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Front. Pharmacol. | doi: 10.3389/fphar.2018.00137

Shexiang Baoxin Pill corrects metabolic disorders in a rat model of metabolic syndrome by targeting mitochondria

 Dan Wei1, Ningning Zheng1, Lanyang Zheng2, Leting Wang3, Liang Song4 and  Luning Sun1*
  • 1Department of Pathophysiology, College of Basic Medical Science, China Medical University, China
  • 2Department of Pathogen Biology, College of Basic Medical Science, China Medical University, China
  • 3Shanghai Hutchison Pharmaceuticals, China
  • 4Wanleibio Co., Ltd, China

Metabolic syndrome (MS) is a global epidemic associated with great socioeconomic and public health impact. The objective of this study was to observe the effects of Shexiang Baoxin Pill (SBP) on a MS rat model and then investigate the underlying mechanisms. Diet-induced MS model was established according to the classic formula, then the MS rats were randomly divided into two groups: control group (0.9% NaCl, 100mg/kg•d) and SBP treated group (SBP, 100mg/kg•d). The systolic blood pressure (SBP), fasting blood glucose (FBS), triglycercide (TG), high-density lipoprotein cholesterol (HDL-C), body weight and abdominal perimeter were dynamically monitored and analyzed. Serum leptin, adiponectin, TNF-α, IL-6 and IL-10 levels were measured by ELISA. The expression of leptin, adiponectin, TNF-α, IL-6 and IL-10 in adipose tissue and the expression of AMP-activated protein kinase (AMPK) and Peroxisome proliferator-activated receptor gamma coactivator 1-α (PGC-1α) in heart, liver, skeletal muscle and adipose tissue were measured by Western Blot; The expression of the mitochondrial protein UCP2, Cytochrome b and ATPase was observed by immunofluorescent staining.SBP could significantly decrease serum TG, TC, LDL-C levels and increase HDL-C levels. SBP also optimized the ratio of leptin/adiponectin by decreasing the expression of leptin and increasing the expression of adiponectin in adipose tissue; SBP antagonized the inflammatory reactions by inhibiting the expression of TNF-α and IL-6, and stimulating the expression of IL-10 in adipose tissue; SBP improved the lipid metabolism by up-regulating the expression of AMPK and PGC-1α. Furthermore, SBP improved the metabolic disorder and complications of MS by adjusting the expression of several mitochondrial protein including UCP2, Cytochrome b and ATPase.SBP has prominent therapeutic effects on the metabolic syndrome, and the possible mechanisms might be related to its anti-inflammation, lipid metabolism improving, anti-oxidation and mitochondrial protective function.

Keywords: metabolic syndrome, Shexiang Baoxin Pill, Lipid Metabolism, Inflammation, Mitochondrial Proteins, Reactive Oxygen Species

Received: 23 Nov 2017; Accepted: 07 Feb 2018.

Edited by:

Steyner F. Cortes, Universidade Federal de Minas Gerais, Brazil

Reviewed by:

Roger L. Santos, Federal University of Espírito Santo, Brazil
Verónica Guarner-Lans, Instituto Nacional de Cardiología, Mexico  

Copyright: © 2018 Wei, Zheng, Zheng, Wang, Song and Sun. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Luning Sun, China Medical University, Department of Pathophysiology, College of Basic Medical Science, Shenyang, China, lnsun@cmu.edu.cn