Profile of Antiemetic Activity of Netupitant Alone or in Combination with Palonosetron and Dexamethasone in Ferrets and Suncus murinus (House Musk Shrew)

Background and Aims: Chemotherapy-induced acute and delayed emesis involves the activation of multiple pathways, with 5-hydroxytryptamine (5-HT; serotonin) playing a major role in the initial response. Substance P tachykinin NK1 receptor antagonists can reduce emesis induced by disparate emetic challenges and therefore have a clinical utility as broad inhibitory anti-emetic drugs. In the present studies, we investigate the broad inhibitory anti-emetic profile of a relatively new NK1 receptor antagonist, netupitant, alone or in combination with the long acting 5-HT3 receptor antagonist, palonosetron, for a potential to reduce emesis in ferrets and shrews. Materials and Methods: Ferrets were pretreated with netupitant and/or palonosetron, and then administered apomorphine (0.125 mg/kg, s.c.), morphine (0.5 mg/kg, s.c.), ipecacuanha (1.2 mg/kg, p.o.), copper sulfate (100 mg/kg, intragastric), or cisplatin (5–10 mg/kg, i.p.); in other studies netupitant was administered to Suncus murinus before motion (4 cm horizontal displacement, 2 Hz for 10 min). Results: Netupitant (3 mg/kg, p.o.) abolished apomorphine-, morphine-, ipecacuanha- and copper sulfate-induced emesis. Lower doses of netupitant (0.03–0.3 mg/kg, p.o.) dose-dependently reduced cisplatin (10 mg/kg, i.p.)-induced emesis in an acute (8 h) model, and motion-induced emesis in S. murinus. In a ferret cisplatin (5 mg/kg, i.p.)-induced acute and delayed emesis model, netupitant administered once at 3 mg/kg, p.o., abolished the first 24 h response and reduced the 24–72 h response by 94.6%; the reduction was markedly superior to the effect of a three times per day administration of ondansetron (1 mg/kg, i.p.). A single administration of netupitant (1 mg/kg, p.o.) plus palonosetron (0.1 mg/kg, p.o.) combined with dexamethasone (1 mg/kg, i.p., once per day), also significantly antagonized cisplatin-induced acute and delayed emesis and was comparable with a once-daily regimen of ondansetron (1 mg/kg, p.o.) plus aprepitant (1 mg/kg, p.o.) in combination with dexamethasone (1 mg/kg, i.p.). Conclusion: In conclusion, netupitant has potent and long lasting anti-emetic activity against a number of emetic challenges indicating broad inhibitory properties. The convenience of protection afforded by the single dosing of netupitant together with palonosetron was demonstrated and also is known to provide an advantage over other therapeutic strategies to control emesis in man.


INTRODUCTION
The treatment of cancer with chemotherapeutic agents such as cisplatin is documented to be associated with a number of side effects including nausea and emesis, which can be reduced by agents blocking 5-HT 3 and substance P NK 1 receptors Hesketh, 2008). It has been hypothesized that there is an initial release of 5-HT (serotonin) from enterochromaffin cells in the gastrointestinal tract to activate 5-HT 3 receptors located on vagal afferents Minami et al., 2003). The mechanism of release is not entirely known but may involve free radical generation and/or cellular damage, which subsequently leads to the involvement of other neurotransmitter systems and/or mediators (Andrews and Rudd, 2015). The response occurring over the first 0-24 h has become known as the acute response, and 'first generation' 5-HT 3 receptor antagonists, such as ondansetron and granisetron, have been used widely to reduce nausea and emesis during this phase (Hesketh, 2008). However, 5-HT 3 receptor antagonists are less effective to control nausea and emesis occurring during the post 24 h period, which became known as the delayed phase of emesis .
To improve the overall control of acute and delayed emesis, 5-HT 3 receptor antagonists were initially combined with glucocorticoids such as dexamethasone (Hesketh et al., 1994;Ioannidis et al., 2000). Subsequently, preclinical studies identified that brain penetrating tachykinin NK 1 receptor antagonists had a broad inhibitory profile to inhibit emesis induced by disparate challenges (Bountra et al., 1993;Tattersall et al., 1993Tattersall et al., , 1994Andrews and Rudd, 2004). It was also shown that NK 1 receptor antagonists had anti-emetic activity in a ferret model of acute and delayed emesis prompting clinical testing Singh et al., 1997;Tattersall et al., 2000;Tsuchiya et al., 2002). The standard regimen to control both phases of emesis in man quickly changed to a triple regimen of a 5-HT 3 receptor antagonist, in combination with the first licensed NK 1 receptor antagonist for chemotherapy-induced emesis, aprepitant, plus a glucocorticoid (Jordan et al., 2005;Roila et al., 2010;Bayo et al., 2012). Unfortunately, however, even with these advances, there still remains a proportion of patients not adequately protected from chemotherapy-induced nausea and emesis (Navari, 2004;Aranda Aguilar et al., 2005).
Palonosetron is a 'second generation' 5-HT 3 receptor antagonist that is an order of magnitude more potent than older compounds at blocking 5-HT 3 receptors and has excellent bioavailability following oral administration (F 99%); it also has a plasma half-life that is approximately three times longer, enabling a convenient once per day administration (Wong et al., 1995;Grunberg and Koeller, 2003;De Leon, 2006). In the clinical setting, palonosetron was shown to be superior to the first generation 5-HT 3 receptor antagonists, particularly in its ability to reduce delayed nausea and emesis (Rubenstein, 2004;Geling and Eichler, 2005;Tonini et al., 2005). It was speculated that the unique profile of palonosetron may relate to its additional ability to prevent 5-HT 3 receptor re-cycling and/or a potential to reduce substance P responses mediated via NK 1 receptors by preventing 5-HT 3 and NK 1 receptor cross-talk (Rojas et al., 2008(Rojas et al., , 2010b(Rojas et al., , 2014Stathis et al., 2012).
Since the clinical introduction of aprepitant, there have also been advances in the design of more potent and longer acting tachykinin NK 1 receptor antagonists (Reddy et al., 2006;Rojas et al., 2014). Netupitant is a novel orally active compound that penetrates into the brain and has a long duration of action and an insurmountable blocking activity at NK 1 receptors (Rizzi et al., 2012). Studies using NG108-15 cells have shown that netupitant and palonosetron have synergistic effects to antagonize substance P-induced calcium mobilization; synergism was not seen when netupitant was combined with ondansetron or granisetron (Stathis et al., 2012). Clinically, netupitant plus dexamethasone was shown to be superior to palonosetron plus dexamethasone against moderately emetogenic chemotherapy (Aapro et al., 2014), but the combination of netupitant and palonosetron with dexamethasone provided an excellent control of highly emetogenic chemotherapy induced-acute and delayed emesis, showing improvements over ondansetron and aprepitant combinations, with efficacy being maintained over multiple cycles (Gralla et al., 2014;Hesketh et al., 2014;Navari, 2015).
In the present studies, we used the ferret, a species with proven translational value in anti-emetic research (Percie du Sert et al., 2011), to explore the potential of a single administration of netupitant alone or in combination with palonosetron to inhibit cisplatin-induced acute and delayed emesis following an oral administration, compared with the control of emesis afforded by the three times per day administration of ondansetron alone, or when ondansetron was used daily combined with aprepitant and dexamethasone (Tattersall et al., 2000). An attempt was also made to characterize the spectrum of anti-emetic activity of netupitant to reduce emesis induced by other challenges. Apomorphine and morphine were selected to induce emesis via the area postrema (Lau et al., 2005;Percie du Sert et al., 2009), and intragastric copper sulfate was chosen to induce emesis via peripheral vagal and splanchnic pathways from the gastrointestinal tract (Kan et al., 2006). Ipecacuanha was selected as an emetogen inducing emesis via mixed central and peripheral pathways (Ariumi et al., 2000;Hasegawa et al., 2002). We also utilized Suncus murinus (house musk shrew) to investigate the potential of netupitant to reduce provocative motion-induced emesis that involves central pathways via vestibular inputs (Ueno et al., 1988;Ito et al., 2003). The studies described represent a reference point for others to compare the anti-emetic activity of new chemical entities or therapeutic strategies for the treatment of emesis.

Animals
Male castrated ferrets (0.8-1.8 kg) were obtained from the University of Leeds (England) or Southland Ferrets (Invercargill, New Zealand). Water and food (SDS Diet 'C, ' Special Diet Services, Ltd., UK, or TriPro super premium chicken meal formula dog food, American Nutrition, USA) were given ad libitum unless otherwise stated. Male S. murinus (45-65 g) were obtained from the University of Bradford (Bradford, U.K.). Dry pelleted trout pellets (Aquatic 3, Special Diet Services, UK) and water were given ad libitum unless otherwise stated. Animals were housed in temperature-controlled rooms at 20-24 ± 1 • C under artificial lighting, with lights on between 06:00 and 18:00 h. The relative humidity was maintained at 50 ± 5%. All experiments were conducted under license from the respective Governments of England, Switzerland and Hong Kong, and were approved by the relevant Animal Experimentation Ethics committees.

Determination of the Plasma Half-Life of Netupitant in Ferrets
Ferrets were anesthetized with halothane (3%) in oxygen and the left jugular vein was cannulated and exteriorized to the back of the neck using standard surgical techniques (Barnes et al., 1991). Animals were then allowed to recover for 2 weeks. On the day of the experiment, at t = 0, a 1.5 ml blood sample was withdrawn before administering netupitant (3 mg/kg, p.o.). Subsequently, further blood sampling (1.5 ml) was continued at 1, 3, 6, 12, 24, 36, 48, 60, 72, 84, and 96 h. Each sample was centrifuged at 10,000 g for 10 min and the plasma was stored at −80 • C prior to analysis. The analytical method used to determine the plasma concentration of netupitant was essentially the same as described by Huskey et al. (2003).

Experiments in Ferrets Involving
Apomorphine, Morphine, Ipecacuanha, and Cisplatin during Acute Observation Times On the day of experiment, the animals were transferred to individual cages where they were allowed at least 30 min to adapt before being presented with approximately 100 g of food. Netupitant (0.03-3 mg/kg) or vehicle [0.3% (v/v) Tween 80 in saline; 2 ml/kg] was then administered orally 2 h before the subcutaneous administration of apomorphine (0.125 mg/kg, s.c.), morphine (0.5 mg/kg, s.c.), ipecacuanha (1.2 mg/kg, p.o.), copper sulfate (100 mg/kg, i.g.), or cisplatin (10 mg/kg, i.p.). Animal behavior was recorded for up to 8 h using a closed circuit video recording system.

Experiments Involving Suncus murinus and Provocative Motion
The protocol used for the provocative motion-induced emesis experiments have been described previously (Chan et al., 2007). Briefly, the animals were transferred to the behavioral laboratory and given 30 min to habituate before being administered netupitant (0.03-3 mg/kg) or vehicle [0.3% (v/v) Tween 80 in saline; 2 ml/kg] orally. After 105 min, they were transferred to individual chambers (21 cm × 14 cm × 13 cm) and given 15 min to adapt before starting the provocative motion stimulus (i.e., 120 min post netupitant/vehicle administration; 4 cm horizontal displacement, 2 Hz, using a Heidolph Promax 2020 desktop shaker, Labplant, England). Animal behavior was recorded for 10 min using a closed circuit video recording system (Chan et al., 2007).

Experiments to Assess Anti-Emetic Potency on Cisplatin-Induced Acute and Delayed Emesis
The ferret acute and delayed emesis model has been described previously . Animals were transferred to individual observation cages where they were allowed at least 48 h to adapt. A dry pellet diet (Laboratory Feline Diet 5003, PMI Nutrition Inc., St. Louis, MO, USA) and water was available ad libitum. Animals were presented with 100 g of commercially available cat food (Whiskas R , Effem Foods Pty. Ltd., Wodonga, Australia) 30 min prior to drug/vehicle administration. In an initial series of experiments, ferrets were administered with cisplatin (5 mg/kg) intraperitoneally followed immediately by the oral administration of vehicle [0.3% (v/v) Tween 80 in saline; 2 ml/kg], netupitant (1-3 mg/kg), or ondansetron (1 mg/kg); the administration of vehicle or ondansetron was repeated at 8 h intervals.

Measurement of Emesis
Emesis was characterized by rhythmic abdominal contractions that were either associated with the forceful oral expulsion of solid or liquid material from the gastrointestinal tract (i.e., vomiting), or not associated with the passage of material Netupitant (3 mg/kg) or vehicle, was administered orally 2 h prior to challenge with emetic drugs. Data represents the mean ± SEM of the number of retches + vomits recorded during a 30-60 min observation period. Significant differences relative to the vehicle control treated animals are indicated as * * P < 0.01 (Student's unpaired t-test; n = 6).
(i.e., retching movements). For ferrets, consecutive episodes of retching and/or vomiting were considered separate when the animal changed its location in the observation cage, or when the interval between episodes exceeded 5 s . For S. murinus, episodes of emesis were also characterized as described above, except that the interval to demarcate two consecutive episodes of retching and/or vomiting was 2 s (Rudd et al., 1999). At the end of the observation period, animals were terminated by an intraperitoneal injection of pentobarbitone sodium (80 mg/kg).

Data Analysis
Data are expressed as the mean ± SEM, unless otherwise stated. Half-life values and ID 50 values were calculated from data expressed as a percentage of the control response using linear and non-linear regression analysis, respectively. In all cases, differences between treatment groups were considered significant when P < 0.05 (Student's T-test, or one way ANOVA and Tukey's multiple comparison tests, as appropriate; GraphPad Prism version 5.0, Inc. Version, San Diego, CA, USA).

Drug Formulation
Ondansetron hydrochloride d-hydrate, dexamethasone 21-phosphate disodium salt, and D-mannitol were from Sigma-Aldrich, St. Louis, MO, USA. Cisplatin (1 mg/ml in 0.1% mannitol in saline) was from David Bull Laboratories, Mulgrave, VIC, Australia. Palonosetron hydrochloride, aprepitant and netupitant were from Helsinn Advanced Synthesis SA, Switzerland. Ondansetron, dexamethasone, and palonosetron were dissolved in distilled water. Netupitant was dissolved in 0.3% Tween 80 in Saline (0.9% w/v). Aprepitant was dissolved in a solution of ethanol:propylene glycol:distilled water in the ratio of 1:6:3. Doses are expressed as the free base and dosing volumes were 1 ml/kg.

Pharmacokinetic Profile of Netupitant in Ferrets
The plasma level of netupitant peaked 1-3 h following its oral administration; the T max was 2.5 ± 0.5 h and the C max was 397.3 ± 19.7 ng/ml (n = 4). The plasma level of netupitant was measurable up to 96 h and had a flat terminal profile with a terminal half-life of 78.5 ± 17.0 h (n = 4).

Anti-Emetic of Activity of Netupitant against Drug-and Motion-Induced Emesis in the Ferret
No retching or vomiting was observed during the habituation periods and there were no obvious differences in the behavior of the animals randomized to the treatment groups prior to drug/vehicle administration. Netupitant was not associated with emesis during the pre-treatment period. Apomorphine, morphine and copper sulfate subsequently induced emesis in the vehicle treated animals following a short latency of ∼ 5 min (data not shown). The range of retches + vomits recorded during the 30 min observation periods were: apomorphine, 21-35; morphine, 33-55; and copper sulfate, 60-100. The oral administration of netupitant at 3 mg/kg completely prevented the retching and vomiting response to these challenges (P < 0.01; Table 1). A more detailed dose-ranging experiment against apomorphine (control retches + vomits range: 17-32) revealed that a significant 33.8% reduction of retching + vomiting could be observed at doses of netupitant as low as 0.03 mg/kg (P < 0.05); increasing the doses to 0.1 mg/kg reduced emesis by 75.6% (P < 0.01) and 0.3 mg/kg prevented emesis completely (P < 0.01; Figure 1). The ID 50 to inhibit apomorphine-induced retching + vomiting was 0.08 mg/kg, p.o. In the experiments involving ipecacuanha, the vehicle treated control animals exhibited emesis following a latency period of approximately 20 min (data not shown) that comprised ∼38 retches + vomits during the 60 min observation period. The emetic response induced by ipecacuanha was similarly prevented completely by netupitant at 3 mg/kg, p.o. (P < 0.05; Table 1). No dose-ranging experiments were done against ipecacuanha, copper sulfate or morphine, so ID 50 values could not be calculated.
(P < 0.05) and reduced the delayed response significantly by 94.6% (P < 0.01; Figure 3). In fact, the single administration of netupitant was almost twice as effective as the three times administration of ondansetron to prevent the acute and delayed retching and vomiting response (P < 0.01; Figure 3).
(1 mg/kg, i.p.) were highly effective to reduce the acute response significantly by 99.7 (P < 0.01) and 97.3% (P < 0.01), respectively (Figure 4). The standard regimen of ondansetron plus aprepitant and dexamethasone also reduced significantly the delayed emetic response by 86.3% (P < 0.01). The netupitant plus palonosetron and dexamethasone regimen reduced delayed emesis to a lesser extent by 60.2% (P < 0.05). However, there were no significant differences in the control of acute or delayed emesis by the two anti-emetic regimens (P > 0.05).

DISCUSSION
Netupitant was orally active to antagonize emesis induced by diverse emetogenic stimuli in ferrets and S. murinus. This profile is consistent with other NK 1 receptor antagonists that are presumed to be capable of penetrating the blood brain barrier to reach sites in the dorsal vagal complex and/or sites thought to be adjacent to the semi-compact part of the nucleus ambiguous (Tattersall et al., 1996;Fukuda et al., 1999;Andrews and Rudd, 2004). This is particularly relevant for centrally acting emetogens, such as apomorphine and morphine, or motion, but for other stimuli that may also cause a release of substance P in the periphery, the mechanism may in part also involve NK 1 receptors on the vagus or perhaps those located elsewhere in the periphery (Minami et al., 1998;Darmani et al., 2008;Ray et al., 2009).
A remarkably similar relative potency difference of other NK 1 receptor antagonists in the ferret cisplatin-induced emesis assay and the gerbil foot-tapping assay has been reported following intravenously administered brain penetrating NK 1 receptor antagonists, GR203040, CP-99,994, and L-742,694 (ID 50 values in foot taping assay ∼0.85 mg/kg; ID 50 values in the cisplatin assay were ∼0.18 mg/kg; Rupniak et al., 1997). The consistency of relative potency of the antagonists between studies may reflect netupitant's excellent bioavailability following oral administration (present investigations). It is also relevant that netupitant binds with high affinity to human NK 1 receptors (pKi = 9.0), with 1,000 fold selectivity over other tachykinin and G-protein coupled receptors (Rizzi et al., 2012). Predictably, in our studies, the use of netupitant at 3 mg/kg, p.o., abolished morphine-and ipecacuanhainduced emesis; a similar spectrum of effects has been reported for several other NK 1 receptor antagonists against these emetic stimuli [for a review, see ].
We also explored the anti-emetic potential of netupitant in the ferret cisplatin (low-dose 5 mg/kg)-induced acute and delayed emesis model Rudd and Naylor, 1997;Sam et al., 2001). In the present studies, the single oral administration of netupitant at 3 mg/kg was shown to be far superior to the three times per day administration of ondansetron (1 mg/kg, i.p.) at antagonizing both the acute (netupitant abolished acute emesis; ondansetron only reduced acute emesis by ∼68%) and delayed response (netupitant reduced delayed emesis by ∼95%; ondansetron reduced delayed emesis by ∼49%) to cisplatin; the antagonism afforded by ondansetron is comparable to previously published data in this species Naylor, 1994, 1997;Singh et al., 1997;Watanabe et al., 2008). These data demonstrate that netupitant has an exceptionally long duration of action which may relate to its exceptionally long plasma half-life of ∼79 h (present studies), whereas the plasma half-life of ondansetron is reported to be ∼2.3 h (Minami et al., 1991). If we place this in perspective, the first NK 1 receptor antagonist tested in the ferret acute and delayed emesis model, CP-99,994, [IC 50 of 1.9 nM to displace [125I]-Bolton Hunter Substance P from ferret brain membranes (Watson et al., 1995)], had a short plasma half-life of ∼1.4 h [data from cat, (Lucot et al., 1997)], and even when administered every 8 h at 10 mg/kg, only reduced cisplatin-induced acute emesis by 34% and reduced delayed emesis by ∼87% ; this was the dose of CP-99,994 that was also shown to prevent apomorphine, morphine, ipecacuanha, cisplatin (high-dose)-induced emesis (Bountra et al., 1993;Tattersall et al., 1993;Zaman et al., 2000). Critically, aprepitant, which has an IC 50 value of 0.7 nM at ferret NK 1 receptors (0.1 nM for human NK 1 receptors), and plasma half-life of 10 h [ferret; (Huskey et al., 2003); plasma half-life in humans, 10-29 h; (Bubalo et al., 2012)], only produces a similar level of inhibition of acute and delayed emesis when orally administered at 16 mg/kg (Tattersall et al., 2000).
Palonosetron (RS 25259-197) was first described as a high affinity 5-HT 3 receptor antagonist [pKi ∼10; Wong et al., 1995)] and was subsequently shown to have a plasma half-life of ∼40 h in humans (Siddiqui and Scott, 2004;Stoltz et al., 2004). It has been reported to inhibit cisplatin-induced emesis (10 mg/kg, i.v.; 5 h test) in ferrets and dogs with ID 50 values of 0.003 and 0.008 mg/kg, respectively; the dose of 0.1 mg/kg, p.o., inhibited emesis by almost 100% in both species (Eglen et al., 1995). Indeed, palonosetron was revealed as being twice as potent as ondansetron to inhibit cisplatin-induced emesis in ferrets following oral administration (Eglen et al., 1995). Clinically, palonosetron is recognized as being superior to other 5-HT 3 receptor antagonists, particularly against chemotherapyinduced delayed emesis (Navari, 2009;Saito et al., 2009). In fact, the magnitude of difference, particularly in the control of delayed emesis, was unexpected; prompting speculation that palonosetron has properties distinct from other selective 5-HT 3 receptor antagonists. There is strong evidence that it may have a different mechanism of action to ondansetron at the 5-HT 3 receptor, including an ability to cause receptor internalization, with continued binding to the internalized receptor to prolong the inhibition of its function (not seen with ondansetron and granisetron) and, more recently, an indirect inhibition of substance P responses, in vivo (Rojas et al., 2008(Rojas et al., , 2010a. In the cisplatin (5 mg/kg, i.p.) acute and delayed emesis model, we compared the anti-emetic potential of a regimen of netupitant and palonosetron (both administered before cisplatin) in combination with dexamethasone (administered before cisplatin and then every 24 h), with a regimen comprising ondansetron (administered before cisplatin and then every 24 h) and aprepitant (administered once before cisplatin) in combination with dexamethasone (administered before cisplatin and then every 24 h). Both treatment regimens were highly effective to antagonize acute emesis and were indistinguishable from each other. Indeed, the anti-emetic effect of the regimen of netupitant and palonosetron in combination with dexamethasone was still evident during the delayed phase of the response, and was not significantly different from the control of emesis seen following the more frequent dosing regimen of ondansetron and aprepitant in combination with dexamethasone. These data compare favorably with previous studies investigating aprepitant, ondansetron, and dexamethasone in ferrets (Tattersall et al., 2000).
Palonosetron administered as a single treatment, is not noted to have a U-shaped dose-response curve in ferrets or man against chemotherapy-induced emesis. However, in the least shrew, palonosetron appears to have a species-specific biphasic antiemetic action against the initial 2 h of emesis induced by cisplatin (10 mg/kg, i.p.), with a significant reduction at 0.1 and 2.5 mg/kg, s.c; while at 0.5 and 5 mg/kg the protection was lost (Darmani et al., 2014). In a subsequent set of experiments, palonosetron was tested at 0.1 mg/kg, s.c., against the same dose of cisplatin but over an extended observation period of 40 h (Darmani et al., 2015). Palonosetron reduced the emesis occurring during the initial first 16 h of the experiment by 83% compared to vehicle pretreated cisplatin controls. An assessment of emesis during the 27-40 h period also recorded a non-significant reduction of 73% (Darmani et al., 2015). Comparatively, netupitant at a high dose of 5 mg/kg, s.c., non-significantly reduced the emesis occurring during the first 16 h by 70% and abolished emesis during the 27-40 h period (Darmani et al., 2015). When both palonosetron and netupitant were combined together, the emesis occurring during the first 16 h was reduced by approximately 94%, and the reduction of emesis in the 27-40 h period was still significantly reduced (Darmani et al., 2015). Thus, the combination of palonosetron and netupitant resulted in a greater level of protection against both acute and 'delayed' phases of cisplatin-induced emesis in the least shrew than either treatment administered alone.
Suncus murinus is a more commonly used shrew species to study mechanisms of emesis, but its tachykinin receptor has a distinct pharmacology compared to the human and rodent NK 1 receptor (Tattersall et al., 1995;Rudd et al., 1999). However, in the present studies, netupitant dose-dependently reduced motion-induced emesis, with a dose of 0.3 mg/kg, p.o., preventing the response completely. In comparison with data in the literature, netupitant appears more potent than other NK 1 receptor antagonists including CP-99,994, GR203040, GR205171, and RP67580 to antagonize motion-induced emesis in this species (Gardner et al., 1995(Gardner et al., , 1996Rudd et al., 1999). It is known that patients with a history of motion sickness also have a higher incidence of chemotherapy-induced emesis (e.g., Morrow, 1984;Shih et al., 2009), post-operative nausea and vomiting, and pregnancy sickness (Bouganim et al., 2012;Warr, 2014). Therefore, it may be expected that netupitant would be useful to control emesis in such patients, where pathways involving motion sickness have been stimulated or perturbed.

CONCLUSION
The present studies revealed that netupitant has a broad inhibitory profile to inhibit emesis both in ferrets and S. murinus. In particular, a single dose of netupitant at 3 mg/kg given 3 h prior to the administration of cisplatin, provided almost complete protection from acute and delayed emesis; a lower dose of 1 mg/kg, in combination with a single oral dose of palonosetron at 0.1 mg/kg, in combination with daily administrations of dexamethasone (1 mg/kg, i.p.), was also highly effective to reduce acute and delayed emesis, being relatively comparable to a more frequent dosing regimen of ondansetron plus aprepitant in combination with dexamethasone. The convenience of oral dosing, efficacy, and long duration of action are consistent with clinical data. This has been realized by the successful formulation and use of palonosetron plus netupitant in a single pill (Akynzeo R ) for the treatment of chemotherapy-induced acute and delayed nausea and emesis (Thompson, 2014;Lorusso et al., 2015;Navari, 2015).

AUTHOR CONTRIBUTIONS
JR, GH, CG, EL, and CP conceived and designed the experiments. JR, GH, MN, and ZL performed the experiments and data analysis. All authors contributed equally to writing the manuscript.

FUNDING
This work was supported in part by Helsinn SA grant. Other support was from the Emesis Research Group, the Chinese University of Hong Kong.