Identification of both GABAA receptors and voltage-activated Na+ channels as molecular targets of anticonvulsant α-asarone

Alpha (α)-asarone, a major effective component isolated from the Chinese medicinal herb Acorus tatarinowii, is clinically used as medication for treating epilepsy, cough, bronchitis, and asthma. In the present study, we demonstrated that α-asarone targets central nervous system GABAA receptor as well as voltage-gated Na+ channels. Using whole-cell patch-clamp recording, α-asarone inhibited spontaneous firing of output neurons, mitral cells (MCs), in mouse olfactory bulb brain slice preparation and hyperpolarized the membrane potential of MCs. The inhibitory effect of α-asarone persisted in the presence of ionotropic glutamate receptor blockers but was eliminated after adding a GABAA receptor blocker, suggesting that GABAA receptors mediated the inhibition of MCs by α-asarone. This hypothesis was supported by the finding that α-asarone evoked an outward current, but did not influence inhibitory postsynaptic currents (IPSCs). In addition to inhibiting spontaneous firing, α-asarone also inhibited the Nav1.2 channel, a dominant rat brain Na+ channel subtype. The effects of α-asarone on a defined Nav1.2 were characterized using transfected cells that stably expressed the Nav1.2 channel isoform. α-Asarone displayed strong tonic inhibition of Nav1.2 currents in a concentration- and membrane potential-dependent fashion. α-Asarone reduced channel availability in steady-state inactivation protocols by enhancing or stabilizing Na+ channel inactivation. Both Na+ channel blockade and activation of GABAA receptors provide a possible mechanism for the known anti-epileptic effects of α-asarone. It also suggests that α-asarone could benefit patients with cough possibly through inhibiting a Na+ channel subtype to inhibit peripheral and/or central sensitization of cough reflexes.

Several bioactivities of α-asarone have been reported recently, e.g., reducing the level of lipids (Cassani-Galindo et al., 2005), a neuroprotective effect (Limón et al., 2009) and reducing excitatory synaptic activity (Gu et al., 2010). In China, α-asarone is approved for the treatment of epilepsy, bronchitis, cough, and asthma, indicating that it is effective in treating two disorders, namely respiratory diseases and neuronal disorders in clinical practice. The beneficial effects of α-asarone on these two unrelated disorders could suggest that α-asarone binds to more than one protein target. The possible protein targets that α-asarone binds to and the molecular mechanism(s) underlying the distinct actions of α-asarone remain to be determined. It has been reported that α-asarone prolonged the falling down latent period of induced asthma and relaxed smooth muscle of trachea by antagonizing histamine and acetylcholine. Recently, it was reported that α-asarone alleviates epilepsy by modulating GABA A receptors (Huang et al., 2013). Although potential mechanisms underlying the anti-epileptic effect and the beneficial effect for respiratory diseases have been studied, the pharmacological bases of these actions need to be further elucidated. Here, we hypothesize that α-asarone binds to distinct disease-specific protein targets to exert effects on both respiratory and neuronal disorders, respectively. Alternatively, it binds to distinct protein targets of one disorder and, thereby, displays synergistic effects for that disorder.
Cough is a prominent symptom of respiratory disorders such as chronic obstructive pulmonary disease, lung cancer, bronchitis, and asthma. Persistent cough may be due to peripheral and/or central sensitization of cough reflexes (Chung, 2002;Undem and Carr, 2010). Primary afferent nerves are regarded as novel target for antitussive therapy (Undem and Carr, 2010;Muroi et al., 2013;Spina and Page, 2013). Sodium channels play an important role in the cough reflexes. This has been shown by the fact that local anesthetics, blockers of sodium-dependent channels, are inhibitors of the cough reflex (Chung, 2002;Spina and Page, 2013). Recently, in an effort to functionally screen the bioactivity of a small group of natural products, we discovered that one of them, methyleugenol, emerged as a novel sodium (Na + ) channel blocker (Wang et al., unpublished data). α-Asarone is a compound which is similar to methyleugenol in its chemical structure (Figure 1). Thus, we hypothesize that α-asarone may display similar functional properties such as Na + channel blockade to inhibit the cough reflex.
α-Asarone has recently been shown to inhibit synaptically driven-spiking of hippocampal neurons to exert an anti-epileptic effect through activating GABA A receptors (Huang et al., 2013). It is well known that many bioactive agents interact with more than one therapeutic target protein. For example, several anticonvulsant agents such as valproic acid, gabapentin, and topiramate have been shown to inhibit the function of Na + channels and, at the same time, activate GABA A receptors (Rho and Sankar, 1999). Felbamate and phenobarbital are anticonvulsant agents that interact with both N -methyl-D-aspartate (NMDA) and GABA A receptors (Rho et al., 1994;McNamara, 1996). The pharmacological properties of these anticonvulsant agents suggest that an ideal anticonvulsant agent could target two or more epilepsy-related proteins or ion channels to control neural hyperexcitability. Thus, α-asarone may possibly interact with more than one therapeutic protein to exert its anti-epileptic effects. In central nervous system (CNS), voltage-gated type II/IIA Na + channels (Na v 1.2A) are preferentially localized in axons (Westenbroek et al., 1989). The tetrodotoxin (TTX)-sensitive channel isoform Na v 1.2A has been studied as the target of multiple anti-epileptics and the inhibition of Na v 1.2A is considered as an anticonvulsant mechanism (Ragsdale et al., 1991;Dupere et al., 1999). Therefore, based on the chemical similarity of α-asarone to methyleugenol, Na v 1.2 is a good candidate to explore its possible regulation by α-asarone.
Epileptic seizures result from poorly controlled neuronal activity at a seizure focus and the subsequent spread of electrical excitation in brain circuits (Rall and Schleifer, 1990). It is not surprising that most effective anti-seizure medications inhibit neuronal excitability by modulating the function of several types of proteins such as Na + channels, NMDA receptors, and GABA receptors (Rho and Sankar, 1999). Output neurons such as mitral cells (MCs) in the mouse main olfactory bulb (MOB) display their neuronal activity as spontaneous action potential firing, which can be modulated by intrinsic membrane receptors as well as synaptic inputs (Shepherd et al., 2004;Ennis et al., 2007;Wang et al., 2011). In the rodent MOB, MCs express high levels of different receptors such as GABA receptors (GABA A , GABA B ), ionotropic and metabotropic glutamate receptors (NMDA,AMPA, metabotropic glutamate receptor 1 (mGluR1), kainite). Most of these receptor proteins are thought to be epilepsy-related (McNamara, 1996;Snell et al., 2000;Wang et al., 2002;Ennis et al., 2007). Therefore, compared to the synaptically driven-firing neurons (Huang et al., 2013), spontaneous firing MCs is a better choice for exploring the possible regulation of neuronal excitement by α-asarone. In this study, we used acute slices of the mouse MOB as well as stably transfected cells expressing the Na v 1.2 channel α-subunit to determine the potential cellular targets of α-asarone.

CELL CULTURE
The CNaIIA cell line (gift from Dr. W.A. Catterall) was derived from a Chinese hamster ovary (CHO-K1) cell line stably transfected with a cDNA encoding the rat brain type IIA Na + channel (Na v 1.2A; Ragsdale et al., 1991;Scheuer et al., 1992). CNaIIA cells were cultured in Roswell Park Memorial Institute medium (Gibco) with 5% fetal bovine serum, and 100 μg/ml streptomycin and 100 U/ml penicillin. G418 (400 μg/ml) was included to select for transfectants. Then the cells were passed and plated on glass coverslips in 35-mm dishes in a 5% CO 2 atmosphere at 37 • C for 1-3 days before experimentation.

WHOLE-CELL VOLTAGE-CLAMP RECORDING FROM TRANSFECTED CHO CELLS
Na + currents were recorded using the whole-cell patch clamp recording technique (Hamill et al., 1981). The cultured cells on coverslips were transferred to a handmade recording chamber and continuously perfused at room temperature with extracellular solution containing (in mM): 130 NaCl, 4 KCl, 1.5 CaCl 2 , 1.5 MgCl 2 , 5 glucose, 5 HEPES, 20 sucrose, pH 7.4 adjusted with NaOH. The recording chamber volume was approximately 0.4 ml and the flow rate was 0.6 ml/min. MP-285 micromanipulator (Sutter Instrument Co., Novato, CA, USA) was used to place the electrode onto the cell. Patch pipettes were pulled from borosilicate glass capillaries (Drummond Scientific Co., Broomall, PA, USA) on an electrode puller (Model P-97, Sutter Instrument Co.) and were filled with a 0.2 μm filtered internal solution containing (in mM): 90 CsF, 60 CsCl, 10 NaCl, 5 HEPES, pH 7.4 adjusted with NaOH. The pipettes had input resistance of 0.8-1.4 M . Recordings were performed at room temperature (22 • C) with a patch clamp EPC 9 (HEKA Elektronik GmbH, Germany) and were filtered at 5 kHz. Leakage currents were subtracted using a P/4 or P/2 protocol. Pulse (HEKA Elektronik) was used for experimental control and basic data analysis.
The methods of recording Na + currents from Na v 1.2 expressing cells were similar to our previous report (Wang et al., 2002). Cells with series resistance of less than 2.5 M were used for drug test experiments. Only cells with whole cell maximal Na + currents of at least 1 nA were used in the analysis. Na + currents recorded from Na v 1.2A expressing cells always increased progressively within the first 20 min after establishing the whole-cell-recording configuration and then were relatively stable. Thus, drugs were applied only during this period (after 20 min), especially for testing tonic inhibition evoked by the drug. Time-dependent shifts (∼0.5 mV before and after perfusion) in the inactivation curve of Na v 1.2 have not been considered in calculating the shift by drug.

SLICE PREPARATION
Wild type mice (C57BL/6J, Jackson Laboratory, Bar Harbor, ME, USA) were used in agreement with Institutional Animal Care and Use Committee and NIH guidelines. Juvenile (16-to 25-day old) mice were decapitated, and the MOBs were dissected out and immersed in artificial cerebrospinal fluid (ACSF, see below) at 4 • C, as previously described (Heinbockel et al., 2004). Horizontal slices (400 μm-thick) were cut parallel to the long axis using a vibratome (Vibratome Series 1000, Ted Pella Inc., Redding, CA, USA). After 30 min at 30 • C, slices were incubated in a holding bath at room temperature (22 • C) until use. For recording, a brain slice was placed in a recording chamber mounted on a microscope stage and maintained at 30 ± 0.5 • C by superfusion with oxygenated ACSF flowing at 2.5-3 ml/min.

SLICE RECORDING AND DATA ACQUISITION
Visually guided recordings were obtained from cells in the MC layer with near-infrared differential interference contrast optics and a BX51WI microscope (Olympus Optical, Tokyo, Japan) equipped with a camera (C2400-07, Hamamatsu Photonics, Japan). Images were displayed on a Sony trinitron color video monitor (PVM-1353MD, Sony Corp. Japan). Recording pipettes (5-8 M ) were pulled on a Flaming-Brown P-97 puller (Sutter Instrument Co.) from 1.5 mm O.D. borosilicate glass with filament. Seal resistance was routinely >1 G and liquid junction potential was 9-10 mV; reported measurements were not corrected for this potential. Data were obtained using a Multiclamp 700B amplifier (Molecular Devices, Sunnyvale, CA, USA). Signals were low-pass Bessel filtered at 2 kHz and digitized on computer disk (Clampex 10.1, Molecular Devices). Data were also collected through a Digidata 1440A Interface (Molecular Devices) and digitized at 10 kHz. Holding currents were generated under control of the Multiclamp 700B Commander.

CHEMICALS AND DRUG APPLICATION
For all experiments, drugs were bath perfused at the final concentrations indicated by dissolving aliquots of stock solution in ACSF or in extracellular solution. α-Asarone was supplied by the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Stock solution of 40 mM α-asarone was prepared in dimethyl sulfoxide (DMSO) and then diluted to the desired concentrations for experiments (final concentration of DMSO in bath < 0.1%). The following drugs were also bath applied: L-2-amino-5-phosphonopentanoic acid (AP5, APV), 6-cyano-7-nitroquinoxaline-2-3-dione (CNQX), 2-(3-carboxypropyl)-3-amino-6-(4 methoxyphenyl)-pyridazinium bromide (gabazine, SR-95531). Chemicals were supplied by Tocris (Ellisville, MO). Control recordings showed that 0.1% DMSO had no detectable effects on MC activity and the Na + currents in transfected CHO cells.

DATA ANALYSIS
The data obtained with cultured CHO cells were analyzed using a combination of PulseFit (HEKA Elektronik) and SigmaPlot 9.0 (Jandel Scientific, Corte Madera, CA, USA) software. The data obtained with slice recordings were analyzed using a combination of Clampfit 10.2 (Molecular Devices) and Origin8 (OriginLab Corporation, Northampton, MA, USA). All results are presented as the mean SEM. Tests for statistical significance were performed using paired Student's t-tests, and one-way ANOVA followed by the Bonferroni test for multiple comparisons.

RESULTS
In culture preparations, 20-30% of Na v 1.2 cells displayed fast, transient inward currents following depolarization. The maximal peak inward currents of Na v 1.2 ranged from 0.7 to 8 nA. However, in a few cells, it went up to 20 nA. These currents were blocked completely by 0.5 μM TTX, confirming their identity as uncontaminated Na + currents under these recording conditions. In slices, MCs were identified visually by their soma location and relatively large soma size, and by their input resistance www.frontiersin.org (297 ± 19.2 M , n = 46). Spontaneous firing is an intrinsic property of MCs (Ennis et al., 2007).

α-ASARONE TONICALLY INHIBITED RAT BRAIN Na v 1.2 CURRENTS
A specific Na + channel subtype (Na v 1.2) is known to be dominant in the rat brain (Westenbroek et al., 1989). To determine whether α-asarone directly interacted with central nervous Na + channels, we subjected the drug to procedures similar to those used for phenytoin and carbamazepine (CBZ) characterization (Ragsdale et al., 1991).
Bath application of α-asarone reversibly reduced the amplitude of the Na v 1.2 peak currents (Figure 2). The currents of Na v 1.2 shown in Figure 2A were elicited by stepping to various depolarized potentials from a holding potential of −100 mV. Figure 2B shows the current-voltage (I-V) relationship of Na v 1.2 channels in control and in various concentrations of α-asarone, indicating that α-asarone inhibited Na + currents. The shape of the I-V curve was unaffected by concentrations of α-asarone, suggesting that α-asarone, at the concentrations tested, had no effect on the voltage-dependent activation of Na + channels, which was further analyzed in Figure 3. In Figure 2C, the currents were elicited by stepping to 0 mV from a holding potential of −100 mV, demonstrating that the tonic inhibition of Na v 1.2 channels by αasarone was concentration-dependent. Figure 2D shows the tonic inhibition by α-asarone on Na v 1.2 currents which was determined by measuring concentration-response relationships at −100, and −60 mV holding potentials, respectively. At −100 mV, almost all channels were in the resting state which was observed by measuring the inactivation curve of Na v 1.2 (shown in Figure 3). At −60 mV holding potential, about 15% of the channels were in the inactivated state. The averaged inhibition evoked by α-asarone at varying concentrations was well fit to the Hill equation. Based on The I-V relationships in control and in the presence of various concentrations of asarone. The currents were elicited by stepping to various depolarized potentials (ranging from -80 to +100 mV in 10-mV increments) for 9 ms, and then returning to the holding potential of -100 mV. Peak currents at each depolarized potential were measured. The data are from a representative cell. (C) Superimposed current traces recorded before (control), during the application of 20 μM, 500 μM, and 2 mM α-asarone. The currents were recorded from the same cell and were elicited by a 10-ms pulse to 0 mV from the −100 mV holding potential. (D) Concentrationresponse curves for the inhibition of Na + currents by α-asarone at different holding potentials. The cells were held at -100 mV, and -60 mV, respectively, and stepped to 0 mV for 10 ms. The peak currents in the presence of asarone were normalized to the corresponding control peak currents, and then averaged. Each point was the mean ± SEM of 4-6 cells. The lines are best fits for data to the Hill equation: y = 1 − x n /(K n d + x n ), where y is the fractional current, K d is the apparent dissociation constant for asarone, and n is the Hill coefficient. K d and n were estimated using a Marquadt non-linear least-squares routine.

FIGURE 3 | Effects of α-asarone on voltage-dependent availability and activation of Na v 1.2 channels.
The shift of inactivation curves of Na v 1.2 channels by 500 μM asarone (n = 5). The voltage dependence of steady-state inactivation (h ∞ ) was examined by applying 500-ms prepulse potentials from -120 to 10 mV in 10-mV increments from a holding potential of -100 mV before stepping to the test potential (0 mV) for 35 ms. The peak current (I) for each cell was normalized with respect to the first value measured at test potential (0 mV). Conductance-voltage relationship: from the peak Na + currents obtained in Figures 2A,B, the Na + conductance values (G) in the absence and presence of 500 μM α-asarone were calculated, normalized to the maximum in control, and plotted as a function of membrane potentials (V ). The smooth curves through the data are drawn according to the equation: Where V = membrane potential, V h = the prepulse potential where the current is half-maximal, and k = the slope factor. a fitted Hill coefficient (n) value of 1.2 at a holding potential of −60 mV, it appeared that the stoichiometry of drug and receptor interaction was 1:1. The estimation of IC 50 value obtained by fit to the Hill equation was 913 μM at −100 mV, and 217 μM at −60 mV, respectively. The result indicates that α-asarone exhibited a much higher affinity for channels at the −60 mV holding potential, where a subset of channels was in the inactivated state.

α-ASARONE SHIFTED THE STEADY-STATE INACTIVATION CURVE
Na + channel blockers like CBZ and phenytoin have voltagedependent effects on the availability of Na + currents, as seen by a shift of the steady-state inactivation curve to more negative potentials (Schwartz et al., 1989;Ragsdale et al., 1991). The steady-state inactivation (h ∞ ) curve was examined to elucidate the effects of α-asarone on the voltage-dependent availability of Na v 1.2 channels.
α-Asarone displayed voltage-dependent effects on the availability of Na v 1.2 current. It shifted inactivation curves toward more negative potentials (Figure 3). The currents for each cell were normalized and averaged, and then fit to a single Boltzmann relationship from which the mean V h and k values were calculated. The slope factors (k) of the curves were not affected at the concentrations of α-asarone we tested. The results suggest that α-asarone might interact preferentially with the inactivated state of the channels, which is typically found at depolarized potentials.

α-ASARONE SUPPRESSED SPONTANEOUS SPIKING OF MITRAL CELLS AND HYPERPOLARIZED THEIR MEMBRANE POTENTIAL
The above results indicate that α-asarone acts on Na + channels. In addition to Na + channels, α-asarone may interact with other target proteins that are of clinical importance. MCs are output neurons in the MOB and spontaneously generate action potentials which can be modulated by intrinsic membrane properties as well as synaptic inputs (Shepherd et al., 2004;Ennis et al., 2007;Wang et al., 2011). We used the intrinsic properties of MCs such as spontaneous firing, membrane potential, and membrane conductance to evaluate the effect of α-asarone on neuronal activity and to determine the possible molecular mechanisms underlying the effects.
Bath application of α-asarone modulated the spiking rate of MCs (Figure 4). Compared to control conditions, 20 μM α-asarone significantly reduced the MC firing rate. α-Asarone reversibly decreased MC firing rate from 4.7 ± 0.5 Hz to 2.9 ± 0.5 Hz (n = 9; p < 0.0001, paired t-test). The reduction of firing rate was accompanied by a hyperpolarization of the MC membrane potential from −50.8 ± 1.2 to −52.3 ± 1.2 mV (n = 8; p < 0.001, paired t-test). Figure 4 illustrates the inhibitory effect of α-asarone in an original recording from a typical MC.

GABA A RECEPTORS RATHER THAN IONOTROPIC GLUTAMATE RECEPTORS WERE INVOLVED IN THE α-ASARONE-INDUCED INHIBITION OF NEURONAL EXCITABILITY
Ionotropic glutamate receptors play a critical role in the regulation of neuronal excitability in the MOB (Ennis et al., 2007). Blockade of ionotropic glutamate receptors can result in neuronal inhibition. We examined the effects of α-asarone-induced neuronal inhibition in the presence of AMPA/kainate and NMDA receptor blockers.
To further examine the role of GABA A receptors in α-asaroneevoked inhibition, the effect of α-asarone was measured during GABA A receptor blockade. Blockade of GABA A receptors can result in excitation or disinhibition of neurons. In the presence of gabazine, the inhibition of MC activity by α-asarone was eliminated (in gabazine: 5.3 ± 1.0 Hz; in gabazine plus α-asarone: 5.2 ± 1.0 Hz; n = 6, p > 0.05, paired t-test; Figure 5C). These results indicate that GABA A receptors rather than ionotropic glutamate receptors were involved in the α-asarone-evoked inhibition of MC activity.
Further evidence for the involvement of GABA A receptors came from measurements of MC ionic currents induced by αasarone. In whole-cell voltage clamp recording mode with Na + channel blocker QX-314 included in the pipette solution, 20 μM α-asarone evoked an outward current in MCs of 11.8 ± 2.1 pA (n = 10; range 2.0-17 pA). The steady-state currents at the holding potential in α-asarone were measured and subtracted from that in ACSF (Figure 6). In the presence of gabazine, α-asarone-induced outward currents were blocked (−0.3 ± 1.5 pA, n = 4; the currents in α-asarone plus gabazine was subtracted from that in gabazine).
The gabazine-mediated blockade of α-asarone-evoked inhibition of MCs suggests that α-asarone either binds to GABA A receptors to generate the inhibitory effects or it enhances extracellular GABA levels. α-Asarone-evoked outward currents may result from an enhancement of GABA release. This hypothesis was not supported by our observation that α-asarone was not able to increase the frequency of spontaneous inhibitory postsynaptic currents (sIPSCs) in MCs (Figure 6), suggesting that α-asarone enhanced tonic GABAergic inhibition rather than phasic GABAergic inhibition. With the recording conditions of Figure 6A, sIPSCs were upward and exhibited some rundown. Thus, a high-Cl − pipette solution was used for sIPSCs recordings in subsequent experiments. In conditions of a high-Cl − pipette solution (containing QX-314), the sIPSCs were downward deflected. α-Asarone induced an outward current as seen in Figure 6B, but did not change the frequency of sIPSCs (in control: 2.3 ± 0.3 Hz; in α-asarone: 2.4 ± 0.4 Hz) (n = 7; p > 0.05, paired t-test). The sIPSCs were completely eliminated by gabazine, indicating that sIPSCs were mediated by GABA acting on GABA A receptors. In the presence of Na + channel blocker (QX-314), α-asarone induced outward currents in MCs, but did not significantly alter sIPSC frequency, suggesting that asaroneevoked inhibition is mediated by direct action on GABA A receptors rather than by indirect action through the elevation of tissue GABA levels.

DISCUSSION
In the present study, we showed that α-asarone has a dual effect on neuronal proteins. It inhibited spontaneous activity of output neurons through activation of GABA A receptors in the MOB and it inhibited voltage-gated Na + currents of the rat brain Na + channel α-subtype (Na v 1.2). Our study shows that α-asarone markedly inhibited Na + currents of Na v 1.2, which are known to be dominant in the rat brain, in a concentration and membrane potential-dependent manner. α-Asarone reduced the availability of Na + channels in steady-state inactivation protocols by enhancing or stabilizing Na + channel inactivation. In addition to the blockade of Na + channels, α-asarone inhibited neuronal activity of MCs. Ionotropic glutamate receptors were not involved in α-asarone-evoked inhibition of neuronal activity. Instead, α-asarone-evoked neuronal inhibition was mediated by direct activation of GABA A receptors in the MOB rather than by increasing GABA release from GABAergic interneurons, suggesting the nonsynaptic GABA A receptors might be responsible for the effect of α-asarone inhibition. Therefore, we identified both GABA A receptors and voltage-activated Na + channels as cellular targets of α-asarone.
α-Asarone inhibits synaptically driven spiking in hippocampal neurons (Huang et al., 2013). Spiking in these hippocampal neurons is driven by glutamate and can be blocked by an AMPA receptor antagonist (Huang et al., 2013). The principal neurons in MOB are anatomically and functionally different from hippocampal neurons. MCs in the MOB are output neurons and exhibit their neuronal activity as spontaneous action potential firing, which can be modulated through intrinsic membrane properties as well as synaptic inputs (Shepherd et al., 2004;Ennis et al., 2007). Here, we found that α-asarone inhibited spontaneous, intrinsic spiking of MCs through activation of GABA A receptors. Thus, α-asarone can inhibit both synaptically driven spiking and intrinsic spiking in mouse neurons, possibly to exert the anti-epileptic effects.
α-Asarone interacted directly with Na + channels. Since Na + channel blockers are often able to suppress sustained repetitive firing of neurons as use-dependent inhibition, we tested the effect of α-asarone on sustained repetitive firing of MCs in the presence of 5 μM gabazine (gabazine was used to block the action of α-asarone on GABA A receptors). By injecting 100, 200 and 400 pA currents (500 ms) to depolarize MCs, the frequency of firing during the current injection was measured in the presence and absence of α-asarone. Unexpectedly, we did not see a significant reduction of firing rate by α-asarone (data not shown; only two out of 14 cells displayed the inhibition by 20 μM and 40 μM α-asarone on sustained repetitive firing). We are not sure why α-asarone did not show blockade of action potential in our recording condition. It is possible that the concentrations of α-asarone we used are not high enough to block firing. The failure of blocking firing at tested concentrations implies that Na channels may not contribute the a-asrarone-induced inhibition at the concentration of 20 and 40 μM. However, we do find use-dependent inhibition by 40 μM α-asarone in expressed CHO cell (data is not shown). Since α-asarone is a Na + channel blocker, how can it fail to suppress sustained firing? Several scenarios might explain the lack of α-asarone effect on sustained firing. Firstly, increased input resistance (R i ) are observed with GABA A blockers (data not shown). The increased R i by GABA A blockers might partly explain the lack of effect of α-asarone on firing rate. Secondly, it is well known that a substantial proportion of patients with chronic epilepsy (up to 30%) remain refractory to antiepileptic drugs, i.e., not all neuronal activity at a seizure focus and the subsequent spread of electrical excitation in brain circuits can be well controlled by antiepileptic drugs including Na + channels blockers. Possibly, Na + channels blockers such as anticonvulsant CBZ may not be able to suppress epileptic-like activity in some specific area in the brain such as MOB. Thirdly, it has been reported that the action of the CBZ on Na + channel appears to depend on the presence of the accessory β1 Na + channel subunit (Kazen-Gillespie et al., 2000). The involvement of the β1 subunit in drug-Na + channel interactions was shown by the fact that functional loss of accessory Na + channel subunits is a feature of a number of neurological disorders, including epilepsy (Gastaldi et al., 1998;Ellerkmann et al., 2003;Patino et al., 2009;Uebachs et al., 2012). Na + channel αand β-subunit mRNAs exhibit differential expression in the CNS, suggesting that the subtypes have distinct physiological roles and contribute to the functional heterogeneity of neurons in the CNS (Whitaker et al., 2000(Whitaker et al., , 2001. A localization study of Na + channel β1 and β2 mRNA in the CNS demonstrates a differential expression in different neurons (Oh et al., 1994;Levy-Mozziconacci et al., 1998). It will be interesting to determine if accessory β1 or β2 subunits are functionally expressed in MCs. Fourthly, Na + channel blockers such as eugenol have been www.frontiersin.org reported to inhibit Na + currents that are not dependent on the stimulus frequency in rat dorsal root ganglion neurons (Cho et al., 2008). It remains to be tested if commonly used Na + channel blockers such as CBZ and phenytoin can effectively suppress sustained firing of MCs in the MOB. In the meantime, there is no report showing that anticonvulsants such as CBZ cause partial loss of olfaction. Since MCs are principal neurons in the MOB, the reduction/blockade of spontaneous firing of MCs may alter odor processing.
Persistent cough may be due to peripheral and/or central sensitization of cough reflexes (Chung, 2005;Undem and Carr, 2010). The primary afferent nerves are regarded as novel target for antitussive therapy (Undem and Carr, 2010;Muroi et al., 2013;Spina and Page, 2013). Lidocaine, a local anesthetic that acts by blocking Na + channels, is an inhibitor of the cough reflex (Chung, 2002;Spina and Page, 2013). It is possible that α-asarone may exert its antitussive effect as a Na + channel blocker to inhibit cough reflexes. Thus, our findings provide a possible mechanism underlying the effects of α-asarone on cough which is a prominent symptom of bronchitis, asthma, chronic obstructive pulmonary disease, and lung cancer. We identified both GABA A receptors and voltage-activated Na + channels as cellular targets of α-asarone. These cellular targets might be of therapeutic relevance in the treatment of both epilepsy and respiratory diseases. The precise mechanism by which α-asarone inhibits cough reflexes in animal models or humans remains to be elucidated.

AUTHOR CONTRIBUTIONS
Ze-Jun Wang conducted the experiments, analyzed the data, provided intellectual input, and wrote the manuscript. Simon R. Levinson provided intellectual input. Liqin Sun conducted the experiments. Thomas Heinbockel provided intellectual input and wrote the manuscript.

ACKNOWLEDGMENTS
We are grateful to Dr. William A. Catterall, Department of Pharmacology University of Washington, for his kind gift of CNaIIA cells. This work was supported in part by U.S. PHS grants (GM08016, MD07597) to Thomas Heinbockel and the Foundation of Selected Item Support for Researcher Back from Overseas to Ze-Jun Wang (sponsored by Ministry of Personnel, P.R.C.).