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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Pharmacol. | doi: 10.3389/fphar.2019.01383

Genome analysis reveals a synergistic mechanism of Ursodeoxycholic Acid and Jasminoidin in Mice Brain Repair after Ischemia/Reperfusion:crosstalk among muti-pathways

Yingying Zhang1, Haixia Li2, Huan Guo2, Bing Li3, Zide Zhao4,  Pengqian Wang3, Hongli Wu3,  Jun Liu3, Yinying Chen2, Xiao-xu Zhang4, Ping Wu2,  Zhong Wang5* and Jie Wang2
  • 1Dongzhimen Hospital, Beijing University of Chinese Medicine, China
  • 2Guang’anmen Hospital, China Academy of Chinese Medical Sciences, China
  • 3Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, China
  • 4China Academy of Traditional Chinese Medicine Hospital of Ophthalmology, China
  • 5China Academy of Chinese Medical Sciences, China

Studies have shown that combination drug therapy which corresponding treatment involves multiple genes and targets is more effective against cerebral ischemia.To identify the synergistic mechanism of ursodeoxycholic acid and jasminoidin based on differential pathway network , which protect against brain ischemia/reperfusion.A total of 115 mice with focal cerebral ischemia/reperfusion injury were divided into 5 groups: Sham, Vehicle, ursodeoxycholic acid (UA), jasminoidin (JA) and JA and UA combination group (JU). The differentially expressed genes identified by microarray arrays which consisted of 11644 cDNAs were loaded to the GeneGo MetacoreTM software to analyze the enriched pathways and processes among different groups.Among the top ten pathways and processes network,5, 6, and 3 overlapping pathways as well as 5, 3, and 4 overlapping processes network were observed between UA and JA, UA and JU, and JA and JU, respectively. Of which, 3 pathways and 3 process networks were shared across the three groups. Interestingly, 4 representative pathways and 6 process networks were only occured in the JU group. GO process analysis showed 2 processes were shared by all three treatment groups in the top ten process.The UA and JA combination resulted in synergistic effects through affecting muti-signal transduction pathways ,different locations in the same pathway and the new signaling pathway emergenced in drug combination group, those together may enhance the treatment of cerebral ischemia-reperfusion injury through promoting neural cell apoptosis,decreases calcium levels,inhibiting inflammation and protecting neurons.

Keywords: cerebral ischemia, Synergistic mechanism, GeneGo MetacoreTM software, signaling pathway, Network analysis

Received: 17 Jan 2019; Accepted: 30 Oct 2019.

Copyright: © 2019 Zhang, Li, Guo, Li, Zhao, Wang, Wu, Liu, Chen, Zhang, Wu, Wang and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Zhong Wang, China Academy of Chinese Medical Sciences, Beijing, China, zhonwangzy@sina.com