Choline metabolism and its implications in cancer

Choline, a quintessential quaternary ammonium compound, plays a cardinal role in several pivotal biological mechanisms, chiefly in safeguarding cell membrane integrity, orchestrating methylation reactions, and synthesizing vital neurotransmitters. This systematic review meticulously dissects the complex interplay between choline metabolism and its profound implications in oncology. The exposition is stratified into three salient dimensions: Initially, we delve into the intricacies of choline metabolism, accentuating its indispensability in cellular physiology, the enzymatic labyrinth governing its flux, and the pivotal cellular import mechanisms. Subsequently, we elucidate the contemporary comprehension of choline metabolism in the cancer paradigm, traversing its influence from inception to the intricate metamorphosis during oncogenic progression, further compounded by dysregulated enzyme activities and aberrant signaling cascades. Conclusively, we illuminate the burgeoning potential of choline-centric metabolic imaging modalities, notably magnetic resonance spectroscopy (MRS) and positron emission tomography (PET), as avant-garde tools for cancer diagnostics and therapeutic trajectory monitoring. Synoptically, the nuanced perturbations in choline metabolism in neoplastic entities unfurl critical insights, potentially heralding paradigm shifts in diagnostic and therapeutic oncological stratagems. A deeper foray into this realm is anticipated to fortify our molecular understanding and refine intervention modalities in cancer theranostics.


Introduction
Choline, a type of quaternary ammonium compound, is a vital nutrient involved in numerous biological functions, such as maintaining cell membrane integrity, facilitating methylation reactions, and aiding in the synthesis of neurotransmitters (1,2).It is a key component of phosphatidylcholine (PtdCho), a primary element of cell membranes, and plays a role in the creation of acetylcholine, a neurotransmitter that is crucial for nerve function (3).Choline can either be derived from the food we consume or be synthesized within our bodies (4).The liver is central to choline metabolism, where it is converted into phosphocholine and subsequently into PtdCho (5).Other organs, such as the brain, also have the capacity to produce choline, albeit to a lesser degree.Beyond its role in cellular structure and function, choline also contributes to methylation reactions.It provides methyl groups in the creation of S-adenosylmethionine, a universal methyl donor involved in the methylation of DNA, proteins, and lipids (6).This process is vital for the regulation of gene expression and protein function (6).Choline metabolism is interconnected with other metabolic pathways, including those of methionine and folate (7).Methionine serves as a precursor of Sadenosylmethionine, while folate is involved in the regeneration of methionine from homocysteine (8).The interaction between these pathways highlights the complexity of choline metabolism and its significance in cellular function.
Recent research has shown that choline metabolism undergoes significant changes in cancer, resulting in a heightened demand for choline and its metabolites (9).The rapid growth of cancer cells necessitates an increased production of PtdCho for the formation of new cell membranes (5,10).Furthermore, altered signaling pathways in cancer cells enhance the uptake and utilization of choline.These changes in choline metabolism have been linked to the onset of cancer, advancement of tumors, and resistance to treatment (11,12).Moreover, the use of choline-based metabolic imaging has shown great potential as a tool for both detecting and monitoring several types of cancer (13,14).
Choline metabolism shifts in cancer are not solely attributed to a heightened need for choline and its byproducts.These alterations also originate from modifications in the expression and functionality of enzymes involved in the metabolism of choline (8).In this systematic review, we aim to investigate the relationships between choline metabolism and its implications in cancer through the following three aspects: 1) choline metabolism; 2) current understanding of choline metabolism in cancer; and 3) use of choline-based metabolic imaging for cancer diagnosis and treatment monitoring.

Choline metabolism: an overview
Choline metabolism plays a central role in numerous cell functions, including the creation of cell membranes, single-carbon metabolism, and cholinergic neurotransmission (15).The metabolic pathway of choline is regulated by a series of enzymes, each contributing to the overall balance of choline and its derivatives within the cell (16).The first step in choline metabolism is its uptake into the cell, a process facilitated by choline transporters (17,18).These transporters are proteins that are embedded in the cell membrane and function to transport choline from the extracellular environment into the cell.This process is vital for maintaining the intracellular concentration of choline and ensuring that the cell has sufficient choline to meet its metabolic needs (17,18).When choline enters the cell, it is phosphorylated by choline kinase alpha (CHKa), resulting in the production of PtdCho (19).This stage is considered the pace-setting step in the production of PtdCho, a primary component of the cell membrane.PtdCho is vital for preserving the stability of the cell membrane and contributes to cell signaling and lipid transportation (20).
Phosphocholine cytidylyltransferase (CCT) then facilitates the conversion of PtdCho and cytidine triphosphate into cytidine 5'diphosphocholine (CDP-choline) (21,22).CDP-choline, the most activated choline intermediate in the Kennedy pathway, is directly utilized by diacylglycerol cholinephosphotransferase 1 to generate PtdCho (22).CCT exists in both a dormant soluble state and an active lipid-attached state within the nuclear membrane.Its activity is regulated by fluctuations in the intracellular concentration of choline and its byproducts.Besides these enzymes, choline m e t a b o l i s m i s a l s o a ff e c t e d b y o t h e r e n z y m e s l i k e phosphatidylethanolamine N-methyltransferase, which transforms phosphatidylethanolamine into PtdCho, thereby restoring cellular PtdCho levels (23).Other enzymes, including phospholipase A2, PtdCho-specific phospholipase D, and PtdCho-specific phospholipase C, participate in the breakdown of PtdCho, yielding free choline and other metabolites.These enzymes are vital in maintaining the equilibrium of choline and its metabolic products within the cell (23,24).

Choline metabolism in cancer initiation
Limited research has been conducted on the association between choline metabolism and cancer risk, with a focus primarily on dietary choline intake rather than plasma choline levels.In the available literature, plasma choline is mostly negatively correlated with new-onset cancer risk, including colorectal cancer (25,26), and pancreatic cancer (27), while one study indicates an increased risk of CRC associated with plasma choline levels (28).However, these studies are primarily nested case-control studies with their own inherent limitations, such as selection bias, and small sample size.

Choline metabolism in cancer progression
The dysregulation of choline metabolism plays a significant role in the initiation of cancer.This complexity arises from several factors, including the overexpression of enzymes involved in choline metabolism, changes in signaling pathways that promote choline uptake and utilization, and variations in choline transporters (Figure 1).
One key factor in choline dysregulation is the overexpression of enzymes involved in choline metabolism, such as CHKa.CHKa is crucial as it is essential for the synthesis of PtdCho, a primary CHKa, other enzymes involved in choline metabolism also exhibit disruptions in cancer.For example, glycerophosphodiester phosphodiesterase domain containing 5 (GDPD5) plays a role in regulating choline metabolism.Overexpression of GDPD5 has been linked to enhanced choline uptake and utilization, contributing to the dysregulation of choline metabolism in cancer (29).Furthermore, the onset of liver cancer has been associated with increased CCT activity and mRNA expression (21).Similarly, elevated CCT expression and activity have been observed in colon cancer, leading to higher PtdCho levels (22).
In cancer, choline metabolism becomes dysregulated not only due to enzyme overexpression but also because of changes in signaling pathways that enhance choline uptake and utilization (5,30).The MAPK pathway, which is a central regulator of various cellular processes, is altered in cancer, leading to increased choline uptake and use.The activation of CHKa and elevated PtdCho levels were first observed in NIH3T3 fibroblasts stimulated with serum and transformed with either KRAS or HRAS.The activation of the MAPK pathway by oncogenic HRAS results in the regulation of CCT transcription (11).Another study integrated transcriptome and metabolome data and found that dehydrogenase/reductase member 2 (DHRS2) disrupts choline metabolism in ovarian cancer (31).Specifically, DHRS2 post-transcriptionally downregulates CHKa, inhibiting AKT signaling activation, which in turn leads to a reduced PtdCho/glycerophosphorylcholine ratio.This disruption in choline metabolism reprogramming is a primary factor behind DHRS2's tumor-suppressive effect in ovarian cancer.
Choline transporters are another crucial component of choline metabolism in cancer (32).These transporters are in charge of absorbing free choline from the surroundings, a process that can be a pace-setting stage in the creation of PtdCho and within the Kennedy pathway (32).The human genome contains four sets of proteins capable of transporting choline: organic cation/carnitine transporters, choline transporter 1 (CHT1/SLC5A7), organic cation transporters (OCTs) and choline transporter-like proteins (10, 33).In order to secure the uptake of this essential nutrient, cancer cells typically express at least one category of choline transporters (34,35).The expression levels of choline transporter genes and the speed of choline absorption in cancer cells are frequently much greater than in non-cancerous cells (35).For instance, breast cancer cell lines have exhibited elevated expression of CHT1 and OCT2 when compared to non-cancerous human mammary epithelial cells (33).The heightened requirement for choline in cancer cells implies their potential susceptibility to inhibition of choline transporters.Organic cation drugs, which probably use the same transporters as choline, could hinder choline absorption, thus decreasing the viability of cancer cells.For example, the choline analogue hemicholinium-3 and tetrahexylammonium chloride have been documented to hinder choline absorption and decrease cell growth in specific cancer cell lines (35).

Therapeutic strategies targeting choline metabolism
Choline metabolism is emerging as a pivotal focus in cancer research, offering promising therapeutic avenues.The inhibition of oncogenic signaling pathways with specific anticancer drugs induces changes in choline-containing metabolite levels, underscoring its therapeutic potential (32).Delving deeper, aberrations in choline metabolism in cancers have been identified, paving the way for innovative treatments targeting this metabolic pathway (36).The role of choline phospholipid metabolism is accentuated when considering its molecular targets, which present opportunities for groundbreaking anticancer therapies (37).Furthermore, the potential of this metabolic Choline metabolism in cancer progression.
pathway is magnified when enzymes, such as choline kinase, are targeted, heralding a new in anticancer treatment strategies (38).
In conclusion, the disruption of choline metabolism in cancer is a complex process involving multiple factors.Gaining a comprehensive understanding of this process can yield valuable insights into the underlying biology of cancer and potentially pave the way for novel approaches in cancer diagnosis and treatment (35).

Choline-based metabolic imaging for cancer diagnosis and treatment monitoring
Choline-based metabolic imaging has emerged as a promising tool for the detection and monitoring of cancer, offering potential applications in cancer diagnosis and treatment assessment (39).This approach leverages the alterations in choline metabolism that occur in cancerous cells, providing a unique avenue for noninvasive cancer detection and monitoring (35,40).As mentioned previously, cancer cells often exhibit dysregulated choline metabolism, leading to an increased demand for choline and its metabolites.Metabolic imaging techniques based on choline, such as magnetic resonance spectroscopy (MRS) and positron emission tomography (PET) and fluorescence imaging, have been developed to exploit these metabolic alterations for cancer diagnosis and treatment monitoring (41)(42)(43).These techniques allow for noninvasive visualization and quantification of choline and its metabolites in tumors, providing valuable information about tumor biology and response to therapy (42).PET imaging using radio-tagged choline has proven to be successful in identifying a range of cancers, such as liver and prostate cancer (44,45).This technique provides high-resolution images of choline uptake in tumors, allowing for accurate localization and staging of the disease.Moreover, choline PET imaging can be employed to track the reaction to treatment, as changes in choline uptake can reflect alterations in tumor metabolism and growth (46).Similarly, MRS can be used to measure the levels of choline and its metabolites in tumors (42).This technique provides a metabolic profile of the tumor, which can provide insights into tumor biology and response to therapy (42).For instance, an increase in the choline peak in MRS spectra has been linked to tumor advancement, while a decrease in the choline peak has been associated with response to therapy (47).Also, fluorescence imaging is a very convenient method to monitor CHKa in vivo (48).Table 1 highlights the most relevant studies Review NA The elevation of the choline peak in magnetic resonance spectroscopy spectra is linked to tumor progression, whereas a reduction in the choline peak is connected to a positive response to therapy.
Arlauckas SP, 2017 Experimental study NA A ChoKa inhibitor called JAS239 could be used as a new method to monitor effective inhibitors of choline metabolism in breast cancer, which distinguishes tumors and delineates tumor margins in vivo.
focused on choline-based metabolic imaging for cancer diagnosis and treatment monitoring.
In conclusion, choline-based metabolic holds promise as a valuable tool for detecting and monitoring cancer, offering potential applications in both cancer diagnosis and treatment evaluation.By leveraging the alterations in choline metabolism that occur in cancer, these techniques provide a unique avenue for non-invasive cancer detection and monitoring.However, further research is needed to optimize these techniques and to fully elucidate the implications of choline metabolism in cancer.

Conclusions
The intricate interplay between choline metabolism and cancer opens up new avenues for advancements in cancer detection and therapeutic interventions.The disruption of choline metabolism in cancer cells has been linked to the onset of cancer, advancement of tumors, and resistance to therapy, implying that choline metabolism could serve as a viable target for both cancer detection and therapeutic interventions.The potential of choline-based metabolic imaging for cancer diagnosis and treatment monitoring is currently being explored, with promising results.However, additional research is required to completely clarify the mechanisms behind the changes in choline metabolism in cancer and to develop effective cholinebased diagnostic and therapeutic strategies.The complexity of choline metabolism and its importance in various biological processes underscore the need for a multifaceted approach to cancer research.By integrating insights from different fields, including biochemistry, molecular biology, and imaging science, we can gain a more comprehensive understanding of the role of choline metabolism in cancer and develop more effective strategies for cancer diagnosis and treatment.

TABLE 1
The relevant studies linking the choline-based metabolic imaging for cancer diagnosis and treatment monitoring.newly developed carbocyanine dye, can inhibit the activity of choline kinase alpha (ChoK) in breast cancer cells, leading to cell death, and it has the potential to be used as a companion diagnostic tool for noninvasive breast tumor staging and as a novel treatment for aggressive, therapy-resistant tumors.