Integrating the latest biological advances in the key steps of a food packaging life cycle

This literature review provides a focus on the potential of integrating the latest scientific and technological advances in the biological field to improve the status of the key steps of a food packaging life cycle: production, usage, post-usage, and long-term fate. A case study of such multi-biological food packaging is demonstrated based on the use of PHAs (polyhydroxyalkanoates) polymer, a microbiologically produced polymer from non-food renewable resources, activated by the use of bioactive components to enhance its usage benefits by reducing food loss and waste, displaying potential for reusability, compostability as post-usage, and finally, being ultimately biodegradable in most common natural conditions to considerably reduce the negative impact that persistent plastics have on the environment. We discuss how designing safe and efficient multi “bio” food packaging implies finding a compromise between sometimes contradictory functional properties. For example, active antimicrobials help preserve food but can hamper the ultimate biodegradation rate of the polymer. This review presents such antagonisms as well as techniques (e.g., coatings, nanoencapsulation) and tools (e.g., release kinetic) that can help design optimized, safe, and efficient active food packaging.


Introduction
Although packaging plays a crucial role in the food supply chain by maintaining the quality and safety of food for a certain period, environmental problems due to resource depletion and persistent plastic accumulation from food packaging are of increasing concern. Indeed, the world's plastic production is expected to increase three-fold by 2060, and ⅓ of this production will be used for packaging development (1,2). In the meantime, 90% of this plastic ends up in the environment (through littering, landfilling, mismanagement, etc.): 3% directly in the ocean and 87% on the terrestrial continent (in soil), where they are fragmented through physical abrasion into micro and nanoparticles that diffuse into all environmental compartments (air, soil, water, and living organisms), resulting in considerable environmental and biological damage (1,3). In this context, the emergence of new materials allowing the overall packaging sustainability to be increased is paramount: it clearly means (1) minimizing overall resource use and process step impacts, (2) ensuring product preservation to reduce food loss and waste, (3) reducing the burden of post-usage plastic waste management (e.g., by being compostable or recyclable) and, (4) paying special attention to the long-term outcome that can lead to the accumulation of plastic in the environment in the final stage of the materials life chain (3)(4)(5). One solution to address these multiple issues is to focus on materials that provide a biological solution at each stage: a biological resource, bioprocess transformation, bioactivity to better preserve food, and finally biological digestibility in the post-usage stages ( Figure 1). Therefore, to correctly use biobased resources for packaging development (e.g., plant or microbial polymers), it is necessary to use materials that are compatible with biomass temporal availability and the regeneration time and to avoid competition with food and feed uses. Moreover, bioprocess transformation refers to the use of biotechnologies to synthesize the material (e.g., microbial cells) with the net advantage that the range of resources to be used is considerably enlarged. For instance, with biotechnologies, it is possible to use organic wastes and other residues (6,7). Bioprocesses also appear to be good alternatives to conventional chemical synthesis, because they require less time and additives, which can result in health problems in living marine and terrestrial organisms (endocrine disruptors, etc.) (8)(9)(10). Bioactive packaging refers to the integration of active bio components in the polymer, such as natural antimicrobials or antioxidants which can increase the packaging usage benefit by preserving food from deterioration, which leads to food loss and waste (FLW) mitigation, as demonstrated by several authors (11)(12)(13). Finally, biodegradable packaging means that the material could be disintegrated and then assimilated by microorganisms and digested into small unitary molecules (H 2 O, CO 2 , CH 4 ) to produce a new microbial biomass. Ultimate biodegradation is currently the only solution to reduce conventional plastic accumulation in the environment (3).
Although several articles have already highlighted the importance of biodegradable packaging [see among others (14)], or the relevance of active, and smart biodegradable packaging for the food sector (15), or the applicability of biobased packaging for long-shelf-life foods (16), no publications to date have addressed all the four 'bio' aspects in a holistic approach to the packaging sustainability. It should be noted that in the web of sciences with the keywords "biodegradable, bioactive packaging" AND "biodegradable, bioactive, biobased packaging" AND "biodegradable, antimicrobial packaging" AND "biodegradable, biobased, antimicrobial packaging, " we obtained 90,5,215, and 6 review articles, respectively (carried out in February 2023).
In this context, the aim of this paper is to discuss the relevance of developing active packaging materials combining the four "bio" aspects (biobased, bioprocessed, bioactive, and biodegradable) by highlighting their challenges and limitations. Examples will be provided from commercial products or polymers that are still in development (e.g., PHAs, a family of polymers that, promisingly, covers the four "bio" aspects). This review highlights the importance of a reasoned-integrated approach to develop bio-benign, biodegradable, efficient active food packaging starting from the food needs in terms of preservation without compromising the ultimate biodegradability of the material. The importance of the use of biobased, bioprocessed, and biodegradable material will be discussed first, with examples of different polymers more or less in accordance with these aspects. The importance of the strategy of bioactive molecule incorporation is then developed, with the presentation of cutting-edge ways for incorporating active ingredients into biopolymers. Finally, the post-usage fate of the bioactive, biodegradable packaging materials is discussed.
2. The importance of using biobased, bioprocessed, and biodegradable materials for food packaging 2.1. Definition of biobased, bioprocessed, and biodegradable materials 2.1.1. Biobased The fear of a near future where oil-based resources are lacking and the societal demand for more 'green' materials have led to the FIGURE 1 The expected effect of substituting fossil-based plastic materials with BIO 4 ones -in the life cycle of food packaging materials (BIO 4 = biobased, bioprocessed, bioactive, and biodegradable).
Frontiers in Nutrition 03 frontiersin.org development of polymers derived from renewable biological resources ('biobased'). For this purpose, research efforts have long focused on transforming plant or other non-fossil biogenic feedstocks into materials identical (e.g., bio-PET, bio-PE) or close to those derived from petroleum (e.g., PLA). At the same time, efforts have been made regarding the development of biodegradable solutions to solve the issue of plastic persistence in the environment, leading to the emergence, in the early 1990s, of the 'bioplastics' market, which includes both biodegradable and biobased plastics, with unfortunately a high degree of confusion regarding the distinction between them (17,18). "Biobased" means materials partially or fully produced from biomass resources (such as sugar cane, hydrolyzed maize, rice or potato starch, etc.), irrespective of the end-of-life fate of the material (17). As such, a biobased polymer may be not biodegradable, such as bio-polyethylene (bio-PE), which is not more biodegradable than its oil-based counterpart. Several publications have sought to clarify this terminology and denounce the variations coming from the overall misinterpretation of the 'bio' prefix (17)(18)(19)).

Bioprocessed
Two categories of biobased polymers can be considered (20): the first one consists of materials whereby the chemical structure of the biomass feedstock is not maintained. This is the case, for instance, when biomass is used to obtain soluble sugars, which are then fermented to produce monomers for the polymerization (e.g., lactic acid to produce poly(lactic acid) or ethylene to produce biobased polyethylene). This is the route followed to obtain polymers that are identical to those derived from petroleum (e.g., bio-PET, bio-PE). The second category corresponds to a group of polymers directly synthetized by living organisms (plants, algae, microorganisms, etc.) for which the initial chemical structure is preserved after extraction and purification and then used as such [e.g., polyhydroxyalkanoates (21)], polymalic acid (22) or only slightly modified [e.g., celluloses acetates (23)].
To manufacture compounds belonging to the first category, significant efforts must be placed on the plant transformation, with selective chemical processes required to remove chemical functionality afforded naturally by many biobased feedstocks (e.g., many natural molecules contain oxygen or nitrogen, while this is not the case for fossil-based hydrocarbons). Agricultural crops produce precursors: mostly starch and soluble sugars, that can be directly used to obtain biobased polymers, or building blocks derived from selective transformation used to extract, fractionate, and deconstruct plant cellwall polymers (cellulose, hemicellulose, pectin, lignin, etc.) (24). Additional chemical transformations are often needed to obtain the necessary functionality for polymerization of the biobased building blocks into polymers.
Beyond the economic and environmental cost of all this necessary chemistry, the use of dedicated agricultural crops for producing biobased polymers is highly controversial in a period of fears for worldwide food security (25). Most lifecycle analyses (LCA) show that biobased polymers are better than their oil-based equivalents in aspects such as GHG emissions and fossil fuel consumption but not for other indicators such as eutrophication, land occupation, etc., which leaves the question of environmental superiority of biobased polymers for further debate (26,27).
In light of the questionable economic viability and environmental issues of biobased polymers chemically synthesized from biobased building blocks, a reasonable guideline would be to focus on the second category of polymers, those directly synthetized by living organisms and used after extraction/purification with minimal chemical transformations. To not compete with the food chain, they should be produced from agricultural or food by-products or residues. The use of residues and even organic wastes as feedstock to produce polymers is gaining more and more attention, especially in line with the development of circular economy schemes (7). They provide significant environmental benefit, provided that their collection, transport, and transformation are regionally-reasoned and do not necessitate expensive pre-treatments and transportation (28, 29).

Biodegradable
Biodegradability is the intrinsic property of a material to be fully degraded by living microorganisms (e.g., soil microorganisms) into a new biomass and small non-toxic molecules such as water, carbon dioxide (CO 2 ), and/or methane (CH 4 ) in widespread natural environmental conditions and in a reasonable timeframe compatible with human life cycles (17). The term biodegradable is often and misleadingly applied to plastics that are biobased but not necessarily biodegradable, or that undergo degradation (e.g., lowering of the molar masses of macromolecules that form the material) but without complete, ultimate mineralization (30). The biodegradability of a polymer depends only on its chemical structure and not the carbon source. Polymers that are compostable are not necessarily biodegradable under ambient environmental conditions. For instance, PLA needs to reach a minimal temperature of approximately 60°C to be biodegraded, which can be achieved in industrial composting plants but not in soil for instance or even domestic compost (19). The risk of considering a polymer that turns out not to be biodegradable under ambient environmental conditions is the production of persistent micro and nano plastics with long-term adverse effects for the entire biosphere (3).
In light of the above, nonfood, nonfeed biobased, and minimally bioprocessed polymers appear to be the best compromise of biobased and bioprocessed polymers. In addition, care must be taken regarding the type of biodegradation that the material can achieve: ultimate biodegradation, e.g., complete degradation into mineral molecules, under common ambient environmental conditions, and in a reasonable timeframe are paramount.  Frontiers in Nutrition 05 frontiersin.org in development. Clearly, currently marketed biopolymers are not fully "bio" from their resources nor their end-of-life, or present limited usage benefit. They are either derived from food resources (e.g., PLA, PBSA, starch-based blends, or commercial PHBV) or not fully biodegradable under natural conditions (e.g., PLA), or not water resistant (e.g., starch-based blends).
Only cellulose-based materials and PHAs obtained from non-food resources (such as PHBV) appear to fulfill all the criteria.

PHAs, an example of biobased, bioprocessed, and biodegradable polymers
PHAs comprise a family of polymers with very versatile properties (water-insoluble, hydrophobic, thermoplastic). They can be molded into rigid or semi-rigid items using thermomechanical processes, and in their final shape, they exhibit good oxygen barrier properties that make them promising for fresh and other processed food packaging applications (31).
Commercial PHAs are currently nearly exclusively the copolymer polyhydroxy(butyrate-co-valerate), P(HB-co-HV). P(HB-co-HV) is a copolymer, meaning that it is a combination of two monomers, namely hydroxy-butyrate and hydroxy-valerate. P(HB-co-HV) is currently produced using pure microbial cultures fed with high-purity substrates that require (1) sterility (2) high energy, and (3) pure glucose or corn steep liquor as feedstock (48% of the total production cost). This contributes to a prohibitive market price (5 €/kg) to be used as a commodity polymer.
To address this issue, and to create a virtuous cycle rather than depleting one by using organic residues instead of apure carbon source, researchers have developed bioconversion processes of agrifood residues using optimized eco-efficient mixed microbial cultures (MMC). These processes decrease the investments and operating costs of the P(HB-co-HV) conversion with respect to pure culture and make it easier to use cheaper by-products such as feedstock (6,32,33). However, these processes are not yet available at an industrial scale.
Once produced, PHA macromolecules must be extracted and purified to remove all impurities (e.g., proteins, inorganic compounds) that can hamper its further use as a thermomechanical processable polymer. To do this, after harvesting cells from the bioreactor, flocculation, centrifugation, or filtration is used to remove the aqueous phase from the sedimented biomass. After a thermal drying or lyophilization step, the PHA-rich biomass is ready for extraction and purification (34,35). Most people utilize methods on a laboratory scale such as solvent-based extraction (using halogenated solvents or halogen-free solvents such as butanol, or acetone, etc.), enzymatic (Alcalase ® , lysozyme) or mechanical techniques (ultrasonication, high-pressure homogenization, osmotic pressure), or combinations of these methods. These methods result in cell disruption, enabling polymer solubilization in the solvent used. The last steps are then washing and drying the polymer to obtain a powder that can be stored and subsequently used (36).
PHA granules are often damaged by random and chain-end scission during solvent-based extraction processes. The final PHA purity and quality (e.g., molecular weight and polydispersity index) are thus affected by the extraction method. The solvent extraction method (especially halogenated solvents) provides the highest recovery with the highest purity and molecular weight (up to 98% and 0.8 MDa, respectively) (33,(35)(36)(37)(38). However, because of the cost and the environmental impact of solvents, their use remains possible only at the laboratory scale, for research purposes. Beyond extraction, purification is also very important and has been found to greatly impact the processability of the polymer and its final properties (39); it was observed that multiple purification steps lead to better P(HB-co-HV) mechanical properties after processing. Because considerable quantities of hazardous solvents and energy are necessary during the extraction and purification steps, PHA production is not fully bio-benign. Moreover, the halogenated solvents used for the extraction can cause severe health issues in living organisms as well as environmental problems (40).
Above all, PHAs are biodegradable under various composting conditions (including home-compositing) and other natural environments (e.g., soil), making them particularly suitable to fight pollution by persistent plastics. As a result of successful tests on the biodegradation of PHA in activated sludge, compost, aqueous environments, and soil, the biodegradation of PHA blends with natural fillers in composting conditions is faster than with pure PHAs (41)(42)(43). This high biodegradation rate is the result of the involvement of a wide range of microorganisms and fungi, similar to the ones used for PHA biosynthesis, that can biodegrade PHAs in anaerobic and aerobic conditions (37,(44)(45)(46)(47)(48). Environmental factors such as temperature, pH, moisture, oxygen concentration, sunlight, the number/amount of microorganisms, as well as the chemical structure of PHA such as the percentage of 3 HV, are known to affect the rate of biodegradation of these polymers (49).
In summary, PHAs can be synthesized from agri-food wastes and residues using bioprocessed (microbial synthesis) and they are biodegradable under natural conditions and ambient temperature. They are thus promising candidates to turn the current linear plastic chain into a more sustainable cycle. Moreover, PHAs exhibit good usage benefits, with high gas/water barrier properties as well as good crystallization and mechanical properties; making them good candidates to prolong the shelf-life of food products. PHAs can also be converted into bioactive materials to increase their packaging usage benefit of reducing food losses.
3. The importance of bioactive functionality in food packaging 3.1. Bioactive packaging to better preserve food from deterioration Commission Regulation [EC 450/2009 (50)] defined Active Packaging (AP) materials as follows "Materials and articles that are intended to extend the shelf-life, or to maintain or improve the condition, of packaged food; they are designed to deliberately incorporate components that would release or absorb substances into or from the packaged food or the environment surrounding the food." As described in Table 2, various scavenging and releasing systems have been developed, aimed at decreasing the It should be noted that essential oils extracted from natural aromatic plants (e.g., oregano, thyme, eucalyptus, rosemary, clove, cinnamon etc.) is often used as natural antioxidant, antimicrobial, and antifungal compounds for AP (68-70). Indeed, consumer demand for food products without synthetic chemical preservatives has promoted EO usage in the food packaging industry in recent years (68). As for all AP, the use of active components directly in the packaging material instead of in the food allow reduction of the use of preservatives, as the active component is slowly released toward the surface where it is needed (on the food surface).
To obtain safe and efficient active materials, the quantity of active compounds added in the formulation of active systems is defined by the expected efficiency but also regulatory needs and sensory aspects (e.g., active compounds that are also flavoring agents). Indeed, a specific admissible daily intake (ADI = the quantity of this specific compound that can be eaten without a negative impact on the health) is defined by regulations for all potential active substances (71). The use of the active molecules is possible only if the natural exposure of consumers to those substances from other (natural) sources (natural occurrence in food products due to their composition) does not exceed the ADI (Figure 2). The natural exposure depends on the country, food consumption habits, and the consumer's age. This knowledge allows determination of the maximal quantity of the AC that can be used for active packaging (ADI minus the natural exposure) (Figure 2).
The choice of the best molecule to develop an active antimicrobial packaging also depends on the capacity of the molecules to act on the targeting of specific microorganisms. Consequently, the appropriate AC can differ from one product to another, depending on the microorganisms prone to growth on the product. To correctly design the active packaging, it is necessary to confirm that the quantity that can be used for the active packaging, while remaining below the difference between the ADI and natural exposure, is enough to reach the minimal inhibitory concentration (MIC) of the targeted microorganisms [EC 450/2009 (50)] (Figure 2). The use of modeling tools, taking into account the release and migration (for volatile molecules) or diffusion characteristics (for non-volatile molecules) of the AC in defined storage conditions (temperature, duration) of the product, as well as MIC for the targeted microorganisms allows precise determination of the quantities of antimicrobial agents to use in packaging to preserve the food product from deterioration (13, 72). For some molecules with high odor or taste, such as essential oils, the quantity of AC to use should be below the odor threshold and avoid organoleptic modifications (Figure 2

Strategies for active functionality incorporation
The impact of antimicrobial compounds, such as essential oil, on microorganism inhibition in food is dependent on the release and migration capacity of the molecules, and as a consequence on (1) the strategy of AC incorporation in the polymer material, (2) the volatility of the AC, and (3) the interactions and bonds between the component and the polymer material (74,75). Indeed, different authors have shown that a film containing a homogenous concentration of AC throughout generated a slower and more sustained release than a film with an active coating concentrating the entire quantity of AC on the film surface ( Figure 3) (76)(77)(78). This structure of AP and strategy of AC incorporation impact the preservative action of the AC on the food (Figure 4). For example, Wicochea-Rodríguez et al. (79) showed a four-fold lower release constant for carvacrol included in soy protein isolate film compared with the equivalent solution (carvacrol in soy protein isolate) coated on paper. Moreover, the authors also showed that the production of the same films with eugenol instead of carvacrol considerably increased the release of the molecule (release rate constant 100 times higher) due to the higher volatility of the molecule and lower retention by polymer, thereby inducing a different mechanism of release.
The choice to integrate AC throughout versus on the surface of polymers can also generate different degrees of loss of the molecules, depending on the production processing. Indeed, integration of AC directly in the polymer usually implies that the AC is subjected to thermal processes, such as extrusion or compression molding, meaning that highly sensitive molecules can be degraded, thus reducing their antimicrobial activity. By contrast, integration of AC on the surface of the polymer usually implies no thermal coating process, such as a bar, knife, spray coaters, or electrospinning, which preserves AC and its antimicrobial activity. The main principle of both of these physical incorporation methods is that the active components are physically contained or dispersed within the packaging material. These methods do not involve any chemical reactions or modifications of the active components, unlike sol-gel processes, surface immobilization, cross-linking, and covalent binding methods that involve chemical reactions (covalent and non-covalent bonds) between antimicrobial molecules and the film surface (77,78,80). The advantages and disadvantages of these incorporation methods are listed in Table 3.
To overcome the issue of thermal degradation during thermomechanical shaping processes, attention has been given in the past two decades to different encapsulation strategies. Beyond mitigation and protection of AC from thermal degradation during packaging production, encapsulation may also help to control the release of the active molecules, especially if they are volatiles, and it is also used for that purpose (86). The term "encapsulation" was defined by Becerril et al. (87) and Stoleru and Brebu (88) as the technique that permits a substance (the active agent) to be encapsulated within another, resulting in tiny particles, the contents of which are gradually released under certain conditions. Hence, encapsulation strategies permit savings in regard to some active components as it is no longer necessary to compensate for the AC thermal degradation by spiking the initial load of AC in the material. In the same way, it allows minimization of the negative impact of the EOs on odors and organoleptic characteristics of the food products (89-91). Finally, encapsulation allows AC losses during storage (e.g., AC trapped into a polymer capsule) to be avoided and to trigger the release only when it is necessary, e.g., in food contact by action of humidity for instance as is the case for cyclodextrin-based capsules (92). Various carriers have been tested to develop encapsulation strategies, such as cyclodextrins, (nano)clays, nanofibers, and nanoparticles, all of which exhibit some advantages and disadvantages, as listed in Table 4 (87, 94).

How to reach the right balance of antimicrobial properties during the usage stage and biodegradation in the post-usage stage
To design bioactive and biodegradable packaging, it is crucial to consider the impact of natural antimicrobial components in polymers Principle of antimicrobial compound dimensioning.
Frontiers in Nutrition 08 frontiersin.org on the degradation capabilities of the entire packaging. Thus, it is necessary to achieve the right balance between the antimicrobial characteristics of packaging materials to increase the food shelf-life during the usage phase and the biodegradation of packaging during the post-usage phase. The influences of the incorporated bioactive components on the biodegradation properties of active biobased materials are reviewed in Table 5.
All studies (Table 5) showed that the impact of active components on polymer biodegradation was due to either (1) the antimicrobial activities of the AC or (2) modification of polymer properties owing to the presence of AC (132, 133).
In the first case, essential oils and natural bioactive components have a high probability to hamper the biodegradation of biopolymers, as they are per se antimicrobial compounds and poorly biodegradable due to the The various film structures and active volatile compound incorporation strategy (Active Compound Throughout and Active Compound in a surface coating) in packaging. Hypothetical dependence of the active functionality incorporation methods, meaning (1) in coated and (2) "Throughout" material on the migration of AC and biodegradation of the film.
Frontiers in Nutrition 09 frontiersin.org presence of quaternary carbon atoms and fused or bridged ring systems (134). Most of these AC are extracted from thyme, mint, sage, parsley seed, and spearmint oil, and they are toxic to microorganisms, including those involved in biodegradation (135,136). Various effects can be expected: a delay in the biodegradation kinetics (time lag) without further impact on the biodegradation rate, a slowing down of the  Frontiers in Nutrition 10 frontiersin.org biodegradation rate, or a decrease of the maximal biodegradation level, as shown in Figure 5. A combination of these three different impacts may also occur. An impact on the disintegration (the first step of the biodegradation process before assimilation by the microorganisms) has also been reported (108). For instance, Tampau et al. (107), showed that biodegradation of a starch/PCL/starch multilayer containing carvacrol (15 wt.% PCL) in compost remained incomplete, with a maximum of 85%, after 45 days compared to the starch/PCL/starch film without carvacrol that is 100% biodegraded in the same conditions and duration, while Piñeros-Hernandez et al. (104) showed that biodegradation of starch film containing between 5 and 20% rosemary extract (polyphenol) was retarded in compost conditions (Table 5) but that the final biodegradation level was not affected.
However, although a decrease in biodegradation because of antimicrobial activity of AC was often observed, the majority of studies cover only active packaging with a non-encapsulation AC throughout the entity (Table 5). Consequently, the impact of the incorporation strategies, i.e., encapsulation or not, coating vs. incorporation throughout, etc. is very absent from the literature. Indeed, the presence of the AC on the surface of the packaging instead of throughout the entity increases the release of the molecules during the usage stage (see Section 3.2). Therefore, biodegradation should be impacted less, as the concentration of AC is lower and the polymer is more available for microorganisms at the post-usage stage (Figure 3). In this vein, Mustapha et al. (64), showed that a decrease in the coating thickness of starch with turmeric oil induced an increase in biodegradation. Consequently, a high level of importance should be placed on the design of active packaging to find the best formulation allowing the shelf-life to be increased without delaying the Different biodegradation scenarios for active biopolymers.    biodegradation of the material. For this, mathematical modeling could be developed, coupling the alteration of the microorganism's growth in food and their impact on the product shelf-life, as well as the growth of the biodegradation microorganism and the impact on the end-of-life of the material.
In the second case, the addition of AC in polymers can either reduce or increase the polymer biodegradation due to modification of their properties. A decrease in biodegradation can be due to an increase in the molecular weight or hydrophobicity, or a decrease in flexibility, as has been observed with the presence of cinnamaldehyde (1.5 wt.% to 5 wt.%) in gliadin film during compost (102) or cinnamon (0.5 wt.%) in chitosan/starch film during biodegradation in soil (117). Indeed, EOs formed bonds with the polymer chains, and this cross-linking increased the glass transition temperature. It also restricted the entrance of water into the polymer matrix, which led to less swelling and lower water vapor diffusion coefficients. By contrast, the increase in biodegradation can be due to an increase in hydrophilicity, thermal stability, and chain mobility and a decrease in the molecular weight and crystallinity, as has been observed with the presence of green tea extract (from 10 to 30%) in PCL/PLA film in compost (118), or olive pomace (15%) in PHBV film during biodegradation in soil (137). It should be noted that AC encapsulated in nano clays, such as thymol in PLA-based nano-composites, cinnamaldehyde in PLA/organoclay, or curry/thymol in nanofibrillated cellulose/PLA, led to an increase in the film's biodegradation owing to higher sensitivity to hydrolysis of the polymeric chains, which highlights the relevance of encapsulation of the AC (65,125,128). However, the impact of AC on the biodegradation of the polymer can differ due to the amount of the AC and reach a breaking point with an increase/or decrease of the AC quantity, whereby polymer biodegradation can decrease because of significant deterioration of the polymer properties. For example, the presence of 20 wt.% green tea extract in PCL/PLA accelerated the biodegradation of the entire material owing to increased hydrophilicity, but 30 wt.% green tea extract in PCL/PLA decreased the biodegradation of the entire material owing to an increase in crystallinity (118). By contrast, the integration of 3 wt.% lignin into a cellulose nanocrystals/PLA system resulted in acceleration of the disintegration of the film, but the presence of only 1 wt.% lignin in the system resulted in slower biodegradation due to a higher degree of crystallization of the material (121).
Finally, in some rare cases, the presence of AC in the polymer did not impact the biodegradation of the entire material, as observed with lemongrass EO and lauric acid in starch (129,130), and ethyl lauroyl arginate (LAE) in a PLA/PLA-LAE/PLA-CNC/Chi structure (131).
Beyond AC incorporation that results in modification of the polymer properties, conditions met during biodegradation also affect the biodegradation rate (humidity, nature of the soil, etc.). Therefore, the type of active molecules, their incorporation strategy, their interaction with polymer, and on polymer properties, are among the many parameters that can affect (positively or negatively) the biodegradation of the bioactive material and should hence be taken into account. Beyond these observations, the comprehension of the interactions of these different parameters still needs to be deepened to better design active packaging, allowing for an increase in food shelf-life by maintaining or even enhancing the biodegradation of the packaging.

Conclusion
The continuous increase in plastic accumulation and production worldwide, and the very substantial environmental impact of food loss and waste, are the main reasons for improving the overall sustainability of food packaging materials. Therefore, an appropriate choice of material is paramount to tailoring sustainable food packaging. The use of materials derived from biobased sources, which do not compete with food and feed resources, has resulted in bioprocessing for environmentally friendly production. They are fully biodegradable by ensuring their digestibility under natural conditions, as is the case for PHAs, and they are showing great promise to enhance sustainability. Furthermore, bioactive materials help preserve food by minimizing deterioration, such as microbial development or oxidation, consequently reducing food loss and waste and its negative environmental impact. However, to design efficient antimicrobial packaging, the AC has to be present in sufficient quantity to reach the minimal inhibitory concentration of target microorganisms without surpassing the admissible daily intake. To achieve this goal, various strategies for the incorporation of AC in film (coating or throughout the polymer) have been developed with different encapsulation options, thereby enabling control of the release of the AC. However, it is crucial to achieve a proper equilibrium between antimicrobial functions during usage and biodegradability in the post-usage phase. Yet, in the case of natural active components (EOs, plant extracts, etc.), it has been shown that the amount and type of added AC, its incorporation strategy into the polymers, and its impact on the polymer's properties can either positively or negatively impact the biodegradation properties of the biopolymer. Consequently, in the future, better comprehension and prediction of the impact of AC on the shelf-life of products and biodegradation of the material should be considered as a whole, through the use of modeling tools, to better design sustainable packaging.

Author contributions
AR and FC wrote the first draft of the manuscript. All authors contributed to the conceptualization, design of the present review, manuscript revision, read, and approved the submitted version. Publisher's note All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.