Simple fabrication of electrochemical sensor based on integration of dual signal amplification by the supporting electrode and modified nanochannel array for direct and sensitive detection of vitamin B2

Development of simple and reliable sensor for detecting vitamin content is of great significance for guiding human nutrition metabolism, overseeing the quality of food or drugs, and advancing the treatment of related diseases. In this work, a simple electrochemical sensor was conveniently fabricated by modification a carbon electrode with vertically-ordered mesoporous silica film (VMSF), enabling highly sensitive electrochemical detection of vitamin B2 (VB2) based on the dual enrichment of the analyte by the supporting electrode and nanochannels. The widely used glassy carbon electrode (GCE), was preactivated using simple electrochemical polarization, The resulting preactivated GCE (p-GCE) exhibited improved potential resolution ability and enhanced peak current of VB2. Stable modification of VMSF on p-GCE (VMSF/p-GCE) was achieved without introducing another binding layer. The dual enrichment effect of the supporting p-GCE and nanochannels facilitated sensitive detection of VB2, with a linear concentration ranged from 20 nM to 7 μM and from 7 μM to 20 μM. The limit of detection (LOD), determined based on a signal-to-noise ratio of three (S/N = 3), was found to be 11 nM. The modification of ultra-small nanochannels of VMSF endowed VMSF/p-GCE with excellent anti-interference and anti-fouling performance, along with high stability. The constructed sensor demonstrated the capability to achieve direct electrochemical detection of VB2 in turbid samples including milk and leachate of compound vitamin B tablet without the need for complex sample pretreatment. The fabricated electrochemical can be easily regenerated and has high reusability. The advantages of simple preparation, high detection performance, and good regeneration endow the constructed electrochemical sensor with great potential for direct detection of small molecule in complex samples.


Introduction
Vitamins are essential micronutrients for the human body, playing a crucial role in maintaining normal physiological functions.Different vitamins are involved in various physiological processes.For instance, Vitamin B 2 (VB 2 ), also known as riboflavin, is the sole precursor for the biosynthesis of the coenzymes flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) (1)(2)(3).Generally, a deficiency in VB 2 can lead to metabolic disorders, delayed growth in children, skin and mucous membrane lesions, and even diseases such as cancer.On the other hand, excessive intake of VB 2 may cause phototoxic damage to exposed tissues or DNA degradation (4).In Addition, VB 2 also has potential applications in medical treatments, such as photodynamic therapy for destroying tumor tissues, treating cardiovascular diseases (such as stroke), and exhibiting antibacterial properties (5).However, VB 2 cannot be synthesized by the human body and cannot be stored for an extended period within the body (1,2).Therefore, humans and all animals need to continuously obtain VB 2 from food to sustain the metabolic processes involving the abundant coenzymes of VB 2 .As a water-soluble vitamin, VB 2 is widely present in foods such as organ meats, dairy products, whole grains, and egg yolks.However, due to its photosensitivity, a decrease in its content is often considered a sign of food deterioration in food quality monitoring (6-9).Consequently, the development of simple and accurate methods for detecting VB 2 content is of great significance in guiding human nutritional metabolism, supervising the quality of food or drugs, pharmacological research, clinical diagnosis, and the treatment of related diseases.
The current methods for detecting VB 2 mainly based on fluorescence spectroscopy (10,11), chemiluminescence (12), highperformance liquid chromatography (HPLC) (13), and electrochemical sensors (14).However, fluorescence detection is susceptible to background signals from matrix.Chemiluminescence requires high sample purity.Although HPLC is suitable for the analysis of mixtures, it often requires complex pre-treatment, long analysis time and expensive instrument.Electrochemical sensors have advantages such as rapid detection, high sensitivity, ease of miniaturization, and simplicity of instrumentation, making them widely used in analysis of pharmaceutical, food, and clinical samples (15)(16)(17).As a water-soluble substance with three connected aromatic rings and multiple hydroxyl groups, VB 2 is electroactive which undergoes a redox reaction on the electrode involving two protons and two electrons, generating electrochemical signals for detection (18,19).For instance, Gizaw et al. developed a polyglutamic acid-ZnO nanoparticle modified electrode for detecting VB 2 in non-alcoholic beverages and milk samples (20).Lucas et al. constructed an alkylthiolmodified gold electrode, achieving sensitive detection of VB 2 through nonpolar interactions between the alkylthiol end group and VB 2 (21).However, some electrode fabrication steps in these studies are intricate.Moreover, the complex matrix in real samples can affect the analysis.On the one hand, large molecules in samples, such as proteins, can nonspecifically adsorb and occupy active sites on the electrode surface, leading to electrode fouling.On the other hand, other electroactive compounds in the samples can significantly interfere with the signal, reducing the sensitivity, accuracy, and repeatability of electrochemical sensing.Therefore, establishing a simple electrode modification method and enhancing the antifouling/anti-interference abilities are highly desirable.
Among, utilizing porous films to modify electrodes not only optimizes electrode performance but also contributes to its widespread applications in sensing, electrocatalysis, and energy storage (26,27).Firstly, the porous structure provides a larger surface area, increasing the contact area between the electrode and electrolyte, enhancing the reactivity of electrode (28).Secondly, the porous structure helps improve the mass transfer performance, facilitating more efficient transfer of protons or ions between the electrolyte and electrode, improving the reaction kinetics on electrode (29).Additionally, the selectivity of the porous structure can be enhanced by selective controlling pore size and porosity, allowing precise recognition and transfer of specific ions or molecules (30,31).Porous films can also serve as carriers to support the fixation of catalysts or other functional materials, enhancing their stability (32)(33)(34)(35).Among different porous films, vertically-ordered mesoporous silica film (VMSF) possesses unique structures and properties, garnering significant attention in separation and catalysis fields (36)(37)(38)(39)(40). Specifically, VMSF exhibits a highly ordered nanochannel array with high-density (up to 10 12 /cm 2 ), tunable nanochannel diameter (typically ranged from 2 nm to 11 nm), adjustable film thickness (usually 50-200 nm), and excellent chemical stability (41).Due to the ultra-small nanochannels relative to the double-layer thickness or Debye length, VMSF demonstrates selective permeability based on charge and molecular size (42)(43)(44).For instance, the high surface area of VMSF can significantly enhance detection sensitivity by electrostatic and hydrogen bonding interactions, allowing for the enrichment of target analytes or probes (45)(46)(47)(48)(49). Simultaneously, VMSF can mitigate interference from coexisting components in complex matrices through size exclusion and electrostatic repulsion effects (50).For instance, VMSF possesses an insulating silica structure.The ultra-small nanochannels of VMSF can size-exclude large molecules (e.g., proteins, DNA and starch) or particles (e.g., cells, particulate matter), preventing contamination of the underlying electrode (51).On the other hand, the negatively charged surface resulting from ionization of silanol groups can repel common electroactive interferents in complex samples (52).Thus, VMSFmodified electrodes exhibit excellent anti-contamination and interference resistance, holding potential for direct electroanalysis in complex samples.Due to the inert silica structure of VMSF, the selective detection of many electroactive substances by VMSF-based sensors still relies on the electrochemical activity and potential resolution of the underlying electrode.However, except for indium tin oxide (ITO) electrodes, carbon electrodes or noble metal electrodes (gold, platinum) cannot directly achieve stable binding with VMSF.However, ITO electrodes often exhibit a high overpotential when detecting organic electrochemical small molecules.Enhancing the potential resolution and electroactivity of electrodes such as carbon electrodes through convenient electrode modification methods and achieving stable modification of VMSF can effectively expand the application of VMSF-based electrodes in the electrochemical detection of small molecules in complex matrices.
In this work, an electrochemical sensor was conveniently fabricated through a simple and easy-to-operate method, achieving direct and highly sensitive detection of VB 2 in complex samples.The commonly used carbon-based electrode, glassy carbon electrode (GCE), was employed as the supporting electrode.Simple electrochemical polarization was employed to pretreat GCE (p-GCE), endowing the supporting electrode with high peak current, good potential resolution, and the ability to directly and stably bind with vertically-ordered mesoporous silica film (VMSF/p-GCE).Both p-GCE and VMSF 2 Materials and methods

Characterization and instrumentations
The morphology and thickness of VMSF were characterized using an HT7700 transmission electron microscope (TEM) from Hitachi, Japan.The sample preparation involved careful scraping off the VMSF layer from p-GCE using a knife and ultrasonical dispersing it in anhydrous ethanol.The resulting supernatant drops were coated onto a copper grid, dried, and then observed in the TEM chamber at an accelerated voltage of 200 kV.All electrochemical tests were conducted on an Autolab PGSTAT302N electrochemical workstation (Metrotron, Switzerland).The three-electrode system was employed, with the reference electrode being Ag/AgCl (saturated using KCl), the counter electrode being platinum wire or disk (2 cm × 4 cm), and the working electrode being either p-GCE or VMSF/p-GCE.The scan rate in the cyclic voltammetry (CV) test was set at 100 mV/s.For differential pulse voltammetry (DPV) measurements, the parameters including step potential (5 mV), pulse amplitude (25 mV), pulse time (0.05 s), and time interval (0.2 s) were set.

Preparation of p-GCE
Initially, GCE underwent successive polishing with 0.5 μm, 0.3 μm, and 0.05 μm alumina powder.Following the polishing step, GCE surface was subjected to ultrasonic cleaning in ethanol and ultrapure water for 1 min each to remove residual alumina powder, ensuring a cleaner electrode surface.The newly polished GCE was activated using electrochemical polarization under static conditions.Briefly, a phosphate buffer solution (PBS, 0.1 M, pH = 5) served as the electrolyte.The electrochemical polarization involved both anodic oxidation and cathodic reduction (49).Anodic oxidation was carried out using a constant voltage of +1.8 V for 5 min.Subsequently, the obtained electrode underwent cathodic reduction at −1.0 V for 1 min, resulting in the electrochemically pre-activated GCE, referred to as p-GCE.

Fabrication of VMSF/p-GCE
The modification of VMSF on p-GCE electrode was achieved through an electrochemical-assisted self-assembly (EASA) method (53).Initially, a precursor solution was prepared by weighing 1.585 g of CTAB and adding it to a mixed solution consisting of 20 mL ethanol and 20 mL NaNO 3 .The mixture was stirred until completely dissolved.Subsequently, 3,050 μL of TEOS was added as the siloxane source, and the solution was stirred at room temperature for 2.5 h.For VMSF growth, the three-electrode system was immersed in the obtained precursor solution and a constant current (−0.7 mA/cm 2 ) was applied on p-GCE for 10 s.Then, the resulting electrode was promptly removed and immersed in ultrapure water for thorough washing.After aging overnight at 80°C, the electrode with surfactant micelles (SM) blocking the nanochannels was obtained, denoted as SM@ VMSF/p-GCE.Immersing it in a hydrochloric acid/ethanol solution to remove SM from the nanochannels resulted in an electrode modified with an array of open nanochannels (VMSF/p-GCE).

Electrochemical detection of VB 2
When detecting VB 2 with VMSF/p-GCE, a supporting electrolyte solution of PBS (0.1 M, pH = 7.0) was used.Different concentrations of VB 2 were introduced into the electrolyte solution, and the signals generated by the electrochemical oxidation reaction of VB 2 on the electrode were determined using the DPV method.For real sample analysis, VB 2 in milk or composite vitamin B tablets was determined.For milk samples, the samples were diluted 1,000 times with the supporting electrolyte solution before testing.For composite vitamin B tablets, the tablets were firstly ground into powder using a mortar and pestle, and 0.787 g of the powder was dispersed in ultrapure water (2 mL).Then, VB 2 content in the diluted milk or tablet leachate was determined using the standard addition method.

Electrochemical preactivation of GCE and stable integration of VMSF on electrode surface
In this work, a simple electrochemical sensor was efficiently prepared for direct electrochemical detection of VB 2 by integrating VMSF with highly active p-GCE.As illustrated in Figure 1, the widely used GCE was chosen as the supporting electrode.However, GCE can not stably bind with VMSF and its electrochemical activity should be improved.Activating carbon-based electrodes through electrochemical polarization is a simple, green method for preparing highly active electrodes.This method requires no complex chemicals or cumbersome procedures and typically involves electrochemical pretreatment of the electrode in a conventional electrolyte solution.Thus, electrochemical preactivation of GCE through electrochemical polarization was performed to obtain p-GCE with high electrochemical activity and ability to stably bind with VMSF.Herein, electrochemical polarization was performed using anodic oxidation and cathodic reduction sequentially in a PBS solution.Specifically, a higher positive potential (+1.8 V) was applied to GCE for anodic polarization, followed by cathodic reduction at a negative potential (−1 V).During the anodic oxidation process, active oxygen radicals generated from water electrolysis oxidize and etch the sp 2 -conjugated carbon on GCE surface, leading to the formation of abundant edge carbon, defects, and oxygencontaining functional groups (54).X-ray photoelectron spectroscopy (XPS) characterization was employed to investigate the change of the surface chemical groups.The atomic percentages of carbon in GCE, the electrode after anodic polarization, and p-GCE are 14.6, 69.9, and 75.7%, respectively.The corresponding oxygen atomic percentages are 14.6, 30.0, and 24.3% 5%, indicating an increase in oxygen-containing groups on the GCE surface after electrochemical polarization.These functional groups serve as active sites, enhancing the adsorption of organic electroactive molecules on electrode and facilitating interfacial electron transfer reactions.Thus, compared to traditional GCE, p-GCE exhibits higher electrochemical and electrocatalytic activity.
The impact of electrochemical preactivation on electrode properties was examined using the standard redox probe Fe(CN) 6 3−/4− through cyclic voltammetry (CV).As shown in Figure 2A, Fe(CN) 6 3−/4− exhibits a distinct pair of oxidation/reduction peaks on GCE.After anodic polarization of GCE, there are almost no observable oxidation/ reduction peaks on the obtained electrode, indicating that the high positive potential applied during anodic polarization imposes a strong oxidative effect on the electrode surface, inhibiting the electron transfer process at the electrode interface.Subsequently, after cathodic reduction of the electrode, oxidation-reduction peaks of Fe(CN) 6 3−/4− molecules reappear on p-GCE.Compared to GCE, p-GCE demonstrates higher charging current and oxidation-reduction peak current, indicating an increased electrode active area.Moreover, the peak-to-peak separation (66 mV) on p-GCE is smaller than that on GCE (75 mV), suggesting faster charge transfer kinetics for the redox probe on p-GCE.
To study the effect of electrochemical preactivation on potential resolution of electrode, the electrochemical characteristics of common electrochemical small molecules found in biological and food samples, including ascorbic acid (AA), uric acid (UA), and dopamine (DA) on GCE and p-GCE were further investigated.Figure 2B illustrates the differential pulse voltammetry (DPV) curves obtained on GCE and p-GCE in the analysis of the mixture of the three substances.As shown, GCE is unable to differentiate between the three electroactive components, detecting only a single mixed peak with a lower peak current.In contrast, p-GCE is able to distinguish between the three components, exhibiting higher peak currents and superior potential resolution capabilities.This may be attributed to the catalytic effect of oxygen-containing functional groups on the surface of p-GCE, thereby enhancing the electrode's potential resolution capability.
GCE cannot directly and stably anchor VMSF.Processes such as washing after VMSF growth resulted in film detachment.Studies have shown that pre-treating with an organosilane adhesive layer can achieve stable VMSF modification (55).However, this operation increases the complexity of electrode preparation and, on the other hand, a non-conductive adhesive layer can reduce the electrode's electrochemical activity.After preactivation of GCE, the successful integration of VMSF on p-GCE could be achieved without the use of any adhesives.This may be attributed to the formation of-O-Si-O-covalent bonds between the oxygen-containing functional groups on the surface of p-GCE and VMSF.As shown, the electrochemical assisted self-assembly (EASA) method was employed on p-GCE to further integrate VMSF.Currently, the most common methods for VMSF growth are the Stöber solution growth method and EASA method.The former can achieve large-area VMSF preparation on indium tin oxide (ITO) electrode but requires a longer preparation time (over 12 h).On the other hand, EASA method allows for rapid VMSF preparation on various electrodes, typically within 10 s.Based on the size exclusion effect and enrichment of VB 2 of VMSF nanochannels, direct and highly sensitive electrochemical detection of VB 2 in complex samples could be realized.

Integrity, charge-selective permeability, and size exclusion effect of VMSF
By examining the electrochemical performance of two standard redox probes with opposite charges on different electrodes, the integrity and charge-selective permeability of VMSF were investigated.Figures 3A,B show the CV curves obtained on p-GCE, SM@VMSF/ p-GCE, and VMSF/p-GCE electrodes in solutions containing the anionic probe Fe(CN) 6 3− or the cationic probe Ru(NH 3 ) 6

3+
, respectively.Both probes exhibit well-defined oxidation/reduction peaks on p-GCE.When SM@VMSF/p-GCE is used as the working electrode, almost no Faraday current is detected on the electrode.This is attributed to the non-conductive VMSF covering the electrode surface on p-GCE, and the nanochannels of VMSF are filled with SM, keeping nanochannels in a closed state.As a result, redox probe cannot reach the electrode surface through the nanochannels, confirming the integrity of the VMSF film.In marked contrast, when VMSF is directly grown on GCE, the resulting SM@VMSF/GCE exhibits significant oxidationreduction peaks for both probes (insets in Figures 3A,B).This is attributed to the weak binding between GCE and VMSF, causing the film to detach during cleaning processes after growth, thereby failing to completely cover the electrode surface.This also proves the importance of electrode preactivation to achieve stable binding of VMSF without the need for adhesives.Upon removing SM to obtain the VMSF/p-GCE electrode, oxidation/reduction peaks for both probes can be observed again.Compared to p-GCE, the peak current of the negatively charged Fe(CN) 6 3− probe on the VMSF/p-GCE electrode decreases, while the peak current of the positively charged Ru(NH 3 ) 6 3+ probe significantly increases.This demonstrates the charge-selective permeability of the negatively charged surface of VMSF.The silica structure of VMSF is rich in silanol groups (pKa ~ 2), and their ionization in the buffer media results in a negatively charged surface.This leads to electrostatic repulsion toward negatively charged Fe(CN) 6 3− and significant electrostatic attraction for positively charged Ru(NH 3 ) 6 3+ .When detecting complex food or drug samples with an electrochemical sensor, large molecules in the matrix often non-specifically adsorb to the electrode surface, thereby contaminating the electrode surface and affecting the sensitivity and reproducibility of detection.Using VB 2 as the electrochemical substance and bovine serum albumin (BSA) and starch as macromolecular models, the anti-fouling characteristic of p-GCE or VMSF/p-GCE electrodes were investigated.As shown in Figures 4A,B, when BSA or starch is present, the response of the p-GCE electrode to VB 2 is significantly reduced, indicating that these substances tend to contaminate the electrode and reduce the detection signal.In contrast, when these macromolecules are present, the DPV peak current of VB 2 obtained on the VMSF/p-GCE electrode remains almost unchanged, demonstrating the excellent anti-fouling capability.This anti-fouling ability is attributed to the size exclusion effect of the VMSF ultra-small nanochannels.Since these large molecules cannot enter the nanochannels, and the silica structure of VMSF is electrically insulating, BSA or starch can not contaminate the underlying electrode.This gives VMSF/p-GCE an advantage in the direct detection of complex samples.

Morphology characterization of VMSF
The morphology and structure of VMSF were characterized using transmission electron microscopy (TEM).As shown in Figure 4C, the TEM image of VMSF in a top-down view clearly reveals hexagonally arranged mesoporous nanochannels.The film appears uniform, with no observable defects over a large observed area.Figure 4D displayed the TEM image of the cross-sectional section of VMSF with ordered nanochannels with a film thickness (length of nanochannel) of approximately 120 nm.

Feasibility of electrochemical detection of VB 2 and dual signal amplification
The electrochemical behavior of VB 2 on different electrodes was investigated using CV and DPV.ITO electrodes and GCE electrodes were employed as controls.Figures 5A,B depict the CV and DPV curves obtained on various electrodes in VB 2 solution.From the inset in Figure 5A, it can be observed that there are no apparent oxidation or reduction peaks for VB 2 on the ITO electrode.Only very little peak current signal is observed in the corresponding DPV curve (inset in Figure 5B).These results indicates that the ITO electrode is not suitable as a supporting electrode for detecting VB 2 , an organic electroactive molecule.However, when GCE was used for detection, oxidation/reduction peaks of VB 2 appear in the CV, and an oxidation peak near-0.47V is detected in the DPV curve.This is attributed to the presence of aromatic structure in the VB 2 structure, which can adsorb to the electrode surface through π-π stacking interactions, leading to an increase in peak current.For p-GCE obtained after electrode preactivation of GCE, both the oxidation-reduction peaks of VB 2 in CV curve and the oxidation peak current in DPV curve are significantly enhanced.This is because electrochemical preactivation results in a larger electroactive surface area of the electrode, with abundant edge carbon, defects, and oxygen-containing functional groups that can further promote the adsorption and electron transfer reactions of the analyte on the electrode surface through hydrogen bonding or electrostatic interactions.Upon modification of VMSF on the surface of p-GCE, the peak current of VB 2 measured on VMSF/ p-GCE is further increased, demonstrating the significant enrichment effect of VMSF nanochannels on VB 2 .Despite the enrichment effect of nanochannels, the signal of VB 2 on VMSF/ITO electrode is higher than that on ITO electrode but significantly lower than that obtained on VMSF/p-GCE.Thus, both the supporting electrode and the array of nanochannels on VMSF/p-GCE contribute to the enhanced electrochemical signal of VB 2 .

Optimization of electrochemical detection conditions for VB 2
The growth thickness of VMSF on the surface of p-GCE might affect detection sensitivity of VMSF/ p-GCE.As the thickness of VMSF is related to the deposition time in growth (26), the electrochemical signals of electrodes obtained under different VMSF deposition time were investigated in VB 2 solution.As shown in Supplementary Figure S2, the signal of VB 2 increases when VMSF deposition time increases from 2 s to 10s.Subsequently, with a further increase in VMSF deposition time, the electrochemical signal decreases.It is speculated that an excessively thick VMSF layer may compromise the detection performance in two aspects.Firstly, when the VMSF thickness is too thick, the mass transfer of the analyte from the solution to the electrode surface takes a longer time.Secondly, with an excessively long growth time of VMSF, silica nanoparticles might form on the outer surface of VMSF, partially blocking the nanochannels.
To maximize the sensitivity of VB 2 detection on VMSF/p-GCE, the detection conditions were optimized, including the pH of the supporting electrolyte and the VB 2 enrichment time.As shown in Figure 5C, with the increase in pH, the DPV oxidation current of VB 2 on the electrode gradually strengthened, reaching the maximum current response at pH = 7 (Figure 5C).Additionally, as the pH increased, the oxidation peak potential of VB 2 shifted toward negative potentials, indicating the involvement of protons in the electrochemical oxidation-reduction process of VB 2 .From the inset in Figure 5C, it can be observed that the oxidation peak potential (E pa ) of VB 2 has a good linear relationship with CV curves obtained on p-GCE, SM@VMSF/p-GCE, VMSF/p-GCE, and SM@VMSF/GCE (inset) in (A) K 3 Fe(CN) 6 (0.5 mM) or (B) Ru(NH 3 ) 6 Cl 3 (0.5 mM) solution in KHP (0.05 M, pH = 7.4).
pH (E pa = −0.057pH -0.064, R 2 = 0.9991).The slope is close to that of the Nernst equation (59 mV/pH), suggesting that the electrochemical oxidation-reduction process involving VB 2 is an equivalent electron and proton transfer process.Thus, pH = 7 was chosen as the optimal detection condition.Under the optimal pH condition, time for VB 2 enrichment was investigated (Figure 5D).With the increase in enrichment time, the DPV oxidation current of VB 2 on the electrode gradually increased.After VB 2 was enriched for 180 s, the change of current gradually decreased.Considering both the peak current and the detection speed, VB 2 was enriched for 180 s for subsequent experiments.
Faradaic charge is a crucial indicator of electrochemical substances undergoing oxidation-reduction reactions on electrodes.To study the charge transfer of VB 2 on the sensing electrode, the relationship between the oxidation peak current and peak potential of VB 2 with the scan rate (ν) was investigated.As shown in Figure 6A, with the increase in scan rate, the oxidation-reduction peak currents of VB 2 gradually increased, and the peak potential shifted.The oxidation peak current (I pa ) and reduction peak current (I pc ) exhibited a good linear relationship with the scan rate (inset in Figure 6A, I pa = 0.309ν − 1.77, R 2 = 0.9999; Ipc = −0.307ν+ 2.86, R 2 = 0.9988).This indicates that the electrochemical reaction process of VB 2 molecules on the VMSF/p-GCE electrode is adsorption-controlled.The potential of oxidation peak (E pa ) exhibits a well-defined linear relationship with the logarithm of the scanning rate (logν) (Figure 6B), fitting to the following linear equation: Here, E 0 ' is the standard electrode potential, k s is the reaction standard rate constant, D is the diffusion coefficient of VB 2 , α is the electron transfer coefficient of VB 2 , and n α is the electron transfer number.The calculated n α is 2.28, approximately equal to 2, indicating that the reaction is a double electron transfer process.The redox reaction is illustrated in Figure 1.

Electrochemical detection of VB 2
Under optimal conditions, the performance of VMSF/p-GCE in electrochemical detection of VB 2 was investigated.Figure 6C illustrated the DPV curves obtained by VMSF/p-GCE in solutions

Selective, regenerative, and stable performance of the fabricated sensor
When the electrode was used, the complex matrix in real samples might affect the analysis.For instance, large molecules in samples, such as protein, DNA, polysaccharide, can nonspecifically adsorb and occupy active sites on the electrode surface, leading to electrode fouling.This fouling of this electrode will result in changes in electrochemical signals, reducing the accuracy of electrochemical detection.In addition, the co-existed redox molecules might also affect the detection.The selectivity and anti-fouling property of VMSF/p-GCE in detecting VB 2 was investigated.Common metal ions (Na + , Ca 2+ , Mg 2+ , K + ), large molecular, starch, and small molecules present in the human body including ascorbic acid-AA, uric acid-UA, vitamin B 6 , dopamine-DA, glucose-Glu were selected as the possible interfering substances to examine their impact on VB 2 detection.As shown in Figure 7A, the addition of each above substance does not  S3).The regenerative capability of VMSF/p-GCE was also examined.Stirring the electrode in hydrochloric acid-ethanol solution (0.1 M) for 20 min can effectively remove residual VB 2 inside the channels, completing the electrode regeneration.After regeneration, the electrochemical signal measured in the supporting electrolyte was extremely low, indicating the effective removal of VB 2 from the channels during the regeneration process (Figure 7B).The electrode was reused five times for VB 2 detection after regeneration.Compared with the initial detection signal, the obtained peak currents are all higher than 98% with a relative standard deviation (RSD) of 0.5%, demonstrating excellent regenerative capability of the sensor.
The VMSF/p-GCE was stored at room temperature, and VB 2 detection followed by regeneration was performed daily.It was observed in that even after regeneration and storage for 5 days, the peak current measured by VMSF/p-GCE remained at 93.8% of the first-day measurement, proving the electrode's high stability (Figure 7C).

Real sample analysis
To assess the accuracy and precision of the sensor in real sample detection, the standard addition method was employed to detect the VB 2 content in leachate of compound vitamin B tablet and milk samples.As shown in Table 2, the recovery rates ranged from 94.2 to 102%, with a RSD of less than 4.5% for three measurements, demonstrating the accuracy of the detection.Additionally, the VB 2 content in the leachate of compound vitamin B tablet was determined using the extrapolation method and found to be 0.318 μM (as shown    7D), which closely aligned with the value determined by the standard (HPLC GB5009852016).Due to its convenient preparation, simple operation, and the absence of complex sample pretreatment, the developed electrochemical sensor has significant potential for direct electroanalysis of VB 2 in complex samples.

Conclusion
In summary, VMSF modified carbon-based electrode was fabricated through a simple and rapid method for highly sensitive detection of vitamin B 2 .Utilizing electrochemical preactivation of GCE, a p-GCE with excellent potential resolution and high electrochemical response was prepared.Using p-GCE as the supporting electrode, VMSF is directly grown via a rapid EASA method, eliminating the need for any adhesives and ensuring the stable integration of VMSF.Due to the dual enrichment of the supporting electrode and nanochannels, VMSF/p-GCE exhibited high sensitivity for electrochemical detection of VB 2 .Owing to the potential resolution of p-GCE and selectivity permeability of VMSF nanochannel, VMSF/p-GCE exhibits good selectivity and high antifouling performance.Direct electrochemical detection of VB 2 in turbid samples without the need for complex sample pretreatment is realized.The sensor constructed in this study has advantages including simple fabrication, high sensitivity, good selectivity and excellent regeneration, holds promising applications in direct electroanalysis of VB 2 in real samples.If miniaturized electrodes or electrochemical detection devices controlled by smartphones are used, portable VB 2 detection is expected to be carried out.The real samples were first spiked with the targets VB2 and then diluted with the supporting electrolyte solution by a factor of 100.The indicated concentration was obtained after dilution.
. The changed atomic percentages indicates the changed chemical structure on the electrode surface.Supplementary Figure S1 displays high-resolution C1s spectra of GCE, the electrode after anodic polarization, and p-GCE.As shown, all electrodes have four carbon bonds including C-C/C=C(sp 2 C, 284.4 eV), C-O (285.8 eV), C=O (287.1 eV), and O-C=O (288.6 eV).After anodic polarization, the content of C=O and O-C=O bonds on electrode surface significantly increased, while the content of C-O bonds decreased, suggesting an increased oxidation degree of carbon atoms under high potential.Following the subsequent cathodic reduction, the content of C-O bonds increased, and the content of C=O bonds decreased significantly, indicating the reduction of C=O bonds to C-O bonds.Compared to GCE, the C-O bond content on p-GCE increased from 21.4 to 24.

FIGURE 1
FIGURE 1Illustration for the fabrication of VMSF/p-GCE for electrochemical detection of VB 2 .

FIGURE 4
FIGURE 4 Stability of the VMSF/p-GCE.Normalized peak current ratio on VMSF/p-GCE or p-GCE toward VB 2 (5 μM).I and I 0 represent the currents obtained in the present and absence of 5 μg/mL of (A) Starch and (B) BSA in PBS (0.1 M, pH = 7).The insets are the corresponding DPV curves obtained for the p-GCE and VMSF/p-GCE in the absence and presence of starch or BSA.Top-view (C) and cross-sectional (D) TEM images of VMSF at different magnifications.

FIGURE 5 (
FIGURE 5 (A) CV curves and (B) DPV obtained on different electrodes in PBS (0.1 M, pH = 7.0) containing VB 2 (5 μM).The scanning rate is 100 mV/s.The enrichment time is 180 s.Inset is the DPV obtained on ITO and VMSF/ITO under the same conditions.(C) CV curves obtained at 100 mV/s on VMSF/p-GCE in the presence of 5 μM VB 2 in PBS (0.1 M) at various pH values.Inset is the linear dependence of E pa on pH value.(D) The peak current obtained on VMSF/p-GCE at different accumulate time.

FIGURE 6 (
FIGURE 6 (A) CV curves obtained at different scan rates for VMSF/p-GCE in PBS (0.1 M, pH 7.0) containing 5 μM VB 2 .The inset is the dependence of I pa on scan rate.The enrichment time is 180 s.(B) The dependence of the redox peak potentials of 5 μM VB 2 on log of scan rate.(C) DPV curves obtained on VMSF/ p-GCE in PBS (0.1 M, pH = 7.0) containing different concentrations of VB 2 .The inset is the magnified view of the DPV curves in the low concentration region.(D) The calibration curve for VB 2 .The inset in (D) shows the calibration lines in the low concentration range.

FIGURE 7 (
FIGURE 7 (A) The current ratio (I/I 0 ) obtained on VMSF/p-GCE for detection of VB 2 (5 μM) in the absence (I 0 ) and presence (I) of 5 μM (Na + , Ca 2+ , Mg 2+ , K + , VB 6 , Glu, DA, Starch, AA) of other added interfering species.(B) The reuse performance of VMSF/p-GCE.The peak currents are obtained in electrolyte (bottom) or VB 2 solution (top) using the regenerated electrodes.(C) Stability of the VMSF/p-GCE.The currents of the electrodes in the presence of VB 2 (5 μM) over a 5-day storage period.After each measurement, the electrode was regenerated.(D) The standard curve for detection of VB 2 in milk or leachate of compound vitamin B tablet using standard addition method.Inset is the image of leachate of compound vitamin B tablet (left) or milk (right) sample.

TABLE 2
Determination of VB 2 in leachate of compound vitamin B tablet or milk.