the PFC (coloured blue). In the DG of the hippocampus (coloured grey), sucrose consumption decreases the density of 5-HT, VGLUT3 and 5-HT/VGLUT3 co-localisation. RN, raphe nuclei; DG, dentate gyrus; PFC, prefrontal cortex.
The co-release of 5-HT and glutamate has been evidenced by the presence of the vesicular glutamate transporter 3 (VGLUT3) in some 5-HT varicosities and the co-release of the 2 neurotransmitters has been proposed to play an important role in controlling the reward system (Liu et al., 2014;Sengupta et al., 2017) and anxiety-related behaviours (Amilhon et al., 2010;Sakae et al., 2019). We therefore assessed the effect of sucrose consumption on the distribution of co-localised serotonin/glutamate in the PFC and DG. We observed a reduction in the density of 5-HT varicosities only in the DG, not the PFC. However, we saw a reduction in the density of VGLUT3 boutons in both DG and PFC and a reduction in the density of 5-HT/VGLUT3 varicosities. Decreased VGLUT3 expression may be associated with susceptibility to stress (Zou et al., 2020), however, the role VGLUT3 plays in sucrose consumption is unknown.
Cognitive and behavioural changes following VGLUT3 knockout/downregulation may not be regulated by serotonergic neurons. Neuronal projections from the dorsal raphe that contain VGLUT3, but not 5-HT have been previously documented (Commons, 2009;Hioki et al., 2010). These non-serotonergic neurons have also been implicated in reward (McDevitt et al., 2014) and have even been shown to mimic addictive phenotypes produced by VGLUT3 knockout. For example, the knockout of specifically cholinergic, but not serotonergic VGLUT3 neurons, is responsible for the aforementioned amphetamine-sensitisation (Mansouri-Guilani et al., 2019). It is therefore important that future studies be performed which immunologically dissect these VGLUT3 projections, for example by additionally probing for acetylcholine axons.
It is interesting that we did not see a reduction in 5-HT in the PFC yet we saw reduction in VGLUT3 and 5-HT/VGLUT3 varicosities considering the proposed mechanism of VGLUT3 is to synergise the filling of 5-HT and glutamate within synaptic vesicles (Amilhon et al., 2010). In an assessment of multiple brain regions, only the hippocampus displayed decreased serotonin levels as a result of VGLUT3 knockout (Amilhon et al., 2010). This suggests there is likely a unique mechanism within the hippocampus in which VGLUT3 modulates 5-HT uptake. Therefore, the decrease in DG 5-HT varicosities seen in sugar consuming mice may be a downstream effect of reduced VGLUT3 varicosities at the DG. As pharmacological agents that specifically target VGLUT3 are still being developed (Poirel et al., 2020), it is difficult to directly manipulate VGLUT3 activity in vivo to test this hypothesis. Alterations of the serotonergic and glutamatergic co-localisation within PFC and DG are supported by immunohistochemistry data. The co-localised serotonergic and glutamatergic alterations observed within PFC and DG need to be confirmed using functional studies. Future studies can measure the concentration of serotonin or glutamate in vivo using microdialysis studies. Functional experiments such as ex vivo electrophysiological studies will validate whether serotonin and glutamate are released from the same axons.
We also report that the DG microglial population is decreased following sucrose consumption. Microglia phagocytose apoptotic neuronal precursors to promote hippocampal neurogenesis under physiological conditions (Sierra et al., 2010) through the secretion of a plethora of neurogenic factors (Battista et al., 2006;Butovsky et al., 2006). Microglial signalling in the adult DG has also been shown to alter addictive behaviour (Rivera et al., 2019). Our results show that sucrose consumption influences neurogenesis through two possible mechanisms: a decrease in the number of microglia, as well as a decrease in 5-HT. Given that 5-HT promotes microglial migration in the DG during neurogenesis (Kolodziejczak et al., 2015;Kreisel et al., 2019), we expected to detect a resulting decrease in neurogenesis. Similar rodent studies using a high-fat diet have recorded decreased neurogenesis in mice (Yoo et al., 2014) and unaltered neurogenesis in rats (Rivera et al., 2013).
Our results show that, despite decreases in 5-HT and microglia in the DG, neurogenesis was unaltered in sucrose consuming mice. Therefore, these mice may not have been exposed to sucrose for a length of time where the decreased serotonergic innervation in the DG to alter neurogenesis, given that to higher concentrations of sucrose for 12 weeks elicits neurogenic deficits (Beecher et al., 2021). For example, after 10 days of SSRI administration, the DG of Sprague-Dawley rats did not display altered neurogenesis, but did show increased neuroplasticity (Pawluski et al., 2020), suggesting the onset of plasticity in this region precedes alterations in neurogenesis. We therefore hypothesised that our mice were not consuming sucrose long enough for the altered DG serotonergic input to impact neurogenesis. This potential delay in neurogenesis also suggests that higher sucrose concentrations may increase the speed of neuroplastic events, given that similar studies with similar timeframes of consumption have used higher sucrose concentrations (Kim et al., 2018;Xu and Reichelt, 2018;Beecher et al., 2021). However, sucrose consumption may not decrease the overall amount of neurogenesis but may impact the quality of the generated circuits. For example, transient microglial ablation within the olfactory bulb (another site for adult neurogenesis), results in the same number of adult neurons (granular cells) being produced, however their synaptic connectivity and odour detection is dampened (Wallace et al., 2020). This may explain why no changes in neurogenesis were seen in this study despite decreased microglial presence and 5-HT innervation within the DG. A trend does exist suggesting that sucrose consumption may increase microgliogenesis. This trend may represent a compensatory mechanism to restore the depleted microglial population seen after sucrose consumption and could potentially contribute to the lack of behavioural change.
Surprisingly, no changes in behaviour were identified across the four tests conducted. This indicates that the changes in glutamate/serotonin co-release sites detected within the PFC, as well as decreased microglial cell count and 5-HT varicosities in the DG were not sufficient to produce emotional or cognitive deficits. Once again, this may be due to inadequate exposure to sucrose, given the delay needed to impact neurogenesis (Pawluski et al., 2020), as well as the relatively low concentration of sucrose used.
This study demonstrates the potency of chronic, lowconcentration sucrose consumption in producing intricate alterations in serotonergic neuroplasticity within key brain regions associated with feeding. Although the findings of this study suggest that sucrose consumption does not alter adult hippocampal neurogenesis, we have previously shown that sucrose alters neurogenesis at higher concentrations of sucrose (Beecher et al., 2021). Therefore, it is suggested that higher sucrose concentrations, and increased exposure to the sucrose solution is required to detect more specific changes in neurogenesis. Additionally, while proliferation in the DG was assessed in response to a high-sucrose diet, cell death was not studied. High-fat diets in rodents cause increased DG apoptosis in rats (Maniam et al., 2016) as well as decreased DG neuroprogenitor cell survival in mice (Yoo et al., 2014). Therefore, DG cell death should be assessed in sucrose consuming mice through TUNEL assays in order to fully understand sucrose's effect on neurogenesis. Lastly, the current study is limited to only male C57BL/6 mice. Differences in diet-responsive neurogenic defects exist between species (Rivera et al., 2013;Yoo et al., 2014) and sex-specific differences in serotonergic function have been documented within the rat PFC (Brivio et al., 2018). Strain-specific differences in VGLUT3 expression and knockout phenotype also exist (Sakae et al., 2019). Care should therefore be taken when extrapolating these results into contexts of female rodents, or other mouse strains. Despite these limitations, this study provides novel insights into the mechanisms of sugar-induced neuroplasticity, with potential implications for obesity and eating disorder research.

DATA AVAILABILITY STATEMENT
The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author/s.

AUTHOR CONTRIBUTIONS
KB, AB, and SEB were responsible for study concept and design. KB, JW, and AJ carried out drinking experiments and behavioral experiments. KB, AJ, and NC performed immunohistochemistry and imaging. KB, JW, and FC analyzed the data, interpreted the findings, and drafted the manuscript and figures. FC, AB, and SEB edited the manuscript.

FUNDING
This study was supported by funding by the NHMRC (GNT1146417).

ACKNOWLEDGMENTS
We acknowledge the National Health and Medical Research Council and the Australian Research Council for funding of this work. We are grateful to the imaging facility of the Translational Research Institute, the facility manager Sandrine Roy and the microscopy officer Ali Ju.