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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Pharmacol. | doi: 10.3389/fphar.2019.00811

Cyclocarya paliurus triterpenoids improve diabetes-induced hepatic inflammation via the Rho-kinase dependent pathway

Cuihua Jiang1, Yiting Wang1, Qiaomei Jin2, 3, Dongjian Zhang3, Meng Gao3, Nan Yao3, Zhiqi Yin1,  Jian Zhang4* and Shiping Ma1
  • 1China Pharmaceutical University, China
  • 2Nanjing University of Chinese Medicine, China
  • 3Affliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, China
  • 4Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing Traditional Chinese Medicine Hospital, China

This study aimed to assess the effects of triterpene extract of Cyclocarya paliurus (Batal.) Iljinskaja (CPT) on diabetes-induced hepatic inflammation, and to unveil the underlying mechanisms. Diabetes in db/db mice was alleviated after CPT administration, as assessed by the oral glucose tolerance test. In addition, treatment with CPT dramatically reduced serum insulin, aspartate amino-transaminase, alanine aminotransferase, triglyceride and total cholesterol amounts. Besides, serum levels of interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α were also reduced after CPT administration. Western blot analysis revealed that CPT treatment significantly reversed the protein expression levels of Rho, ROCK1, ROCK2, p-P65, p-IκBα, p-IKKα, p-IKKβ in liver samples obtained from db/db mice. Upon palmitic acid stimulation, the protective effects of CPT on the liver were further assessed in HepG2 and LO2 cells, and no appreciable cytotoxic effects were found. Therefore, these findings indicates CPT alleviates liver inflammation via Rho-kinase signaling.

Keywords: Cyclocarya paliurus, triterpene, Diabetes Mellitus, Hepatic inflammation, ROCK, NF-κB

Received: 07 Nov 2018; Accepted: 24 Jun 2019.

Edited by:

Haitao Lu, Shanghai Jiao Tong University, China

Reviewed by:

Geng Wenye, Fudan University, China
Chen Ling, University of Florida, United States  

Copyright: © 2019 Jiang, Wang, Jin, Zhang, Gao, Yao, Yin, Zhang and Ma. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Jian Zhang, Nanjing Traditional Chinese Medicine Hospital, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China, zjwonderful@hotmail.com