Impact Factor 4.400

The 1st most cited open-access journal in Pharmacology & Pharmacy

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Pharmacol. | doi: 10.3389/fphar.2018.00123

Icariin ameliorate thiram induced Tibial Dyschondroplasia via regulation of WNT4 and VEGF expression in broiler chickens

 Hui Zhang1*,  Khalid Mehmood1, Kun Li1, Mujeeb U. Rehman1, Xiong Jiang1, Shu-cheng Huang1, Lei Wang1, Lihong Zhang1, Xiaole Tong1,  Fazul Nabi1, Wangyuan Yao1, Muhammad K. Iqbal1, Muhammad Shahzad1 and  Jiakui Li1*
  • 1Huazhong Agricultural University, China

Tibal dyschondroplasia (TD) is main bone problem in fast growing poultry birds that effect proximal growth plate of tibia bone. TD is broadly defined as non-vascularized and non-mineralized, and enlarged GP with tibia bone deformation and lameness. Icariin (Epimedium sagittatum) is a traditional Chinese medicine, which is commonly practiced in the treatment of various bone diseases. Recently, many researcher reports about the beneficial effects of Icariin in relation to various types of bone conditions but no report is available about promoting effect of icariin against TD. Therefore, current study was conducted to explore the ameliorating effect of icariin in thiram induced TD chickens. A total 180 broiler chicks were equally distributed in three groups; Control, TD induced by thiram (50 mg/kg) and icariin group (treated with icariin @10 mg/kg). All groups were administered with normal standard diet ad libitum regularly until the end of experiment. The wingless type member 4 (WNT4) and vascular endothelial growth factor (VEGF) genes and proteins expression were analyzed by quantitative real-time polymerase chain reaction and western blot analysis respectively. Tibial bone parameters, physiological changes in serum, antioxidant enzymes and chicken growth performance were determined to assess advantage and protective effect of the medicine in broiler chicken. The expression of WNT4 was decreased while VEGF increased significantly (P < 0.05) in TD affected chicks. TD enhanced the growth plate, lameness and irregular chondrocytes, while reduced the liver function, antioxidant enzymes in liver and performance of chickens. Icariin treatment up-regulated WNT4 and down regulated VEGF gene and protein expressions significantly (P < 0.05), restored the growth plate width, increased growth performance, corrected liver functions and antioxidant enzymes levels in liver, and mitigated the lameness in broiler chickens. In conclusion, icariin administration recovered growth plate size, normalized performance and prevented lameness significantly. Therefore, icariin treatments are encouraged to reduce the incidence of TD in broiler chickens.

Keywords: Tibial dyschondroplasia, Icariin, Thiram, WNT4, VEGF

Received: 21 Jun 2017; Accepted: 05 Feb 2018.

Edited by:

Kok Gan Chan FASc, University of Malaya, Malaysia

Reviewed by:

SubbaRao V. Madhunapantula, Jagadguru Sri Shivarathreeswara University, India
Muhammad M. Ali, Afyon Kocatepe University, Turkey  

Copyright: © 2018 Zhang, Mehmood, Li, Rehman, Jiang, Huang, Wang, Zhang, Tong, Nabi, Yao, Iqbal, Shahzad and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Dr. Hui Zhang, Huazhong Agricultural University, Wuhan, China, 2371353498@qq.com
Prof. Jiakui Li, Huazhong Agricultural University, Wuhan, China, 842537868@qq.com