Spinacetin Suppresses the Mast Cell Activation and Passive Cutaneous Anaphylaxis in Mouse Model

We previously reported the anti-inflammatory and anti-asthmatic activities of the extract of the Inula japonica Thunb. Aiming for discovery of a novel anti-inflammatory compound, we isolated spinacetin from the extract and investigated its in vitro and in vivo anti-inflammatory effect and the related mechanism. Effect of spinacetin on the Syk signaling pathway was studied in bone marrow-derived mast cells (BMMCs), and that on the nuclear factor-κB (NF-κB) and mitogen-activated protein kinases (MAPKs) was investigated in Rat basophilic leukemia (RBL)-2H3 cells and human mast cell line (HMC-1). The in vivo anti-inflammatory activity was assessed with passive cutaneous anaphylaxis (PCA) reaction assay. Spinacetin significantly inhibited the release of histamine, and production of inflammatory mediators such as leukotriene C4 (LTC4) and interlukin-6 (IL-6) in IgE/Ag stimulated BMMCs. Analysis of the signaling pathways demonstrated that spinacetin inhibited activation of Syk, linker of activated T cells (LAT), phospholipase Cγ (PLCγ), cytosolic phospholipase A2 (cPLA2), MAPKs, Akt/NF-κB, and intracellular Ca2+ mobilization but with no effect on Fyn and Lyn. On the other hand, spinacetin suppressed IgE/Ag-induced activation of RBL-2H3 cells with inhibition against phosphorylation of extracellular signal regulated-protein kinase (ERK), c-Jun-NH2-terminal kinase (JNK), p38 MAPKs, PLCγ, translocation of cPLA2, and Akt/IκBα/NF-κB signal. However, spinacetin had no effect on PMA and A23187-induced activation of HMC-1. Furthermore, oral administration of spinacetin dose-dependently attenuated IgE/Ag-mediated PCA reaction in mouse model. Taken together, spinacetin showed the activities in preventing inflammatory processes, which might be at least partially attributed to the abolishment of Syk-dependent activation of IgE/Ag-mediated mast cells.


INTRODUCTION
Mast cell is known to contribute to allergic diseases such as asthma, rhinitis, urticaria, allergic conjunctivitis, and anaphylaxis (Modena et al., 2016). Mast cells are important effector cells of IgE-mediated immunological and allergic inflammatory reactions (Siraganian, 2003). The aggregation of high affinity IgE receptors (FcεRI) on mast cells via antigen (Ag) triggers the release of various preformed mediators (including histamine and proteases), newly synthesized lipids including LTs, prostaglandins (PGs), and many inflammatory cytokines such as IL-6, tumor necrosis factor (TNF)-α (Kopec et al., 2006). These mediators play pivotal roles in allergic and inflammatory reactions.
Spinacetin ( Figure 1A) is a flavonoid isolated from Inula japonica Thunb (Zhu et al., 2014). In our previous study, the extract of Inula japonica showed anti-inflammatory and anti-asthmatic activities (Park et al., 2011;Lu et al., 2012). It was reported that spinacetin reduces prostaglandin E 2 level in macrophages (Moscatelli et al., 2006), and our group found that spinacetin inhibits LTC 4 synthesis and degranulation in c-Kit ligand induced mast cells (Zhu et al., 2014). However, the anti-inflammatory effect of spinacetin on IgE/Ag-mediated mast cells and anaphylaxis has not been reported yet. Anaphylaxis is a severe systemic reaction closely related to mast cell activation (Akin, 2015). Therefore, we recently evaluated anti-allergic effect of spinacetin and its related molecular mechanism in mast cells and PCA models.

Plant Material
Spinacetin was isolated from the ethanol extract of the I. japonica (Supplementary Material). The plants of I. japonica were collected from Henan Province, China, and identified by Professor Y. Zhou (Department of Pharmacognosy, School of Pharmacy, Tianjin Medical University). A voucher specimen (IJ201105) was deposited at School of Pharmacy, Tianjin Medical University, China. The flower part of I. japonica was used to isolate spinacetin. Prior to use, spinacetin was dissolved in dimethyl sulfoxide (DMSO).

Cell Culture
Bone marrow-derived mast cells were isolated from bone marrow of Balb/c mice and differentiated as described by us previously (Jin et al., 2017). Briefly, bone marrow cells from Balb/c mice were cultured in RPMI1640 containing 0.1 mM non-essential amino acid solution, 100 U/ml of penicillin, 100 µg/ml of streptomycin, 10% FBS and 20% pokeweed mitogen-stimulated spleen conditioned medium as a source of IL-3. The differentiated cells were available for use after 3 weeks, when more than 99% were found to become BMMCs as checked with the method previously described (Murakami et al., 1994). HMC-1 was kindly provided by Dr. Joseph Butterfield (Mayo Clinic, Rochester, MN, United States) and RBL-2H3 cells was purchased from the Cell Bank of Chinese Academy of Sciences (Shanghai, China). HMC-1 and RBL-2H3 cells were cultured in IMDM (Iscove's modified Dulbecco's medium) and DMEM (Dulbecco's Modified Eagle's Medium) supplemented with 10% FBS without IL-3, respectively. (C) IgE-sensitized BMMCs were pre-treated with spinacetin or Bay 61-3606 for 1 h, and then stimulated with DNP-HSA for 15 min. The amount of histamine released into the culture media was measured using ELISA. (D) IgE-sensitized BMMCs were pre-incubated with FluoForte TM dye-loading solution for 1 h, then treated with spinacetin or Bay 61-3606 for 1 h. The fluorescence was measured after stimulation with DNP-HSA for 5 min. Bay 61-3606 was used as positive control. The data show the mean ± SEM of three independent experiments. * * P < 0.01 and * * * P < 0.01, compared with the cells with IgE/Ag stimulation but without spinacetin treatment.
Activation of BMMCs, RBL-2H3, and HMC-1 Bone marrow-derived mast cells and RBL-2H3 were sensitized with 500 ng/ml of anti-DNP IgE overnight, and then treated with spinacetin or Bay 61-3606 for 1 h. After stimulated with 100 ng/ml of DNP-HSA (Ag) for 15 min or 6 h, the supernatants were collected for further analysis, respectively. The levels of LTC 4 , histamine and IL-6 were determined using an immunoassay kit according to the manufacturer's protocol. The HMC-1 cells were incubated with spinacetin in the presence or absence of phorbol 12-myristate 13-acetate (PMA) and calcium ionophore (A23187).

Measurement of Intracellular Ca 2+ Level
Intracellular Ca 2+ level was determined with the FluoForte Calcium Assay Kit (Enzo Life Sciences, Ann Arbor, MI, United States) as described previously (Jin et al., 2017). Briefly, IgE-sensitized BMMCs were pre-incubated with FluoForte TM dye-loading solution for 1 h, and then treated with spinacetin or Bay 61-3606 for 1 h. After addition of DNP-HSA and incubation for 5 min, the fluorescence was monitored with a multilabel plate reader at Ex = 485 nm/Em 535 nm (Perkin Elmer's VICTOR TM X5 Multilabel Plate Reader, Waltham, MA, United States).

Extraction of Nuclear Proteins
Nuclear extracts were prepared as described before. Briefly, IgEsensitized BMMCs and RBL-2H3 cells were pretreated with spinacetin for 1 h, followed by incubation with DNP-HSA for 30 min. Then the nuclear proteins were prepared by using Nuclear Extraction Kit according to the manufacturer's protocol (Panomics, Fremont, CA, United States).

Western Blot Analysis
IgE-sensitized BMMCs or RBL-2H3 were pretreated with spinacetin or Bay 61-3606 for 1 h and stimulated with DNP-HSA. The preparation of cell lysates and Western blot were performed as described previously (Wang et al., 2015). To assess COX-2 expression, BMMCs were pre-incubated with 1 µg/ml aspirin for 2 h to irreversibly inactivate preexisting COX-1. In the case of HMC-1, cells were pretreated with spinacetin for 1 h, stimulated with PMA and A23187, then collected for preparation of the cell lysates.

Immunoprecipitation (IP)
Total cell lysates were incubated with anti-Syk, anti-LAT, anti-Fyn or anti-Lyn antibody overnight, and immune-complexes were precipitated with protein A/G plus agarose. Precipitates were subjected to SDS-PAGE and immunoblotted with the respective antibodies.

PCA
ICR mice (7 weeks old male) were intradermally injected with 80 ng of mouse anti-dinitrophenyl (DNP) IgE (Sigma) into one ear. After 24 h, spinacetin or dexamethasone (Dexa) was orally administered. One hour later, the mice were intravenously challenged with 60 µg of DNP-HSA in 200 µl of PBS containing 1% (w/v) Evans blue. The mice were euthanized 1 h after treatment with DNP-HSA. The ears were removed, and then dissolved with 400 µl of formamide at 63 • C overnight to measure the amount of dye extravasated by Ag. The absorbance of dye was measured at 630 nm using a microplate reader (BIO-RAD iMark, Hercules, CA, United States). The ears were fixed with 4% formaldehyde and embedded in paraffin. Four micrometer sections of the tissues were prepared and stained with toluidine blue to count the number of mast cells. All experiments using animals were approved on Oct 27, 2017, by the Institutional Animal Care and Use Committee of Tianjin Medical University (No. of allowance: TMUaMEC2017022).

Statistical Analysis
All values are expressed as means ± SEM of triplicate values. One-way ANOVA was utilized to determine the statistical significance with GraphPad Prism 7 (GraphPad Software, La Jolla, CA, United States). Differences were considered statistically significant when P < 0.05.

Spinacetin Suppresses Histamine Release and Ca 2+ Mobilization in IgE/Ag-Stimulated BMMCs
The MTT assay was employed to determine the cell viability of spinacetin against BMMCs. As shown in Figure 1B, spinacetin was not found to affect cell viability at concentration of less than 5 µM. Thus, concentrations of 1, 2, and 5 µM were chosen for further study to assess the anti-inflammatory mechanism.
FcεRI aggregation is known to stimulate mast cell release of preformed mediators, including histamine, protease, and proteoglycans (Kopec et al., 2006). And histamine is a crucial mediator in the inflammation and allergic response (Gibbs and Levi-Schaffer, 2012;Cataldi et al., 2014). Therefore, we investigated the inhibitory effect of spinacetin on histamine release in IgE/Ag-stimulated BMMCs. As shown in Figure 1C, spinacetin strongly inhibited release of histamine. Increased cytosolic Ca 2+ mobilization leads to mast cell degranulation and production of various pro-inflammatory mediators (Kopec et al., 2006). Thus, we examined the effect of spinacetin on intracellular Ca 2+ mobilization. As shown in Figure 1D, intracellular Ca 2+ level was potently increased in activated BMMCs, and this increase was attenuated by spinacetin. The Syk inhibitor Bay 61-3606 was used as positive control. These data indicate that spinacetin inhibits mast cell degranulation through suppression of Ca 2+ mobilization.

Spinacetin Inhibits LTC 4 Generation via Blocking MAPKs Phosphorylation and cPLA 2 Translocation Into the Nucleus
Leukotrienes are a family of potent eicosanoid lipid mediators with key role in the pathogenesis of several inflammatory diseases like asthma (Duroudier et al., 2009). When activated by IgE/Ag, mast cells mobilize arachidonic acid through cPLA 2 , and rapidly synthesize LTC 4 (Jin et al., 2017). As shown in Figure 2A, after spinacetin treatment, the generation of LTC 4 was significantly decreased in a dose-dependent manner in IgE/Ag-stimulated BMMCs. IgE/Ag stimulation potently increased cPLA 2 phosphorylation and promoted its nuclear translocation, while this increased activation was down-regulated by spinacetin (Figures 2B-D). The enzymatic activity of cPLA 2 is known to be increased by phosphorylation of MAPKs (Lin et al., 1993). Therefore, next we assessed the effect of spinacetin on phosphorylation of MAPKs. As shown in Figures 2E-H, the IgE/Ag-induced phosphorylation of ERK1/2, JNK1/2 and p38 was significantly decreased by spinacetin. The above results indicated that inhibition of ERK1/2, JNK1/2, and p38 by spinacetin was involved in blockade of cPLA 2 activation and the subsequent LTC 4 synthesis.

Spinacetin Decreases IL-6 and COX-2 Expression by Suppressing Akt/IκBα/NFκB Pathway
Interlukin-6 is a pleiotropic cytokine and plays an important role in inflammatory diseases (Yao et al., 2014). Therefore, we examined the effect of spinacetin on IL-6 production in IgE/Ag-stimulated BMMCs. Figure 3A shows that spinacetin treatment inhibited level of IL-6 dose dependently. COX-2 is an important enzyme regulating production of PGs, which are important mediators in inflammation (Tsatsanis et al., 2006). In order to abolish preexisting COX-1, BMMCs were pre-treated with aspirin for 2 h. Then the cells were stimulated with DNP-HSA for 7 h in the absence or presence of spinacetin. As shown in Figure 3B, the COX-2 protein was not detected in unstimulated BMMCs. Stimulation with IgE/Ag potently induced expression of COX-2, which was reduced by spinacetin in a dose-dependent manner.
Nuclear factor-κB positively regulates the production of IL-6 and COX-2 expression (Tsatsanis et al., 2006;Ponce et al., 2009). Activation of NF-κB, an essential transcription factor involved in the inflammatory responses, occurs following the phosphorylation, ubiquitination and degradation of IκBα. Then activated NF-κB translocates from cytosol into the nucleus (Chen et al., 1999). RelA (p65) is a subunit of NF-κB (Ghosh and Karin, 2002). In order to study whether spinacetin decreases the generation of IL-6 through inactivation of NF-κB or not, we investigated the effect of spinacetin on NF-κB. As shown in Figures 3C-E, p65 translocated from cytosol to the nucleus after stimulation with IgE/Ag. However, spinacetin significantly inhibited p65 translocation. Furthermore, spinacetin treatment suppressed phosphorylation and degradation of IκBα (Figures 3C,F,G). NF-κB is known to be activated by PI3K/Akt FIGURE 2 | Spinacetin suppresses LTC 4 generation, cPLA 2 translocation, and MAPKs phosphorylation. IgE-sensitized BMMCs were pre-incubated with indicated concentrations of spinacetin or Bay 61-3606 for 1 h, then stimulated with DNP-HSA for 15 min. (A) The culture medium was collected and measured for generation of LTC 4 . (B) Cytosolic and nuclear fractions were immunoblotted with antibody for phospho-cPLA 2 , and relative ratios of cytosolic p-cPLA 2 (C), nuclear p-cPLA 2 (D) proteins were determined by analyzing immunoblot band intensities. (E) Cell lysates were immunoblotted for the phosphorylated and total forms of ERK1/2, JNK1/2, and p38. Relative ratios of cytosolic p-ERK1/2/ERK1/2 (F), p-JNK1/2/JNK1/2 (G), and p-p38/p38 (H) proteins were determined by analyzing immunoblot band intensities. The results show the mean ± SEM of three independent experiments. * P < 0.05, * * P < 0.01, and * * * P < 0.001, compared with the cells with IgE/Ag stimulation but without spinacetin treatment.
FIGURE 3 | Spinacetin inhibits production of IL-6 and expression of COX-2 through down-regulation of Akt/IκB/NFκB pathway. (A) IgE-sensitized BMMCs were pre-treated with different concentrations of spinacetin for 1 h and stimulated with DNP-HSA for 6 h. The supernatants were collected to determine the levels of IL-6 by ELISA. (B) IgE-sensitized BMMCs were pretreated with aspirin for 2 h, treated with spinacetin for 1 h, and then stimulated with DNP-HSA for 7 h. The immunoblot was then probed with anti-COX-2 antibody. (C) IgE-sensitized BMMCs were pre-incubated with spinacetin for 1 h and then stimulated with DNP-HSA for an additional 15 min. Cell lysates of cytosolic and nuclear proteins, and total cell lysates were examined with western blot analysis for p65, p-IκBα, IκBα, and p-Akt. (D-H) Relative ratios of the respective proteins were determined by analyzing immunoblot band intensities. The results show the mean ± SEM of three independent experiments. * * P < 0.01 and * * * P < 0.001, compared with the cells with IgE/Ag stimulation but without spinacetin treatment.
pathway (Manukyan et al., 2010;Shi et al., 2016). IgE/Ag stimulation resulted in increase of the phosphorylated Akt, and spinacetin inhibited this activity (Figures 3C,H). Our results suggested that inhibition of Akt by spinacetin might be involved in suppression of IL-6 and COX-2 expression.
Spinacetin Inhibits RBL-2H3 Activation but Does Not Affect HMC-1 RBL-2H3 cells are widely used for in vitro studies of mast cellmediated allergic inflammation. Stimulation of RBL-2H3 with IgE/Ag increased ERK1/2, JNK1/2, and p38 phosphorylation as compared to non-treated cells (Figures 4A,D). Spinacetin reduced the activation of the MAPKs. Meanwhile, spinacetin markedly reduced translocation of the phosphorylated cPLA 2 from cytosol into the nucleus (Figures 4A,D). Moreover, spinacetin suppressed IκBα phosphorylation and degradation, and NF-κB activation, and also inhibited Akt which is known to activate IκBα and NF-κB (Figures 4B,E). These results are consistant with that obtained in IgE/Ag-stimulated BMMCs. Furthermore, the phosphorylation of PLCγ was inhibited by spinacetin (Figures 4C,F). These results demonstrate that spinacetin ameliorated the allergic reaction in IgE/Ag stimulated mast cells.
HMC-1 cells are characterized to lack expression of FcεRI. Therefore, many researchers use calcium ionophores and phorblo esters to measure mast cell activity by using HMC-1 cells. HMC-1 can be activated by PMA and A23187, and the activated HMC-1 leads to the phosphorylation of tyrosine kinase followed by the activation of MAPKs, NF-κB, and release of proinflammatory cytokines (Park et al., 2014). In our study, HMC-1 cells were pre-treated with spinacetin for 1 h, and then stimulated with PMA and A23187. However, spinacetin did not suppress phosphorylation of MAPKs, cPLA 2 , Akt and IKKα/β in HMC-1 cells (Figures 4G,H).
These results suggest that spinacetin only inhibits IgE/Aginduced mast cells activation, and that target protein of spinacetin might be upstream of Akt.

Spinacetin Inhibits PLCγ, LAT, and Syk Phosphorylation Independent of Lyn and Fyn
Lyn phosphorylates and activates Syk and LAT. Phosphorylation of LAT results in the recruitment of molecules such as Grb2, Gads, SOS, and PLCγ. These interactions with LAT lead to the formation of molecular signaling complex that is required for the release and generation of pro-inflammatory mediators in mast cells (Gilfillan and Tkaczyk, 2006). Fyn is also required for mast cell degranulation, cytokine, and chemokine production (Kopec et al., 2006). Fyn phosphorylates Gab2 to activate PI3K upon FcεRI aggregation (Rivera and Gilfillan, 2006). Activated PLCγ induces mobilization of cytosolic Ca 2+ and activates PKC, respectively (Kopec et al., 2006). As shown in Figure 5A, Ag stimulation increased phosphorylation of PLCγ, and spinacetin remarkably reduced phosphorylated PLCγ. Treatment with spinacetin inhibited the histamine release, LTC 4 synthesis and IL-6 production, which are critical for allergic inflammation. And generation of the pro-inflammatory mediators was controlled by Ca 2+ mobilization, cPLA 2 , MAPK, NF-κB, and PLCγ, respectively. Therefore, next we investigated whether spinacetin affects the upstream molecules such as LAT, Syk, Lyn, and Fyn. As shown in Figures 5B-D, spinacetin inhibited phosphorylation of LAT and Syk. However, spinacetin did not affect Lyn and Fyn (Figures 5E-G). These observations indicated that spinacetin inhibited the IgE-mediated mast cell activation through regulating Syk dependent pathways.

Spinacetin Attenuates IgE-Mediated PCA
The FcεRI/mast cell axis is involved in triggering several intracellular signaling molecules to release preformed granules, and to generate pro-inflammatory mediators, resulting in the induction of allergy and anaphylaxis (Manikandan et al., 2012). As we already found that spinacetin had shown potent antiinflammatory activity in IgE/Ag-stimulated mast cells, therefore we assessed the anti-allergic effects of spinacetin by using a PCA model in mice. PCA was measured by intravenously injection with Ag (DNP-HSA in 1% Evans blue dye) to sensitized mice after oral administration of 25 and 50 mg/kg spinacetin or Dexa ( Figure 6A). Dexa was used as positive drug. As shown in Figure 6, IgE/Ag significantly induced PCA reaction, and treatment with spinacetin markedly decreased PCA reaction which is reflected by amount of diffused dye (Figures 6B,C) and ear thickness (Figures 6D,E), compared to the Ag challenged group. These attenuated allergic reactions might be due to the inhibitory effect of spinacetin on activation of mast cells. To confirm whether the decreased vascular permeability was related to amount of mast cells, the number of mast cells was counted at the ear reaction site. There was no variation between Ag treated group and spinacetin treated group (Figure 6F).

DISCUSSION
Flavonoids are abundant in plants, with a variety of biological functions such as anticancer, antiviral, antibacterial, antioxidative, and anti-inflammatory activities (Panche et al., 2016;Zakaryan et al., 2017). Particularly, anti-inflammatory activities of flavonoids were frequently reported. Luteolin suppresses inflammation-associated gene expression by blocking NF-κB and activating protein-1 (AP-1) activation (Chen et al., 2007). Quercetin reduces lipopolysaccharide (LPS)induced inflammation via inhibition of PI3K (Endale et al., 2013). Wogonin attenuates inflammation by activating PPAR-γ (Li et al., 2017). However, it has been rarely reported about the bioactivities of spinacetin. Recently, we isolated spinacetin from Inula japonica Thunb. In the present study, we report its anti-inflammatory effect on IgE/Ag-induced mast cells and PCA mouse model as well as the related molecular mechanism.
Aggregation of the high-affinity IgE receptor FcεRI results in exocytosis of granules and production of LTs and cytokines. After binding of antigen to IgE/FcεRI complexes, Lyn transphosphorylates the ITAMs of βand γchains of FcεRI, which are associated with the Src family tyrosine kinases Lyn and Fyn and with the non-Src tyrosine kinase Syk (Geldman and Pallen, 2015). Fyn, Lyn and Syk contribute to the formation of multimolecular signaling complexes that are coordinated by several adaptor molecules like LAT, Gads, Grb2, Gab2, and signaling proteins like PLCγ (Rivera et al., 2008). FcεRI -mediated PLCγ activation results in generation of IP 3 , which binds to its receptor on the endoplasmic reticulum leading to liberation of Ca 2+ from intracellular store. We found that spinacetin suppressed mobilization of Ca 2+ and phosphorylation of PLCγ (Figures 1D, 5A). The activated Vav, Grb2, and SOS lead to phosphorylation of ERK, JNK, and p38, and activation of cPLA 2 to release arachidonic acid (Siraganian, 2003). In the present study, we have clearly shown that spinacetin suppressed LTC 4 generation through cPLA 2 phosphorylation and translocation, associated with MAPKs activation (Figures 2B-D).
Crosslinking of FcεRI-bound IgE-molecules with multivalent antigen or allergen induces NF-κB activation (Klemm and Ruland, 2006). NF-κB is a nuclear transcription factor involved in various biological processes such as inflammation and tumorigenesis (Ghosh and Dass, 2016). Therefore, the involvement of spinacetin in IgE/Ag-induced activation of NF-κB was investigated. Spinacetin potently inhibited phosphorylation of Akt, phosphorylation and degradation of IκBα, and finally decreased p65 translocation (Figures 3C-H). These findings suggest that spinacetin effectively inhibits IgE/Ag-induced NF-κB activation, in which suppression of Akt phosphorylation might be involved.
In order to know whether spinacetin targeted any protein upstream of Akt, we investigated the effect of spinacetin on phosphorylation of PLCγ, LAT, Syk, Lyn, and Fyn. As a result, spinacetin inhibited phosphorylation of PLCγ, LAT, and Syk (Figures 5A-D). However, it did not affect Lyn and Fyn (Figures 5E-G). These results indicate that spinacetin inhibits Syk-dependent pathway in IgE/Ag-induced mast cells.
Anaphylaxis is the most serious allergic reaction, and typically occurs from the activation of mast cells and basophils through crosslinking of IgE and aggregation of FcεRI (Peavy and Metcalfe, 2008;Guilarte et al., 2017). In order to investigate whether spinacetin attenuates allergic response in vivo, we used a mast cell-dependent PCA model. The oral administration of spinacetin 1 h prior to challenge with Ag effectively impaired extravasation of Evans blue dye in the ear, and ear thickness (Figure 6), suggesting the in vivo anti-anaphylaxis efficacy of spinacetin.
In the present study, we demonstrated that spinacetin inhibited Syk, LAT, PLCγ activation, subsequently downregulated Akt, IκBα, NF-κB, MAPKs, cPLA 2 , and Ca 2+ mobilization, finally suppressed histamine release, LTC 4 synthesis, and IL-6 production in IgE/Ag-stimulated BMMCs (Figure 7). Spinacetin also inhibited the corresponding signals in IgE/Ag-induced RBL-2H3 cells, but did not affect those in PMA and A23187-induced HMC-1 cells. Furthermore, spinacetin attenuated IgE/Ag-mediated PCA reaction in mouse model. This study suggests that spinacetin could be used as a lead compound for development of novel anti-inflammatory drugs.

AUTHOR CONTRIBUTIONS
NJ, SP, CS, and YC performed the experiments. ZZ, RW, and YQ provided technical assistances. NJ and MJ wrote the manuscript. MJ and DK designed the experiments and acquired funding for the study. DK edited the manuscript.