Mesenchymal stem cells and pulmonary fibrosis: a bibliometric and visualization analysis of literature published between 2002 and 2021

Introduction: Pulmonary fibrosis (PF) is a severe disease that can lead to respiratory failure and even death. However, currently there is no effective treatment available for patients with PF. Mesenchymal stem cells (MSCs) have been recently shown to have therapeutic potential for PF. We analyzed the literature focused of MSCs and PF to provide a comprehensive understanding of the relationship between MSCs and PF. Methods: We searched the Web of Science Core Collection database for literature from 2002 through 2021 that involved MSCs and PF. The included studies were then analyzed using CiteSpace and VOSviewers software. Results: A total of 1,457 studies were included for analysis. Our findings demonstrated the following: 1) an increasing trend of MSC and PF research; 2) among the 54 countries/regions of author affiliations, the United States was the most frequent, and the University of Michigan (n = 64, 2.8%) was the top institution; 3) Rojas Mauricio published the most articles and PLOS ONE had the most related studies; and 4) keywords, such as idiopathic pulmonary fibrosis, mesenchymal stem cells, and systemic sclerosis, were listed more than 100 times, indicating the research trend. Other common keywords, such as inflammation, myofibroblasts, fibroblasts, aging, telomerase or telomere, and extracellular matrix demonstrate research interests in the corresponding mechanisms.1) The number of publications focused on MSCs and PF research increased during the study period; 2) Among the 54 countries/regions of author affiliations, most articles were published in the United States of America, and the University of Michigan (n = 64, 2.8%) had the largest number of publications; 3) Rojas Mauricio published the most articles and PLOS ONE had the most related studies; 4) Keywords, such as idiopathic pulmonary fibrosis, MSCs, and systemic sclerosis, were listed more than 100 times, representing a research trend. Other common keywords included inflammation, myofibroblasts, fibroblasts, aging, telomerase or telomere, and extracellular matrix. Discussion: During the past 2 decades, MSCs have been proposed to play an important role in PF treatment. An increasing amount of literature focused on MSCs and PF research has been published. Our findings provide insight into the current status and research trends in the field of MSCs and PF research during the past 2 decades, which could help researchers understand necessary research directions. In the future, more preclinical and clinical studies should be conducted in this field to support the application of MSCs in the treatment of PF.


Introduction
Pulmonary fibrosis (PF) is a major type of pulmonary disease that can lead to respiratory failure and death . PF is characterized by fibroblast proliferation and extracellular matrix (ECM) aggregation, resulting in structural abnormality during aberrant repair of normal alveolar tissue injury. The clinical manifestations of PF include hypoxemia, dyspnea, respiratory failure, and even death. The etiology of PF is not specific, but there are many known contributing factors, such as autoimmune disease, viral infection, silica inhalation, long-term smoking, radiation, chemotherapy, and genetic mutations.
During the past 2 decades, significant progress has been made in understanding the pathogenesis of PF, especially idiopathic pulmonary fibrosis (IPF). IPF is the most common form of PF, and is defined as a progressive lower respiratory disease that usually affects adults over the age of 40. Alveolar epithelial dysfunction is considered a critical step in the initiation of IPF. Given that genetic factors can influence the integrity of epithelial cells, and environmental and aging-related factors can trigger epigenetic reprogramming, epithelial cells are likely to be damaged and abnormally activated by these factors (Winters et al., 2019). Activated epithelial cells secrete a large number of cytokines, such as tumor growth factor-beta (TGF-β), to promote fibroblast migration and proliferation, as well as stimulate fibroblast differentiation into myofibroblasts, which then secrete a large amount of ECM, resulting in ECM deposition (Mei et al., 2021). IPF has a poor prognosis, with an average life expectancy of 3-5 years after diagnosis without treatment (Lederer and Martinez, 2018;Raghu et al., 2018) and mortality exceeding that of many malignant tumors (Karampitsakos et al., 2017). Regardless of improvements in drug therapy, such as corticosteroids, endothelin antagonists, and pirfenidone, there is still no highly effective treatment for PF. Lung transplantation can improve the quality of life and survival of patients, but various challenges exist with this treatment, including shortage of donor organs, immune rejection, and surgical complications (Richeldi et al., 2017;Shenderov et al., 2021). Thus, relieving symptoms and delaying disease progression remain the primary management strategies . Therefore, there is an urgent need to develop new therapeutic strategies that are safe and effective for PF treatment.
Mesenchymal stem cells (MSCs) are multipotent stem cells with immunomodulatory effects and paracrine functions. MCSs can promote tissue regeneration by secreting a variety of cytokines and soluble factors with anti-inflammatory and antifibrosis properties. MSCs not only reduce the levels of inflammatory factors and the deposition of activated fibroblasts and collagen, but they can also inhibit epithelial-mesenchymal transition (EMT) and promote epithelial repair, suggesting that MSCs may be a novel therapeutic target for PF (Chambers et al., 2014;Averyanov et al., 2020;Zhao et al., 2021). As such, advances have been made in utilizing MSCs to promote repair and regeneration of lung tissue.
Bibliometrics is a type of literature analysis that allows for the quantitative and qualitative review of scientific output within a specific research field Ke et al., 2020). More specifically, bibliometrics utilizes characteristics of the literature, such as authors, keywords, journals, countries, institutions, and references related to the target research area (Ke et al., 2020), that can be analyzed and visualized using informatics tools, such as CiteSpace (Synnestvedt et al., 2005) and VoSviewer (Ke et al., 2020;Yeung et al., 2020;Teles et al., 2021;Wu et al., 2021). To the best of our knowledge, a bibliometric analysis of the literature focused on the application of MSCs to treat PF has not been performed (Zhang et al., 2020;Wu et al., 2021). Due to the urgency of developing effective therapies for PF and the potential of targeting MSCs for PF treatment, we conducted a comprehensive analysis of the scientific literature on MSCs in the treatment of PF during the past 2 decades. Our analysis provides important insights into the state of the current literature and directions for future research on MSCs and PF.

Search strategy
We conducted a search of the Web of Science Core Collection (WoSCC, https://www.webofscience.com/wos/woscc/basic-search) using the following terms: "TS = [("Stem Cell*"OR "Stromal Cell*") AND TS = ("ILD" OR "interstitial lung disease" OR "pulmonary Fibrosis")]" on 5 August 2022. Only articles and reviews published in English were selected. Other document types, such as early access, book chapters, meeting abstracts, and letters, were excluded. The details of literature selection are provided in Figure 1.

Data analysis
VOSviewer (version 1.6.18) was used to analyze the cooperation between countries and institutions, journals and co-cited journals, authors and co-cited authors, keyword co-occurrence, and network visualization map. In the map, nodes represent various items, such as country, institution, journal, and author, with node size representing the number of included items. The thickness of the lines linking different nodes reflects the degree of cooperation or cocitation of projects (Zhang et al., 2020;Wu et al., 2021).
CiteSpace (version 6.1. R1) was employed to draw the dual-map overlay of scientific journals that published articles on MSCs research in the field of PF. CiteSpace was also used to analyze references with citation bursts. Microsoft Office Excel 2019 was used to manage the data and assist the analysis.

Institution and country
The authors were distributed among 54 countries and 1,661 institutions. As shown in Table 1, the top country was the United States of America (United States) (n = 632, 30.5%), followed by China (n = 294, 14.2%), Germany (n = 124, 6.0%), Italy (n = 118, 5.7%), and Japan (n = 105, 5.1%). Literature from the United States and China accounted for almost half of all the retrieved studies. Countries with frequencies ≥6 (n = 31) were selected for visualization analysis, and a cooperation network was constructed according to the number and relationship of publications in each country/region ( Figure 3A). Positive cooperation was found between China and the United States, as well as Australia and Japan. The United States also had close collaboration with Japan, Germany, France, and Italy.
The top 10 institutions publishing on MSCs and PF were from four countries. The majority of these institutions (6/10) was in the United States. The University of Michigan (n = 64, 2.8%), University of Pittsburgh (n = 47, 2.0%), University of California Los Angeles (n = 29, 1.3%), Monash University (n = 25, 1.1%), and Harvard University (n = 24, 1.0%) were the top five institutions. Institutions with frequencies ≥13 (n = 37) were selected for visualization analysis, and a cooperation network was constructed according to the number and relationship of each institution ( Figure 3B). According to the results, there was a close association between the University of Michigan and the University of California Los Angeles, and similar findings were observed between German CTR Lung Res DZL and Helmholtz Zentrum Munchen, and the University of Michigan and Mayo Clinic. Although a large number of studies was published from Nanjing University, the collaboration of Nanjing University with other institutions was modest.

Journals and co-cited journals
The literature were published in 508 academic journals. Most studies (n = 39, 7.7%) were published in PLOS ONE, followed by    Journals with ≥8 publications were used to construct the journal network map ( Figure 4A). Among the top 15 co-cited journals, five journals had a total citation of more than 2,000 times ( Table 2). The American Journal of Respiratory and Critical Care Medicine (n = 4,307) was the most frequently cited journal, followed by Proceedings of the National Academy of Sciences (n = 2,624), the American Journal of Respiratory Cell and Molecular Biology (n = 2,411), and the Journal of Clinical Investigation (n = 2,399). Among the top 15 journals, the New England Journal of Medicine had the highest impact factor (IF = 176.079), followed by Nature (IF = 69.504). Journals with ≥500 cocitations were analyzed to determine the co-citation network ( Figure 4B). In summary, a positive co-citation relationship was observed between the American Journal of Respiratory and Critical Care Medicine and Chest, as well as Proceedings of the National Academy of Sciences and the New England Journal of Medicine.
The dual-map overlay of journals was used to analyze the relationship of citations between journals and co-cited journals (citing journals, left; cited journals, right) (Chen, 2017). The orange and green lines are the main application paths. The orange lines indicate studies published in molecular, biology, genetics, health, nursing, and medicine journals; the primary citations were in molecular, biology, and immunology journals. The green lines indicate studies published in molecular, biology, and genetics journals; the primary cited studies were in medicine, medical, and clinical journals ( Figure 5).

Authors and co-cited authors
A total of 7,842 authors were retrieved during the literature search. As shown in Table 3, the first two authors have published at least 15 studies. A collaboration network was conducted with the authors who published ≥7 papers. According to the number of relevant published studies, Rojas Mauricio, Xiaodong Han, Phan Sem H, and Weiss Daniel J had the largest nodes, and a close collaboration was observed between Rojas Mauricio and Mora Ana L, Noble Paul W and Stripp Barry R, and Henke Craig A and Bitterman Peter B ( Figure 6A).
Co-cited authors are defined as authors who are co-cited in a series of publications. Among the 43,654 co-cited authors, 11 of them were cocited >200 times. Raghu G had the highest number of co-citations (n =  Frontiers in Pharmacology frontiersin.org 06 494), followed by Khanna D (n = 373) and Ortiz La (n = 296). Authors with ≥120 co-citations were analyzed using the co-citation network graph ( Figure 6B). Many positive cooperative relationships among different co-cited authors were found, such as Khanna D and Steen VD, and Tashkin Dp and Raghu G and King Te.

Co-cited references
Co-cited references are defined as those references cited together by other publications. A total of 64,747 co-cited references were found in the publications related to MSCs and PF research. Among the top nine co-cited references related to MSCs and PF research (Table 4), each reference was co-cited ≥100 times. References with ≥60 co-citations were analyzed using the co-citation network diagram ( Figure 7A). The data showed that "Rojas M, 2005, Am J Resp Cell Mol" had active co-citation relationships with "Ortiz La, 2003, P Natl Acad Sci United States".

References with citation bursts
A citation burst refers to references that are frequently cited by scholars in a particular field over a period of time. Ten references with strong citation bursts were detected using CiteSpace, and the minimum burst duration of literature related to MSCs and PF research was 3 years. In Figure 8, the red bar represents the reference burst. The earliest citation burst occurred in 2004, and the latest occurred in 2015. Among these references, "Bone marriage-derived progenitor cells in Pulmonary Overall, the burst strength of the top 10 references ranged from 11.4 to 16.99, while duration was from 3 to 5 years. Table 5 summarizes the main research content of the 10 references with the strongest citation bursts from Figure 8.

Hotspots and Frontier research areas
The high-frequency keywords in the field of MSCs and PF research are shown in Table 6, amongst which IPF, MSCs, and systemic sclerosis appeared >100 times, representing future research directions. Other keywords, such as inflammation, myofibroblasts, fibroblasts, aging, telomerase or telomere, extracellular matrix, and epithelial-mesenchymal transition, appeared >20 times, which may point to areas of mechanistic studies involving MSCs and PF.
The cluster analysis of keywords was performed using VOSviewer software. Each circle represents a keyword, the size of the circle indicates the extent of positive correlation with the frequency of the keyword, and the line thickness linking the circles indicates the degree of correlation between these keywords. A total of four clusters were obtained ( Figure 7B), suggesting four main research directions. The yellow cluster includes systemic sclerosis, rheumatoid arthritis, interstitial lung disease, pathogenesis, and stem cell transplantation. The blue cluster

Rank
Co-cited references Citations  Friedenstein et al. (1970) in 1970, bone marrow-derived mesenchymal stem cells (BM-MSCs) have become the most common source of multipotent cells for transplantation in preclinical and clinical trials. However, harvesting BM-MSCs is a painful, invasive process and carries a risk of viral exposure. In addition, the number, differentiation potential, and maximum longevity of BM-MSCs decreases with age (Bustos et al., 2014;Cárdenes et al., 2018;Nehlin et al., 2019). Compared to BM-MSCs, umbilical cord derived mesenchymal stem cells (UC-MSCs) have a similar profile, are easier to harvest, have no ethical or safety concerns, and are more rapidly refreshed (Ding et al., 2015). At present, many preclinical studies have confirmed that MSCs are safe and effective in the treatment of PF. One meta-analysis  of 24 preclinical studies involving MSCs-targeted PF treatment showed that MSC treatment significantly improved patient survival rate and alleviated PF in animal models. All published clinical trials that have applied MSCs for PF treatment have confirmed the safety of MSC treatment and the benefits for some patients (Table 7). Of the clinical studies included in Table 7, all were collected from clinicalTrials.gov, except the fifth study (No. 5). Recently, some progress has been made in engineering MSCs to regulate and guide their behavior. For example, endocytic superparamagnetic iron oxide nanoparticles have been shown to improve MSC targeting to the lung (Silva et al., 2018).
Due to the physical properties of MSCs, exogenous engraftment of MSCs in vivo after infusion seems to be rare at the site of injury, thus hampering the therapeutic efficacy of MSCs. To avoid cellrelated problems, novel applications of MSC-derived EVs has gained interest in tissue fibrosis treatment in recent years. EVs that are 30-5,000 nm in diameter include exosomes (30-100 nm), microvesicles/ectosomes (100-1,000 nm), and apoptotic bodies (100-5,000 nm). EVs can carry a variety of substances including mRNAs, microRNAs, enzymes, and other bioactive molecules (Abraham and Krasnodembskaya, 2020;Kanai et al., 2021). An  Frontiers in Pharmacology frontiersin.org EV of endocytosis origin is called an exosome, and an EV that forms from outward budding of the plasma membrane is called a microvesicle or microparticles (Gao and Raj, 2020). MSCsderived EVs can be administered after isolation, characterization, and purification from the conditioned medium (CM) of cultured MSCs. However, there are limited studies on the effect of MSC-EVs on IPF. Mansouri and colleagues proposed that MSC-EVs can shift pro-inflammatory/classical and non-classical monocytes, as well as the alveolar macrophages, in the lung towards monocyte/ macrophage profiles, which may reduce cell apoptosis, reverse PF, and improve the Ashcroft score (Mansouri et al., 2019). The effects of MSC-EVs on lung fibroblasts were investigated by Wan et al. (2020), and the results suggested that MSC-EVs can inhibit proliferation, migration, invasion, and differentiation of fibroblasts through overexpression of miR-29-3p in IPF.
The underlying mechanisms of PF, including telomere shortening and EMT, have also been considered research hotspots. The biological process of IPF has been described as an aberrant repair response to repeated alveolar epithelial injury in genetically susceptible aging individuals. Type II alveolar epithelial cells (AEC2) serve as the primary progenitor/stem cells for alveolar regeneration during tissue repair and homeostasis (Selman and Pardo, 2006;Barkauskas et al., 2013). In IPF, AEC2 display telomere shortening, epigenetic changes, and cellular senescence, which lead to impaired renewal capacity and dysfunction. Telomeres are the protective end caps of eukaryotic chromosomes and determine the proliferative lifespan of somatic cells, functioning as the protectors of cell replication (Celtikci et al., 2021). Genetic studies have confirmed that significant telomere shortening was found in 40% of familial IPF cases and 25% of sporadic IPF cases.  The therapeutic potential of AEC type II derived from human embryonic stem cells was studied in the mouse model of bleomycininduced injury. These cells differentiated into type I pneumocytes, and abrogated the inflammatory and fibrotic response 11.41 10 SFTPC + AEC2s, as a population, function as alveolar progenitors and long-term stem cells in the adult lung 16.99 Frontiers in Pharmacology frontiersin.org  IPF, idiopathic pulmonary fibrosis; FVC, functional vital capacity; DLco, diffusing capacity of the lung for carbon monoxide; BM-MSCs, bone marrow-derived mesenchymal stem cells; IV, intravenous; AD-MSCs, adipose-derived mesenchymal stem cells; hUC-MSCs, human umbilical cord derived mesenchymal stem cells; CTD, connective tissue disorders; ILD, interstitial lung disease; ANCA, antineutrophil cytoplasmic antibodies; IPAF, idiopathic pneumonia with autoimmune features; 6MWT, 6-min walk test; CT, computed tomography.

Frontiers in Pharmacology
frontiersin.org Moreover, genetic alterations in telomere-related genes (e.g., TERT, TERC, TINF2, RTEL1, PARN, etc.) have been identified. Regulator of telomere length 1 (RTEL1), which is an essential helicase, has been implicated in sporadic IPF . Telomere shortening leads to the senescence of alveolar epithelial stem cells, and AEC2 apoptosis leads to a significant reduction in cell number, resulting in reduced alveolar regeneration . In addition, senescent AEC2 secrete high levels of interleukins, interferons, tumor necrosis factors, colony-stimulating factors, growth factors, TGF-β, and chemotactic cytokines, which promote the differentiation of fibroblasts into myofibroblasts and ongoing tissue remodeling (Zhang et al., 2022). Therefore, telomere dysfunction and AEC2 cell senescence are the main drivers of IPF (Michalski and Schwartz, 2020). Telomerase gene therapy treatment in wild-type and telomerase-deficient mice has been demonstrated to prevent the development of pulmonary profibrotic lesions (Liu et al., 2019;Piñeiro-Hermida et al., 2020). EMT plays an important role in the development of IPF as well. EMT is a process in which fully differentiated epithelial cells gradually transform into mesenchymal cells and lose their original function after exposure to extracellular factors (Nieto et al., 2016), which is an important mechanism leading to the generation of myofibroblasts. During the EMT process, epithelial cells undergo significant changes in transcriptional regulation, cytoskeleton shape, adhesion, and ECM components (Sacks et al., 2018).

Discussion
To the best of our knowledge, this is the first bibliometric study to review the research trend of MSCs in the field of PF. Our findings reveal the important discoveries, areas of research interest, and research frontiers of PF-related studies. We analyzed a total of 1,457 articles with 7,842 authors and found that during the past 2 decades, the literature involving MSCs and PF increased annually, especially after 2016. This indicates that the research on MSCs and PF is growing, and MSCs are likely to become a prominent research direction in the future treatment of PF.
Our bibliometic analysis showed that the United States (first) and China (second) were the top two countries conducting research on MSCs and PF. The top 10 institutions (60%) were from the United States, and only one institution was from China. In addition, we identified close cooperation between the top four countries (United States, China, Germany, and Italy), suggesting that there is comprehensive collaboration in the research field of MSCs and PF. Our analysis also demonstrates cooperation among several institutions. An extensive cooperation should be carried out to promote the development of MSCs research in the field of PF in China, which would facilitate a better collaboration with recognized Chinese institutes.
Most of the studies were published in PLOS ONE, and there were many papers published in journals with high impact factor, including the American Journal of Respiratory and Critical Care Medicine (IF = 30.528, Q1) and Journal of Clinical Investigation (IF = 19.456, Q1). Regarding co-cited academic journals, most studies were from journals with high impact factors (Q1 region). These journals have a significant international influence and could determine the future direction of MSCs and PF research.
Current studies that have investigated the role of MSCs in PF were mainly published in journals focused on the topics of molecular biology and immunology, and a small number was published in medical journals. This indicates that the application of MSCs remains largely at the level of bench research, and only several studies have entered translational stages. Therefore, the utilization of MSCs in the management of PF may be clinically available soon.
The most common keywords, including IPF, MSCs and systemic sclerosis, and others described previously represent the current research direction and research interests. Importantly, the efficacy and safety of MSCs for the treatment of PF have been confirmed in animal studies (Harrell et al., 2019;Yang et al., 2021;Zhao et al., 2021). Further cluster analysis of keywords showed that the role of MSCs in the management of PF may be explained by the following mechanisms: 1) Various growth factors, including keratinocyte growth factor, hepatocyte growth factor, epidermal growth factor, and angiogenic factors secreted by MSCs, lead to re-epithelialization and angiogenesis (Cahill et al., 2016;Lan et al., 2017;Li et al., 2017); 2) MSCs could express a series of receptors, intercellular contact molecules, and soluble factors [e.g., Toll-like receptors (Shirjang et al., 2017)] that regulate the adaptive and innate immune system by inhibiting the maturation of T-cells and dendritic cells, reducing the activation and proliferation of B cells, and inhibiting the cytotoxicity of natural killer cells (Ni et al., 2018;de Castro et al., 2019;He et al., 2020); 3) MSCs exert an immunosuppressive effect by increasing the level of interleukin (IL)-10 secreting regulatory B cells. In addition, MSCs could release a series of anti-inflammatory cytokines and chemokines, such as IL-1 receptor antagonist (Harrell et al., 2020), IL-4, IL-10, interferon (IFN)-γ, and prostaglandin (PGE)-2; 4) MSCs could reduce the proportion of cells with a profibrotic phenotype (Type 2 macrophages) to exert antifibrotic effects (Willis et al., 2018). Moreover, MSCs can reduce the content of collagen fibers and inhibit lung refactoring through adjusting the proportion of metalloproteinases/metal protease inhibitors, thus antagonizing fibrosis (Xu et al., 2017;Chu et al., 2019); 5) MSCs can interact with endothelial cells and epithelial cells, inhibit EMT, and promote angiogenesis and alveolar repair (Wei et al., 2021).
As reported in previous human studies, the injection of stem cells is safe in practice. Although the positive role of MSC therapy has been acknowledged in some literature, the efficacy of cell therapy varies (Campo et al., 2021) (Averyanov et al., 2020). In most studies, autologous or allogeneic bone marrow MSCs with different doses ranging from 1 to 200 × 10 6 cells were administrated via different routes (Tzouvelekis et al., 2013;Glassberg et al., 2017;Averyanov et al., 2020;Campo et al., 2021). To date, most clinical studies remain in the early stage, and stem cell therapy still needs to be evaluated for its safety, feasibility, tolerability, and efficacy. It should be noted that limitations exist widely in these studies, including small sample size, lack of randomization, and/or placebo control. Furthermore, stem cell therapy requires optimization, including the type of stem cells, in vitro modifications, intervention protocol, route of administration, and dosage (Saleh et al., 2022). EVs and functional enhanced MSCs, such as those overexpressing CXCR4  and CXCR7 (Shao et al., 2019) or those with endocytosis of superparamagnetic iron oxide nanoparticles (Silva et al., 2018), have better therapeutic effects and may become a hot spot in the field of MSCs research in the future. Overall, current studies support that MSC therapy is safe for PF treatment and some Frontiers in Pharmacology frontiersin.org patients could benefit from the therapy. However, since IPF is a disease with great heterogeneity, it is critical to identify subgroups that can benefit from stem cell therapy.
In this study, we systematically analyzed the literature related to the role of MSCs in the management of PF using a bibliometrics approach. Our analysis provides comprehensive insight for clinicians and scholars in the research field of MSCs and PF. Scientometrics provides a descriptive and quantitative analysis and yields more evidence on the status of the research field compared to the traditional narrative commentary. When interpreting our results, several limitations should be taken into consideration. For example, only one database (WoSCC) was searched, only literature published in English was included, and only literature published in the past 2 decades was included. These limits could lead to potential bias.
Overall, our findings provide insight into the status and research trends in the field of MSC and PF during the past 2 decades. Our findings could help guide future preclinical and clinical studies on the application of MSCs in the treatment of PF.

Data availability statement
The raw data supporting the conclusion of this article will be made available by the authors, without undue reservation.

Author contributions
YL, ZN, ZZ, and YZ analyzed the data and created the visualizations. YY and YC wrote the manuscript. LZ and KX reviewed and revised the manuscript. All authors contributed to the article and approved the submitted version.

Funding
This work was supported by Natural Science Foundation of Shanxi Province [grant number 202203021212104].