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Front. Pharmacol. | doi: 10.3389/fphar.2018.00131

Pharmacokinetic study of 7 compounds following oral administration of Fructus Aurantii to depressive rats

  • 1Xiangya Hospital Central South University, China
  • 2Nanjing University of Chinese Medicine, China
  • 3School of Medicine, Johns Hopkins University, United States

In the present study, the pharmacokinetics of multi-components (naringenin, nobiletin, meranzin hydrate, narirutin, naringin, hesperidin and neohesperidin) were investigated in acute depressive rats following oral administration of Fructus Aurantii (Zhi-Qiao, ZQ) extract (20 g/kg). A sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed to quantitatively or qualitatively analyze the 7 absorbed ingredients in the plasma, hippocampus and cortex of acute depressive rats. Biological samples were separated on a 300SB-C18 column, and the 7 compounds were detected with sequential positive and negative ionization modes. Our results confirmed that ZQ has antidepressant effects by decreasing the immobility time. In addition, this validated method showed good linearity (r ≥ 0.9987), and the lower limits of quantification were 2.73-16.38 ng/mL for the 7 analytes. This method successfully determined the pharmacokinetics of the 7 compounds and separated two pairs of isomers in plasma of acute depressive rats following oral administration of ZQ extracts. The 7 active ingredients were also identified as marked compounds in target tissues and should be further examined in pharmacokinetic studies with acute depressive rats. So, pharmacokinetic compounds were precisely linked with the antidepressant effect of ZQ in our study. This relationship is well-understood and contributes to the application of Traditional Chinese Medicine (TCM).

Keywords: Fructus aurantii, herb, pharmacokinetic, LC/MS/MS, Anti-depression

Received: 13 Aug 2017; Accepted: 06 Feb 2018.

Edited by:

Victor Kuete, University of Dschang, Cameroon

Reviewed by:

Germain S. Taiwe, University of Buea, Cameroon
Mikhail O. Nafiu, University of Ilorin, Nigeria
Souaibou Yaouba, University of Nairobi, Kenya  

Copyright: © 2018 Zhang, Zheng, Ren, Wang and Huang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Xi Huang, Nanjing University of Chinese Medicine, Nanjing, China,