AUTHOR=Wang Chunhong , Su Haiyang , Cheng Rui , Ji Hongming 

TITLE=SPAG5 Is Involved in Human Gliomagenesis Through the Regulation of Cell Proliferation and Apoptosis

JOURNAL=Frontiers in Oncology

VOLUME=Volume 11 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.673780

DOI=10.3389/fonc.2021.673780

ISSN=2234-943X

ABSTRACT=Purpose: To explore the role of sperm-associated antigen 5 (SPAG5) in glioma. Methods: The association between SPAG5 expression and clinical features was initially investigated based on the Cancer Genome Atlas datasets (TCGA). The function of SPAG5 in glioma were analyzed using U87 and U251 cells. Knockdown glioma cells were constructed by shRNA interference. qRT-PCR and Western blotting were used to measure the expression of SPAG5 and CDH2. Cell proliferation and apoptosis were measured by MTT assay, Caspase 3/7 assay, and high-content screening (HCS) proliferation analysis and colony formation assay. Transwell assays and wound-healing assays were used to investigate cell migration and invasion. Results: The increased expression of SPAG5 was correlated with poor outcomes in glioma patients. Knocking down of SPAG5 could inhibit the proliferation and colony formation and promoted the apoptosis of glioma cells. Knocking-down of SPAG5 could also inhibit cell migration and invasion and the expression of CDH2. Over-expression of CDH2 with SPAG5 depletion could restored the proliferation and inhibited the apoptosis of glioma cells, which also promoted cell migration and invasion. Conclusions: SPAG5 is a promising prognostic factor and a potential therapeutic target for clinical intervention in glioma.