Quantification of Chlorogenic Acid and Vanillin from Coffee Peel Extract and its Effect on α-Amylase Activity, Immunoregulation, Mitochondrial Oxidative Stress, and Tumor Suppressor Gene Expression Levels in H2O2-Induced Human Mesenchymal Stem Cells

Background: Polyphenols and flavonoid-rich foods help in arresting reactive oxygen species development and protecting DNA from oxidative damage. Coffee peel (CP) preparations are consumed as beverages, and their total polyphenol or flavonoid content and their effect on oxidative stress–induced human mesenchymal stem cells (hMSCs) are poorly understood. Method: We prepared hot water extracts of CP (CPE) and quantified the amount of total polyphenol and flavonoid using HPLC analysis. In addition, CPE have been studied for their α-amylase inhibitory effect and beneficial effects in oxidative stress–induced hMSCs. Results: The obtained results show that the availability of chlorogenic acid, vanillin, and salicylic acid levels in CPE is more favorable for enhancing cell growth, nuclear integrity, and mitochondrial efficiency which is confirmed by propidium iodide staining and JC-1 staining. CPE treatment to hMSCs for 48 h reduced oxidative stress by decreasing mRNA expression levels of LPO and NOX-4 and in increasing antioxidant CYP1A, GSH, GSK-3β, and GPX mRNA expressions. Decreased pro-inflammatory (TNF-α, NF-κβ, IL-1β, TLR-4) and increased tumor suppressor genes (except Bcl-2) such as Cdkn2A, p53 expressions have been observed. Conclusions: The availability of CGA in CPs effectively reduced mitochondrial oxidative stress, reduced pro-inflammatory cytokines, and increased tumor suppressor genes.


INTRODUCTION
Globally, coffee is the most frequently consumed drink, and the increasing interest in coffee consumption has led the manufacturers to produce more varieties of coffee and is still progressive (Song et al., 2019). In the year 2019, the global green coffee production reached 10.03 billion kilograms (FAOSTAT, 2019). The large-scale production of coffee powder leads to an enormous amount of coffee peel (CP) waste (Murthy and Naidu, 2012;Prihadi et al., 2020). CP contains high fiber, and it has been identified with antioxidant, antiallergic, antihypertensive, and antimicrobial potential (Prihadi et al., 2020). The phytochemicals present in the CP are caffeine, tannin, polyphenol, pectin, and monosaccharide and disaccharide compounds (Janissen and Huynh, 2018). In coffee, the major beneficial bioactive compounds are caffeine and various polyphenols such as chlorogenic acid (CGA), ferulic acid, sinapic acid, gallic acid, and quinic acid (Hečimović et al., 2011;Durak et al., 2014). The main component of the phenolic fraction of the green coffee bean is CGA (Farah and Donangelo, 2006). CGA possesses a variety of health benefits such as reducing the risk of diabetes, cancer, and liver disease and protecting against Parkinson's disease (Bhupathiraju et al., 2013;Cano-Marquina et al., 2013).
Antioxidant compounds are majorly obtained from coffee and they contribute to the total dietary antioxidant capacity (Fujioka and Shibamoto, 2006). CGA is the main antioxidant compound in coffee which provides an activity similar to ascorbic acid (Pari et al., 2010). In addition, CGA prevents oxidized LDL and H 2 O 2induced oxidative stress and effectively scavenges the free radicals (superoxide, hydroxyl, and peroxynitrite) and protects DNA from oxidative damage (Cha et al., 2014). Meantime, excessive intake of coffee leads to higher bioavailability of CGA, which inhibits endothelial cells and cancer cell growth and angiogenesis; inversely, inhibition of angiogenesis and neovascularization cause impaired cell growth, tissue ageing, and functional disability (Wang et al., 2021). The present study emphasizes on CP, which contains high fiber and limited CGA, to be used as an antioxidant, antiallergic, antihypertensive, and antimicrobial agent (Prihadi et al., 2020).
CPs containing biomolecules need to be considered as a major antioxidant agent to protect against reactive oxygen species (ROS)-induced oxidative stress in human mesenchymal stem cells (hMSCs) during tissue repair or tissue regeneration therapy. Human MSCs play a major role in regenerative and immunomodulatory properties due to their multipotent differentiation potential (Denu and Hematti, 2016). Human MSCs are more sensitive to oxidative stress, and excessive ROS or exogenous H 2 O 2 can impair self-proliferation and multilineage capacity (Choo et al., 2014). They are implanted to injured tissues and contribute to tissue repair with suppressing inflammatory rejection (Di Nicola et al., 2002). The loss of transplanted MSCs at the ischemic site is a major problem due to the loss of chemokine receptors, after the generation of ROS at the graft site (Honczarenko et al., 2006). ROS are initially generated from the mitochondrial complex (I & III) and NOX4 during hMSCs differentiation (Devine et al., 2001). Excessive ROS react and damage the biomolecules, especially altering the integrity of genomic DNA, which is critical for cellular proliferation and functions (Kobayashi et al., 2012).
The present study aimed to quantify the concentration of CGA, vanillin, and salicylic acid (SA) in CP extract (CPE) using HPLC. Furthermore, the potential of CPE on α-amylase inhibitory effect, bioefficacy in hMSCs via analyzing the inhibitory effect of cell and nuclear damage, mitochondrial membrane polarization, oxidative damage, and immunomodulation-related gene expression levels in H 2 O 2induced oxidative stressed hMSCs have been explored.

Raw Material
Coffea arabica was obtained from the Jazan region in Fifa Mountains (1800 m above sea level), Kingdom of Saudi Arabia. Fresh whole coffee fruits were selected, washed, dried in the sun, and saved in a dry place until their extraction. CP was removed (hulled) from the whole coffee fruit and ground by using a coffee grinder (SF Stardust, PCP-R400065, Japan). Finally, the ground CP was sieved with stainless steel (0.5 mm) wire mesh to ensure a consistent powder size and kept at room temperature (25°C) for further extractions.

Preparation of the Extracts
CP was weighed (0.5, 0.75, 1, and 1.25 g) and then mixed with 10 ml water (solvent). Then the samples were boiled and stirred using a heating magnetic stirrer at 100°C for 10 min. Furthermore, the samples were allowed to rest for 10 min at room temperature and then centrifuged at 3000 g for 10 min using a fixed rotor centrifuge (Thermo Fisher Scientific, MA, United States). Finally, the samples were filtered using a filter paper (Whatman 9.0 cm). The obtained CPE was labeled properly, such as 0.5 g as CPE-1, 0.75 g as CPE-2, 1 g as CPE-3, and 1.25 g as CPE-4, and were kept under refrigeration at 4°C until analysis.

Total Polyphenol Content
The total polyphenol content (TPC) of the CPE was determined according to the method described by Hayat et al. (2011). 25 μl of the CPEs (CPE-1, CPE-2, CPE-3, and CPE-4) was mixed with 1500 μl distilled water and 125 μl Folin-Ciocalteu reagent (0.2%) and allowed to stand for 1 min. After that, 375 μl of Na 2 CO 3 (20% w/v) and 475 μl distilled water were added. Finally, the mixture was allowed to rest at room temperature for 30 min. The absorbance was measured at 760 nm using a spectrophotometer (JascoV-630 Spectrophotometer, United States). A blank was prepared without the extract. The TPC was expressed as a gallic acid equivalent per gram dry weight of the sample (mg GAE/g DW).

Total Flavonoid Content
The total flavonoid content (TFC) was determined as described by Hayat (2020). 250 μl of CPEs (CPE-1, CPE-2, CPE-3, and CPE-4) was mixed with 1000 μl of distilled water. Then, 75 μl of each NaNO 2 (5%) and AlCl 3 (10%) was added and incubated at room temperature for 5 min. Then, 500 μl of NaOH (1 M) and 600 μl of distilled water were added. The absorbance was recorded with a spectrophotometer (JASCO V-630 Spectrophotometer, United States) at 510 nm. A blank was prepared without the extract, and each sample was repeatedly analyzed six times (n 6) to get mean ± SD. The TFC was expressed as a catechin equivalent per gram dry weight of the sample (mg CE/g DW).

Carbohydrate Hydrolysis Enzymatic α-Amylase Assay
The carbohydrate hydrolysis enzymatic α-amylase (α-A) assay was performed according to the procedure suggested previously by Subramanian et al. (2008) and Hasenah et al. (2006). The controlled sample contained 150 μl of α-A enzyme solution and 150 μl of distilled water. The sample mixture was prepared by adding 150 μl of α-A to 150 μl of extract (CPE-1, CPE-2, CPE-3, and CPE-4). Likewise, the positive control was prepared with 150 μl of 1 mM acarbose (dissolved in 1% DMSO (dimethylsulfoxide)) and 150 μl of α-A. The blank mixture was prepared by dissolving 150 μl of distilled water with 150 μl of 20 mM phosphate buffer (pH 6.9) without α-A and the extract. Each tube was gently mixed and incubated at 37°C for 10 min. Thereafter, 150 μl of 0.5% starch solution was added to initiate the reaction, and the tubes were incubated at 37°C for 30 min. Then, 300 μl of dinitrosalicylic acid was added to stop the reaction, and the tubes were placed in a water bath at 100°C for 10 min. Lastly, all tubes were cooled to room temperature and then diluted with 2 ml of distilled water. The absorbance was determined at 540 nm (JASCO V-630 Spectrophotometer, United States). The percentage of amylase inhibitory activity of each sample was calculated by using the following equations: % Inhibition Adjusted control − Adjusted sample Adjusted control X 100. (1)

In Vitro Cell Culture Method and Materials
Human mesenchymal stem cells (hMSCs) have been obtained from the American type culture collection (ATCC, Manassas, VA, United States). DMEM (Dulbecco's Modified Eagle medium), EDTA (ethylenediaminetetraacetic acid), and trypsin were purchased from Gibco (Paisley, United Kingdom). Cell culture materials such as FBS (fetal bovine serum) and penicillin-streptomycin were obtained from Hyclone Laboratories, United States. MTT [3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide], propidium iodide, JC-1 stain, and all other chemicals related to a molecular biology experiment have been purchased from Sigma-Aldrich (St. Louis, MO, United States). The cell to cDNA synthesis kits and SYBR green PCR master mix were purchased from QIAGEN (Hilden, Germany).

Human Mesenchymal Stem Cell Culture
Human mesenchymal stem cells (hMSCs) were cultured using DMEM added with 10% FBS and 100 U/ml penicillin-streptomycin at 37°C in a humidified 5% CO 2 incubator. According to the experimental design and need, hMSCs were seeded in 96-well (1 ×10 4 cells/well) or 24-well (2 ×10 4 cells/well) plates containing DMEM with 10% FBS at 37°C and 5% CO 2 in humidified air. After visual confirmation of 80% confluence under an inverted microscope, the culture was used for the experiments.

Cytotoxicity Analysis
Human mesenchymal stem cells (hMSCs) were cultured in a 96well culture plate (1 × 10 4 cells/well) and allowed to adhere overnight to the growth medium. After discarding the medium, a culture medium containing an increasing concentration (1, 2, 4, 8, 16, 32, and 64 mg/ml) of CPEs, namely, CPE-1, CPE-2, CPE-3, and CPE-4, was added to each well, and the cells were incubated for 48 h; control cells were treated with vehicle alone. After completion of 48 h, the cells were added with 20 µl/well of MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] at a concentration of 5 mg/ml in DMSO and incubated at 37°C for an additional 4 h. At the end of incubation, the supernatant solution was removed gently without disturbing the formed purple formazan crystals. The crystal was dissolved in 100 µl of 100% DMSO using a multiwell plate shaker. The absorbance of the solution was measured at 570 nm using a microplate reader (Thermo Scientific). Cell proliferation (%) was calculated by the following equation: (absorbance of the sample/mean absorbance of the control) × 100.

Experimental Design
Bioefficacy and oxidative stress inhibition capacity of CPE on normal and H 2 O 2 -induced oxidative stressed hMSCs were examined. Two sets of hMSCs were cultured in 24-well plates and treated with 4 mg/ml of CPEs, namely, CPE-1, CPE-2, CPE-3, and CPE-4, for 48 h, respectively. After incubation, one group was kept for normal observation, and the other group of hMSCs were treated with 10 μM of H 2 O 2 for 30 min. CGA was used as a reference control in both the experimental sets. After incubation, all the grouped cells were used for propidium iodide staining, JC-1 staining, and gene expression analysis.

Propidium Iodide Staining Assay for Nuclear Damage
Cellular morphology for characteristic nuclear damage, pyknosis, or apoptotic morphological changes after hMSCs were treated with CPE-1, CPE-2, CPE-3, and CPE-4 (with or without H 2 O 2 ) was determined using propidium iodide (PI) staining analysis under inverted fluorescence microscopy as described by Leite et al. (1999).

Assay of Mitochondrial Membrane Potential (Δψ m ) by JC-1 Dye Staining
The mitochondrial membrane potential (Δψ m ) was determined using the JC-1 dye to assess mitochondrial efficiency in the vehicle control, and CPE-1-, CPE-2-, CPE-3-, and CPE-4-treated hMSCs (with and without H 2 O 2 ). Briefly, the JC-1 staining solution (mixed with equal volumes of the culture medium) was added to experimental hMSCs and incubated for 20 min in the dark at 37°C. After incubation, the unbound JC-1 dye was gently removed by washing with 200 μl of JC-1 staining wash buffer at 4°C, repeatedly for two times. Then, the accumulation of J-aggregates against JC-1 staining was observed under a florescence microscope.

Statistical Analysis
All the experiments were performed in triplicates, and the data were presented as mean values ± SD (standard deviation). SAS software (version 9.2, 2000-2008; SAS Institute Inc., Cary, NC, United States) was used to analyze the differences among the groups by one-way analysis of variance (ANOVA), and if significant differences were found, then the Duncan's multiple range test was conducted at a confidence interval of 95% (p < 0.05).

RESULTS AND DISCUSSION
Recently, there has been a surge of interest in phenolic compounds extracted from plant materials (Kähkönen et al., 1999;Tapiero et al., 2002;Murthy and Naidu, 2012). They are secondary metabolites that occur naturally in plants and are generally intricate in defense against oxidative stress or aggression by pathogens. Considerable populations in Europe and the Arab regions consume hot water-boiled dried CP powder as an energy drink or refreshing drink as a replacement for coffee (Liang and Kitts, 2016;Farah and de Paula, 2019). The polyphenol compound CGA is the major antioxidant present in the coffee seeds and peel (Ameca et al., 2018). Meanwhile, CGA is a more thermolabile compound, and depending upon the different hot preparation or extraction methods, a considerable amount of CGA is lost (Liang and Kitts, 2016). The processes to extract phenolic compounds vary from plant to plant. Thoo et al. (2010) demonstrated that the extraction efficacy is dependent on various factors such as the method of extraction, extraction time, solvent concentration, solvent type, temperature, and solid-tosolvent ratio. During extraction of phenolic compounds, the key effect of the solid-to-solvent ratio was applied to adjust the solubility and equilibrium constant, resulting in a maximum yield of bioactive compounds at the optimum solid-to-solvent ratio (Tan et al., 2011) and avoidance of saturation effect, as well as a reduction of the solvent waste disposal cost (Ho et al., 2008). The current research, on the other hand, demonstrated the effectiveness of aqueous extraction which is also known as a nonpolluting solvent for obtaining phenolic constituents. Many studies have been carried out in coffee, such as the optimum condition and extraction methods have been well explored, to attain maximum bioactive constituents with antioxidant potency from their raw materials (Boeira et al., 2018;Santos et al., 2014).

Total Polyphenol Content and Total Flavonoid Content in Coffee Peel
The TPC of the CPE has been shown in Figure 1A. A sample of CPE-1 (0.5 g CP) exhibited significantly (p ≤ 0.05) the highest amount of polyphenol. An inverse relationship has been observed between the TPC and the concentration of CP. That is, the sample containing the least concentration of CP exhibited the highest TPC (CPE-1 > CPE-2 > CPE-3 > CPE-4). The TFC of the CPE has been shown in Figure 1B. A similar pattern of TPC has been observed in TFC also. Likewise, an inverse relationship has been observed between TFC and the concentration of CP. That is, the sample containing the least concentration of CP showed the highest TFC (CPE-1 > CPE-2 > CPE-3 > CPE-4). A significant (p ≤ 0.05) difference in TFC has been observed between the samples except between CPE-3 and CPE-4. In this study, the TPC values ranged from 11.65 to 15.23 GAE mg/g CP and the TFC values ranged from 20.24 to 27.22 mg catechin equivalent. Our results are in line with those of Alkaltham et al. (2020). They reported the comparison of the TPC and TFC of coffee fruit beans, pulp, and parchment; the TPC was 0.392, 0.183, and 0.110 mg GAE/g DW, respectively, and the TFC was 30.30, 8.02, and 0.638 mg CE/g DW, respectively. In this context, Silva et al. (2021) (2021) confirmed that water: ethanol mixture was the extracting solution with a higher potential, and dehydration was a very significant factor to concentrate and provided higher levels of phenolic compounds. Andrade et al. (2012) reported that TPC ranged from 16.1 mg CAE/g to 423 mg CAE/g for coffee husk. The difference in results might be due to the differences in the variety and the extraction methods and extricating solvents used.

Quantification of Phenolic Compound in Coffee Peel Extracts Using HPLC
Individual polyphenols from coffee extracts (CPE-1, CPE-2, CPE-3, and CPE-4) were separated by HPLC and determined quantitatively. The major phenolic compounds which were found in CPE are presented in Table 1, and the results are expressed in mg/g. Chromatograms of the standard phenolic compounds and samples have been presented in Figures 2A,B, respectively. It has been found that the amount of CGA present in 100 g of CPE ranges from 589.9 to 871.0 mg. The highest amounts of CGA present in CP are as follows: CPE-3 > CPE-1 > CPE-2 > CPE-4 ( Table 1). The observed results confirmed that there was no significant correlation between the CGA quantity and the increasing amount of CP [CPE-1 (0.5 g), CPE-2 (0.75 g), CPE-3 (1 g), and CPE-4 (1.25 g)] taken for extraction. CPE-3 (1 g of CP) possessed a higher amount of phenolic compounds, such as CGA (871.0 mg/100 g), vanillin (409.6 mg/100 g), and salicylic acid (79.6 mg/100 g). As mentioned in Table 1, the average quantitative data showed that the CGA which is acknowledged for its antioxidant capacities ranged from 589.9 to 871.2 mg/100 g phenolic compound. The amount of vanillin ranged from 301.8 to 409.6 mg/100 g phenolic compound, and salicylic acid was the lowest (79.6-49.8 mg/100 g phenolic compound) among all. In this context, Jaiswal et al. (2012) have reported that the different roasting methods of coffee seeds in the laboratory consist of 40-209 mg/100 g in Coffea arabica and 40-509 mg/100 g in Coffea canephora seeds. In general, the level of CGA in coffee brews varies largely from 26 mg/100 ml to the extreme of 1141 mg/100 ml in different countries (Farah and de Paula, 2019). The amount of CGA differs due to various factors such as the species, the degree of maturation, the altitude, and the presence or absence of shade, as well as resistance to some diseases (Aerts and Baumann, 1994). According to our observation, the quantity of the CGA range in CP was found to be 589.9-871.0 mg/100 g in comparison with that in coffee seeds which varied from 200 to 301 mg/100 g. CGA is the main phenolic compound present in coffee beans that possesses antioxidant properties (Andrade et al., 2012;Clifford et al., 2017;Alkaltham et al., 2020). However, the CP contains a rich amount of polyphenols CGA along with total flavonoid, vanillin, and salicylic acid which favors effective inhibition of oxidative stress-associated cellular senescence and DNA damage (Xu et al., 2012;Cinkilic et al., 2013;Ameca et al., 2018).

Carbohydrate Hydrolysis Enzymatic α-Amylase Assay
Starch is one of the main sources of dietary energy which is mainly digested in the gastrointestinal tract by pancreatic α-amylase. Rate of starch digestion and absorption may help to control postprandial hyperglycemia, and so in diabetics, decelerating the digestion of starch may have a favorable effect on the glycemic index (Notkins, 2002). Hyperglycemia has been found to be linked with the threat of various diseases, such as obesity and cardiovascular-and kidney-related issues, which in turn increases the necessity for strict glycemic control (Blaak et al., 2012). In the intraluminal phase, α-A is the main enzyme accountable for starch digestion. So, an α-A inhibitor will slow down the carbohydrate digestion (Golay et al., 1991). Four extracts from the CP (CPE-1, CPE-2, CPE-3, and CPE-4) were tested for porcine pancreatic α-A enzyme inhibition. All extracts weakly inhibited porcine pancreatic α-A. The extract CPE-1 (0.5 g) provided the highest inhibitory effect on α-A (19.88%/mg extract). It was able to inhibit porcine pancreatic α-A by 20%. We observed in CPE-2, CPE-3, and CPE-4 a lesser than 20% inhibition of porcine pancreatic α-A with an insignificant (p ≥ 0.05) difference. IC 50 can be defined as the lowermost concentration of a compound that is required to inhibit 50% of the enzyme activity (Oboh et al., 2015). Acarbose was able to inhibit the enzyme α-A at 70% as shown in Figure 3. For acarbose, an IC 50 of 0.25 g/ml was determined ( Figure 3). The inhibition by acarbose was higher when compared to CPE. α-A is one of the most significant digestive enzymes in humans, and it works as a catalyst in the reaction which implicates the hydrolysis of alpha-1,4 glycosidic linkages of large molecules like starch into smaller fragments of sugars, i.e., monosaccharides. The sugar level in the blood rises due to excess conversion of starch to sugar in response to which the insulin instructs cells to metabolize the surplus sugar and store them in the form of sugar. This cycle keeps on going interminably until in certain conditions where the activity of the amylase enzyme increases or a deficiency of insulin or insulin resistance occurs which in turn leads to an increase in blood sugar (Agarwal and Gupta, 2016). There are two main biochemical mechanisms related to raised blood glucose levels, and they are enzymatic (the polyol pathway) and nonenzymatic glycosylation (Creutzfeldt, 1999). Acarbose is used for treatment of diabetes, and it inhibits the activities of α-A (Jyothi et al., 2014). In this study, the observed inhibition of α-A even though it was weak might be due to the presence of CGA, which is inconsistent with earlier studies (Ranilla et al., 2010;Narita and Inouye, 2011). The inhibition of α-A by flavonoids is due to the formation of hydrogen bonds by hydroxyl groups with specific amino acids at the enzymes' active sites (De Sales et al., 2012). The extract CPE-1 (0.5 g) and CPE-2 of CP provided the highest inhibitory effect on α-A (19.88 and 16.07%/mg extract), which has been identified with the highest TPC and TF content compared to other extracts.
Moreover, lower levels of TPC and TFC (µg/mg of extract) show lowest inhibition (statistically insignificant differences were observed between CP3 and CP4).

In Vitro Cell Culture Study Using hMSCs
Cells can normally protect themselves from ROS damage through their self-defense antioxidative mechanism. But, hMSCs have less antioxidant capacity and are more sensitive to oxidative stress, when compared to differentiated lineages such as adipocytes, chondrocytes, and osteoblasts (Yagi et al., 2013). In regenerative medicine, cellular stress produces excessive ROS or exogenous addition of H 2 O 2 might impair the capacity of differentiation to multiline ages or self-renewal and proliferation were impaired (Zou et al., 2004). Excessive free radical damage to hMSCs might end with cell senescence and arrested cell divisions (Bajek et al., 2012). Orciani et al. (2010) reported that ROS inhibits hMSCs to osteogenesis differentiation and undifferentiated hMSCs, having a higher lactate production rate and glycolytic enzyme levels. Lesser osteogenic differentiation results in bone weakness and arthritis. In contrast, ROS increases adipocyte differentiation with upregulated antioxidant gene expressions, similar to osteogenic differentiation mitochondrial biogenesis of glycolysis, and the lactate level increased in adipogenesis (Denu and Hematti, 2016).
The quantified rich TPC and TFC of CPE favors the antioxidant and oxidative stress quenching capacity. It was confirmed by the present study that increasing the concentration of CPE treated to hMSCs resulted in increased cell proliferation and viability in CPE-2 (94%) when compared to the other extracts [CPE-3 (79%) or CPE-4 (74%)] (Figure 4). In addition, CPE-2 showed significantly (p ≤ 0.05) increased cell viability (85%) against the increasing concentration of extract treatment. It may be due to the availability of rich amounts of phenolic and flavonoid components in CPE-2. The observed results were compared with the reference drug, CGA. In this context, Chen et al. (2008) have reported that the presence of antioxidant polyphenols, such as epigallocatechin and tocopherol, protects the hMSCs from oxidative stress and increased proliferation capacity. Phenolic compounds and phenols are widely distributed from natural agents, so they have been considered as powerful agents for their antioxidative activities in hMSCs.
In propidium iodide (PI) staining analysis, CPE-2-treated cells were compared with CGA-treated cells; only 3% of the cells showed a nuclear morphology change in CPE-2 treatment, but CGA (80 μM) treatment showed 11% of different nuclear morphology ( Figure 5A). H 2 O 2 -induced oxidative stressed hMSCs were treated with TPC-and TFC-rich CPE-2, with very less number (7%) of nuclear damaged cells, and H 2 O 2 + CGA-treated cells showed 16% of morphologically different cells that were observed in PI nuclear staining. But, H 2 O 2 alone treated cells showed 27% of pyknosis, and chromatin condensation was observed under an inverted microscope manually ( Figure 5B). The observed results are in line with the previous reports that higher ROS levels cause cellular damage and dysfunction. The accumulation of ROS can damage cellular DNA, glycoproteins, and glycolipids. Human patients with atherosclerosis and diabetes have been identified with elevated oxidative stress and reduced capacity to inhibit T-cell proliferation (Mancini et al., 2015). Previously, Cha et al. (2014) have reported that CGA effectively protects oxidative stress induced by DNA damage in human keratinocytes.
Alterations in mitochondrial electrochemical gradient and transmembrane potential majorly affect the cellular ATP production progress. The mitochondria use oxidizable substrates majorly to produce an electrochemical proton gradient across the mitochondrial membrane (De Mello et al., 2018). Mitochondrial oxidative stress alters the inner membrane polarity and leads to a loss of mitochondrial transmembrane potential (Δψ m ) further deregulating mitochondrial electronegative and transport capacity (Zorova et al., 2018). The internal electronegative organelle of the mitochondria promotes internal uptake of cations and outward transport of anions (ATP). During excessive ROS or free radicals, the mitochondrial permeability process decreases and a loss of the electrochemical gradient which is the major indicator for mitochondrial function and cell health occurs (Sivandzade et al., 2019). Regulation of energy homeostasis has been majorly controlled by the mitochondria, and mitochondrial imbalance has been associated with the development of obesity (Picard et al., 2011). Polyphenols from plant sources have been described as antioxidants; they have the potential to eliminate ROS and free radicals, which normalize the mitochondrial membrane potential (Robb et al., 2017). In the present study, a rich amount of polyphenols and flavonoids in CPE-2 significantly enhanced the mitochondrial health when compared to the other extracts ( Figure 6). It has been confirmed by the healthy electronegative mitochondria uptake of the cationic natural green florescent JC-1 dye, which was further converted internally into an irreversible red florescent J-aggregate. CPE-1 also showed moderate J-aggregate accumulation in oxidative stressed hMSCs; this effect was not observed in normal hMSCs. But, in CPE-3 and CPE-4, the conversion of J-aggregates from JC-1 was not observed both in normal as well as in oxidative stressed hMSCs, confirming the unhealthy mitochondria with a decreased mitochondrial membrane potential and membrane leakage. In addition, changes in mRNA expression levels in oxidative stress, and immunomodulatory and tumor suppressive-related genes in normal and H 2 O 2 -induced oxidative stressed hMSCs after polyphenol rich CP treatment were observed. The results confirmed that the increased antioxidant and mitochondrial membrane potential capacity of CPE-2 significantly (p ≤ FIGURE 5 | Analysis of vehicle control and CPEs-treated hMSCs nuclear morphology by PI Staining for nuclear damage (A) and manual counting for apoptotic or necrosis cells (B) after 48 h. All the images were captured with ×20 magnification. CPE-1 0.5 g, CPE-2 0.75 g, CPE-3 1 g, and CPE-4 1.25 g. Each of the values are mean ± SD (n 6). *p ≤ 0.05 and **p ≤ 0.001 were compared with vehicle control or H 2 O 2 alone treated hMSCs.
Frontiers in Pharmacology | www.frontiersin.org November 2021 | Volume 12 | Article 760242 0.001) decreased the oxidative stress markers of LPS and NOX-2 when compared to the untreated or H 2 O 2 alone treated hMSCs ( Figure 7A). The effect was observed in CGA-treated hMSCs with a significant level of p ≤ 0.05. The expression level of CYP1A was significantly (p ≤ 0.001) increased in CPE-2 treatment, and it was supported by the increased mitochondrial health (in JC-1 assay) after CPE treatment. The antioxidant potential of CPE-2 treatment was confirmed by the increased expression levels of GSH, GSK-3β, and GPx when compared to those of vehicle control or oxidative stress-induced hMSCs ( Figure 7B). In FIGURE 6 | Analysis of mitochondrial membrane potential using JC-1 staining for vehicle control and CPEs-treated hMSCs after 48 h. All the images were captured with ×20 magnification (CPE-1 0.5 g, CPE-2 0.75 g, CPE-3 1 g, and CPE-4 1.25 g). regenerative medicine, due to the multipotent differentiation potential of the hMSCs, these are implanted for tissue loss and contribute to tissue repair with suppressing inflammatory rejection (Di Nicola et al., 2002). After the generation of excessive ROS or exogenous H 2 O 2 at the ischemic site, the transplanted MSCs might impair self-proliferation and multilineage capacity (Choo et al., 2014). The present finding confirmed the increased expression of antioxidant genes that was associated with quenching of oxidative stress aid to overcome the impairment of multiline age capacity of hMSCs at the graft site. Pro-inflammatory cytokines and cellular metabolic inflammation-related gene expression levels also significantly (p ≤ 0.001) decreased in CPE-2-treated cells. TNF-α and NFκb expression levels were observed twofold higher in oxidative stressed hMSCs. IL-1β and COX-2 expressions were significantly increased to a fold in untreated and oxidative stressed cells when compared to the CPE-2-treated cells ( Figure 7C). In addition, tumor suppressor-related genes cdkn2a and p53 expressions were significantly decreased twofold when compared to oxidative stressed hMSCs. BCL-2 expression was decreased in CPE-2-treated and CGA-treated hMSCs both in normal and H 2 O 2induced oxidative stressed hMSCs ( Figure 7D). In response to diverse stresses such as DNA damage or hypoxia, the tumor suppressor p53 is accumulated and cellular proliferation arrested (Subash-Babu et al., 2017). Upon oxidative stress or hyperproliferation signaling conditions, mdm2 negatively regulates p53 which leads to transition from the resting phase (G1) to DNA synthesis phase (S), and subsequently, cancer cell progression continues. In unstressed cells, p53 is tightly regulated by murine double minute 2 (MDM2) by maintaining p53 at low levels. The cell-cycle gatekeeper gene p 14ARF neutralizing mdm2 function via cyclin-dependent protein kinase (Cdkn2A) leads to increased levels of active p53 (Chin et al., 1998).
External stimulus of oxidative stress or cellular stress causes DNA damage or cellular senescence. CP has the potential to overcome hypoxia and oxidative stress, therefore, it retains bioactive, secondary metabolites such as phenolic compounds, alkaloids, and flavonoid compounds. We identified CPE having a rich amount of polyphenol-CGA, vanillin, and salicylic acid. External stimulus of oxidative stress to hMSCs regulates stress-induced DNA damage, cell senescence, and impaired multilineage. But, CPE treatment to stressed hMSCs effectively downregulated the pro-oxidant LPO and NOX2 and enhanced the antioxidant mRNA. In addition, CP treated to stressed hMSCs potentially downregulated pro-inflammatory cytokines and enhanced tumor suppressor expressions, which is more beneficial for the cellular multilineage, such as osteogenic differentiation and cellular regeneration progress. In this context, several plant-derived compounds were found to fight against a wide range of cancer models, such as the colon, breast, liver, and prostate cancer suppression models (Wang, et al., 2012).

CONCLUSION
The present findings confirmed that the presence of total polyphenol-CGA, vanillin, and salicylic acid in CPE-2 (0.75g/ 10 ml) effectively inhibits pancreatic α-A inhibition and arrests H 2 O 2 -induced oxidative stress that may be due to the antioxidative nature of polyphenol and flavonoids. The antioxidant capacity of CP quenches the oxidative stress in hMSCs which decreases mitochondrial oxidative stress. Overall, the oxidative stress, and pro-inflammatory and tumor suppressor gene expression levels were normalized in CPEtreated oxidative stressed hMSCs. The present study confirmed that CP-derived polyphenol and flavonoid effectively quench the oxidative stress in hMSCs, which support to protect themselves from free radical or ROS damage through its self-defensive antioxidative mechanism during cellular multilineage and regenerative therapy.

DATA AVAILABILITY STATEMENT
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.