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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Pharmacol. | doi: 10.3389/fphar.2019.00989

Effects of Tianshu Capsule on Spontaneously Hypertensive Rats as Revealed by 1H-NMR-Based Metabolic Profiling

 Jian Gao1*, Tieshan Wang2, Chao Wang3*, Shuai Wang4, Wei Wang4, Di Ma4, Yongbiao Li5, Haibin Zhao4, 6 and  Jianxin Chen2
  • 1The Third Affiliated Hospital, Beijing University of Chinese Medicine, China
  • 2Beijing University of Chinese Medicine, China
  • 3Dongzhimen Hospital, Beijing University of Chinese Medicine, China
  • 4Beijing University of Chinese Medicine Third Affiliated Hospital, China
  • 5School of Life Science and Engineering, Southwest Jiaotong University, China
  • 6Dongfang Hospital, China

Hypertension is one of the most common cardiovascular diseases, resulting in serious complications, such as cardiovascular damage and chronic kidney disease. Tianshu capsule (TSC), composed of Chuanxiong (Ligusticum chuanxiong Hort) and Tianma (Gastrodiaelata Blume), has been widely used to treat the blood stasis type of headache and migraine in clinic. Results of previous study showed its antihypertensive effects, but the underlying mechanisms were still unclear. The purpose of this study was to evaluate the antihypertensive effect of TSC on spontaneously hypertensive rats by 1H NMR-based metabonomics and enzyme-linked immunosorbent assay (ELIAS), explore potential biomarkers and targets, and probe into the potential mechanism of TSC on antihypertensive treatment. The results showed that TSC could decrease the product of oxidative stress (MDA) and enhance the activities of SOD and GSH-Px, down-regulate the expression of enzymes (LDHA, PKM2 and HK2) related to glycolysis, and perturb a series of amino acids (isoleucine, alanine, asparagine, citrate, etc.) levels and pathways. Multivariate statistical analyses showed remarkable changes in some endogenous metabolites after administrating TSC relating to oxidative stress, amino acid metabolism and energy metabolism disturbances. Some enzymes (alanine-glyoxylate aminotransferase-2, tyrosine hydroxylase, dopa decarboxylase, etc.) related to metabolic biomarkers were predicted as the potential targets of TSC treatment on SHRs. The discoveries are helpful to understand the antihypertensive mechanism of TSC and provide a theoretical evidence for its future research, development and clinical use.

Keywords: Tianshu Capsule, Hypertensive, 1H-NMR, Metabolism, SHR

Received: 02 Jan 2019; Accepted: 31 Jul 2019.

Copyright: © 2019 Gao, Wang, Wang, Wang, Wang, Ma, Li, Zhao and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Dr. Jian Gao, Beijing University of Chinese Medicine, The Third Affiliated Hospital, Beijing, China,
Dr. Chao Wang, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, 100001, Beijing Municipality, China,