This article is a correction to:
Scorpion Venom Heat-Resistant Peptide Attenuates Microglia Activation and Neuroinflammation
Xue-Fei Wu1,2†
Chun Li3†Guang Yang4†Ying-Zi Wang2†Yan Peng1
Dan-Dan Zhu1
Ao-Ran Sui1
Qiong Wu1Qi-Fa Li1
Bin Wang1
Na Li2Yue Zhang1Bi-Ying Ge2Jie Zhao2*
Shao Li1,2*- 1Liaoning Provincial Key Laboratory of Cerebral Diseases, Department of Physiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China
- 2National-Local Joint Engineering Research Center for Drug-Research and Development (R&D) of Neurodegenerative Diseases, Dalian Medical University, Dalian, China
- 3Reproductive Medicine Centre, Affiliated Zhongshan Hospital of Dalian University, Dalian, China
- 4Department of Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
A Corrigendum on
Scorpion Venom Heat-Resistant Peptide Attenuates Microglia Activation and Neuroinflammation
by Wu, X.-F., Li, C., Yang, G., Wang, Y.-Z., Peng, Y., Zhu, D.-D., Sui, A.-R., Wu, Q., Li, Q.-F., Wang, B., Li, N., Zhang, Y., Ge, B.-Y., Zhao, J., and Li, S. (2021). Front. Pharmacol. 12:704715. doi: 10.3389/fphar.2021.704715
In the original article, there was a mistake in Figure 1 and its caption as published. The label for group 4 in Figure 1A is misspelled, it is supposed to be “LPS+SVHRP” instead of “LPS+SVHRSP”. Furthermore, in the caption for Figure 1, the description for Figure 1E was missing, which is the quantification result for the immunoblotting shown in (D). The correct Figure 1 and caption appear below.
FIGURE 1
FIGURE 1. SVHRP inhibits inflammagen-induced microglia activation and inflammatory response in hippocampus. Mice were injected with SVHRP (LPS + SVHRP or SVHRP group, 125 µg/5 ml/kg, i.p.) or NS (LPS or CTRL group, 1 ml/kg, i.p.) for 3 days before and 1 day after LPS treatment (5 mg/kg, i.p.) before the mouse brains were harvested for immuno-staining for Iba-1. (A) Representative images of Iba-1 staining (IF staining, upper three panels and IHC staining, lower two panels) in hippocampus were demonstrated. (B) The average percent area of Iba-1 positive staining of four groups was analyzed using the images from IHC staining. (C) mRNA expressions of TNF-α and iNOS from hippocampus were measured by real-time PCR and calculated using 2−ΔΔCT method with GAPDH as the internal reference gene. The expression of iNOS protein from hippocampus was assessed by western blot. (D) Representative blot for iNOS and (E) quantification of iNOS protein normalized to β-actin. The data were expressed as the means ± SEM (n ≥ 3 for each group). #p < 0.05 compared with CTRL group, *p < 0.05 compared with LPS group.
Additionally, there was a mistake in the caption for Figure 2 as published. In Figure 2, the value of the Bar in Figures 2A–C, a is supposed to be 50 instead of 20 μm. The correct Figure 2 appears below.
FIGURE 2
FIGURE 2. SVHRP attenuates LPS-induced upregulation of Iba-1 in microglia. IF staining for Iba-1 in primary neuron-glia (A), mixed glia (B), and enriched microglia (C) cultures were performed 24 h after LPS treatment. Cells were pretreated with vehicle or SVHRP (20 μg/ml) for 1 h before LPS challenge. (a) Representative images of Iba-1 positive cells (400×, Bar = 50 μm). (b) Cell number and average grey scale for Iba-1 staining were shown. The data were the means ± SEM (n ≥ 3 for each cell preparation). #p < 0.05 compared with CTRL group, *p < 0.05 compared with LPS group.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Publisher’s Note
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Keywords: SVHRP, anti-inflammation, microglia, NF-κB, MAPKs
Citation: Wu X-F, Li C, Yang G, Wang Y-Z, Peng Y, Zhu D-D, Sui A-R, Wu Q, Li Q-F, Wang B, Li N, Zhang Y, Ge B-Y, Zhao J and Li S (2021) Corrigendum: Scorpion Venom Heat-Resistant Peptide Attenuates Microglia Activation and Neuroinflammation. Front. Pharmacol. 12:791953. doi: 10.3389/fphar.2021.791953
Received: 09 October 2021; Accepted: 05 November 2021;
Published: 26 November 2021.
Edited and reviewed by:
Massimo Grilli, University of Genoa, ItalyCopyright © 2021 Wu, Li, Yang, Wang, Peng, Zhu, Sui, Wu, Li, Wang, Li, Zhang, Ge, Zhao and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Shao Li, lishao89@dmu.edu.cn; Jie Zhao, dlzhaoj@163.com
†These authors have contributed equally to this work