Tolerability, Safety, Pharmacokinetics and Drug Interaction of Cefotaxime Sodium–Tazobactam Sodium Injection (6:1) Following Single and Multiple Intravenous Doses in Chinese Healthy Subjects

Purpose To evaluate the tolerability, safety, pharmacokinetics and drug interaction of cefotaxime sodium–tazobactam sodium injection (6:1) in Chinese healthy subjects. The results of the safety and pharmacokinetic studies supported further clinical trials. Method A randomized, single-blind, ascending dose, placebo-controlled, single-center study was conducted. Sixty healthy subjects (38 males, 22 females) participated in this study. For the single-dose part, 0.47, 1.17, 2.34, 3.51, and 4.68 g of cefotaxime sodium–tazobactam sodium injection (6:1) was administered. For the multiple-dose part, the subjects were administered 2.34 and 3.51 g cefotaxime sodium–tazobactam sodium injection (6:1) three times a day for 7 consecutive days. For the drug interaction part, the subjects received 2.0 g cefotaxime sodium and 0.34 g tazobactam sodium alone and in combination. Results Most adverse events and adverse drug reactions were mild. Moderate rash was considered a serious adverse event because of prolongation of hospitalization. The main pharmacokinetic parameters of cefotaxime and tazobactam had no significance difference between the 1.17, 2.34, and 3.51 g dose cohorts and between genders. There was no difference in trough concentrations on days 6, 7, and 8. The RC max and RAUC were (0.921 ± 0.070) and (0.877 ± 0.057) for cefotaxime, and (0.913 ± 0.046) and (0.853 ± 0.060) for tazobactam, respectively. Following the administration of cefotaxime and tazobactam alone and in combination, the 90% conﬁdence intervals of the geometric mean ratios for C max and AUC were within the predetermined range of 80–125%. In the single-dose part, the renal cumulative excretion ratios were (51.7 ± 6.2)% for cefotaxime, and (84.3 ± 8.1)% for tazobactam. There was no significant difference in the maximum excretion rates and cumulative excretion ratios for cefotaxime and tazobactam, alone or in combination. Conclusions Cefotaxime sodium–tazobactam sodium injection (6:1) was well-tolerated at doses of 0.47 to 4.68 g. The pharmacokinetics of cefotaxime and tazobactam were reported as linear over a dose range of 1.17–3.51 g. Cefotaxime was partially excreted via urine, whereas tazobactam was mainly excreted via urine. There was no significant accumulation after administration over 7 consecutive days. The pharmacokinetics and excretion of cefotaxime and tazobactam were not affected by the co-administration of cefotaxime–tazobactam.


INTRODUCTION
As a safe and low-toxicity antimicrobial, cefotaxime (CTX), which belongs to the third-generation cephalosporins is a prescribed antibiotic agent (Agüero et al., 1999;Barbarina et al., 2001;Scanes et al., 2001). It is a broad-spectrum antibiotic which remarkable activity against both Grampositive and -negative bacteria (Koedijk et al., 2016;Beŕanger et al., 2017). CTX alone has been widely applied in clinical practice in the Chinese population (Agüero et al., 1999;Barbarina et al., 2001;Scanes et al., 2001). However, its efficacy has been seriously threatened by drug-resistant bacteria following wide clinical application (Consortti and Salgado, 2017;Lubna and Khan, 2017). However, the combination of a b-lactam antibiotic and a b-lactamase inhibitor has emerged to overcome this problem (Nguyen et al., 2014;Gao et al., 2016). Tazobactam (TAZ) is considered to have a stronger inhibitory effect on b-lactamases than firstand second-generation inhibitors (Papp-Wallace and Bonomo, 2016). The partner blactams, which can still play an antimicrobial role against a significant portion of extended-spectrum b-lactamases (ESBLs) (Gutieŕrez-Gutieŕrez et al., 2016), have been introduced into the clinical setting, such as piperacillin (PIP) and TAZ (Li et al., 2013;Chen et al., 2016;Murao et al., 2017), as well as ceftolozane (CLZ) and TAZ (Papp-Wallace and Bonomo, 2016;Kratzer et al., 2019). Cefotaxime sodium-tazobactam sodium (CTX-TAZ) (6:1) is a novel combination of b-lactam-b-lactamase inhibitor. b-lactamases can hydrolyze CTX, and TAZ can inactivate b-lactamase (Nguyen et al., 2014;Gao et al., 2016). CTX-TAZ (6:1) exerts synergistic antibacterial effect on drugresistant bacteria. Compared with CTX-TAZ (1:1), (2:1), (3:1), and (4:1), this ratio reduces the amount of TAZ without affecting its efficacy, thereby reducing toxicity and cost. As CTX is used to treat urinary tract infections (Luthy et al., 1981), it is necessary to determine the pharmacokinetic (PK) profile not only in plasma but also in urine. The objective of this study was to explore the tolerability, safety, and PK profiles after single-dose and multiple-dose administrations of CTX-TAZ (6:1) in Chinese healthy subjects. This study also evaluated the urinary excretion and drug interaction of CTX-TAZ (6:1). Our PK analysis could pave the way for clinical approval and determining the appropriate dosing regimen of CTX-TAZ (6:1) injection.

Study Subjects
The study was conducted according to the principles of the current Declaration of Helsinki and Good Clinical Practice. It was approved by the institutional review board of the Ethics Committee of the First Affiliated Hospital of Nanjing Medical University prior to the study on January 13, 2017 (Approval Number: 2017-MD-014.A2). The drug clinical trial approval number was 2005L02508, as given by the China National Medical Products Administration.
Healthy subjects aged 18 to 45 years with body mass index (BMI) of 18.0-26.0 kg/m 2 were included. They were deemed healthy based on a screening procedure that included clinical laboratory tests, 12-lead electrocardiogram (ECG), physical examinations and medical history. Sixty subjects without any significant cardiac, renal, gastrointestinal, hepatic, or metabolic conditions were eligible to participate in this study. All individual participants provided written informed consent before participating in any study-related procedures.

Study Design
This was a randomized, single-blind, ascending dose, placebocontrolled, single-center study, which was divided into three parts, a single-dose part, a multiple-dose part and a drug interaction part. Cefotaxime sodium-tazobactam sodium injection (6:1) (package: 1.17 g, bath no: 20160901), cefotaxime sodium injection (package: 1.0 g, bath no: 20160901) and tazobactam sodium injection (package: 0.17 g, bath no: 20160901) were provided by Nanjing Yoko Pharmaceutical Co., Ltd (Nanjing, China). Those were administered as a 1-h intravenous injection twice daily after being dissolved in 100 ml of 0.9% sodium chloride. The 0.9% sodium chloride injection was also used as a placebo.
The flow diagram (Figure 1) was to illustrate the progress of subjects through the trial.
For the single-dose part, 48 subjects were randomly assigned to five dose cohorts and received intravenous infusion of 0.47 g (cefotaxime sodium 0.4 g, tazobactam sodium 0.07 g), 1.17 g (cefotaxime sodium 1.0 g, tazobactam sodium 0.17 g), 2.34 g (cefotaxime sodium 2.0 g, tazobactam sodium 0.34 g), 3.51 g (cefotaxime sodium 3.0 g, tazobactam sodium 0.51 g), or 4.68 g (cefotaxime sodium 4.0 g, tazobactam sodium 0.68 g) of CTX-TAZ (6:1) ( Table 1). The next higher-dose cohort would be generated only after the safety and well-tolerability were confirmed in the previous dose cohort. Subjects received five different doses of CTX-TAZ (6:1) injection at 08:00 on the first day of study. Vital sign measurements, ECG, and safety assessments were performed at pre-dosing (0 h) and 1, 2, 4, and 8 h post-dosing. The urine-collected dose cohorts were banned from drinking water for 2 h after administration on the first day, and they should drink 200 ml of water at 2, 6, 8, 12, and 14 h after administration. The subjects of the 2.34 and 3.51 g dose cohorts continued to enroll in the multiple-dose part after completion of the single-dose part, administered CTX-TAZ (6:1) injection or a matching placebo three times a day for seven consecutive days ( Table 2). CTX-TAZ (6:1) injection was administered at 08:00, 16:00, 00:00 from day 2 (day 1 of the multiple-dose part) to day 8 (day 7 of the multiple-dose part), and at 08:00 on day 9.
For the drug interaction part, three cohorts each received doses of 2.0 g of CTX, 0.34 g of TAZ, and 2.34 g of CTX-TAZ (6:1) in a three-period, three-sequence, and crossover design ( Table 3). The procedure of the three-period study was the same as the single-dose part.  . For the drug interaction part, venous blood and urine samples were collected at the same time intervals as in the single-dose part. Following centrifugation at 3,500 rpm at 4°C for 8 min, plasma was separated and frozen at (−75 ± 10)°C until analysis. Urine samples were kept in a refrigerator at 4°C after collection. After each collection interval, the total volume was measured, and three aliquots of 1 ml were taken and stored in a refrigerator at (−75 ± 10)°C until analysis.

Analytical Assays
Plasma and urine samples were analyzed by two validated HPLC-MS/MS methods using Shimadzu LC-30AD (Kyoto, Japan) and Applied Biosystems/Sciex Triple Quad ™ 5500 (California, USA). The compounds were separated on an Ultimate XB-C18 column (50 × 2.1 mm, 3 mm; Welch) protected by a Security Guard Cartridges C18 (4 × 2.0 mm; Phenomenex) at 35°C. The solvents used for mobile phase consisted of methanol and solution A (0.05% acetic acid and 5 mM ammonium acetate). Mass spectrometry was performed using the negative electrospray ionization mode. Analytes were extracted from aliquots of 50.0 ml plasma or urine samples by protein precipitation with acetonitrile. The standard curves were linear over concentration ranges of 0.150-150 mg/ml for CTX and 0.0200-10.0 mg/mL for TAZ in plasma, as well as 4.00-5,000 mg/ml for CTX and 0.300-500 mg/ ml for TAZ in urine. The intra-day and interbatch precision and accuracy values well met the acceptance criteria according to the Guidance for Industry, Analytical Procedures and Methods Validation for Drugs and Biologics (2015) (U.S. Food and Drug Administration, 2015).

Safety and Tolerability
For safety monitoring throughout the study, all subjects remained in the study unit under continuous observation. Details of adverse events (AEs) and adverse drug reactions (ADRs) were obtained and recorded by the study physicians. Clinical evaluation was required for subjects who have completed all trials, as well as subjects who withdrew from the trial. Routine safety and tolerability were evaluated through the basis of vital signs, physical examination, AEs, laboratory examination (routine blood, urine, and fecal test; occult blood test; and blood biochemical test), ECG, and the situation of withdrawal; this evaluation was performed at scheduled intervals during the studies. AEs that occurred during the study were classified as mild (awareness of a sign or symptom, but comfortably tolerated), moderate (discomfort that may interfere with daily activities), or serious (death, life-threatening, requiring hospitalization or incapacitating). AEs and ADRs were recorded and reported according to the Good Clinical Practice.

Statistical Analysis
The sample size was set according to the guidance for clinical pharmacokinetic studies of chemical drugs, rather than the estimation of sample size. Descriptive statistics were used for safety and tolerability assessments. All AEs were analyzed together with the intensity and relationship to the medication and outcomes. The number and frequency of AEs and ADRs were calculated.
The PK parameters were assessed through noncompartmental analysis using Phoenix ™ WinNonlin version 6.4. Statistical analysis was performed by Phoenix ™ WinNonlin version 6.4 and SPSS version 19.0. In all cases, if the calculated P value was higher than 0.05, the difference would be considered statistically insignificant. For the single-dose PK study, one-way analysis of variance (ANOVA) and nonparametric test were used to compare PK parameters between the 1.17, 2.34, and 3.51 g dose cohorts.
The linear relationships of PK parameters between the three dose cohorts were tested. An independent-sample t-test and a nonparametric test were applied to analyze the differences in PK parameters between males and females. Urinary drug excretion was analyzed by the key indicators, cumulative excretion ratio and CTX, cefotaxime sodium injection; TAZ, tazobactam sodium injection; CTX-TAZ, Cefotaxime sodium-tazobactam sodium injection (6:1). maximum excretion rate. For the multiple-dose PK study, a oneway ANOVA was used to compare the trough concentrations on days 6, 7, and 8. The 95% confidence intervals (CIs) of the accumulation ratios, R Cmax (C ss_max of the multiple-dose part/ C max of the single-dose part), and R AUC (AUC ss of the multipledose part/AUC 0-t of the single-dose part), were estimated by Phoenix ™ WinNonlin version 6.4. After administration of CTX and TAZ alone and in combination, the possibility of clinically meaningful drug interaction was evaluated to determine whether the 90% CIs of the geometric mean ratios for C max and AUC were within the predetermined range of 80-125%. Paired-sample t-test was applied to compare the maximum excretion rates and the cumulative excretion ratios after CTX and TAZ administration alone and in combination.

Study Population
Sixty healthy subjects completed the trial. One subject from the 2.34 g dose cohort withdrew on day 8. Other demographic data are provided in Table 4.

Safety and Tolerability
In the single-dose part, eight AEs were reported in the 1.17 g dose cohort and one AE was reported in the 4.68 g dose cohort, while another subject treated with placebo reported three AEs. The incidence rates were 50.0% (2/4), 16.7% (1/6), and 12.5% (1/8), respectively. There was no ADR during this part. Those AEs were of mild intensity.
In the multiple-dose part, 4/10 (40.0%) subjects reported 12 AEs in the 2.34 g dose cohort and 2/10 (20.0%) subjects experienced five AEs in the 3.51 g dose cohort. In addition, 2/4 (50.0%) subjects, treated with placebo, reported two AEs. Five ADRs were reported by 3/10 (30.0%) subjects in the 2.34 g dose cohort and four ADRs were reported by 2/10 (20.0%) subjects in the 3.51 g dose cohort. Moreover, one ADR was reported in 1/4 (25.0%) subjects after placebo administration. Almost all AEs and ADRs reported in this part were of mild grade, with only two AEs reported as moderate (fever, rash). A subject had prolonged hospital stay due to moderate rash and discontinued the study on day 8 after multiple dosing in the 2.34 g dose cohort. Therefore, this AE was considered a serious AE (SAE) and determined to be probably related to the study medication.

Single-Dose Pharmacokinetics and Urinary Excretion Study
After single-dose administration, CTX and TAZ reached their C max within 0.667-1.07 h. The PK parameters of the 1.17, 2.34, and 3.51 g dose cohorts are presented in Table 5. T max , t 1/2 , MRT 0-8 , CL, and Vd were not significantly different between the doses of 1.0, 2.0, 3.0 g of cefotaxime sodium and 0.17, 0.34, 0.51 g of tazobactam sodium (P >0.05). The comparison of PK parameters between male and female subjects is presented in Table 6. The results of independent-samples t-test and nonparametric test showed that those PK parameters, ln(C max / dose), ln(AUC 0-t /dose), t 1/2 , MRT 0-8 , CL, Vd, and T max , had no significance difference between genders (P >0.05). The mean plasma concentrations versus time profiles of the analytes from the three dose cohorts are shown in Figure 2. One-way ANOVA revealed that the ln(C max /dose) and ln(AUC 0-8 /dose) of CTX and the ln(AUC 0-8 /dose) of TAZ were not significantly different between the three dose cohorts (P >0.05). The difference in the ln(C max /dose) of TAZ (P = 0.0190) may be due to the interindividual variability and the limited sample size. Therefore, it was not enough to regard this pharmacokinetics as nonlinear.
The relationships of C max and AUC 0-8 with dose are shown in Figure 3. The slopes b (PK = a * Dose b ) for the log-transformed C max and AUC 0-8 to the log-transformed doses were 1.03 and 1.05 of CTX, as well as 1.00 and 1.03 of TAZ, respectively (criteria range: 0.800-1.25). Therefore, this PK could be regarded as doseproportional. The 90% CIs of the slope were contained within an interval of 0.797-1.20, which also indicated dose proportionality.
The excretion rates of CTX and TAZ in urine reached peak values at (0.459 ± 0.068) g/h of CTX and (0.128 ± 0.015) g/h of TAZ over 1 h in the 2.34 g dose cohort. The mean cumulative excretion ratios of CTX and TAZ are shown in Figure 4. The cumulative excretion ratios of CTX and TAZ were (51.7 ± 6.2) and (84.3 ± 8.1)%, respectively.

Multiple-Dose Pharmacokinetics
The trough concentrations of the 2.34 g dose cohort on days 6, 7, and 8 were analyzed by one-way ANOVA. The results (P >0.05) showed no difference in the trough concentrations between days 6, 7, and 8. The plasma concentrations of CTX and TAZ all appeared to be at a steady state on day 6.
The R Cmax of CTX and TAZ were (0.921 ± 0.070) and (0.913 ± 0.046), the R AUC of CTX and TAZ were (0.877 ± 0.057) and (0.853 ± 0.060), respectively. The 95% CIs of CTX were 86.7-97.2% (R Cmax ) and 82.9-92.2% (R AUC ), and the 95% CIs of TAZ were 87.7-94.8% (R Cmax ) and 80.6-89.6% (R AUC ). The mean plasma concentration-time profiles on day 9 following the multiple-dose administration were similar to those on day 1 after the single-dose administration ( Figure 5). Furthermore, the comparison of PK parameters between multiple-dose and singledose administration is shown in Table 5. The PK parameters of CTX and TAZ were also similar on day 9 compared with those

Drug Interaction of Pharmacokinetics and Urinary Excretion Study
After administration of CTX and TAZ alone and in combination, the PK parameters of CTX and TAZ were measured, and the results are shown in Table 7. The concentration-time profiles of CTX and TAZ are shown in Figure 6. After CTX was administered alone and in combination with TAZ, the 90% CIs of the geometric mean ratios for C max , AUC 0-8 , and AUC 0-∞ were 101.0-110.4%, 102.4-110.8% and 102.5-110.9%, respectively, within a predetermined range of 80-125%. After TAZ was administered alone and in combination with CTX, the 90% CIs of the geometric mean ratios for C max , AUC 0-8 and AUC 0-∞ were 98.9-110.3%, 104.1-113.8% and 104.0-113.7% within a predetermined range of 80-125%. These findings indicated that the PK parameters of both CTX and TAZ were unaffected by the co-administration. The mean cumulative excretion ratios and standard deviations of CTX and TAZ when administered alone and in combination are shown in Figure 7. After administration of CTX alone, the maximum excretion rate and the cumulative excretion ratio were (0.384 ± 0.0586) g/h and (49.3 ± 7.1)%, respectively. When TAZ was administered alone, the maximum excretion rate and the cumulative excretion ratio of TAZ were (0.106 ± 0.0257) g/h and (75.8 ± 15.7)%, respectively. Similar results were observed when these two drugs were co-administered. After administration of CTX in combination with TAZ, the maximum excretion rate and the cumulative excretion ratio were (0.353 ± 0.128) g/h and (45.3 ± 14.5)% for CTX, and (0.105 ± 0.0396) g/h and (76.5 ± 26.8)% for TAZ. The results of the paired-sample t-test showed that there was no significant difference in the maximum excretion rates and cumulative excretion ratios for CTX and TAZ, both alone and in combination.

DISCUSSION
This study evaluated the PK profiles, safety and tolerability of CTX-TAZ (6:1) injection and the drug interaction of CTX and TAZ in Chinese healthy subjects. Single doses of 0.47 to 4.68 g of CTX-TAZ (6:1) were generally safe and well-tolerated. Multiple doses of 2.34 to 3.51 g of CTX-TAZ (6:1) injection administered for seven consecutive days were also generally safe and well-tolerated. AEs were observed in all dose cohorts and placebo cohorts. Most AEs and ADRs were mild, only two AEs were reported as moderate. In this study, no dose-limiting toxicities occurred in single doses up to 4.68 g and multiple doses up to 3.51 g for seven consecutive days. Therefore, those AEs appeared to be doseindependent. However, an AE, moderate rash, occurred in the 2.34 g cohort in the multiple-dose part was reported as a SAE for prolonging hospital stay. The incidence of ADRs with CTX ranges from 1 to 10%, and rash is a common reaction (Babu and Sharmila, 2011). Babu (Babu and Sharmila, 2011) reported a case of a 7-day-old male neonate with CTX-induced near-fatal anaphylaxis. De Koning et al. (De Koning et al., 1983) reported that one patient developed erythematous macular rash 2 days after treatment with CTX.
The PK parameters C max and AUC of CTX and TAZ were identified as linear over a dose range of 1.17-3.51 g of CTX-TAZ (6:1). In addition, semi-logarithmic graphs showed that both CTX and TAZ were linearly eliminated. In this study, we found that doses of 1.0, 2.0, and 3.0 g of cefotaxime sodium and 0.17, 0.34, and 0.51 g  of tazobactam sodium had no effect on the PK parameters, T max , t 1/ 2 , MRT 0-8 , CL, and Vd. Those findings in the present study were similar with previously published results. Xu et al. (2005) reported that there was no significant difference in t 1/2 between doses of 0.9, 1.8, and 4.5 g of cefotaxime sodium and 0.3, 0.6, and 1.5 g of tazobactam sodium. Moreover, we found that genders did not lead to significant differences in the PK behavior. The plasma concentrations of CTX and TAZ all appeared to be at a steady state on day 6 with no meaningful accumulation of CTX and TAZ after multiple dosing. Those results supported further clinical trial and clinical application of CTX-TAZ (6:1) injection. After CTX and TAZ were administered alone and in combination, the 90% CIs of the geometric mean ratios for C max and AUC were within the predetermined range of 80-125%. This result indicated that the C max and AUC of both CTX and TAZ were unaffected by the co-administration. Furthermore, Table 7 showed that the PK parameters, t 1/2 , T max , K, CL, Vd and MRT 0-8 were not affected by the coadministration of CTX and TAZ. Several reports have described the PK profiles and safety of CTX and TAZ individually or in combination with other drugs (Luthy et al., 1981;Hary et al., 1989;Wise et al., 1991;Urien et al., 2004;Miller et al., 2012). Luthy et al. (1981) reported that the t 1/2 of CTX was (0.72 ± 0.30) h in six healthy males after administration of CTX. Ings et al. (1985) reported that the t 1/ 2 of CTX varied between 0.92 and 1.65 h in three healthy males after intravenous dosing of CTX. Hary et al. (1989) reported that the t 1/2 of CTX was (1.59 ± 0.57) h in eight healthy subjects after administration of CTX. However, the sample size of those studies was small. Therefore, the main reasons for this difference might be individual differences. Wise et al. (1991) reported that the t 1/2 of TAZ was (1.13 ± 0.21) h in six subjects after a single intravenous injection of 0.5 g TAZ. Miller et al. (2012) reported that the average t 1/2 of TAZ was 0.970 and 1.10 h after single and multiple intravenous injection of TAZ, respectively. Those results also suggested that t 1/2 was not affected by the co-administration of CTX-TAZ.
In the single-dose part, the renal cumulative excretion ratios were (51.7 ± 6.2)% for CTX, and (84.3 ± 8.1)% for TAZ. Those data agreed with a previous study evaluating urinary excretion following co-administration of CTX-TAZ (3:1) (Xu et al., 2005). After administration of CTX alone, the cumulative excretion ratio was (49.3 ± 7.1)%. When TAZ was administered alone, the cumulative excretion ratio of TAZ was (75.8 ± 15.7)%. After co-administration, the cumulative excretion ratios were (45.3 ± 14.5)% for CTX and (76.5 ± 26.8)% for TAZ. Ings et al. (1985) reported that the urinary excretion of CTX was approximately 57%, and Luthy et al. (1981) reported that the urinary excretion was (53 ± 6)% after the administration of CTX alone. The cumulative excretion ratio of CTX after co-administration was in contrast to the cumulative excretion ratio of CTX administered alone in a previous research, which also suggested TAZ had no effect on the excretion of CTX. However, Wise et al. (1991) reported that the mean cumulative excretion ratio of TAZ was (63.7 ± 7.9)% when it was administered alone. However, the previous study obtained data from only six healthy males. The renal cumulative excretion ratios were also different between the single-dose part and the drug interaction part in this study. Therefore, the main reasons for this difference might be individual differences. Furthermore, the results obtained from patients were different, which was probably related to variability of the liver and renal function between healthy subjects and patients (Hary et al., 1989;Urien et al., 2004). Therefore, follow-up studies should include PK evaluation to develop more appropriate dosing regimens for patients.

CONCLUSIONS
Overall, CTX-TAZ (6:1) injection was a novel b-lactam-blactamase inhibitor combination with linear pharmacokinetics over a range of 1.17-3.51 g (1.0-3.0 g for cefotaxime sodium, 0.17-0.51 g for tazobactam sodium). It was also well-tolerated at doses of 0.47 to 4.68 g in Chinese healthy subjects. CTX and TAZ were rapidly cleared with a short t 1/2 . The doses of 1.0, 2.0, and 3.0 g for cefotaxime sodium and 0.17, 0.34, and 0.51 g for tazobactam sodium, as well as genders, did not lead to significant differences in the PK parameters. CTX was partially excreted via urine and TAZ was mainly excreted via urine. There was no significant accumulation after administration over 7 consecutive days in healthy subjects. The peak concentration, in vivo exposure, and the renal excretion were not affected by the co-administration of CTX and TAZ.

DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.

ETHICS STATEMENT
The studies involving human participants were reviewed and approved by the institutional review board of Ethics Committee of the First Affiliated Hospital of Nanjing Medical University. The patients/participants provided their written informed consent to participate in this study.