Impact Factor 4.400

The 1st most cited open-access journal in Pharmacology & Pharmacy

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Pharmacol. | doi: 10.3389/fphar.2018.00674

Epigallocatechin-3-gallate promotes the growth of mink hair follicles through sonic hedgehog and AKT signaling pathways

Haihua Zhang1, 2, Weixiao Nan3, Shiyong Wang1, Xingchao Song1, Huazhe Si1, Tong Li4 and  Guangyu Li1*
  • 1State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences (CAAS), China
  • 2College of Animal Science and Technology, Hebei Normal University of Science and Technology, China
  • 3College of Animal Science and Technology, Jilin Agriculture University, China
  • 4High-tech Zone Laboratory of Public Test and Analysis Service, China

Background: Hair follicles play an essential role in the growth of hair. Epigallocatechin-3-gallate (EGCG), a catechin polyphenol in green tea, has various bioactivities. The present study aims to evaluate the effect of EGCG on the growth of mink hair follicles and investigate the possible molecular mechanisms.
Methods: The length of hair follicles was recorded up to 6 days in presence of 0.1-5 μM EGCG. Primary dermal papilla cells (DPCs) and outer root sheath cells (ORSCs) were treated with 0.25-4 μM EGCG, and their growth was evaluated by MTT assay and cell cycle detection. The levels of key molecules in sonic hedgehog (Shh) and AKT signaling pathways were further assessed by quantitative real-time PCR, western blot and immunofluorescence. To determine the involvement of Shh and AKT pathways in EGCG-mediated growth-promotion of ORSCs and DPCs, Shh pathway inhibitors cyclopamine and GANT61 or AKT pathway inhibitor LY294002 were employed, and then cell proliferation and cell cycle were analyzed.
Results: Data from ex vivo culture showed that, in presence of 0.5-2.5μM EGCG, the growth of mink hair follicles was promoted. In vitro, the proliferation of DPCs and ORSCs was enhanced by 0.5-4 μM EGCG treatment. More cells entered S phase upon treatment of EGCG, accompanied with upregulation of cyclin D1 and cyclin E1. Furthermore, when exposed to EGCG, the Shh and AKT signaling pathways were activated in both hair follicles and primary DPCs and ORSCs. Inhibiting either of these two pathways partly reversed the effect of EGCG on proliferation and cell cycle of DPCs and ORSCs.
Conclusion: EGCG promotes the growth of mink hair follicles at concentrations of 0.5-2.5μM. This growth-promoting effect of EGCG may be associated with the increased proliferation of DPCs and ORSCs through activating Shh and AKT signaling pathways.

Keywords: Dermal papilla cells, epigallocatechin-3-gallate, Hair follicle growth, Outer root sheath cells, Akt signaling pathway, Sonic hedgehog signaling pathway

Received: 13 Dec 2017; Accepted: 05 Jun 2018.

Edited by:

Wolff M. Kirsch, Loma Linda University Medical Center (LLUMC), United States

Reviewed by:

Lifeng Kang, University of Sydney, Australia
Andrzej T. Slominski, University of Alabama at Birmingham, United States  

Copyright: © 2018 Zhang, Nan, Wang, Song, Si, Li and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Guangyu Li, Institute of Special Animal and Plant Sciences (CAAS), State Key Laboratory of Special Economic Animal Molecular Biology, Changchun, China, li_guangyu123@sina.com