AUTHOR=Xie Tianyu , Guo Xin , Wu Di , Li Shuo , Lu Yixun , Wang Xinxin , Chen Lin 

TITLE=PAFAH1B3 Expression Is Correlated With Gastric Cancer Cell Proliferation and Immune Infiltration

JOURNAL=Frontiers in Oncology

VOLUME=Volume 11 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.591545

DOI=10.3389/fonc.2021.591545

ISSN=2234-943X

ABSTRACT=Abstract
Background: Platelet activating factor acetylhydrolase 1b catalytic subunit 3 (PAFAH1B3) is associated with a variety of diseases. However, its function in gastric cancer remains uncertain.
Methods: PAFAH1B3 expression was analyzed in The Cancer Genome Atlas (TCGA) and genotype-tissue expression pan-cancer data. The effect of PAFAH1B3 on the clinical prognosis was evaluated using TCGA clinical survival data. Enrichment analysis of PAFAH1B3 was performed using the clusterProfiler R software package. We downloaded immune cell infiltration scores for TCGA samples examined in published articles and analyzed the correlation between PAFAH1B3 expression and immune cell infiltration. We also performed MTT and Celigo assays to assess the effect of PAFAH1B3 on cell proliferation.
Results: PAFAH1B3 was highly expressed in gastric cancer and some other tumors. High PAFAH1B3 expression was significantly correlated with proliferation-related gene sets involved in DNA replication, the cell cycle, and cell cycle checkpoints. Further analysis showed that M1 macrophage infiltration and CD8-positive T cells were increased in tissues with high PAFAH1B3 expression. PAFAH1B3 knockdown promoted apoptosis and inhibited the proliferation of gastric cancer cells.
Conclusions: Our findings suggest that PAFAH1B3 may be an oncogene in gastric cancer.