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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Oncol. | doi: 10.3389/fonc.2019.00332

YTHDF1 Regulates Tumorigenicity and Cancer Stem Cell-Like Activity in Human Colorectal Carcinoma

 Yang Bai1,  Yi Zhang1*, Chunxing Yang1, Runliu Wu1, Shenlei Song1, Wanwan Li1, Peichen Yan1, Lihua Huang1, Changwei Lin1 and Daojiang Li1
  • 1Third Xiangya Hospital, Central South University, China

YTH N6-methyladenosine(m6A) RNA binding protein 1 (YTHDF1) is a core factor in RNA methylation modification. Recent studies have shown that m6A is closely related to multiple tumors, thus YTHDF1 may also play a role in tumorigenesis. This study, aimed to explore the role of YTHDF1 in the colorectal cancer (CRC) . In this study, we identified YTHDF1 as being highly expressed at the mRNA and protein levels in TCGA, GEO CRC and primary CRC. Furthermore, the YTHDF1 gene copy number was positively correlated with YTHDF1 mRNA expression in CRC. Knocking down the expression of YTHDF1 significantly inhibited the CRC cell’s tumorigenicity in vitro and murine xenograft tumor growth in vivo. Furthermore, silencing of YTHDF1 inhibited the colonosphere formation ability in vitro. Mechanistically, we found that silencing YTHDF1 significantly inhibited Wnt/β-catenin pathway activity in CRC cells. Together, YTHDF1 is overexpressed in CRC and plays a vital oncogenic role in CRC, and this novel finding may provide a potential therapeutic target for CRC.

Keywords: CRC, YTHDF1, CNV (Copy Number Variant), CSC - cancer stem cell, Wnt / beta-catenin, Tumorigenecity

Received: 04 Jan 2019; Accepted: 11 Apr 2019.

Edited by:

Massimo Bonora, Department of Cell Biology, Albert Einstein College of Medicine, United States

Reviewed by:

Hamid MORJANI, Université de Reims Champagne-Ardenne, France
Dong-Yan Shen, Xiamen University, China  

Copyright: © 2019 Bai, Zhang, Yang, Wu, Song, Li, Yan, Huang, Lin and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Yi Zhang, Third Xiangya Hospital, Central South University, Changsha, 410013, Hunan Province, China, yzhangxy3@126.com