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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Pharmacol. | doi: 10.3389/fphar.2019.00940

MicroRNA-532-3p suppresses malignant behaviors of tongue squamous cell carcinoma via regulating CCR7

Cuijuan Feng1, Hyon Il So2, Shoucheng Yin3, Xingzhou Su3, Qiang Xu3, Simin Wang3, Weiyi Duan3, Enjiao Zhang3, Changfu Sun3 and  Zhongfei Xu3*
  • 1Department of Orthodontics, School of Stomatology, China Medical University, China
  • 2Pyongyang Medical University, North Korea
  • 3Department of Oral and Maxillofacial Surgery, School of Stomatology, China Medical University, China

To provide better therapeutic avenues for treating tongue squamous cell carcinoma (TSCC), a series of experiments about the effects of microRNA (miR)-532-3p on TSCC malignant behaviors were carried out. The result showed that miR-532-3p was down-regulated and C-C chemokine receptor 7 (CCR7) was up-regulated in the tumor tissues compared with those in the paired paratumor tissues. Further, expression of miR-532-3p was detected in four TSCC cell lines, TSCCA, TCA8113, CAL-27, and SCC-25. The miR-532-3p mimics and inhibitor were transfected into the CAL-27 and TCA8113 cell lines which were relatively lowest and highest miR-532-3p expression, respectively. It was found that the overexpression of miR-532-3p suppressed TSCC cell proliferation, migration, invasion, and promoted apoptosis in vitro, whilst knockdown of miR-532-3p was reversed these behaviors. The bioinformatics predicted that CCR7 was a downstream gene of miR-532-3p, which was confirmed via luciferase assay. Following, the decline of CCR7 in the miR-532-3p mimics group and the rise of CCR7 in the miR-532-3p inhibitor group were also verified. In addition, enhanced cell proliferation, migration and invasion induced by CCR7 were partly restrained by miR-532-3p in TSCC cell. Meanwhile, miR-532-3p attenuated tumourigenesis in vivo due to the reduction of tumor volume and Ki-67 positive rate as well as the increase of apoptotic cells. Taken together, these findings reveal a pivotal role for miR-532-3p/CCR7 axis in regulating TSCC, and this novel axis could be suitable for therapeutic intervention in TSCC disease.

Keywords: MicroRNA-532-3p, CCR7, Tongue squamous cell carcinoma, proliferation, Migration, invasion, Apoptosis

Received: 09 Apr 2019; Accepted: 24 Jul 2019.

Edited by:

Chiranjib Chakraborty, Galgotias University, India

Reviewed by:

Bashir M. Rezk, Southern University at New Orleans, United States
Jeff Yi-Fu Chen, Kaohsiung Medical University, Taiwan  

Copyright: © 2019 Feng, So, Yin, Su, Xu, Wang, Duan, Zhang, Sun and Xu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Zhongfei Xu, Department of Oral and Maxillofacial Surgery, School of Stomatology, China Medical University, Shenyang, Liaoning Province, China, xzf7090@outlook.com