Ion Channels in Obesity: Pathophysiology and Potential Therapeutic Targets

Obesity is a multifactorial disease related to metabolic disorders and associated with genetic determinants. Currently, ion channels activity has been linked to many of these disorders, in addition to the central regulation of food intake, energetic balance, hormone release and response, as well as the adipocyte cell proliferation. Therefore, the objective of this work is to review the current knowledge about the influence of ion channels in obesity development. This review used different sources of literature (Google Scholar, PubMed, Scopus, and Web of Science) to assess the role of ion channels in the pathophysiology of obesity. Ion channels present diverse key functions, such as the maintenance of physiological homeostasis and cell proliferation. Cell biology and pharmacological experimental evidences demonstrate that proliferating cells exhibit ion channel expression, conductance, and electrical properties different from the resting cells. Thereby, a large variety of ion channels has been identified in the pathogenesis of obesity such as potassium, sodium, calcium and chloride channels, nicotinic acetylcholine receptor and transient receptor potential channels. The fundamental involvement of these channels on the generation of obesity leads to the progress in the knowledge about the mechanisms responsible for the obesity pathophysiology, consequently emerging as new targets for pharmacological modulation.


INTRODUCTION
Obesity is an important disease associated to the excessive accumulation of body fat, leading to weight gain and the development of chronic disorders, including arterial hypertension, type 2 diabetes mellitus, dyslipidemias, sleep apnea, and cancer (Jeffreys et al., 2003;Van Gaal et al., 2006;Pischon et al., 2008;Whitlock et al., 2009;Vucenik and Stains, 2012;Singh et al., 2013). Currently, it affects 35% of adults aged 20+ with a rising prevalence (World Health Organization [WHO], 2014).
Multiple factors are involved in the origin of this disease, involving genetic determinants, as well as environmental, psychosocial and psychobiological factors (Comuzzie and Allison, 1998;Bocchieri et al., 2002;Noll et al., 2007). Meanwhile, regardless of its cause, the body weight results from a complex interaction between physio-and psychological components that control food intake and energy expenditure (Martinez, 2000).
Food intake is regulated centrally and it is influenced by the phenomena of hunger, satiety and appetite. Neurons located in several regions of the hypothalamus (ventromedial, lateral hypothalamic, arcuate and paraventricular nuclei) and in extra hypothalamic regions, including hindbrain (nucleus tractus solitarii), midbrain (ventral tegmental area) and forebrain (nucleus accumbens), are associated with the energetic homeostasis in human (Thaler et al., 2013).
Specifically, the arcuate nucleus (ARC) in the mediobasal hypothalamus contains different types of neurons correlated to the expression of orexigenic peptides, such as neuropeptide Y (NPY) and agouti-related peptide (AgRP), which stimulate food intake and reduce the body energy expenditure. In contrast, neurons expressing anorexigenic peptides derived from proopiomelanocortin (POMC), cocaine and amphetamine regulated transcript (CART) trigger opposite effects, such as decreased food intake and increased body energy expenditure (Bing et al., 1996;Schwartz et al., 2000;Broberger, 2005;Huda et al., 2006). In addition, the nucleus tractus solitarii (nTS) receives gustatory and visceroceptive signals, especially from vagal afferents synapse, and sends projections to different brain regions in order to regulate the food intake (Harding and Leek, 1973;Appleyard et al., 2005). Recently, several studies have suggest a role of ion channels in the regulation of synaptic function in the brain associated to the increase/decrease of expression of orexigen/anorexigen peptides and the energetic homeostasis (Olds and Xu, 2014).
Leptin is a key adipokine in the regulation of energy intake/expenditure that shows concentrations proportional to body fat mass (Sinha and Caro, 1998). Leptin acts on the ARC stimulating the anorexigenic neurons and inhibiting the orexigenic ones (Druce and Bloom, 2006). Another important event promoted by this adipokine is the induction of posttranslational processing of the precursor molecule of POMC, leading to the production of melanocortin peptides that present anorexigenic property (Broberger, 2005). Mutations affecting the leptin-melanocortin pathway involving genes encoding leptin and/or its receptor and the prohormone convertase enzyme that processes the POMC constitute the more present genetic alterations in obese (Farooqi and O'Rahilly, 2008). Ghrelin is produced in the stomach (Tschop et al., 2000;Wiedmer et al., 2007) and is the only peripheral hormone that stimulates the expression of orexigenic neuropeptides. Thus, it is known to induce increase in hunger and food intake (Huda et al., 2006). Before meals, the levels of ghrelin are raised, and after the ingestion of nutrients, its levels are suppressed (Tschop et al., 2001). Additionally, adiponectin is a hormone which is synthesized, exclusively, by adipocytes and acts inhibiting the hepatic glucose production, while increasing glucose uptake in muscle, as well as the oxidation of fatty acids in the liver and muscles, causing energy expenditure (Scherer et al., 1995;Kadowaki and Yamauchi, 2005). Therefore, obese individuals present low levels of adiponectin (Lindsay et al., 2002;Hajer et al., 2007).
The adipose tissue has the ability to store excess calories resulting in the process of hypertrophy and/or hyperplasia (Ferranti and Mozaffarian, 2008). The deposition and release of fatty acids from adipose tissue (as triglyceride) alters the food intake and energy expenditure. Moreover, different studies showed that adipose tissue is generally composed by 50% of adipocytes and the remaining 50% are cells such as preadipocytes, vascular cells, neural, leukocytes and cells of the immune system (Gustafson, 2010;Maenhaut and Van de Voorde, 2011). Based on functional and morphological differences, this tissue was divided into two main types, white adipose tissue (WAT) and brown adipose tissue (BAT). The WAT acts as an energy storage and is an active endocrine organ, releasing the free fatty acids and adipokines. Conversely, the BAT presents cells which are characterized by multilocular lipid droplets and an increased number of mitochondria, which express uncoupling protein 1 (UCP1), which uncouples the rates of substrate oxidation and ATP production by favoring a loss of protons and thus energy release. Thus, BAT is associated with thermogenesis (Tam et al., 2012).
Regarding the diagnosis of obesity, anthropometric criteria are the most often used, such as body mass index (BMI), measurement of waist circumference, waist-hip ratio, and skinfold test with the use of tweezers to evaluate the percentage of fat (Racette et al., 2003;McArdle et al., 2008). Additionally, there are other techniques for the assessment of body weight, including electrical bioimpedance (BIA), hydrostatic weighing and dual-energy X-ray absorptiometry (DEXA): BIA evaluates the resistance to a low frequency electrical current, considering that the current travels through the aqueous compartments and not through the greasy ones, where the flow impediment occurs (Kushner and Schoeller, 1986). The hydrostatic weighing determines the total volume of the body by analyzing the difference between the weight of an object in water and air, determining the density of the whole body (Schoeller et al., 1980). In DEXA analysis, the composition of soft body tissues is measured, by using the ratio of absorption of X-rays of different energies attached to adipose tissue mass (Kohrt, 1998).
Obesity treatment is based on multiple approaches that may encourage behavior change in long term, through energyreduced diets, physical activity and/or exercise, pharmacotherapy and surgery (National Institutes of Health [NIH], 1991[NIH], , 2000Rosenbaum et al., 1997;Klein, 1999;Bray and Tartaglia, 2000). There is a consensus among the National Institutes of Health, the National Heart, Lung and Blood Institute and the North American Association for the Study of Obesity that the choice of treatment depends on the degree of obesity and the presence of comorbidities. Accordingly, for BMI ≥ 25 kg/m 2 , changes in lifestyle are recommended (physical activity, diet, and behavior), for BMI into 27.7-29.9 kg/m 2 , in the presence of comorbidities or for BMI ≥ 30 kg/m 2 , in the absence of comorbidities, the use of pharmacotherapy is recommended. The surgery for weight reduction is recommended for patients with severe obesity (BMI > 35 kg/m 2 ) in the presence of comorbidities, or individuals with a BMI > 40 kg/m 2 in the absence of comorbidities (National Institutes of Health [NIH], 2000).
Nowadays, the treatment of obesity remains a challenge. Most anti-obesity drugs currently prescribed target appetite regulation or decreasing in the absorption of nutrients (Bays, 2004). Most of them have significant side effects, and do not act specifically on regulatory functions of appetite in the hypothalamus, but exhibit a broad spectrum of actions. In many cases, beneficial effects remain limited (Heymsfield et al., 1999).
The most prescribed drugs for the treatment of obesity are sibutramine (serotonin and norepinephrine reuptake inhibitor) and orlistat (gastrointestinal lipase inhibitor), both prescribed for long-term use (Cannon and Kumar, 2009). The use of sibutramine for weight reduction has favorable effects on risk factors for cardiovascular disease (increasing high-density lipoprotein and decreasing levels of cholesterol and triglycerides in the blood; Dujovne et al., 2001). However, the use of this drug is contraindicated in patients with uncontrolled hypertension, coronary artery disease, cardiac arrhythmias, congestive heart failure or stroke, because it can increase blood pressure and heart rate (Kim et al., 2003). It is recommended the use of orlistat associated with multivitamin supplements, considering that orlistat affects the absorption of fat-soluble vitamins (A, D, E, and K) that are essential for the body (Cannon and Kumar, 2009). In obese patients, the benefits of this drug use is associated with lower incidence of type 2 diabetes, reduction of total cholesterol, LDL cholesterol and triglyceride levels as well as the levels of glycated hemoglobin (HbAlc) in diabetic (Aronne et al., 2009). Amfepramone (diethylcathinone) and fenproporex are also inhibitors of appetite, which act on the hypothalamic neurons, increasing the release of norepinephrine and stimulating the noradrenergic receptors, then, inhibiting hunger (Zaragoza et al., 2005;Cohen, 2009). Other medications have been evaluated in obese individuals, however, without formal indication for obesity treatment.
Because of ethical limitations on studying the susceptibility of human obesity, animal models of obesity has been proposed to investigate the pathophysiological changes caused by this disease (Pereira et al., 2003). Obesity has been induced in animals by nerve injury, endocrine disorders, genetic, and/or dietary modifications (Sclafani and Springer, 1976), which facilitates the study of the pathophysiology of obesity and allows advances in the development of new therapies.
Therefore, in view of the influence of obesity in the development of chronic diseases affecting the life expectation and the limitations of the current available treatment, this revision focused on the involvement of ion channels in the pathophysiology of obesity, as well as the role of these targets as new approaches toward the development of new anti-obesity drugs.

ION CHANNELS IN THE DEVELOPMENT OF OBESITY
Ion channels play fundamental roles in diverse key functions, such as maintenance of physiological homeostasis, cell proliferation and signal transduction in a variety of cell types and different cell stages (Nilius and Droogmans, 2001;Chen et al., 2007). Cell biology and pharmacological experimental evidences demonstrate that proliferating cells exhibit ion channel expression, conductance, and electrical properties which are very different from the resting cells (Conti, 2007). In this context, the investigation of ion channels has been emerging as a new approach in the study of the pathogenesis of obesity.

Ion Channels on Adipose Cell Proliferation
Obesity development results from the expansion of WAT and this process depends of stem cell proliferation (Gray and Vidal-Puig, 2007). This process is dependent of ion channels functionality (Chen et al., 2007). It is well-known that K + channels are involved in cell proliferation, as the EGF-mediated mitogenic signal transduction process, required for voltagegated K + channels participating in G 1 /S-phase transition of the cell cycle (MacFarlane and Sontheimer, 2000). Previous studies reported the presence of voltage-gated K + currents (K V ) in BAT isolated from the interscapular fat pads of neonatal rats (Lucero and Pappone, 1989;Russ et al., 1993), WAT differentiated from rats (Ramirez-Ponce et al., 1996) and/or human preadipocytes (Ramirez-Ponce et al., 2003). Additionally, Bai et al. (2007) identified three types of K + currents on human adipose tissue-derived stem cells (hASCs): a delayed rectifier-like K + current (I KDR ), a Ca 2+ -activated K + current (I KCa ) and a transient outward K + current (I to ). In addition, the mRNA of K V 1.1, K V 1.5, K V 2.1, K V 7.1, K V 10.1, and K V 11.1 has been shown to correspond to I KDR ; mRNA of MaxiK (BK Ca ), KCNN3 (SK Ca 3) and KCNN4 (SK Ca 4), corresponding to I KCa , and high mRNA levels of K V 1.4, K V 4.2, and K V 4.3, contributing to I to . However, despite the expression of these different K + channels in hASCs, only the I KDR current has been associated to its proliferation ( Table 1). Hu et al. (2009) showed the crucial participation of K + channels in obesity. The knockdown or blockade with 4-aminopyridine and paxilline of K Ca 1.1 and K V 4.2 channels, respectively, related to I to , increased the cell number of human preadipocytes on G 0 /G 1 phase, preventing cell cycle progression. Thus, these data indicate the importance of K + channels activity to preadipocytes proliferation and differentiation, resulting in obesity installing. Additionally, in brown adipocytes culture obtained from rat, it has been demonstrated that the blockade of K V channels with tetraethylammonium inhibits cell proliferation (Pappone and Ortiz-Miranda, 1993). The brown adipocytes are connected to metabolic energy expenditure and, therefore, are important to prevent fat accumulation. Thus, a balance in the activity of white and brown fat cells can be seen, regulated by K + channels, that prevent fat deposition. It Regulates response of taste cells to fat ingestion and the leaning to high fat intake Gilbertson et al., 2005 is still not clear the role of K + channels in the genesis of obesity. The data presented here ponder a similar role of these channels on the proliferation of both white and brown preadipocytes. It becomes important, therefore, to determine which of these subtypes are predominant for the evolution of the cell cycle in those two cell types and how its dysfunction would lead to the development of obesity ( Table 1).
Other ion channels have been associated with proliferation of preadipocytes. On 3T3-L1-preadipocytes, an establish a model for adipocyte differentiation, and visceral adipose tissue from both mice and human, Zhang et al. (2007) have detected the expression of TRPV1 and have shown that TRPV1 are important in preventing the proliferation and differentiation of preadipocytes. Accordingly, the treatment with capsaicin, a TRPV1 agonist, prevented the differentiation of these cells. Likewise, it has been evidenced the TRPV1 downregulation during adipogenesis of preadipocytes as well as on visceral adipose tissue from db/db and ob/ob mice, suggesting that TRPV1 function prevents adipogenesis and consequently obesity (Miller et al., 1996;Neal and Clipstone, 2002) (Table 2). Additionally, Hu et al. (2010) have detected Cl − currents on human abdominal subcutaneous adipose tissue. Furthermore, it was verified the expression of chloride channel-3 (CIC-3) on this tissue and its blockade with tamoxifen reduced cell proliferation, suggesting the role of Cl − channel in regulation of human preadipocyte proliferation.
Therefore, all these data directs to a probable requirement for hyperpolarized state of preadipocytes, which can be achieved by the efflux of K + , inhibition of cation influx or even by Cl − influx, to the progress of these cells in the cell cycle until reaching the needed maturity state for their proliferation and differentiation. Accordingly, drugs that prevent this progression by acting on ion channels involved could provide new tools for obesity therapy.

Ion Channels on Central Food Intake Control
Some K + channels have been shown to have an important role in the central regulation of food intake, energy expenditure, and glucose metabolism. The K ir 6.2, suggested being the K ATP pore-forming subunit (Karschin et al., 1998;Zawar et al., 1999;Miki et al., 2001), is expressed in neurons of ventromedial hypothalamic nucleus (VMH), POMC and melanin-concentrating hormone (MCH) neurons of lateral hypothalamic area (LHA) of mice and mediates neuronal glucose-excitation, acting as a glucose sensor during feeding. The defective K ir 6.2 prevents ATP-mediated K ATP blocking, leading to impaired glucose responsiveness by POMC neurons, resulting in glucose intolerance (Sohn, 2013). Additionally, this event has been observed in obese mice on a highfat diet, resulting in loss of glucose sensitivity by these neurons, contributing to food intake overstimulation and obesity progress (Miki et al., 2001). These data corroborate Rowe et al. (1996), who showed that obese rats exhibit abnormal electrophysiological responses in hypothalamic glucose-sensors neurons to changes on extracellular glucose concentration, whereas lean rats respond normally. The role of K ATP on regulating food intake behavior has been also shown to be important in preventing age-dependent obesity. It was shown that rapamycin-sensitive (mTOR) signaling is elevated in POMC neurons of old mice, causing silencing of these neurons and inhibition of leptin-induced release of the anorexigenic α-MSH, associated with upregulation of K ATP channel activity and an aging-dependent high expression of K ir 6.2 (Yang et al., 2012) ( Table 1). Leptin and insulin are well-recognized as anorexigenic hormones released from adipocytes and pancreatic beta cells, respectively (Williams et al., 2011b). Typically, leptin activates POMC neurons and leptin receptor (LepR)-expressing neurons of ventral premammilary nucleus (PMV), known to release of anorexigenic hormones (Cowley et al., 2001;Al-Qassab et al., 2009;Williams et al., 2010Williams et al., , 2011a. However, their effects on ion channels in the CNS are yet controversial. In the VMH, insulin receptors expressed by the SF-1 neurons activate K ATP channels and suppress steroidogenic factor 1 (SF1) neuron activity, which resulted in diet-induced obesity (Klockener et al., 2011). In addition, insulin activates some arcuate NPY/AgRP neurons, which release orexigenic hormones, leading to hyperphagia state (Al-Qassab et al., 2009). Melanocortin is another hormone important in regulating body fat accumulation. Mutations on MC4R has been shown to induce obesity in rodents and humans (Huszar et al., 1997;Vaisse et al., 1998;Tallam et al., 2005), and its activation hyperpolarizes parasympathetic preganglionic neurons in brainstem via PKAdependent activation of tolbutamide-sensitive K ATP channels and depolarize the sympathetic ones, which could be one of the factors responsible to inducing obesity-associated hypertension.
However, this data is more associated with an obesity consequence than a cause. The ion channels involved in melanocortin pathways to regulate food intake and obesity development have not yet been determined. Therefore, the effects of these hormones on ion channels activity in neurons that regulate food intake deserve an interesting focus for future insights attempting to better understand their relationship with obesity. Meanwhile, K ATP channels appear to be promising targets to treatment of obesity, focusing in drugs acting in CNS neurons regulating food intake ( Table 1). K V 1.3 has been implicated in the regulation of energy homeostasis, body weight and insulin resistance (Xu et al., 2003(Xu et al., , 2004. This channel was shown to act as a metabolic sensor responding to insulin levels in mitral cells of olfactory bulb, and its removal increases the weight gain (Marks and Fadool, 2007;Biju et al., 2008). Mitral cells of the olfactory bulb function as internal chemical sensors of metabolic state by modulating K V channels predominantly expressed in these neurons (Fadool et al., 2000). K V 1.3 knockout mice fed a high-fat diet exhibit increased light-phase metabolism in addition to reduced weight gain, reduced levels of sugar, leptin and insulin in the blood, and increased energy expenditure (Xu et al., 2003(Xu et al., , 2004Li et al., 2006;Tucker et al., 2008;Fadool et al., 2011;Tucker K.R. et al., 2012). Additionally, these knockout exhibited metabolic alterations including increase in energy expenditure and locomotor activity, resistance to diet-and genetic-induced obesity and increased insulin sensitivity (Fadool et al., 2004;Xu et al., 2004;Tucker et al., 2008). Therefore, linking to the role of K ATP channels in hypothalamic areas to regulate food instinct, the K V 1.3 channels seem to have an important role at the CNS too. These channels appear to be important in regulating food drive through the olfactory effects which are responsible for stimulating food intake ( Table 1).
Nicotinic receptors have been shown to have an important role to prevent fat accumulation and to induce fat degradation. In a model of type 2 diabetes, the homozygous leptin-resistant db/db obese mouse, Marrero et al. (2010) measured the effects of a novel α7 nAChR-selective agonist, TC-7020, and showed reduction of food intake and weight gain. These parameters were reversed by using a janus kinase 2 (JAK2) specific inhibitor (AG-290), demonstrating that the α7 nAChRs plays an important role in the body weight control and it involves JAK2 signal transducer and activator of transcription 3 (STAT3) signaling pathways. Similarly, C57BL/6J mice with a high-fat diet treated with galantamine, an acetylcholinesterase (AChE) inhibitor that enhances cholinergic signaling and also acts as a positive allosteric modulator of a7 nAChR, presented reduction on food intake, body weight and abdominal adiposity as well as an improvement on blood glucose, insulin resistance, and hepatic steatosis (Satapathy et al., 2011) (Table 3).
Cigarette smoking habits have been associated with less body fat distribution, body weight, insulin resistance and obesity (Chiolero et al., 2008;Clair et al., 2011). Indeed, the smoking cessation increases body weight (Perkins, 1993). Experimentally, nicotine administration to rodents and humans suppresses appetite, increases energy expenditure and alters feeding behavior Fornari et al., 2007;Zoli and Picciotto, 2012). The mechanisms involved in nicotineinduced decrease in body weight have been correlated to both central and peripheral nicotinic cholinergic signaling. In CNS, nAChRs composed by both α7 and α/β subunits were detected throughout areas of the hypothalamus that regulate appetite, food consumption and body mass. In previous studies, it was revealed from moderate to high levels of α4, α7, and β2-mRNAs in hypothalamus, with particularly prominent expression in the supraoptic or suprachiasmatic nuclei and lateral hypothalamus . Moreover, although the α3β4 nAChR is the predominant nicotinic receptor in the peripheral nervous system, it is less widely distributed in the rat brain (Dwyer et al., 2008) and has been related to nicotine effects on mice hypothalamus to decrease food intake. In particular, the activation of α3β4 nAChRs in POMC cells in the ARC decreases food intake and increases energy expenditure (Williams and Schwartz, 2005;Mineur et al., 2011) ( Table 3).
The gamma-aminobutyric acid type A receptor (GABA A ) has been associated to the body weight regulation. Tong et al. (2008) showed that inhibition of GABA release from AgRP neurons of mice prevents obesity. Additionally, Vong et al. (2011) showed a lean phenotype in the presence of defective synaptic release of GABA from NPY/AgRP neurons. This effect was associated to a decrease on inhibitory post-synaptic currents (IPSCs) in anorexigenic POMC neurons. Beyond this, Wu et al. (2009) have showed that inhibition of GABA A receptor with the antagonist

Localization
Species nAChRs (gene) Comments Reference

Ion Channels on Sleep-Vigilant Cycle Dysfunction-induced Obesity
There are few studies linking sleep and metabolism, being this interplay only currently receiving attention. In a study made by Uebele et al. (2009) using mice with deletion of Ca V 3.1 channels (Cacna1g KO mice), a model that promotes fragmented sleep, the potential link between sleep and obesity was investigated. The authors found that KO mice presented less weight gain and amount of fat after inducing them to a high-fat diet (HFD), compared to the wild type mice (WT). Moreover, it was showed that the resistance on weight gain observed to KO mice was due to metabolic rate changes in these animals, in a manner that did not affect core body temperature. Furthermore, the authors assessed a possible similar effect using a T-type Ca 2+ channel antagonist (TTA-A 2 ) and, initially, they observed that the inhibition of T-type Ca 2+ of WT mice caused sedation and decreased on active wake, consistent with altering thalamocortical neuronal activity. Additionally, these WT mice presented less weight gain after inhibition of T-type Ca 2+ and, interestingly, this difference was observed only when the mice received HFD. All the results were similar to that observed on Cacna1g KO mice. Additionally, animals fed with HFD presented reduced food intake, not on the active phase, but in the inactive phase. These effects probably result from a better alignment of diurnal feeding patterns with daily changes in circadian physiology and, potentially, an increased metabolic rate during the active phase. Thus, these data suggest a role for Ca V 3.1 in co-regulating sleep and weight maintenance and data from pharmacological studies demonstrate that potent and selective T-type calcium channel antagonists reduce-wakefulness, diet-induced weight gain, and improve-body composition, suggesting this ion channel class may provide a novel therapeutic target for the treatment of obesity.
Additionally, it has been shown that elevated glucose levels, reached, e.g., after meal, activate a two-pore domain K + channel (K2P) and inhibits orexin/hypocretin release by LHA neurons, regulating the sleep-vigilant cycle (Burdakov et al., 2006). Thus, impaired K2P function can emerge as a reason of hyperphagia promoted by release of high amounts of this hormone associated with sleep-vigilant cycle dysfunction (Table 1).
Therefore, there must be an interplay between Ca 2+ and K + channels that regulates sleep-vigilant cycle and food intake as well, emerging as interesting targets in the CNS to treat obesity, especially the sleeplessness.

Ion Channels on Peripheral Food Intake Control
Free fatty acids are able to activate peripherally taste receptor cells (TRCs; Fukuwatari et al., 2003), the stimulus involves an interaction between the tastant and ion channels or receptors localized in most cases on the apical membranes of the TRCs. The initial events in the taste transduction of free fatty acids include an inhibition of delayed rectifying potassium channels (DRK) associated with K V 3.1 channels (Gilbertson et al., 1997(Gilbertson et al., , 1998(Gilbertson et al., , 2005. Gilbertson et al. (2005) have demonstrated that DRK currents are inhibited in obese-resistant rats (O-R), but not in obesity-prone (O-B) test cells, in response to polyunsaturated fat acids (PUFAs), and this effect was associated with the greater activation of taste cells in O-R than that in O-B. Additionally, TRCs in O-R would be activated to a greater degree by PUFAs than those from O-B. Likewise, the DRK channels were not found in O-R, indicating that PUFAs do not inhibit K + currents by these channels in O-B, and they are the most responsible for the taste cells inhibition in obese rats, which might explain the preference of these obese rats to a high fat dietary, while the resistant-rats tend to eat much less ( Table 1).
TRPM5 is another ion channel abundantly identified in TRCs which participates in sweet, umami, and bitter perception. The disruption of this channel gene in mice abolishes the transduction of these tastes (Pérez et al., 2002;Zhang et al., 2003). TRPM5 (−/−) mice were shown to present the same pattern of food intake and gained less weight than wide-type mice when fed a high-sugar diet, being this effect probably due to the loss of sucrose preference (Glendinning et al., 2012). Interestingly, these data corroborate Gilbertson et al. (2005), as showed previously, that demonstrated that inhibited state of taste cells leads to obesity sensitivity. Additionally, TRPM5 (−/−) mice also abolished the preference for fat and reduced the fatty acid-induced [Ca 2+ ] c increase, suggesting that TRPM5 also plays a key role in lipid taste perception (Oike et al., 2006;Sclafani et al., 2007;Liu et al., 2011) ( Table 2).

Ion Channels on Body Energetic Expenditure
Some ion channels have been associated with the regulation of energetic expenditure. The nicotinic cholinergic signaling, in special, in metabolic tissue, is one of them. Liu et al. (2004) have shown that α1-7, 9-10, β1-4, δ and ε subunit mRNAs are expressed in rat adipocytes, and the release of FFA by this cell has been known to increase nicotine concentration. Recent studies of Somm et al. (2014) have shown that in mouse WAT, α2 and β1 were the most expressed nAChR subunits. Meantime, mouse BAT expresses α2 and comparable amounts of β1 and β2 nAChR subunits. Then, although in a α7β2 nAChR (−/−) mice model it was verified the unaltered body weight, a decreased fat storage was observed. Additionally, Cancello et al. (2012) have demonstrated the expression of α7 nAChR in isolated mature adipocytes and human subcutaneous adipose tissue (SAT). Meanwhile, the expression of this receptor was associated with decreased SAT obtained from obese human compared to normal-weight human. In addition, α7 nAChR had 75% lower expression in mature adipocytes isolated from morbidly obese human compared to adipocytes from normal-weight human. These receptors modulates inflammatory gene expression in human adipocytes and are linked to adipocyte lipid catabolism ( Table 3).
In human studies, polymorphisms in genes encoding nAChRs have been focused to examine the potential involvement of nicotinic receptors variants on the distribution of body fat. The SNP in the nAChR α2 subunit gene (CHRNA2) was associated with overweight/obesity (Kim, 2008). There was no association between the BMI and the genotype of 15q25 SNP (CHRNA5-CHRNA3-CHRNB4 gene region) in the never smokers, however, in smoking individuals it was observed an association between the 15q25 variant and BMI, highlighting the smoking habit as a probable cause of reduced BMI and obesity resistance (Freathy et al., 2011;Zhu et al., 2013) (Table 3).
Regarding TRP channels, genetic approaches of human TRPV1 showed a SNP, Val585Ile, associated with weight loss. It was showed that the presence of Val/Val and Val/IIe variants leads to the loss of twice as much abdominal fat in response to vanilloids, as those with IIe/IIe (Hayes et al., 2000;Snitker et al., 2009). Indeed, the energy intake is decreased in humans that consume red chili pepper (Yoshioka et al., 1999) and the repeated intake of CH-19 Sweet, a non-pungent red pepper, reduces the body weight and suppresses body fat accumulation due to the sympathetic nervous activation, which leads to fat acids mobilization (Kawabata et al., 2006). The balance of the literature suggests that both capsaicin and capsiate augment energy expenditure and enhance fat oxidation, especially at high doses (Ludy et al., 2011). In addition, Ma et al. (2012) showed a functional TRPM8 expression on BAT, associated with metabolic energy expenditure and the use of menthol, a TRPM8 activator, increases the expression of UCP1, located in the inner mitochondrial membrane, responsible for thermogenesis in BAT (Vogler et al., 2008). TRPM2 has also been shown to be involved in the energy expenditure as evidenced for Zhang et al. (2012), but conversely, TRPM2 (−/−) mice were resistant to obesity after 4-10 months of high-fat feeding. These animals presented greater energy expenditure and elevated expression of lipid metabolic genes in WAT. Additionally, white adipocytes from TRPV4 (−/−) mice fed a high-fat diet were smaller and exhibited enhancement in the energy expenditure, due to the elevated levels of UCP1 expression. Thus, Ye et al. (2012) suggest that TRPV4 negatively regulates oxidative metabolism. In addition, Kusudo et al. (2012) have shown that inactivation of the TRPV4 gene induces an increase in TRPC3 and TRPC6 expression and calcineurin activity in mice. The energy metabolism was altered due to the expression of genes involved in fuel oxidative in skeletal muscle, contributing to the resistance to obesity. Likewise, Tamura et al. (2012) have reported that the supplementation with cinnamaldehyde, a TRPA1 agonist, increased the UCP1 protein levels in the interscapular BAT and reduced the visceral fat in mice fed with diet rich in fat or sucrose. Therefore, these data support the key role of TRPs on regulating energy expenditure involving specially the activity of BAT ( Table 2).
It has also been extensively reported that intracellular Ca 2+ ([Ca 2+ ] i ) appears to be involved in metabolic derangements, including obesity and insulin resistance. It was previously reported that agouti-induced obese mice present high basal [Ca 2+ ] i as well as increased Ca 2+ influx rate, also associated with high expression and activity of fatty acid synthase (FAS) and increased triglycerides accumulation in 3T3-L1 adipocytes (Zemel et al., 1995). Interestingly, FAS activity has been prevented by Ca 2+ channel blockade with nitrendipine (Jones et al., 1996). Moreover, it was reported that chronic hyperinsulinemia induces an increase in FAS mRNA level and activity in both rat liver and WAT (Chakrabarty and Leveille, 1969). In this context, Kim et al. (1996) reported that treatment of obese mice with nifedipine normalized both FAS mRNA and plasma insulin levels, associated with a reduction of fat pad mass in agouti-induced obese mice, together with an increase in the skeletal muscle weigh increase, which can be associated with the use of energy from adipose store by muscle cells. Indeed, it was also observed that the treatment corrected the reduction in core temperature of obese mice, which can be explained by the increase in energy expenditure by skeletal muscle. This effect was associated with a possible reduction on Ca 2+ levels on pancreatic β-cells, reducing the insulin secretion, or due to an improvement in insulin sensitivity on these mice. Alternatively, it is not discarded that Ca 2+ can acts in transcriptional level to increase the FAS expression. Therefore, nifedipine was able to reduce lipogenesis and insulin resistance in obese mice. Early evidence has already been reported, according to which the anti-obese effect of a Ca 2+ channel blocker, benidipine, which reduced mice body weight associated with the increase in blood flow to BAT, functions as the main thermogenic organ (Yoshida et al., 1994).
Together, these data bring interesting information regarding nicotinic, TRPs and calcium channels as key ion channels responsible for regulating energy expenditure. Therefore, new drugs targeting these channels could emerge as new approaches in the field of obesity treatment.

Ion Channels on Accelerated Gastric Emptying Leading to Overeating
The accelerated gastric emptying and thus reduced nutrient absorption has been linked with food overconsumption and obesity development. Nozawa et al. (2009) have showed the expression of TRPA1 in the gastrointestinal tissues and enterochromaffin cells (ECs) in humans, mice and rats. The stimulation of TRPA1 by allyl isothiocyanate (AITC) and cinnamaldehyde increased 5-hydroxytryptamine (5-HT) release from EC cells, which is known to accelerate the gastric emptying in human and it is known to the overconsumption on a HFD and subsequent obesity (Wright et al., 1983;Zahorska-Markiewicz et al., 1986;Tosetti et al., 1996) (Table 2). Furthermore, there are reports suggesting that gastric emptying of solid food is accelerated in obese subjects, which precipitates hunger and frequent eating (Wright et al., 1983;Tosetti et al., 1996). In obese humans, accelerated gastric emptying may be one of the contributing factors to overconsumption on a HFD and subsequent obesity (Wright et al., 1983;Zahorska-Markiewicz et al., 1986;Tosetti et al., 1996). In this context, Li et al. (2013) reported Ca 2+ and K + currents in antral circular smooth muscle cell. The diet-induced obese-prone rats exhibit accelerated gastric emptying attributed to a higher density of L-type Ca 2+ currents (I Ba,L ) and inactivation or low density of K + currents, being them the responsible for the high Ca 2+ currents amplitude in these cells and thus increasing gastric emptying, leading to overconsumption and obesity.

PERSPECTIVES
In view of the large amount of information regarding the involvement of ion channels in the obesity development, as well as the development of suitable animal models, a new unexplored aspect arises to establish novel alternatives for obesity treatment having as targets deregulated molecular aspects in the genesis of this disease. Various ion channels have been identified in several cell types as important regulators of some functions related to the development of obesity. The K + channels were the most well-studied and have been associated with the proliferation of adipose cells, central and peripheral regulation of food intake and gastric emptying time, all preponderant for the development of obesity. Other channels, such as TRPs and nicotinic receptors, are involved in many of these functions. The Ca 2+ channels, although still not better explored, has been associated particularly in unusual obesity factors, such as disruption of the sleep-wake cycle, as well as chloride channels not yet explored, but identified in some cell types involved in obesity. In this sense, further research targeting important ion channels in the pathogenesis of obesity are promising for the development of more effective drugs to treat the disease. Accordingly, drugs that act on the ion channels described, specially K + channels, may be promising to the further treatment of obesity.

AUTHOR CONTRIBUTIONS
LV, IS, and LP made the major part of research, designed and write the manuscript; BdS guided the preparation of the work.