(20R)-panaxadiol improves obesity by promoting white fat beigeing

Introduction: Obesity is an important cause of a range of metabolic diseases. However, the complex mechanisms of obesity and its related diseases make some weight loss methods ineffective or have safety issues. Ginseng, a specialty of Jilin Province in China with both edible and medicinal value, contains mainly ginsenosides and other components. In order to study the anti-obesity effect of ginseng, network pharmacology was used to predict and screen the active ingredients, action targets and signaling pathways of ginseng. We found (20R)-panaxadiol (PD) is a more desirable active ingredient due to its high drug-like properties and high bioavailability. Moreover, it is closely related to cAMP pathway which is more important in metabolism regulation. The corresponding pharmacodynamic targets of PD include ADRB2 (the gene encoding the β2-adrenoceptor receptor). Our study aimed to investigate whether Panaxadiol can promote white adipocyte beigeing and increase thermogenesis through modulating the β2/cAMP pathway to exert anti-obesity effects. Methods: In vivo, we established high-fat feeding obesity model, genotypically obese mice (ob/ob) model, and administered PD (10 mg/kg). PD treatment in ob/ob mice along with β2 receptor inhibitor ICI118551. In vitro, differentiated mature 3T3-L1 cells were given palmitate (PA) to induce hypertrophy model along with PD (20 μM). Results: The results of this study demonstrated that PD significantly reduced body weight, improved glucose tolerance and lipid levels in high-fat-induced obese mice and ob/ob mice, and also reduced lipid droplet size in PA-treated hypertrophic adipocytes in vitro. Molecular biology assays confirmed that cAMP response element binding protein (CREB) phosphorylation was increased after PD administration, and the expression of thermogenesis-related proteins UCP1, PRDM16 and mitochondrial biosynthesis-related proteins PGC-1α, TFAM and NRF1 were increased. Molecular docking results showed a low binding energy between β2 receptors and PD, indicating an affinity between the β2 receptor and PD. In addition, the β2 receptor inhibition, reversed the anti-obesity effect of PD on the body weight, lipid droplets, the expression of thermogenesis-related proteins and CREB phosphorylation in ob/ob mice. Discussion: These results suggest that PD may promote the expression of thermogenic proteins through phosphorylation of CREB via β2 receptor activation, and thus exert anti-obesity effects.


Introduction
Obesity is a pathological state of fat accumulation, which is mainly manifested by the massive accumulation of fat resulting in adipocyte hypertrophy and adipose tissue hyperplasia (Zhang et al., 2017). Obesity has become a public health problem in China and worldwide, and patients suffer psychologically and physiologically problems to some extent. Some studies have shown that the incidence of diabetes is much higher in obese than normal populations, and obesity is a high risk factor for the development of cardiovascular diseases including atherosclerotic and heart failure (Maggio and Pi-Sunyer, 2003;Riobó Serván, 2013). In recent years, the role of effective active ingredients extracted from natural plants, i.e., phytochemicals, has received increasing attention in the prevention and treatment of obesity and obesity-related metabolic diseases (Azhar et al., 2016;Zhao et al., 2017). Several phytochemicals are employed to reduce the process of adipogenesis, carbohydrates absorption in the small intestine, collection of hepatic triglycerides, deposition of adipose tissue, weight loss, and enhances the anti-obesity potential, activity of PPAR-α and PPAR-γ-responsive genes (Kumar et al., 2022) (Shehadeh et al., 2021) (González-Castejón and Rodriguez-Casado, 2011). Ginseng is one of the most valuable herbs in traditional Chinese medicine (Yoo et al., 2012;Kim et al., 2017;Li and ji. 2018a;So et al., 2018;Yu et al., 2018), which contains a variety of active ingredients including ginsenosides (Ratan et al., 2021). Ginseng is a plant of high value for both food and medicinal purposes. In China, ginseng has been widely trusted and used as a medicinal herb that can make people strong and healthy (Sabouri-Rad et al., 2017). Recently, the anti-obesity effects of ginseng have been increasingly mentioned. Modern pharmacological studies have repeatedly suggested the weight loss and hypoglycemic effects of ginseng (Li and Ji, 2018b). A study by (Karu et al., 2007) showed that ginseng has some anti-obesity and hypolipidemic effects on high-fat dietinduced obese mice.
However, the bioavailability of each component of ginseng varies, and its specific mechanism of action is unclear for its multi-component and multi-target characteristics. Further study of the material basis, pharmacological efficacy and mechanism of ginseng's anti-obesity is still needed. Nowadays, the emergence of network analysis has provided new ideas to study the mechanism of action of many herbal medicines such as ginseng. In the previous study, network analysis was used to predict and screen the active ingredients, the corresponding targets and signaling pathways of ginseng. We found (20R)-panaxadiol (PD) is a more desirable active ingredient due to its high drug-like properties and high bioavailability. Moreover, it is closely related to cAMP pathway which is more important in metabolism regulation. The corresponding pharmacodynamic targets of PD include ADRB2 (the gene encoding the β 2 -adrenoceptor).
The cAMP pathway has been reported to be associated with thermogenesis in adipose tissue (Liu et al., 2019;Zhang et al., 2021). Adipose tissue can be divided into white and brown adipose. The main function of classical brown fat is to produce heat, while the function of white fat is to store energy, and both are important regulators of energy homeostasis. Recent studies have pointed to the existence of a potential cell type in white adipocytes that can specifically express PRDM16 (PRdomain-containing16), PGC-1α (Peroxisome proliferators-activated receptor-γ coactivator-1) and UCP1 (Uncoupling protein 1), biomarkers for brown-specific genes, known as beige adipocytes (Harms and Seale, 2013). Beigeing, which refers to white fat exhibiting brown fat-like properties after external environmental or pharmacological stimulation, is a phenomenon in which the expression of brown-specific genes in white fat (especially in SWAT). The cAMP signaling pathway plays an important role in the study of promoting beige coloration of white fat. Current studies have demonstrated that by activating ß-adrenergic receptors and thus adenylate cyclase, catalyzing the conversion of ATP to cAMP, activates PKA, phosphorylates the cAMP response element binding protein (CREB), and promotes downstream brown-specific genes expression, which increases energy expenditure for the purpose of disease treatment (Fenzl and Kiefer, 2014). In this study, obese mice and PAtreated hypertrophic adipocytes were used to investigate whether 20R-Panaxadiol can promote white adipocyte beigeing and increase thermogenesis through modulating the β 2 /cAMP/CREB pathway to exert anti-obesity effects.

Materials
The PD used in this study was donated by Professor Yanping Chen (College of Chemistry, Jilin University). Its purity was determined to be more than 98.9% by normalization method of HPLC. Total cholesterol (TC), triglyceride (TG) and total cholesterol (TCHO) diagnostic test kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Blood glucose test strips were purchased from Roche (Basel, Switzerland). All other reagents were purchased from Beijing Chemical Factory (Beijing, China).

Animals and treatments
The animal study designed in this paper was approved by the Medical Ethics Committee of Jilin University (protocol code 2021156, approval date 20210301). A number of C57BL/6J male mice and ob/ob male mice (3 weeks) were purchased from Beijing HFK Bioscience Co., Ltd. (SCXK 2019-0008) (Beijing, China). The mice were housed in a temperature-controlled animal room with a constant 12 h light/dark cycle. After 1 week of adaptive feeding, C57BL/6J mice were taken as control group with low fat feed [CONTROL, n = 6, Research DIETS (D12450B)], high fat group with high fat feed [n = 14, Research DIETS (D12492) ]. After 8 weeks of high-fat feeding, the mice gained 20 percent more weight than the control group were taken as obesity, and the modeling success rate was 85 percent. Then obesity mice randomly were divided into two groups, high fat group (HF, n = 6), (20R)-panaxadiol treatment group (PD, n = 6). PD was administered by gavage (10 mg/kg) for 5 weeks. CONTROL and HF group were given equivalent amounts of sodium carboxymethyl cellulose.
After 2 weeks of normal feeding, the ob/ob mice gained 20 percent more weight than the control group, were randomly divided into three groups, ob/ob group (OB, n = 6), 20R-Panaxadiol group (PD, ig. 10 mg/kg, n = 6) and PD+β 2 receptor inhibitor group (ICI118551, ip. 1 mg/kg, n = 6). Body weights of mice were measured weekly and treatments were administered by gavage or intraperitoneal injection (equivalent amounts of sodium carboxymethyl cellulose and normal saline were given as controls) from 9:00 to 10:00 a.m. daily for 8 weeks.
HF mice and ob/ob mice were subjected to oral glucose tolerance test (OGTT) at week fourth and week seventh of drug administration, respectively. Mice were anesthetized with isoflurane by inhalation (1.5%) and euthanized by CO 2 inhalation in the next week. Blood sampling was taken from the medial canthus vein and serum was collected after centrifugation at 3,000 g (4°C) for 15 min. Then serum TC, TG, TCHO were determined according to the manufacturer's instructions. Samples of subcutaneous adipose tissue (SWAT) and interscapular brown adipose tissue (BAT) were extracted for weighing.

OGTT
For the OGTT, mice were fasted for 12 h and then orally gavaged with glucose dissolved in water at 2 g/kg body weight. Ten microliters of blood was obtained from the tail tip, and the concentration of glucose was measured at 0, 30, 60, 90, and 120 min.

Hematoxylin and eosin (HE) and immunofluorescence (IF) stainings
The adipose tissue was quickly removed from the mice, washed with normal saline, dried and then fixed with 10% formalin. After rapid removal of the adipose tissue, it was placed in 4% paraformaldehyde. It was then dehydrated in an ascending series of ethanol, and equilibrated with xylene, followed by embedding in paraffin and sectioning into 5-10 µm slices. Then, the samples were dewaxed with xylene and a descending series of ethanol. Continued sections were stained with both Mayer's hematoxylin and eosin (HE).
Dried paraffin sections were dewaxed and hydrated and then closed with 1% BSA for 30 min, the closure solution was blotted dry on blotting paper. Each section was incubated with UCP1 antibody (abcam1:100) overnight at 4°C in a wet box, and washed 3 times with PBS. After aspirating the residual liquid, the sections were incubated with fluorescent secondary antibody (FITC1:100) for 1 h at room temperature in a wet box protected from light, then rinse 3 times with PBS, the excess liquid was aspirated. A small amount of antifluorescence quencher containing DAPI dropwise was added, the sample was covered with a coverslip, and stored in a wet box protected from light for observation under a fluorescence microscope for photographs.

Oil red O staining
Differentiated mature 3T3-L1 cells were given PA and PD for 24 h. The cells were gently rinsed with PBS before staining, followed by fixation with 4% paraformaldehyde for 1 h. Then cells were washed with PBS, and each well was stained with 2 mL of freshly configured Oil Red O working solution for 1-2 h. The staining solution was discarded, and 60% isopropanol was rinsed quickly once, followed by rinsing with ultrapure water three times, and observed under the microscope for photographs.

Immunoblot analysis
Proteins were extracted from cell lysates following the manufacturer's protocols (Beyotime, China). Protein concentration was quantified using the BCA protein assay kit (Thermo Fisher Scientific, United States) and 30 μg protein was separated in a 12% SDS polyacrylamide gel and electro transferred onto polyvinylidene difluoride (PVDF) membranes (Bio-Rad, United States). Membranes were blocked with 5% (w/ v) BSA for 2 h at room temperature and then incubated with primary antibodies with light shaking overnight at 4°C. Primary antibodies against UCP1, PGC-1α, TFAM, NRF1, CREB, P-CREB and GAPDH (abcam) were diluted to a ratio of 1: 1,000 in TBST buffer. The membranes were washed 3 times for 5 min each with 10 mL of TBST [10 mM Tris-HCl, 150 mM NaCl and 0.1% (v/v) Tween-20] and then incubated with secondary antibody at room temperature for 2 h. Secondary antibodies goat anti-rabbit or goat anti-mouse (Proteintech, United States) were diluted to a ratio of 1:5,000 in TBST buffer. The membrane was incubated in Western ECL substrate (Thermo fisher or Proteintech, United States) and exposed to Tanon imager, using ImageJ software for image analyses.

Statistical analysis
All data are expressed as mean ± standard error of the mean. Statistical significance of differences was analyzed by one-way ANOVA with Dunnett test using GraphPad Prism8. p < 0.05 was considered to indicate a statistically significant difference.

Anti-obesity effect of PD on HF mice
As shown in Figures 1A,B, after 5 weeks of PD administration, the body weight of mice in PD group was significantly lower than that of the model group, and the body fat ratio was significantly reduced. It is suggested that PD can reduce the body weight and body fat ratio of obese mice induced by high-fat diet. Serum TG, T-CHO and LDL (Figures 1C-E) were significantly reduced after 5 weeks of PD treatment, suggesting that PD can effectively improve the lipid levels in high-fat diet induced obese mice.

Effects of PD on glucose tolerance, fasting glucose and subcutaneous inguinal white fat morphology in HF mice
Obesity is often accompanied by varying degrees of impairment in glucose tolerance, so we used OGTT to test the effect of PD on glucose tolerance. As shown in the Figures 2A-C, after PD treatment, the AUC (Area Under Curve) of blood glucose in the PD group was lower than that of the model group, and its recovery level was significantly faster than that of the model group, and fasting blood glucose was significantly lower. The above results suggest that the elevated fasting glucose and impaired glucose tolerance in obese mice induced by high-fat diet were improved to some extent after the administration of PD. The results of HE staining of adipose tissue (Figures 2D, E) showed that after the administration of PD treatment, the volume and diameter of adipocytes were significantly reduced and the intercellular spaces were dense. It is suggested that PD has an ameliorative effect on the morphology of subcutaneous inguinal white adipose tissue in highfat diet induced obese mice.

Effect of PD on thermogenic and cAMP related pathway proteins in adipose tissue of HF mice
To investigate the anti-obesity mechanism of PD, we examined the expression of thermogenic proteins PRDM16 and UCP1, mitochondrial biosynthesis-related protein PGC-1α, TFAM and cAMP pathway related protein P-CREB in adipose tissue after (20R)-panaxadiol administration. The results are shown in the Figures 3A, B, the expression of thermogenic proteins PRDM16 and UCP1 in PD treatment mice were significantly increased in immunofluorescence (Red fluorescence represents the expression of UCP1, Figure 3C). The results were the same as the western blotting results. Mitochondrial biosynthesisrelated proteins PGC-1α, TFAM expression were also significantly increased (Figures 3D, E). In order to initially investigate the causes of the changes in adipose tissue thermogenesis and mitochondrial biosynthesis, we used Western blotting to detect the phosphorylation of the cAMP Anti-obesity effect of PD on HF mice (A, B) The changes of body weight and fat/weight (g) during the experimental course; (C-E) Serum lipid profile including circulating TG, TCHO and LDL levels; **p < 0.01, ****p < 0.0001, compared with the LF group. # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001, compared with the HF group. n = 6.
Frontiers in Pharmacology frontiersin.org 04 pathway related protein CREB, and as shown in the Figure 3F, P-CREB content increased significantly after the administration of PD. It is suggested that PD can promote thermogenesis and mitochondrial biosynthesis, and this effect may be related to the cAMP/CREB pathway.

3.4
In vitro experiments to study the ameliorative effect of PD on hypertrophic adipocytes and its association with cAMP related pathway proteins In vitro experiments were performed using MTT assay to screen PA-modeling concentration of 0.3 mMOL and PD administration concentration of 20 μMOL. The results are shown in the Figure 4C, the lipid droplet volume of PAstimulated adipocytes was increased compared with the control group. After giving PA stimulation along with PD protection, the lipid droplet volume was reduced compared with the PA group. To investigate the mechanism of action of 20R-Panaxadiol in vitro experiments, we examined the expression of the thermogenic proteins PRDM16 and UCP1, the mitochondrial biosynthesis-related protein TFAM and the cAMP pathway related protein P-CREB after administration of PD to hypertrophic adipocytes. As shown in the Figures 4D-G, PRDM16, TFAM and P-CREB expression were significantly increased after PD administration. It is suggested that at the cellular level, (20R)-panaxadiol also has the same effect of promoting thermogenesis and increasing mitochondrial biosynthesis in hypertrophic white adipocytes, and this effect may be through the cAMP/CREB pathway.

Anti-obesity effect of PD via β 2 receptor in ob/ob mice
To investigate whether the weight loss effect of 20R-Panaxadiol acts through the β 2 receptor, we administered PD on genotypically obese mice ob/ob mice, and the β 2 receptor was inhibited by β 2 receptor inhibitor (ICI118551) while PD was administered. As can be seen from Figure 5A, at week four of administration, the weight gain of the PD administered group slowed down significantly and a significant difference was observed compared to the weight of the model group. At the sixth week of administration, there was a significant difference between the body weight of the β 2 receptor inhibitor group and that of PD treatment group. There was no significant difference in the food intake of ob/ob mice in each group ( Figure 5B). As shown in Figure 5C, the lee's index increased significantly in ob/ ob mice and decreased significantly after PD treatment, while it increased significantly in the ICI118551 group compared with PD group. Figure 5D shows that the body fat ratio of ob/ob mice increased significantly compared with the control group and decreased significantly after PD treatment for 8 weeks. It was suggested that PD reduced body weight, body fat ratio and lee's index in ob/ob mice, and that β 2 receptor inhibitors reversed

FIGURE 2
Effects of PD on glucose tolerance, fasting glucose and subcutaneous inguinal white fat morphology in HF mice (A) OGTT after 4 weeks of PD treatment; (B) AUC of each group was calculated during the oral glucose tolerance test; (C) Fasting blood glucose level in each group; (D) PD alleviated morphology changes of SWAT in HF mice; (E) Cell diameter changes in each group; *p < 0.05, **p < 0.01, ****p < 0.0001, compared with the LF group. ## p < 0.01, #### p < 0.0001 compared with the HF group. n = 5.
Frontiers in Pharmacology frontiersin.org this alteration. After PD administration, serum TG and T-CHO were significantly reduced in ob/ob mice, and LDL was also reduced but without significant difference. Compared with the PD-treated group, mice in the ICI118551 group showed significant increases in TG and T-CHO ( Figures 5E-G). It was suggested that PD treatment could effectively improve lipid levels in ob/ob mice, and this effect would be diminished after β 2 receptor inhibition.

Effects of PD on glucose tolerance and subcutaneous inguinal white fat morphology in ob/ob mice via β 2 receptors
To investigate the effect of PD on glucose tolerance in genotypically obese mice, we examined the changes in glucose tolerance in mice. As can be seen from the Figures 6A, B, after PD treatment, the AUC of blood glucose in PD treatment group was lower than that of the model group, and its rate of return to normal level was significantly higher. The blood glucose of mice in the ICI118551 group was higher than that in the PD administration group. The above results suggest that glucose tolerance appears significantly impaired in ob/ob mice and is improved to some extent in ob/ob mice after the administration of PD, which may be diminished by β 2 receptor inhibitors. HE results (Figures 6C, D) showed that after PD treatment, subcutaneous inguinal white adipocytes were significantly reduced in volume and had dense cell gaps. After administration of the β 2 receptor inhibitor, the fat volume was larger compared to the PD administered group. The brown adipose fat cells in the back of PD treatment group were also significantly reduced in size, small multi-chambered lipid droplets started to appear, and the cell interstices were dense. Dorsal fat multicompartmentality

FIGURE 3
Effect of PD on thermogenic and cAMP related pathway proteins in adipose tissue of HF mice PD increased the expression of thermogenesis-related proteins in the HF group of mice. (A, C) UCP1 protein expression; (B) PRDM16 protein expression; PD increases the expression of mitochondrial biosynthesis-related proteins PGC-1α (D) and TFAM (E); (F) P-CREB content increased significantly after the administration of PD treatment; p < 0.05, compared with the LF group. # p < 0.05, ## p < 0.01, ### p < 0.001 compared with the HF group. n = 6.
Frontiers in Pharmacology frontiersin.org was reduced after administration of ICI118551. It was suggested that PD improved adipose histomorphology in genotypically obese mice and could be reversed to some extent by β 2 receptor inhibitors.
3.7 Effect of PD on adipose tissue thermogenic proteins and cAMP pathway related protein in ob/ob mice via β 2 receptors To investigate the changes in adipose tissue thermogenesis in ob/ ob mice, we used Western blotting to detect the expression of thermogenesis-related proteins PRDM16, UCP1 and mitochondrial biosynthesis-related proteins PGC-1α, TFAM, NRF1 and cAMP pathway related protein CREB in subcutaneous SWAT. The results are shown in the Figures 7A,B, the expression of heat-producing proteins PRDM16 and UCP1 significantly increased after PD treatment (Red fluorescence represents the expression of UCP1, Figure 7C). Immunofluorescence results were the same as the Western blotting results. Mitochondrial biosynthesis-related proteins PGC-1α, TFAM, NRF1 expression were also significantly increased ( Figures 7D-F). Thermogenic and mitochondrial biosynthesis-related proteins were significantly decreased in adipose tissue of mice given β 2 receptor inhibitors. To initially investigate the causes of adipose tissue thermogenesis and changes in mitochondrial biosynthesis, we used Western blotting to detect the

FIGURE 4
In vitro experiments to study the ameliorative effect of PD on hypertrophic adipocytes and its association with cAMP related pathway proteins MTT assay to screen PA-modeling concentration (A) and PD administration concentration (B); (C) Oil Red O staining; (D) PRDM16 protein expression; (E) UCP1 protein expression; PD increases the expression of mitochondrial biosynthesis-related proteins TFAM (F); (G) P-CREB content increased significantly after the administration of PD treatment; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, compared with the CONTROL group. # p < 0.05 compared with the PA group. n = 3.

Frontiers in Pharmacology
frontiersin.org phosphorylation of the cAMP pathway related protein CREB. As shown in the Figure 7G, P-CREB levels increased significantly after PD administration and decreased significantly after β 2 receptor inhibitor administration. It is suggested that the role of (20R)panaxadiol in promoting thermogenesis and mitochondrial biosynthesis may be through the cAMP/CREB pathway, and this pathway may be mediated by β 2 receptors.

Discussion
Clinical findings suggest that ginseng may improve cardiovascular function, immune function, and diabetes-related diseases (Wu et al., 2014). showed that ginsenoside Rb1 regulates appetite and achieves energy balance by modulating the expression of inflammatory factors in vivo and restoring the anorexigenic effects of leptin and leptin p-STAT3
Frontiers in Pharmacology frontiersin.org signaling in the hypothalamus in mice fed a high-fat diet. In vitro studies have shown that ginsenosides can inhibit triglyceride synthesis, cholesterol production (Lee et al., 2015), gluconeogenesis  by activating AMPK and inhibiting fatty acid synthase (FAS) and 3-hydroxy-3-methyl-glutaryl coenzyme A reductase (HMGCR). Ginseng reduced adipocyte size and fat storage in highfat diet-induced obese mice and rats (Lee et al., 2010). In conclusion, it can be seen that ginseng has certain anti-obesity effects. However, it has multi-component, multi-target and complex pathways, so there is still a long way to go to find the exact and efficient active ingredients and to elucidate their mechanisms of action. Our study found that: 1. (20R)panaxadiol has a weight loss effect on both high-fat diet-induced and genotypically obese mice. 2. (20R)-panaxadiol can regulate the cAMP pathway, phosphorylate CREB, promote white adipocyte beige, and play a role in promoting thermogenesis and anti-obesity. 3. The antiobesity effect of (20R)-panaxadiol may be due to the excitement of β 2 receptor. Activation of the cAMP pathway can phosphorylate the cAMP effector element binding protein (CREB), which in turn promotes the expression of downstream thermogenic genes such as UCP1 (Reverte-Salisa et al., 2019). Recent studies have shown that beige adipocytes present in white adipocytes can also specifically express brown-specific genes such as UCP1 induced by specific external conditions to promote thermogenesis in the organism (Wu et al., 2012). This process of conversion of white fat to beige fat is called white fat beigeing. Based on the above predictions of network analysisand the support of related literature, this project was carried out to verify the effective components and mechanism of action of ginseng against obesity by conducting in vitro and in vivo experiments. Our results suggested that PD might promote the expression of thermogenic proteins in adipocytes through the cAMP/CREB pathway and thus exert anti-obesity effects.
The well-recognized upstream target of the classical signaling pathway of cAMP is the ß receptor, and the ß receptor signaling pathway plays an important role in promoting thermogenesis and beigeing (Ohyama et al., 2016;Lim et al., 2019), of which the β 3 receptor has been studied mostly. However, it has been found that the effects of β 3 receptor agonists given in some obese populations are not significant (Redman et al., 2007;Carey AL 2013 January;56 (1)). Moreover, it has been reported that β 3 receptor expressed in obese animals and humans with impaired functional activity. For example, a variant allele of the human β 3 receptor gene (64 Trp/Arg) has been associated with inhibition of β 3 receptor signaling (Pietri-Rouxel et al., 1997;Kimura et al., 2000) and increased body mass index (BMI), then obesity was promoted (Sipilainen et al., 1997;Mitchell et al., 1998). Recently, it has been shown that β 2 receptor activation in adipocytes also promotes the activation of the cAMP/PKA pathway and exerts some anti-obesity effects. Li et al. (Li et al., 2012) showed a significant reduction Effects of PD on glucose tolerance and subcutaneous inguinal white fat morphology in ob/ob mice via β 2 receptors (A) OGTT after 7 weeks of PD treatment; (B) AUC of each group was calculated during the oral glucose tolerance test; PD alleviated morphology changes of (C) SWAT and (D) BAT in HF mice; ****p < 0.0001, compared with the CONTROL group. ## p < 0.01, compared with the ob/ob group. $ p < 0.05 compared with the PD group. n = 5.
Frontiers in Pharmacology frontiersin.org in adiposity in pigs after administration of β 2 receptor agonists, and (Ohyama et al., 2016) showed that a subtype of capsaicin could also exert some anti-obesity effects through β 2 receptors. This also suggests that the role of β 2 receptors in the ß receptor family in our anti-obesity effect needs to be looked at. After the PPI network analysis of target protein interaction, the degree of target ADRB2 (i.e., β 2 receptor coding gene) was 20, which was much higher than the average degree of 13.4. It indicated that ADRB2 may be the main pharmacological target of ginseng. In addition, the active ingredient corresponding to the target ADRB2 is also (20R)-panaxadiol. Our study subsequently used β 2 receptor inhibitor to intervene in (20R)-panaxadiol treatment of ob/ob obese mice. It was found that inhibition of β 2 receptor reversed the effect on weight loss and upregulation cAMP thermogenesis signal pathway of 20R-Panaxadiol. Based on the above theoretical calculation results and experimental validation, it can be concluded that (20R)-panaxadiol can regulate CREB phosphorylation through activating β 2 receptor and promote the improvement of body thermogenesis and the corresponding mitochondrial biosynthesis, thus achieving the anti-obesity effect. In summary, (20R)-panaxadiol, as the active component of ginseng, can exert anti-obesity effects by increasing non-tremor thermogenesis and increasing energy expenditure in the body. The mechanism of action may be to act on β 2 receptors and promote phosphorylation of cAMP response element binding protein CREB, which in turn upregulates the expression of downstream thermogenic proteins such Effect of PD on adipose tissue thermogenic and cAMP related pathway proteins in ob/ob mice via β 2 receptors PD increased the expression of thermogenesis-related proteins in the OB group of mice. (A, C) UCP1 protein expression; (B) PRDM16 protein expression; PD increases the expression of mitochondrial biosynthesis-related proteins TFAM (D), PGC-1α (E) and NRF1 (F); (G) P-CREB content increased significantly after the administration of PD treatment; *p < 0.05, **p < 0.01, ***p < 0.001, compared with the LF group. # p < 0.05, ## p < 0.01, ### p < 0.001, compared with the OB group. $ p < 0.05 compared with the PD group. n = 6.
Frontiers in Pharmacology frontiersin.org as UCP1 and PRDM16. Actually, Weight loss depends on energy expenditure of multiple organs, such as the muscle, the liver, and the adipose tissue. So we think PD may improve obesity by promoting white fat beigeing in part. In subsequent studies, we will take the studies to reveal the energy expenditure in other organs such as skeletal muscle, and on the alleviation of NAFLD. A comprehensive discussion of the effects of PD on obesity related metabolic diseases would be conducted.

Data availability statement
The datasets used and/or analysed during this study are available from the corresponding author on reasonable request.

Ethics statement
The animal study was reviewed and approved by the Institutional Animal Care and Use Committee of Jilin University (protocol code 2021156, approval date 20210301).