Association between CLN3 (Neuronal Ceroid Lipofuscinosis, CLN3 Type) Gene Expression and Clinical Characteristics of Breast Cancer Patients

Breast cancer is the most common cancer in women worldwide. Elucidation of underlying biology and molecular pathways is necessary for improving therapeutic options and clinical outcomes. CLN3 protein (CLN3p), deficient in neurodegenerative CLN3 disease is anti-apoptotic, and defects in the CLN3 gene cause accelerated apoptosis of neurons in CLN3 disease and up-regulation of ceramide. Dysregulated apoptotic pathways are often implicated in the development of the oncogenic phenotype. Predictably, CLN3 mRNA expression and CLN3 protein were up-regulated in a number of human and murine breast cancer-cell lines. Here, we determine CLN3 expression in non-tumor vs. tumor samples from fresh and formalin-fixed/paraffin-embedded (FFPE) breast tissue and analyze the association between CLN3 overexpression and different clinicopathological characteristics of breast cancer patients. Additionally, gene expression of 28 enzymes involved in sphingolipid metabolism was determined. CLN3 mRNA is overexpressed in tumor vs. non-tumor breast tissue from FFPE and fresh samples, as well as in mouse MCF7 breast cancer compared to MCF10A normal cells. Of the clinicopathological characteristics of tumor grade, age, menopause status, estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 (HER2), only absence of HER2 expression correlated with CLN3 overexpression. Sphingolipid genes for ceramide synthases 2 and 6 (CerS2; CerS6), delta(4)-desaturase sphingolipid 2 (DEGS2), and acidic sphingomyelinase (SMPD1) displayed higher expression levels in breast cancer vs. control tissue, whereas ceramide galactosyltransferase (UGT8) was underexpressed in breast cancer samples. CLN3 may be a novel molecular target for cancer drug discovery with the goal of modulation of ceramide pathways.


Introduction
Globally, about 1.38 million women are diagnosed with breast cancer and 458,503 die from the disease every year (1). The incidence is rising in low-income and middle-income countries, a trend attributed to improved life expectancy, urbanization, and adoption of a Western diet and lifestyle (2)(3)(4). In Lebanon, breast cancer is now the leading cancer among women (5,6) and contributes to 45% of annual registered deaths (5). Breast cancer in Lebanon manifests larger tumor size, more lymph node involvement, and higher tumor grades (7,8).
CLN3p impacts cell-cell communication, apoptosis and autophagy, proper functioning of lysosomes, and galactosylceramide (GalCer) lipid transport from Golgi to lipid rafts in the plasma membrane (9). Defects in this gene lead to Juvenile Neuronal Ceroid Lipofuscinosis or CLN3 disease, a pediatric neurodegenerative disease (10). In CLN3 disease, defective or low levels of CLN3p lead to failure of GalCer transport to lipid rafts, thus impairing lipid raft function, and resulting in toxic increases in ceramide (9). This process leads to enhanced apoptosis of neurons and photoreceptors initiated via caspase-8 activation (9,11).
Cancer and neurodegeneration are often two sides of the same coin. Low levels of CLN3p lead to neurodegeneration and enhanced apoptosis, and high levels of CLN3p may lead to inhibition of apoptosis, cellular proliferation, and carcinogenesis. The expression of CLN3 mRNA and CLN3 protein is increased in a variety of cancers, including prostate, ovarian, colon, glioblastoma, and, human breast cancer-cell lines and solid colon cancer ( Figure S1 in Supplementary Material) (12). High levels of CLN3 are linked to inhibition of apoptosis by negatively impacting ceramide generation upstream (11,13). Moreover, blocking CLN3 expression using anti-sense strategies led to cancercell killing, suggesting CLN3p may be a potential therapeutic target (12).
There is increasing evidence for the involvement of sphingolipid signaling in breast cancer (14). Sphingolipids are a family of lipids lending structural support to the membrane bilayer (15). Also, sphingolipid-regulated functions have significant and specific links to various aspects of cancer initiation, progression, and response to anticancer treatments. Ceramide, in particular, is intimately involved in the regulation of cancer-cell growth, differentiation, senescence, and apoptosis (16).
In this study, levels of CLN3 expression in 75 paired invasive ductal carcinomas (IDC) and their corresponding non-tumor freshly dissected breast tissues and another 189 breast cancer tissue paraffin blocks and corresponding normal tissue paraffin blocks from the same patient are compared by quantitative realtime polymerase chain reaction (qRT-PCR). The association of CLN3 expression with specific clinicopathological characteristics of breast cancer patients is documented. In addition, gene expression of enzymes controlling sphingolipid metabolism in fresh-frozen breast cancer tissues is established, confirming the CLN3-ceramide link.

Specimen Collection
A total of 75 patients with IDC of the breast were subjected to surgical resection at the American University of Beirut Medical Center, between September 2012 and May 2014. Fresh breast cancer and corresponding non-tumor breast samples were collected and stored in RNAlater ™ (Qiagen) at −20°C. AUB-IRB (American University of Beirut -Institutional Review Board) approved our study (IRB number: IM.AT.05, last updated on May 27th, 2013). Written informed consent was obtained from patients following this AUB-IRB-approved protocol, and in accordance with the Declaration of Helsinki. Additionally, formalin-fixed/paraffinembedded (FFPE) breast tissue blocks from 7 patients who underwent reduction mammoplasty and from 189 patients (paired IDC and their corresponding non-tumor breast tissue blocks) were obtained from the Department of Pathology of the American University of Beirut Medical Center, also under an AUB-IRB-approved protocol. All samples were used for detection of CLN3 mRNA expression. Patient clinicopathological characteristics were tabulated, including tumor grade, age, menopause, estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) status.

mRNA Expression Levels
Formalin-fixed/paraffin-embedded tissue samples were cut into 20 μm × 10 μm sections to be included per extraction. FFPE sections were deparaffinized using xylene and lysed overnight in proteinase K. Then, mRNA was extracted using the RiboZol ™ RNA Extraction Reagent (Amresco) according to the manufacturer instructions. RNA was eluted in nuclease-free water and stored at −80°C until use. RNA was treated with DNase enzyme (Fermentas) to remove genomic DNA. Using RNeasy ® Plus Mini Kit (Qiagen), total RNA was extracted from human fresh breast tissues, according to the manufacturer's protocol. For assessing RNA quality and yield, A 260 /A 280 and A 260 /A 230 ratios for RNA were analyzed with a NanoDrop ® ND-1000 spectrophotometer (NanoDrop Technologies) and Experion ™ Automated Electrophoresis System (BioRad), respectively.
RNA was reverse transcribed using RevertAid Reverse Transcriptase (Thermo Scientific) with 100-1000 ng of input RNA and random primers (Thermo Scientific). Quantitative real-time PCR reactions were performed in 96-well plates using specific primers (TIB MOLBIOL) and the iQ ™ SYBR ® Green Supermix (BioRad) as a fluorescent detection dye, in CFX96 ™ Real-Time PCR (BioRad), in a final volume of 12.5 μl. To characterize generated amplicons and to control contamination by unspecific byproducts, melt-curve analysis is applied. Each reaction was performed in duplicate. All results were normalized to PGK1 mRNA level and calculated using the ΔΔC T method. The specificity of the PCR was determined by melt-curve analysis for each reaction. Primer sequences are listed in Table S1 in Supplementary Material (Tm = 60°C).

siRNA CLN3 Knockdown
siRNA for CLN3 knockdown (BLOCK-iT RNA ™ Designer, Invitrogen) or scrambled control were transfected into MCF7 cells (HiPerfect Transfection, Qiagen). Cell pellets are collected on day 4 to measure ceramide levels using the diacylglycerol kinase (DGK) assay. For validation of CLN3 knockdown, quantitative real-time PCR reactions were performed. Results were normalized to Cyclophyllin A mRNA level and calculated using the ΔΔC T method. Primer sequences are listed in Table S1 in Supplementary Material (Tm = 60°C).

Trypan Blue Dye Exclusion Method
Cell growth and viability was determined using trypan blue dye exclusion. About 100,000 MCF7 cells are seeded per well and transfected with CLN3 siRNA/scrambled siRNA. After 96 h, cells were washed, centrifuged, and fresh media added. The cells are then stained with trypan blue dye (0.4%) and the number of white viable and dead blue cells is counted using a light microscope and a hemocytometer.

Propidium Iodide Staining
About 100,000 MCF7 cells are transfected with CLN3 siRNA/scrambled siRNA, suspended in 100 μl PBS, incubated with 100 μl/0.5 mg/ml PI, washed, placed on slides, and viewed with a fluorescent microscope. Cells are harvested 72 and 96 h after transfection. Three hundred cells in three different fields of vision were counted, and percentage of red staining apoptotic cells/total cells determined.

Measurement of Ceramide Levels Cell Homogenization
Cell pellets were collected and washed with PBS, then suspended in methanol/chloroform (2:1) and stored at −80°C for 48 h.

Lipid Extraction
Seven hundred microliters of distilled water are added to the homogenate, then 1 ml chloroform and 1 ml of distilled water, and the sample centrifuged for 10 min at 4°C. The lower phase is lyophilized. Lipids are resuspended in 1 ml chloroform.

Ceramide Assay
Ceramide standards and samples are dried using a speedvac. Micelles were added to samples/standards and sonicated for three cycles of 30 min each. Reaction mixtures are added to the ATP mix and incubated at RT for 45 min. The reaction is stopped using methanol/chloroform/distilled water, then lipids extracted. These are run on a TLC plate using chloroform/acetone/methanol/acetic acid/water (50:20:15:10:5). Plates are then dried, x-ray film overlaid and kept at −80°C overnight. Ceramide bands are visualized, scraped into scintillation vials, and counts per minute detected using a liquid scintillation counter. The results are expressed as picomoles of ceramide per nanomole of total phospholipids.

Phosphate Determination
After lyophilization, 150 μl of 70% perchloric acid are added to samples and to disodium hydrogen phosphate (Na 2 HPO 4 ) standards. Tubes are capped with glass balls previously soaked in methanol and placed at 180°C for 1 h, cooled at RT and distilled water/2.5% ammonium molybdate/10% ascorbic acid added. Mixtures are incubated for 15 min at 50°C, and concentration determined with a spectrophotometer (820 nm wavelength).

Microarray Expression Profiling and Data Analysis
Gene expression analysis was performed on 94 fresh breast tissue samples (84 cancerous and 10 normal) using the GeneChip Human Genome U133 Plus 2.0 arrays (Affymetrix Inc.) representing over 45,000 transcripts. Samples were prepared and microarrays were processed using the GeneChip 3 ′ IVT Express kit as instructed by the manufacturer. Briefly, 100 ng of total RNA are fragmented then hybridized to the arrays. After washing and staining using the GeneChip Fluidics Station 450, the arrays are scanned with the GeneChip Scanner 3000 7G. Cell intensity data (CEL) files are generated with the Affymetrix GeneChip Command Console (AGCC) software version 3.2.
Data were analyzed within the R statistical environment (17) using Bioconductor (http://www.bioconductor.org) packages. Stringent quality control criteria were applied to the data. Three samples were found to be of a low quality and were excluded of subsequent analyses. Data were normalized jointly with the Robust Multiarray Average (RMA) algorithm (18).
Where more than one probe was found to match the same gene the mean expression value was calculated and used. Expression boxplots for the genes of interest of tumor against normal samples were generated from this expression matrix.
To detect differentially expressed genes between tumor and normal samples, Limma (19) was used to fit a linear model to normalized expression data for each probe. False discovery rates (FDRs) were estimated using the Benjamini-Hochberg method (20).

Statistical Analysis
CLN3 mRNA expression was quantified in breast adenocarcinomas and overexpression was defined as a 1.25-fold increase in expression compared to normal tissue. Continuous data were expressed as means ± SEM, whereas categorical data were summarized as counts and percents. The chi-square (χ 2 ) test for categorical variables was used to determine the association between gene expression levels and clinicopathological characteristics. Means of continuous data were compared by the two-tailed Student's t-test. For multiple group comparisons, two-way ANOVA followed by Bonferroni's correction was used. Gene expression data derived by Affymetrix or real-time PCR were compared for tumor vs. normal tissue by standard t-test. The concordance or discordance between normal breast tissue adjacent to the tumor and normal breast tissue from reduction mammoplasties was analyzed using the kappa (κ) test of concordance. Concordances of <0.40, 0.40-0.75, and >0.75 were defined as poor, moderate, and perfect, respectively. SPSS statistical software version 21.0 (SPSS Inc., Chicago, IL, USA) was used for analysis, except for the Affymetrix expression data that were analyzed using R statistical environment. All tests were two-sided and p < 0.05 was considered as a statistically significant difference.

Patient Characteristics
Of the 189 patient malignant FFPE tissues from breast adenocarcinomas, only 175 had corresponding non-tumor FFPE tissue. Of these 175 patients, 21, 49, 51, and 54 patients had Ductal Carcinoma In Situ (DCIS), or IDC grades I, II, and III, respectively. A total of 26 were <40 years, 56 were between 40 and 49 years, 66 were between 50 and 69 years, 22 were >70 years old. In five patients, age was undetermined. Eighty-three were menopausal, 87 were premenopausal and menopausal status was undetermined in 5 patients. ER was positive in 128 patients, negative in 31. Information on another 16 patients was not available. PR was positive in 105, negative in 51, and undetermined in 19 patients. Thirty-two were HER2 positive, 108 were HER2 negative and information on an additional 35 patients was not available.
The malignant FFPE tissue from 189 patients was compared to non-tumor FFPE tissue collected from 7 reduction mammoplasties. Of those, 22, 50, 57, and 60 patients had DCIS, and IDC grades I, II, and III, respectively. A total of 29 were <40 years, 58 were between 40 and 49 years, 68 were between 50 and 69 years, 28 were >70 years old, whereas in 6 patients, age was undetermined. Ninety-one were menopausal, 92 were premenopausal and menopausal status was undetermined in 6 patients. ER was positive in 139 and negative in 33 patients. Information on 17 patients was not available. PR was positive in 114, negative in 55 and undetermined in 20 patients. Thirty-five were HER2 positive and 117 patients were HER2 negative. Information on 37 patients was not available. Clinical characteristics of the patients are summarized in Table 1.
Patient clinicopathological characteristics and their frequencies from IDC of the breast and non-tumor tissue (from same patient or from reduction mammoplasties) were compared. The table also displays the frequency distribution and percentage of the two variables: CLN3 overexpression and patient clinicopathological characteristics. Statistics were calculated by the Pearson χ 2 test and undetermined samples were not included. CLN3 expression levels were analyzed by comparing malignant tissue from breast adenocarcinomas to corresponding non-tumor tissue. The cut-off point for CLN3 overexpression was a 1.25-fold increase. A p < 0.05 was considered as statistically significant.

CLN3 Gene Expression
To determine the potential role of CLN3 in the development and progression of IDC of the breast, 75 paired fresh tumor tissues and 189 paired FFPE IDC breast samples were collected and When comparing IDC breast samples to non-tumor tissue from reduction mammoplasty patients, overexpression of CLN3 was most prevalent for IDC grades I and II (54%), and 50% in DCIS and also 50% in IDC grade III. There is an increase in percentages of CLN3 overexpression when tumor tissue is compared to tissue from reduction mammoplasties as opposed to normal tissue from the same patient removed at surgery (Figure 1A). Also in fresh tissue, 50% of IDC grade I cases showed overexpression of the CLN3 gene. Prevalence of CLN3 overexpression is highest in IDC grade II (67%), followed by a decrease in IDC grade III (44%). Note the difference in percentages of CLN3 overexpression in FFPE tissue (49, 41, and 35%) for IDC grades I, II, and III, respectively. Higher percentages of CLN3 overexpression are observed in fresh breast tissue, as compared to FFPE tissue ( Figure 1B).

CLN3 Gene Expression Levels and Clinicopathological Characteristics
CLN3 overexpression did not correlate with lower or higher tumor grade, age, menopausal status, ER, PR, and HER2 expression, when comparing the level of CLN3 mRNA between breast cancer and the surrounding non-tumor tissue ( Table 1).
When comparing cancerous tissue to non-tumor tissue from reduction mammoplasties, no positive correlation was observed with respect to CLN3 overexpression and higher tumor grades, age, and menopause status. Similarly, there was no significant difference in CLN3 overexpression between patients with negative or positive ER and PR status. CLN3 overexpression in patients with no HER2 expression was significantly higher compared to those with positive HER2 breast cancer (p = 0.045) ( Table 1). In fact, the cross-tabulation displaying the frequency distribution of the two variables (CLN3 mRNA expression and HER2 status) shows that when HER2 is not expressed, CLN3 is overexpressed in 66/117 (56%) of cases, whereas, when HER2 is overexpressed, CLN3 is overexpressed in only 13/35 (37%) of cases (Table 1). So, 56% of breast cancer patients that exhibit overexpression of CLN3 mRNA lack HER2 expression compared to only 37% overexpressing HER2 ( Figure S2A in Supplementary Material). Fifteen percent of those cases overexpressing CLN3 and lacking HER2 receptor are triple negative (HER2/ER/PR negative) and 85% are ER/PR positive ( Figure S2B in Supplementary Material).

Growth, Apoptosis Rate, and Ceramide Levels in CLN3-Deficient MCF7 Cells
CLN3 mRNA is overexpressed 3.5-fold in MCF7 cells compared to MCF10A cells, making MCF7 cells an excellent in vitro model to study the impact of CLN3 expression in breast cancer (Figure 2A). A significant blocking of CLN3 protein expression using CLN3 Frontiers in Oncology | www.frontiersin.org October 2015 | Volume 5 | Article 215 siRNA was achieved in MCF7 cells. Blocking CLN3 expression inhibited growth and viability of MCF7 cancer cells (Figure 2B), and increased apoptosis as shown by PI staining (Figure 2C). Previous work had shown that CLN3 negatively modulates ceramide generation in NT2 cells that are human teratocarcinoma-derived progenitor cells (21). Threefold higher levels of endogenous ceramide were observed after MCF7 cells were transfected with siRNA directed against CLN3 compared with control MCF7 cells transfected with scrambled siRNA (Figure 2D).

Validation of Microarray Analysis Results with qRT-PCR
The differential expression of the six selected genes was examined using qRT-PCR: CerS2, CerS6, DEGS2, SMPD1, CLN3, and UGT8.
Results exhibited high consistency with those of the microarray analysis. Tumor status induced a significant increase in the expression of CerS2 (p < 0.0001), CerS6 (p < 0.0001), DEGS2 (p < 0.0001), SMPD1 (p < 0.05), and CLN3 (p < 0.0001); and a significant decrease in the expression of UGT8 (p < 0.0001) compared with expression in the corresponding non-tumor tissue ( Figure 3B).

Discussion
In this study, CLN3 mRNA expression levels in breast cancer patient FFPE and fresh tissue samples were determined and association with patient characteristics established. The relative overexpression of CLN3 mRNA transcripts in IDC breast FFPE and fresh tissues was significantly higher than in surrounding control non-tumor tissues or in control non-tumor tissue from reduction mammoplasties. Furthermore, of the six clinicopathological parameters studied (tumor grade, age, menopause status, ER, PR, and HER2), CLN3 overexpression was significantly associated with absence of HER2 expression (p = 0.045) in patients with IDC of the breast ( Table 2).
Receptor status is used as a guide to classify breast cancers into four biological subtypes: (a) low grade luminal A subtype, (b) high grade luminal B subtype (High grade, ER/PR positive or HER2+/ER+/PR+), (c) HER2 subtype (HER2+/ER−/PR−), and (d) triple negative/basal like subtype (HER2−/ER−/PR−) (22). So, the subtypes that are negative for HER2 receptor are luminal A, accounting for approximately 40% of all breast cancers and triple negative/basal like tumors that represent 10-20% of breast cancers and are negative, for all receptors. Lack of receptors limits the use of targeted treatments, such as hormonal therapy and anti-HER-2 agents, leading to a high proportion of disease-related death. The Human HER2 oncogene, also known as neu/c-erbB2, is responsible for encoding a 185-kDa transmembrane receptor tyrosine kinase overexpressed in 20-30% of breast cancers and is associated with increased breast cancer recurrence and a worse prognosis (23). It is a member of the epidermal growth factor (EGF) receptor (EGFR) family. The use of trastuzumab (Herceptin ® ), a humanized monoclonal antibody to the HER2 extracellular domain, improves survival in both early and metastatic HER2positive patients (24,25). HER2 expression in breast cancer is associated with poor prognosis (26). Elevated expression of the CLN3 gene in breast cancer was significantly associated with HER2-negativity, when comparing cancerous to non-tumor tissue from reduction mammoplasties. Interestingly, only 15% of the cases overexpressing CLN3 and lacking HER2 receptor are triple negative (HER2/ER/PR negative) and 85% are ER/PR positive, indicating that up-regulated CLN3 could be a good prognostic factor in HER2-negative breast cancers, suggesting a broad application of CLN3 targeted therapies in breast cancers that do not overexpress HER2. To address the extent to which CLN3 could be a prognostic factor in breast cancer, the correlation between CLN3 expression and clinical disease outcomes, such as survival, recurrence, and metastasis, in different subtypes of breast cancer, would still need to be assessed. Defects or diminished CLN3 protein expression in the juvenile form of CLN3 disease is associated with increased levels of the pro-apoptotic sphingolipid ceramide in brains and cells from these patients. Sphingolipids are bioactive lipids that play roles in the structure and regulation of cellular membranes, and also impact signaling acting as protagonists in neurodegenerative disease, inflammatory disease and cancer (27). Alterations in sphingolipid metabolism also contribute to chemoresistance and tumor survival (28). The central character in the sphingolipid pathway is ceramide. It is either synthesized de novo from serine and palmitate or generated by breakdown of sphingomyelin via different sphingomyelinases (29) (Figure 4). Of the different functions attributed to ceramide, special attention has been given to its pro-apoptotic properties (30), and its significance as a potential target for cancer chemotherapy (31). Previous work has shown that CLN3 is anti-apoptotic, and that diminishing levels of CLN3 protein in cancer cells enhances ceramide production and results in death of cancer cells (21). Ceramide is also produced in response to stress stimuli, including some chemotherapeutic drugs and irradiation (16). The cell death function of ceramide suggests that ceramide analogs may open doors to new therapies to battle cancer. Thus, finding ways to increase ceramide by exogenous treatment or by elevating endogenous ceramide in cancer cells becomes desirable. The role of sphingolipid signaling in HER2-negative breast cancer, however, is not yet well-defined.
We investigated whether a correlation exists between several elements of sphingolipid metabolism and tumor status in breast cancer in fresh tissue samples. Significant differences in tumor vs. control non-tumor tissue in the expression of many genes involved in this pathway were found. Ceramide synthase 2 (CerS2), ceramide synthase 6 (CerS6), delta(4)-desaturase, sphingolipid 2 (DEGS2), and acid sphingomyelinase (SMPD1) displayed higher expression in tumor samples. The alteration of these four enzymes may have several implications. First, dihydroceramide synthases (CerS2 and CerS6) acylate sphinganine and sphingosine to form dihydroceramide and ceramide, respectively (Figure 4). Erez-Roman et al. also determined that CerS2 and CerS6 mRNA expression is higher in human breast cancer tissue compared to paired normal tissue from the same patients (32). The hypothesis that higher levels of dihydroceramide synthases would directly or indirectly lead to higher levels of ceramide in tumor tissue may be an attempt to achieve a more efficient killing of cancer cells. The third enzyme dihydroceramide destaurase (DEGS) is responsible for the conversion of dihydroceramides generated via de novo biosynthesis to ceramides by the incorporation of a 4,5trans-double bond, a process occurring within the endoplasmic reticulum (33) (Figure 4). The indirect augmentation of dihydroceramides by siRNA blockade of DEGS1 or DEGS2 decreased cell proliferation (34). Inhibition of DEGS1 with siRNA in human neuroblastoma cells leads to the accumulation of endogenous dihydroceramides with subsequent effects on cell growth, particularly cell cycle arrest (35). The enzyme acid sphingomyelinase (SMase) catalyzes hydrolysis of sphingomyelin to ceramide and phosphocholine (Figure 4) (38). Ceramide galactosyltransferase (UGT8) showed lower expression in tumor tissue, but there is yet little information regarding its impact on cancer development. UGT8 encodes an endoplasmic reticulum-localized enzyme responsible for synthesis of galactosylceramide (GalCer) from ceramide (39). Altogether, the significant differences in the expression of CerS2, CerS6, DEGS2, SMPD1, and UGT8 sphingolipid genes in tumor tissue go hand in hand with elevation of ceramide levels (Figure 4).
Ceramide is a well-established growth-inhibitory molecule, and up-regulation of growth-inhibitory molecules in human malignancies is not without precedence. Tumor necrosis factor-related apoptosis-inducing ligand is up-regulated in colon cancer, and this increase is associated with higher-grade tumors and a poorer prognosis (40). It is more plausible that up-regulation of specific sphingolipid enzyme mRNA expression in tumors could be a positive compensatory response by the cell as an attempt to increase ceramide production ( Figure 5). Breast tumorigenesis is a multistep process going from benign and atypical hyperproliferation, to in situ carcinoma, invasive carcinomas, and culminates in metastatic disease (41). The development and progression of breast cancer is a complex process attributable to the interaction of many genetic, epigenetic, and environmental factors. Altered gene expression profiles may drive disease progression (42). Breast cancer-overexpressed gene 1 (BCOX1) mRNA transcripts are detected in breast cancer tissues, and also, albeit at lower levels, in the corresponding non-tumor breast tissues (43). Hence, it is possible that CLN3 may also be overexpressed in non-tumor tissue surrounding the tumor. Discrepancy between CLN3 mRNA expression in non-tumor tissue surrounding the tumor and non-tumor tissue from reduction mammoplasties was calculated using the concordance correlation coefficient. There was poor concordance between non-tumor tissue surrounding the tumor and nontumor tissue from reduction mammoplasties (κ = 0.115) indicating that CLN3 gene expression in "morphologically" normal tissue derived from breast cancer patients is different from that of healthy individuals undergoing breast reduction surgery. Non-tumor breast tissue from cancer patients, although normal at the clinical pathological level, is diseased at the molecular level. This finding carries important clinical consequences. After surgical removal of a tumor, there is still a high risk for tumor to develop in the same anatomical area. The new tumor has been traditionally explained by the growth of incompletely resected carcinomas. For cases where the tumor had been completely removed, according to pathology parameters, a genetically altered field at the molecular level remains and may be the cause of new cancer. The presence of a field with molecularly altered cells appears to be a risk factor for cancer. So, our results confirm the concept of field cancerization. In fact, data are widely available that cancer can develop from genetically altered cells in a field that was left behind in the patient after surgical removal of the initial carcinoma (44,45).
In clinical practice, the elucidation of the association between gene expression profiles and clinicopathological characteristics may aid physicians in selecting patient-suitable treatments. Our findings confirm an association between high expression levels of the CLN3 gene and absence of HER2 expression in breast cancer patients, which increases the possibility of including it as a biomarker in breast cancer diagnostics. Good biomarkers can aid early cancer screening, confirm cancer diagnosis, predict outcome, tailor therapy, and guide future research directions by shedding light on new tumorigenesis pathways. The tools currently available to assess risk of progression of pre-invasive lesions to invasive ones are largely based on epidemiological data and patterns of progression across populations, with little guidance regarding individual risks for tumor progression. Fonsesca et al.
show that in pre-invasive breast lesions, such as DCIS, the individual's risk of progression to IDC is unclear, yet surgery and other therapies seem to decrease the risk of invasive cancer (46). Also, in prostate and colon, pre-invasive lesions are much more common than aggressive cancers, and only 10% or less develop into invasive ones (47). Surgical and other interventions, now considered standard of care, might be excessive in a subset of lesions not destined to progress into aggressive phenotypes. Biomarkers can help select patients requiring intervention, and may allow targeted strategies to be developed for prevention of tumor progression.
A recent study demonstrated that acid ceramidase upregulation (a ceramide-metabolizing enzyme), is a conserved response to radiation therapy across multiple tumor types and acid ceramidase inhibition can directly improve the clinical response to radiotherapy in vitro and in vivo (48).
In conclusion, the expression of CLN3 is increased in human breast cancer. Moreover, several enzymes from the de novo ceramide synthesis pathway are differentially expressed in breast cancer, implicating ceramide and its upstream regulator, the CLN3 gene, in breast cancer.
CLN3 expression may be an additional useful biomarker and a novel molecular target for cancer drug discovery, the latter achieved via modulation of ceramide pathways.