Psoralea corylifolia L. Attenuates Nonalcoholic Steatohepatitis in Juvenile Mouse

Psoralea corylifolia L. (PC) is a traditional Chinese herb used to treat yang deficiency of the spleen and kidney in pediatric disease. Recent studies have shown its liver protection and anti-oxidative effects. The aim of this study was to explore the effect and mechanism of PC on nonalcoholic steatohepatitis in juvenile mice. The juvenile mouse model of nonalcoholic fatty liver disease/nonalcoholic steatohepatitis (NAFLD/NASH) was established by being fed a high-fat diet in maternal-offspring manner. PC granules were prepared and the quality was assessed. The main components were identified by high performance liquid chromatography. Then, different dosages of PC were administered for 6 weeks. Homeostatic model assessment of insulin resistance, plasma liver enzymes, hepatic morphology, hepatic superoxide anion, and triglyceride/total cholesterol levels were examined. The changes of nuclear factor-κB (NF-κB) activity phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt) and protein kinase C-α (PKC-α)/nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase signaling pathways in hepatic tissues were also determined. Our data demonstrated that PC significantly improved liver dysfunction, liver triglyceride/total cholesterol accumulation and insulin resistance in juvenile NAFLD/NASH mice. PC also alleviated hepatic steatosis, inflammatory cell infiltration, and fibroplasia in the portal area. Additionally, PC inhibited the activation of NF-κB and the mRNA expression of inflammatory factors while enhancing PI3K/Akt signaling in hepatic tissues. PC could also reduce hepatic superoxide anion levels, and NADPH oxidase activity as well as p47phox protein expression and PKCα activation in hepatic tissues. The results suggest that PC is effective in the treatment of NASH in juvenile mice. The mechanism may be related to the attenuation of hepatic oxidative stress through the PKC-α/NADPH oxidase signaling pathway.


INTRODUCTION
Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease worldwide. NAFLD includes a broad spectrum of liver disease, ranging from simple steatosis to nonalcoholic steatohepatitis (NASH) and NASH can progress to liver cirrhosis and hepatocellular carcinoma. The epidemic of NAFLD is rapidly becoming a severe public health problem (Watanabe et al., 2015;Younossi et al., 2016). Recently, NAFLD has been recognized in children and is a major cause for referral to pediatric gastroenterologists and hepatologists (Loomba et al., 2009;Mencin and Lavine, 2011;Anderson et al., 2015). Because pediatric NAFLD is closely related to metabolic syndrome and adult cirrhosis its epidemic attracts much attention (Alkhouri and Feldstein, 2012). In China, the prevalence of childhood obesity is also a major public concern (Ji, 2008;Guo et al., 2013). This may be attributed to the rise of middle-class families and the comprehensive opening of the "two-child" policy with the process of urbanization in China. It is reported that 68.2% of children who are obese exhibit a fatty liver (Fan, 2013;Zhu and Fan, 2013). Pediatric NAFLD has taken the place of chronic viral hepatitis to be a major health problem in China.
Unfortunately, to date, there are no approved therapies for children and adolescents with NAFLD other than lifestyle advice on diet and exercise (Zelber-Sagi et al., 2016;Fleet et al., 2017). However, several promising targets have been identified with the extensive investigation of the pathophysiological mechanism of NAFLD. Among these, the importance of oxidative stress (OS) in the pathogenesis of NAFLD has been well-documented (Spahis et al., 2017). Nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase is a key enzyme which mediated specific OS in the development of NAFLD. NADPH derived reactive oxygen species (ROS) that not only induces oxidative damage but also serves as a second messenger leading to inflammation and insulin resistance (IR) (Paik et al., 2014). Therefore, NADPH oxidase specific antioxidant therapies may be promising in the treatment of NAFLD.
Psoralea corylifolia L. (PC), a traditional Chinese herb, is widely used to treat yang deficiency of the spleen and kidney in pediatric disease in China. Modern pharmacological studies have proved that PC exerts beneficial effects on adult diseases such as cancer and osteoporosis (Chopra et al., 2013;Seo et al., 2014). In a recent study, its liver protection and anti-oxidative effects have also attracted attention (Seo et al., 2016). Our previous studies have found that PC can alleviate the kidney OS of rats with diabetic nephropathy, and its mechanism is related to the inhibition of NADPH oxidase (Zhou et al., 2013). However, the effect of PC on pediatric NASH is rarely reported. Therefore, the aim of this study was to explore the effect of PC on pediatric NASH in juvenile NAFLD mouse and investigate its possible mechanisms.

Animals
Male (n = 5) and female (n = 15) C57BL6J mice, age 56 to 63 days, obtained from the Institute of Laboratory Animal Sciences, CAMS&PUMC, were used in this study (Certification: no.11401300045242

Herbal Materials and Assessment of the Quality
Psoralea corylifolia L. concentrated granules were provided by Sanjiu Medical and Pharmaceutical Co. Ltd. (Shenzhen, China). One gram of PC granule equals to 20 g PC traditional Chinese herbals. High performance liquid chromatography (HPLC) was performed to identify the main chemical constituents in PC concentrated granules to assess the quality. Psoralen (P.N. 110739-201416) and Isopsoralen (P.N. 110738-201614) purchased from National Institutes for Food and Drug Control (Beijing, China), were taken as reference standards. HPLC was performed on Agilent 1260 system (USA) equipped with an Agilent ZORBAX Eclipse Plus C18 (250 × 4.6 mm, 5 µm). The mobile phase included methyl alcohol-waters (55:45, v/v) at a flow speed of 1.0 ml/min in condition of column temperature 30 • C. The detection wavelength was 246 nm.

Animal Modeling and Grouping
After adaptive feeding with a standard diet for 3 days, all female mice were randomized into the normal control group (n = 3) and model group (n = 12). All male mice were in the same randomization, with a ratio of 1:3 to female mice for mating. All male mice and female mice in the control group continued the standard diet. Female mice in four model groups were fed with high-fat diets 1 week before conception and during gestation and lactation. Litter size was standardized to four pups to guarantee that no litter was nutritionally biased. After weaning, the female offspring mice in the control group were selected and fed a standard diet: the normal control group (Control, n = 6). The female offspring mice in the model groups were selected and assigned to high-fat diets, generating four other groups: the juvenile NAFLD model group (Model, n = 6), low-dose PC treated group (PC-L, n = 6), high-dose PC treated group (PC-H, n = 6), vitamin E (Zhejiang Medicine Co., Ltd., Xinchang Pharmaceutical Factory, Shaoxing, China) treated group (VitE, n = 6). After 3 days, mice in the aforementioned treatment groups were administered corresponding therapy, PC-L (1.125 mg/g/d), PC-H (2.25 mg/g/d), or VitE (0.01 mg/g/d), intragastrically to 9 weeks of age. Oral gavage was performed once a day between 9:00 and 11:00 a.m. Mice in the normal control and juvenile NAFLD model group were administered the same volume of distilled water. Doses were adjusted to the body weight recorded once every 5 days.

Sampling
All the offspring female mice were euthanized at the end of 9 weeks. Blood samples were obtained by orbital sinus puncture at the time of euthanization. The specimen serum was collected and stored at −20 • C for analysis after centrifuging at 3,000 revolutions per minute for 30 min at 4 • C. Livers were quickly removed by laparotomy and flushed with normal saline on ice. Samples from the left outside lobe of the liver were fixed in 4% paraformaldehyde solution for paraffin embedding. Samples from the left inside lobe of the liver were prepared for frozen sections. Samples from the right lobe and the caudate lobe of the liver were preserved at −80 • C for the analysis.

Calculation of Insulin Resistance Index
Fasting blood glucose was measured by the hexokinase method using a GLU Kit (Shanghai Mind Bioengineering Co., Ltd., Shanghai, China). Fasting insulin was determined by enzymelinked immunosorbent assay method using a Mouse Ins (Insulin) ELISA Kit (Elabscience Biotechnology Co. Ltd., Wuhan, China). Homeostatic model assessment of insulin resistance (HOMA-IR) was calculated according to the formula as follows: fasting blood glucose (mmol/L) × fasting insulin (mIU/L)/22.5.

Quantitative Real-Time Polymerase Chain Reaction Analysis
Hepatic total RNA was extracted from the liver tissue with TRIzol reagent in accordance with the manufacturer's instructions. First, RNA purity and concentration were tested using a nucleic acid/protein analyzer (Thermo, USA). Second, the extracted total RNA (1 µg) was reverse transcribed using the PrimeScript TM RRT Reagent Kit with gDNA Eraser (TaKaRa Company, Dalian, China) on a Mastercycler gradient PCR apparatus (Eppendorf Company, Germany). Next, the complementary DNA was kept at −20 • C before PCR amplification. Next, real-time polymerase chain reaction (PCR) analyses were performed in 48-well optical PCR plates using SYBR R Premix Ex Taq TM (TaKaRa Company, Dalian, China) on an Applied Biosystems StepOne Real-Time PCR System (Thermo, USA). Finally, 2 − CT was used for analyzing the data. Primer sequences are listed in Table 1.

Measurement of Hepatic Superoxide Anion Levels
Serving as an oxidant-sensitive probe, dihydroethidium (DHE) is widely used for the measurement of ROS. Ethidium and 2-hydroxyethidium, two products of DHE oxidation, bind to the nuclear DNA, thereby forming a strong red fluorescent complex (Zhou et al., 2013). Frozen sections of liver (6 µm) were incubated with DHE (5 mmol/L, Beyotime Institute of Biotechnology, Shanghai, China) in a dark container at 37 • C for 30 min (Zhou et al., 2013) and then observed under an inverted microscope (IX51, Olympus, Japan).

Measurement of Liver NADPH Activity
Liver homogenate was lyzed in mammal tissue protein extraction reagent. The extracted protein was then supplemented with a protease inhibitor cocktail and phenylmethylsulfonylfluoride (PMSF) (Sinopharm Chemical Reagent Co. Ltd., Shanghai, China). Next, the samples were centrifuged at 12,000 revolutions per minute for 15 min at 4 • C. The supernatant was collected to quantify the protein concentration using a bicinchoninic acid protein assay kit (ASPEN Biotechnology Co. Ltd., Wuhan, China). Liver NADPH activity was measured using an NADPH Activity Quantification Kit (Genmed Scientifics Inc., Shanghai, China).

Western Blot Analysis
Liver extracted proteins (40 µg) were mixed with sample buffer, boiled for 5 min, and subjected to 8 and 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis gel graded from maximum to minimum of protein molecular weight (120 volts, 60-90 min). Separated proteins on the gel were transferred to 0.45 µm polyvinylidene fluoride membranes. The membranes were then blocked with 5% fat-free dry milk in Tris buffered saline with Tween (TBST) or 0.5% bovine serum albumin at room temperature for 1 h, followed by incubation with antibodies ( Table 2) at 4 • C overnight. The next day, after washing with TBST three times, the membranes were incubated with HRP-goat anti-rabbit/mouse antibody (ASPEN Biotechnology Co. Ltd., Wuhan, China) diluted 1:10,000 at room temperature for 30 min.

Gene
Forward  After washing with TBST four times, immunoreactive proteins were detected using the chemoluminescence method (LiDE110, Canon, Japan). Finally, band densities were determined by AlphaEaseFC software and quantified as the ratio between ODvalue of the target band to that of GAPDH.

Statistical Analysis
All data were analyzed using SPSS19.0 statistical software. Statistical significance was assessed by one-way analysis of variance following Kolmogorov-Smirnov normality. With homogeneity of variances, significance between different groups was determined by least standard deviation (LSD) test; alternatively, the Games-Howell test was used. A probability of <0.05 was considered to be statistically significant.

HPLC Profile of PC Concentrated Granules
The major active chemical constituents of PC are coumarins, flavonoids, and meroterpenes (Chopra et al., 2013;Zhang et al., 2016). According to Chinese Pharmacopoeia for the criterion of PC, coumarins: psoralea and isopsoralen were sleceted as markers for assessing the quality of the herb and some apropos preparations. HPLC of PC granules from three batches were shown in Figure 1. The result showed that the two main peaks were detected and identified by comparing the retention time with reference standards. Psoralea assay in three batches of PC PC Attenuated the Progression of NASH in Juvenile Mice

Plasma Liver Enzymes Levels
As shown in Table 3, untreated juvenile mice models presented with severe liver dysfunction. Both serum ALT and AST levels increased significantly in comparison with those of control mice (p < 0.01). However, treatment with different dose of PC and VitE reversed the increase in liver enzymes levels (p < 0.05, p < 0.01). Furthermore, a significant difference in serum ALT and AST levels was identified between high and low doses of PC, indicating a better improvement in liver dysfunction (p < 0.01).

Liver TG and TC Content
Compared with the juvenile mice in the control group, model mice presented with markedly higher liver TG and TC contents (p < 0.01). Mice in the PC-H and VitE groups exerted lower liver TG contents when compared with the model group (p < 0.01, p < 0.05). Mice in all treatment groups exerted lower liver TC contents when compared with the model group (p < 0.01). A significant difference in liver TC content was also identified between high and low dose of PC (p < 0.01). PC-H showed better effect on decreasing liver TG and TC contents than PC-L ( Table 3).

Homeostatic Model Assessment of Insulin Resistance
Mice in the model group showed severe IR characterized by elevated HOMA-IR (p < 0.01). However, treatment with PC and VitE significantly decreased HOMA-IR compared with that of untreated model mice (p < 0.01). Furthermore, there is a significant difference in HOMA-IR between high and low doses of PC, indicating that PC-H exerted better effect on improving IR than PC-L (p < 0.01; Table 3).

Histological Analysis
As shown in Figure 2, H&E-stained liver tissues of mice in model groups showed hepatocyte steatosis, swelling, and degeneration; and fibroplasia and inflammation in the portal area with lymphocytic infiltrate. However, compared with the model group, the pathological changes of the liver in each treatment group were improved, and the high-dose PC group were the lightest. In addition, the severity of the NAFLD in the juvenile mice livers was assessed using the Kleiner scoring system (Kleiner et al., 2005). As shown in Table 4, the Kleiner score of mice in the control group was zero. Model mice achieved the highest score of 6.33, which indicates NASH with evidence of severe steatosis, ballooned hepatocytes, and inflammation. After the treatment, PC-H mice achieved the lowest score of 2, which indicates mild NAFLD. Both PC-L and VitE treatment offspring presented with moderate NAFLD, as indicated by scores of 4.33 and 3, respectively.

PC Improved Liver Fibrosis in Juvenile Mice
To verify liver fibrosis, we observed the collagen deposition in the portal and perisinusoidal areas by sirius red-stained liver sections (Figure 3). For the mice in the model groups, collagen deposition mainly occurred in the portal area of liver tissues. It manifested as a bright yellow light on polarizing microscopy, indicating portal fibrosis with type I collagen deposition. For the mice in the treatment groups, the type I collagen also deposited in the portal area of liver tissues. However, treatment with different dose of PC and VitE significantly weakened the level of yellow color in liver tissues of juvenile mice. In addition, the PC-H group presented weaker light than that of the PC-L groups.

PC Inhibited the Activation of Liver Nuclear
Factor-κB in Juvenile Mice with NASH

Hepatic NF-κB Activity
Hepatic NF-κB activity was shown as the ratio of phosphorylated nuclear factor-κB (NF-κB) p65 to NF-κB p65 (p-p65/p65) protein expression in liver tissues. As shown in Figure 5, it exhibited a marked elevation in p-p65/p65 ration in    (Kleiner et al., 2005). The average score for each histological characteristic in each group was used. liver tissue of model mice (p < 0.01). The ratio was reduced after the treatment of PC or VitE (p < 0.05). Moreover, a reduction of p-p65/p65 was identified after high dose PC intervention when compared with low-dose PC (p < 0.05).

Hepatic PI3K p85 Expression
As shown in Figure 6, the expression of hepatic PI3K p85 protein level significantly was increased in model mice compared with that in control mice (p < 0.01). However, there was a significant reduction in the expression of PI3K p85 protein in all the treatment groups (p < 0.01). Additionally, PC-H treatment, better than PC-L treatment, remarkably decreased PI3K p85 protein expression (p < 0.01).

Hepatic Akt Activity
Hepatic Akt activity was shown as the ratio of phosphorylated Akt to Akt (p-Akt/Akt) protein expression in liver tissues. As shown in Figure 7, it exhibited a marked elevation in p-Akt/Akt (p < 0.01) in liver tissues of model mice. The ratio was significantly reduced after the treatment of PC or VitE (p < 0.01). Moreover, a significant reduction of p-Akt/Akt was identified after high-dose PC intervention when compared with low-dose PC (p < 0.01).

Hepatic Superoxide Anion Production
As shown in Figure 8, compared with the control mice, a significant high level of DHE fluorescence was observed in hepatic tissues of model juvenile mice (p < 0.01), indicating increased superoxide anion production. However, treatment with PC and VitE reduced the level of DHE fluorescence in liver tissues of juvenile mice (p < 0.01, p < 0.05). A high dose of PC treatment, compared with a low dose of PC, presented a greater decrease in peroxide anion production in liver tissues of juvenile mice (p < 0.01).

Hepatic NADPH Activity
As shown in Figure 9, the activity of hepatic NADPH was much higher in juvenile model mice than that in control mice (p < 0.01). After the treatment with PC and VitE, hepatic NADPH activity was significantly decreased (p < 0.01). Furthermore, a significant difference in hepatic NADPH activity was identified between different doses of PC, indicating a better hepatic NADPH decreasing activity of high doses of PC than that of low doses of PC (p < 0.01).

Hepatic p47 phox Protein Expression
As shown in Figure 10, hepatic p47 phox protein level was significantly increased in model mice compared with that in control mice (p < 0.01). However, there was a significant reduction in the expression of p47 phox protein in all the treatment groups (p < 0.01). Additionally, PC-H showed a better effect in decreasing p47 phox protein expression than PC-L treatment (p < 0.01).

Hepatic PKC-α Activity
Hepatic PKC-α activity was shown as the ratio of phosphorylated PKC-α to PKC-α (p-PKCα/PKCα) protein expression in liver tissues. As shown in Figure 11, it exhibited a marked elevation in p-PKCα/PKCα in liver tissue of model mice (p < 0.01). The ratio of p-PKCα/PKCα was significantly reduced after the treatment FIGURE 5 | The effect of PC on the p-p65/p65 ratio in hepatic tissues. Values are mean ± SD (n = 6). a p < 0.01 vs. the control group, b p < 0.05 vs. the model group, c p < 0.05 vs. PC-L group. p-p65: phosphorylated nuclear factor-κB p65; p65, nuclear factor-κB p65.  of PC or VitE (p < 0.01). Moreover, high-dose PC intervention showed a significant reduction of p-PKCα/PKCα when compared with low-dose PC (p < 0.01).

DISCUSSION
The traditional Chinese medicinal herb P. corylifolia L. (PC) is a hotspot in modern pharmacological research (Chopra et al., 2013). Thus far, the multiple pharmacological activities of PC have been discovered, especially its anti-osteoporosis and sex hormone-like effects (Li et al., 2014;Weng et al., 2015). However, the therapeutic effects of PC on metabolic diseases as fatty liver disease have rarely been reported and the mechanism is not clear. The NASH model of juvenile mouse can be successfully established with a maternal-offspring high-fat diet feeding method (Bruce et al., 2009;Williams et al., 2014;Zhou and Pan, 2015). And a gender difference in the pathology progress was reported: only female presents hepatic oxidative stress (Marin et al., 2016). Hence, in our study, the female model mice were chosen and sampled before body maturation (9 weeks; Tang and Kong, 2006). Before adulthood, the juvenile mice manifested the characteristics of NAFLD, ranging from liver dysfunction (ALT > AST), elevated liver TG and TC contents, and IR to distinct liver histological pattern, with the predominant damage in the portal area: increased portal inflammation and fibrosis (Schwimmer et al., 2005;Carter-Kent et al., 2009;Fleet et al., 2017). Further Kleiner score analysis confirmed that the phenotype of fatty liver in juvenile offspring model mice was NASH. PC could relieve liver dysfunction, IR, and the accumulation of liver TG and TC. With regard to liver morphology changes, PC also attenuated the histopathological abnormalities in hepatic tissue in juvenile mice. Moreover, a high dose of PC was better in the aforementioned effects than a low dose of PC. These results suggest that PC can improve NASH progression in juvenile mice and the therapeutic effect is possibly dose dependent.
We also evaluated liver fibrosis by H&E staining and sirius red staining. We observed fibroplasia in the portal area of model juvenile mice. The coloration proved that the main type of collagen deposition might be positively related to type I collagen.
However, the coloration level noticeably decreased after PC or VitE treatment. The high dose of PC showed the weakest light.
These results indicate that PC may be effective on ameliorating liver fibrosis process in NASH.
The pathogenesis of NAFLD involves OS, IR, and inflammation. Each of these elements is fueled by the other and each promotes the other, thereby forming a vicious circle (Tilg and Moschen, 2010). Moreover, in the development and progression of NAFLD/NASH, OS, the real "first hit, " appears as the most crucial pathological event during NAFLD development and the hallmark between simple steatosis and NASH manifestation (Spahis et al., 2017), contributing to the aggravation of liver fibrosis process (Ratziu et al., 2010). Hence, this study further investigates the mechanisms of PC from the aforementioned three aspects and pays more attention to probes into the OS situation.
First, we evaluated the changes of the NF-κB signaling pathway to elucidate the anti-inflammation mechanisms of PC. We found that NF-κB activity was significantly increased in liver tissue of juvenile model mice accompanied by the increase in the expression of NF-κB downstream inflammatory factors in liver tissue. These inflammatory factors involved inflammatory cytokines (TNF-α and IL-6) and chemokines (IL-8 and MCP-1). Treatment with PC decreased NF-κB activity and TNF-α, IL-6, IL-8, MCP-1 mRNA expressions. The effects of high dose were also superior to that of low dose. These results suggest that the protection mechanisms of PC against juvenile NASH may be related to reducing inflammation by inhibiting hepatic NF-κB signaling pathway.
Second, to explicate the mechanism of PC at attenuating IR, we estimated the changes of the hepatic PI3K-Akt signaling pathway. It is well known that the upregulation of the PI3K-Akt pathway is of benefit to IR improvement. Nevertheless, our study validated that the protein expression of PI3K p85 was significantly increased in liver tissue of juvenile model mice, accompanied by the increase in Akt activity in liver tissue. Treatment with PC decreased hepatic PI3K p85 protein expression and p-Akt/Akt. Interestingly, this paradox has also been reported in some studies (McKee et al., 2015;Pisonero-Vaquero et al., 2015;Bankoglu et al., 2016). Actually, it is noteworthy that the AKT, a major downstream target of the PI3K, affects lots of downstream signaling pathways which involvement of metabolism, cell growth, and cell survival (Sheppard et al., 2012;Matsuda et al., 2013). The PI3K-Akt signaling pathway strongly influences hepatic stellate cells proliferation, the main collagen-producing cells in liver, leading to hepatic fibrosis (Heinrichs et al., 2011). These results suggest that the protection mechanisms of PC against fibrosis rather than IR may be related to downregulate the hepatic PI3K-Akt signaling pathway.
Third, we investigated the changes of OS situation. Excessive ROS generated by OS induces oxidative damage by peroxidation of the biomacromolecule and interferes with hepatic cell signal transduction by serving as a second messenger (Brandes and Kreuzer, 2005). To calculate superoxide anion production, liver DHE staining was performed. The appearance of superoxide can change DHE into ethidium bromide, which binds to DNA and exhibits red fluorescence in the nucleus (Gracia-Sancho et al., 2008;Vazquez-Chantada et al., 2013). In our study, a massive increase in superoxide anion production was identified in the young model offspring mice. However, a high dose of PC treatment, compared with a low dose of PC and VitE, showed the maximum decreasing of superoxide anion production. These findings further confirm the beneficial effect of PC on improving OS in juvenile NASH.
It is widely believed that the mechanism of anti-oxidant activity in PC involves scavenging ROS and inhibiting ROS generation (Chopra et al., 2013;Zhang et al., 2016). Finally, FIGURE 9 | The effect of PC on the hepatic NADPH activity. Values are mean ± SD (n = 6). a p < 0.01 vs. the control group, b p < 0.01 vs. the model group, c p < 0.01 vs. PC-L group. NADPH, nicotinamide-adenine dinucleotide phosphate.
we assessed the changes of gene expression involved in the production of ROS. Among the multiple sources responding to chronic liver injury, ROS derived from NADPH oxidase is quite important to the development of NAFLD/NASH and hepatic fibrogenesis (Paik et al., 2014;Crosas-Molist and Fabregat, 2015). Our previous studies also confirmed that PC was effective in the treatment of diabetic nephropathy in rats and the underlying mechanism might be related to the attenuation of renal oxidative stress via PKC-α/NADPH oxidase signaling pathway (Zhou et al., 2013). Thus, the examination of the activity of NADPH oxidase and the expression of its upstream gene including PKC-α and p47 phox (Crosas-Molist and Fabregat, 2015) were carried out in the current study. We found that PC significantly inhibited NADPH oxidase activity and p47 phox protein expression in hepatic tissues of juvenile NASH mice. PKC-α activity was also decreased after PC treatment. PC at high dose was superior to PC at low dose in attaining the aforementioned outcomes. These results demonstrated that the inhibition of PC on ROS production might be related to the downregulation of PKC-α/NADPH oxidase signaling pathway in hepatic tissues.
In summary, we concluded that PC could improve the progression of NASH and ameliorate liver fibrosis in juvenile mice. The mechanism may be related to reduce inflammation by the NF-κB signaling pathway as well as inhibiting cells proliferation by the PI3K-Akt signaling pathway and decreasing OS through the PKC-α/NADPH oxidase signaling pathway. In addition, PC exhibits a dose dependent efficacy.
FIGURE 10 | The effect of PC on the expression of p47 phox in hepatic tissues. Values are mean ± SD (n = 6). a p < 0.01 vs. the control group, b p < 0.01 vs. the model group, c p < 0.01 vs. PC-L group.
FIGURE 11 | The effect of PC on the p-PKCα/PKCα ratio in hepatic tissues. Values are mean ± SD (n = 6). a p < 0.01 vs. the control group, b p < 0.01 vs. the model group, c p < 0.01 vs. PC-L group. p-PKCα, phosphorylated protein kinase C-α; PKCα, protein kinase C-α.