Pre-clinical toxicity assessment of Artemisia absinthium extract-loaded polymeric nanoparticles associated with their oral administration

Background: This study was designed to quantify the composition of the ethanolic extract of Artemisia absinthium through gas chromatography–mass spectrometry analysis and ensure in vivo safety of A. absinthium extract-loaded polymeric nanoparticles (ANPs) before considering their application as a drug carrier via the oral route. Methods: We synthesized N-isopropylacrylamide, N-vinyl pyrrolidone, and acrylic acid crosslinked polymeric NPs by free-radical polymerization reaction and characterized them by Fourier-transform infrared spectroscopy, transmission electron microscopy, and dynamic light scattering spectroscopy. Different concentrations of extract (50 mg/kg, 300 mg/kg, and 2,000 mg/kg body weight) were encapsulated into the hydrophobic core of polymeric micelles for the assessment of acute oral toxicity and their LD50 cut-off value as per the test procedure of OECD guideline 423. Orally administered female Wistar rats were observed for general appearance, behavioral changes, and mortality for the first 30 min, 4 h, 24 h, and then, daily once for 14 days. Result: ANPs at the dose of 300 mg/kg body weight were used as an initial dose, and rats showed few short-lived signs of toxicity, with few histological alterations in the kidney and intestine. Based on these observations, the next set of rats were treated at a lower dose of 50 mg/kg and a higher dose of 2,000 mg/kg ANPs. Rats administered with 50 mg/kg ANPs remained normal throughout the study with insignificant histological disintegration; however, rats treated at 2,000 mg/kg ANPs showed some signs of toxicity followed by mortality among all three rats within 24–36 h, affecting the intestine, liver, and kidney. There were no significant differences in hematological and biochemical parameters among rats treated at 50 mg/kg and 300 mg/kg ANPs. Conclusion: We conclude that the LD50 cut-off value of these ANPs will be 500 mg/kg extract loaded in polymeric NPs.

. Copolymerization of the acrylic acid provides a carboxylic group, which makes it pH-sensitive and responsive when the pH is changed at the particular cell or tissue in the body. Similarly, NIPAAM, being a thermoresponsive polymer, has been known to have lower critical solution temperature (LCST) (32°C) near physiological temperature, and its copolymerization increases its LCST above which its polymeric form collapsed. The anticancer therapeutic potential of A. absinthium ethanolic extract-loaded NIPAAM-VP-AA NPs against breast cancer cell lines (MCF-7 and MDA MB-231) was previously evaluated by our research team (Mughees et al., 2020). The tumor microenvironment (leaky vasculature with irregular epithelial cells, elevated temperature around cancerous cells due to inflammation, and low physiological pH due to an acidic environment) was utilized for the targeted drug delivery system employing pH and thermoresponsive polymeric nanoparticles for in vitro breast cancer treatment.
The A. absinthium extract in conjugation with NIPAAM-VP-AA NPs would aid the establishment of potential drug with enhanced bioavailability, retention time, and efficacy of the extract due to site-specific drug delivery. The in vivo behavior of these NPs and their capability to cross certain biological barriers after oral administration have not been scrutinized previously. The oral utilization of nanoformulations with a larger surface-area-tovolume ratio provides greater surface area for mucosal interaction and adherence, protecting entrapped therapeutic agents from extreme pH conditions and enzymatic degradation and facilitating sustained release and enhanced systemic absorption of drugs or intact NPs in the gastrointestinal (GI) tract. However, the fate of NPs in the GI tract and further distribution throughout the body depends on their size, shape, composition, surface charge, conformation, and concentration (Peppas and Kavimandan, 2006;Hua, 2020). As inadequate information is available in the scientific literature about in vivo oral administration of the A. absinthium ethanolic extract and NIPAAM-VP-AA polymeric NPs, thus, the investigation of the in vivo safety of A. absinthium ethanolic extract-loaded NIPAAM-VP-AA NPs (ANPs) is a prerequisite before considering their clinical implication as a drug carrier via the oral route.
The main objectives of the present study were to identify the composition of the ethanolic extract of A. absinthium grown in the Indian subcontinent and the evaluation of the acute oral toxicity of these A. absinthium extract-loaded polymeric nanoparticles in rats as per the OECD guidelines for the Testing of Chemicals (423), Acute Oral Toxicity-Acute Toxic Class Method. This guideline will also allow the determination of the LD50 cut-off value of these ANPs and their further ranking for classification purposes and hazard assessment.
2 Materials and methods 2.1 Reagents which was freshly distilled before use. Acrylic acid (AA) and N, N′methylenebisacrylamide (MBA) were procured from Sigma-Aldrich (United States). Ferrous ammonium sulfate (FAS) and ammonium persulfate (APS) were purchased from SRL Pvt. Ltd. and Hi-media. Absolute ethanol was purchased from Merck (Germany).

Plant material and preparation of the ethanolic extract
Plant A. absinthium (vegetative stage) was obtained during midsummer from Jamia Hamdard Herbal Garden. It was thoroughly washed to remove dust or dirt, and its different parts (root, leaf, and aerial portion) were shade-dried. Whole plant extract synthesis was carried out through maceration of 5 gm dried powdered sample mixed with 20 mL ethanol (1:5 = drug:solvent ratio) at 28°C ± 2°C for 24 h in an incubator shaker. This procedure was repeated three times consecutively, and the final collected solvent was filtered through Whatman filter paper no. 45. A rotary evaporator was used to concentrate the filtrate under vacuum pressure at 40°C until 5 mL solvent was left (with extraction yield = 3.06%), which was further filtered through a 0.22 µm Millipore syringe filter and stored at 4°C for further use.

Gas chromatography-mass spectrometry analysis
The types of active compounds present in the whole plant ethanolic extract of A. absinthium grown in the Indian subcontinent have not been reported previously. Therefore, it needs to be addressed before their therapeutic application. Thus, the separation and identification of compounds in the A. absinthium ethanolic extract were performed using a GC/MS QP 2010 (Shimadzu) equipped with an auto-injector (AOC-20i + s) mass selective detector having an ion source temperature of 220°C, interface temperature of 270°C, solvent cut time of 3.50 min, threshold of 1,000 eV, and mass range of 40-650 m/z. Compounds were separated using an Rxi-5 Sil MS capillary column (Restek Company, Bellefonte, United States) having dimensions 30 m × 0.25 mm × 0.25 μm (film thickness). The split injection mode was used at a split ratio of 10:1 having an injection temperature of 260°C. 1.0 μL of ethanolic extract was fixed as the injected volume. Oven temperature program started from 80°C (3 min) and further increased to 300°C at a rate of 10°C/min (16 min hold). Helium was used as the carrier gas at a linear flow velocity of 40.5 cm/s with a total and column flow fixed at 16.3 and 1.21 mL/min, respectively. Compounds were identified by the comparison of their relative retention time and mass spectra with those in the NIST libraries and literature data.

Synthesis and characterization of polymeric nanoparticles
Polymeric nanoparticles were synthesized as described previously (Mughees et al., 2020). Monomeric NIPAAM, VP, and AA were used in the molar ratio of 90:10:5. This protocol is based on the free-radical mechanism where MBA was used for crosslinking the monomers, FAS was used as an activator, and APS as an initiator in the polymerization reaction. Then, 180 mg NIPAAM, 20 µL VP, and 10 µL AA were dissolved in 20 mL double distilled water with vigorous vortexing. For crosslinking the monomers, 100 µL MBA (0.049 g/mL) was added and nitrogen gas was passed for 1 h to remove dissolved oxygen from the reaction. Then, 60 µL FAS (5 mg/mL) and 100 µL APS (saturated) were added in the reaction to trigger polymerization that lasted for 24 h at 32°C under nitrogen atmosphere. After completion, the final solution was dialyzed through a cellulose dialyzing membrane (cut-off 12 kDa) and lyophilized for further use.
Synthesized NPs were characterized for their average size distribution and polydispersity index (PDI) by Zetasizer Nano ZS Ver. 7.13, (Malvern Instruments Ltd., Worcestershire, United Kingdom) through dynamic scattering light (DLS) spectroscopy. 2 mL aqueous solution was used for measurement employing a laser beam (wavelength of 633 nm) passing through the solution at 25°C with a detection angle of 90°.
Transmission electron microscopy (TEM) was performed on Tecnai G20 HR-TEM (Thermo Scientific) operated at a voltage of 200 kV to analyze the size and morphology of synthesized NPs. A drop of NPs was placed on a carbon-coated copper grid, and 2% uranyl acetate was added. The grid was air-dried, and TEM images were obtained through a high-resolution digital CCD camera with image processing software (Olympus Soft Imaging System, Germany).
Fourier-transform infrared (FTIR) spectra of NIPAAM, VP, and AA and synthesized NPs were recorded through a Varian 7000 FTIR Spectrometer with a Varian 600 UMA Microscope using the KBr pellet method. Each monomer and NPs were scanned at wavenumbers in the range of 4,000 cm −1 to 400 cm −1 with a resolution of 2 cm −1 and 16 scans per sample.

Animals
Twenty-one 8-12-week-old (100-150 gm) healthy female Wistar rats were procured from the Central Animal House Facility (CAHF), Jamia Hamdard, New Delhi, and randomly housed in a group of three per cage under standard conditions (20 ± 2°C; 50 ± 10% relative humidity; and 12 h of light/dark cycles) for acclimatization to ideal laboratory conditions 7 days before the start of the experiment. The rats were provided free access to food and water ad libitum. All the experiments were approved and performed in compliance with the Institutional Animal Ethics Committee (IAEC) constituted through the Committee for the Purpose of Control and Supervision of Experiments on Animals (173/GO/Re/S/2000/CPCSEA) under the Ministry of Animal Welfare Division, Government of India, New Delhi.

Acute oral toxicity study
The toxicological assessment was conducted according to the procedures of the Organization for Economic Co-operation and Frontiers in Pharmacology frontiersin.org 03 Development (OECD) guideline for the Testing of Chemicals (423), Acute Oral Toxicity-Acute Toxic Class Method, which is a (single dose) 14-day acute oral study using three female Wistar rats per step. The range of acute toxicity of the test substance depends on the mortality incidence of the animals which assists in finding the toxicity category of the tested drug defined by fixed LD50 cut-off values. For animal welfare reasons, the OECD guideline 423 recommends using 300 mg/kg body weight as a starting dose, when sufficient information is not available about the substance to be tested. Lyophilized NPs were dissolved in distilled water, and the extract was added slowly with continuous vortexing and mild sonication to facilitate its physical entrapment inside the hydrophobic core of NPs. Different concentrations of A. absinthium ethanolic extract were loaded into NPs as per the body weight of each rat, and food but not water was withheld overnight before dosing. Group CN (n = 6) served as the control that received distilled water via oral gavage, while group ANP 300 (n = 6) received A. absinthium ethanolic extract-loaded NPs at an initial dose of 300 mg/kg body weight. The rats were critically observed for general appearance, behavioral changes, and mortality rate for the first 30 min, 4 h, 24 h, and then, daily once for 14 days. The body weight of each rat was monitored and recorded periodically throughout the study.
Based on the absence or presence of mortality, group ANP 2000 (n = 3) received the next higher dose of 2,000 mg/kg body weight ANPs. With a minor modification to the OECD guideline 423 due to the presence of few toxic signs among ANP 300 -treated animals, another group ANP 50 (n = 6) received the next lower dose of 50 mg/kg body weight ANPs. On the 15th day, blood was drawn from the tail vein in EDTA-coated sterilized vials and anticoagulant free vials for whole blood and serum collection, respectively. The rats were euthanized by CO 2 inhalation for procuring all the vital organs (stomach, intestine liver, kidney, spleen, heart, and brain) for necropsy, macroscopic examination, and histopathological analysis.

Histopathology
Various vital organs (stomach, intestine liver, kidney, spleen, heart, and brain) were fixed in a 10% buffered formalin solution before embedding in paraffin wax. From these blocks, 3-4 μm sections were made and stained with hematoxylin and eosin (H&E). Histopathology was carried out to identify any morphological and degenerative changes in different organs due to ANP administration.

Statistical analysis
The data obtained from all the groups were statistically analyzed by GraphPad Prism Version 7 and expressed as the mean ± standard deviation. Differences in hematology and biochemistry data were analyzed using one-way ANOVA followed by Dunnett's multiple comparison tests. However, body weight results were analyzed using two-way ANOVA followed by Dunnett's multiple comparison tests, and p-value < 0.05 was considered statistically significant.

Synthesis and characterization of the synthesized polymeric nanoparticles
The free-radical polymerization process was exploited for the copolymerization of NIPAAM, VP, and AA monomers resulting in the formation of amphiphilic micelles with an external hydrophilic shell and an inner hydrophobic core. This hydrophobic core of the micelles was utilized as the carrier of the plant extract. The average particle size of NPs was found to be 131 nm with PDI 0.2 at 25°C as determined by DLS, indicating monodispersity in solution [ Figure 2I]. TEM analysis revealed prominent spherical-shaped morphology of NPs with an average size of 117 nm ± 4.04 nm as shown in Figure 2II. FTIR spectra of monomeric NIPAAM, VP, AA, and synthesized polymeric NPs are shown in Figure 2III. FTIR spectra of NIPAAM [ Figure 2IIIa] show characteristic absorption peaks at 3,297 cm −1 corresponding to N-H stretching vibrations of the secondary amide group. Absorption peaks in the range of 2,970-2,875 cm −1 correspond to C-H bond stretching vibrations of methyl and isopropyl groups in NIPAAM. The absorption peak at 1,659 cm −1 and 1,548 cm −1 occurred due to amide I-associated C=O stretching vibrations and amide II-associated N-H bending with C-N stretching vibrations, respectively. A medium-intensity absorption peak at 1,620 cm −1 originated from the stretching vibrations of the C=C bond, while peaks in the range of 963-809 cm −1 appeared due to the out-of-plane bending vibrations of = C-H bond in the vinyl group of NIPAAM. Other less-intense peaks in the range of 1,369-1,307 cm −1 correspond to vibrations from the isopropyl group of NIPAAM. FTIR spectra of VP [ Figure 2IIIb] had characteristic absorption peaks at 3,484 cm -1 and 1,711 cm −1 occurring due to O-H and amide C=O stretching vibrations, respectively. Absorption peaks in the range 2,985-2,885 cm −1 and 1,634 cm −1 correspond to C-H and C=C stretching vibrations, respectively. Peaks at 1,420 cm −1 and 1,460 cm −1 occurred due to = C-H and aromatic ring stretching vibrations. FTIR spectra of AA [ Figure 2IIIc] represent characteristic absorption peaks at 3,074 cm −1 and 1,704 cm −1 due to carbonyl O-H bond and C=O bond stretching vibrations, respectively. Additionally, absorption peaks at 1,636 cm −1 and 985 cm −1 are designated to C=C and = C-H stretching vibrations, respectively. However, FTIR spectra of synthesized polymeric NPs [ Figure 2IIId] depict changes in the peaks corresponding to specific functional groups in monomers indicating the formation of new interactions. There were no peaks in the range of 800-1,000 cm −1 corresponding to stretching vibrations of vinyl double bond indicating that polymerization has occurred among monomers via breaking of the C=C bond (Larrañaga et al., 2011;Verma et al., 2016). Absorption peaks at 1,639 cm −1 and 1,720 cm −1 correspond to C=O stretching vibrations from all three monomeric units in the polymer. Another broad and intense peak at 3,445 cm −1 represents O-H stretching vibration due to attached water of hydration with the polymer (Gupta et al., 2000;Mughees et al., 2020). In comparison with the monomer, declination in the absorption peaks in the range of 1,369-1,307 cm −1 corresponding to vibrations from the isopropyl group implies their involvement as the crosslinking points.

Acute oral toxicity study
All rats were observed individually for several clinical signs of toxicity including loss of appetite, skin and fur change, sitting at the corner, mortality, convulsion, drowsiness, salivation, respiratory depression/irregular respiratory pattern, lacrimation, abnormal sleeping pattern, tremors, diarrhea, aggressiveness, and piloerection and recorded as shown in Table 3. Group ANP 300 treated at an initial dose of 300 mg/kg appeared less active and drowsy, sat at the corners of the cage with loss of appetite for the first 30 min of the oral administration compared to control, and then, appeared normal afterward. The presence of these few clinical signs but no mortality in animals treated at 300 mg/kg led us to proceed to the next higher dose of 2,000 mg/kg and the next lower dose of 50 mg/kg. Group ANP 2000 showed few signs of toxicity in the first 30 min which included loss of appetite, sitting at the corner, and drowsiness.  3,3a,4,6,8,9,10,10a,11,12,12a,12b-tetradecahydrobenzo[4,5] Group ANP 2000 animals were excluded as major toxicity signs appeared after 4 h of dosing that included lacrimation, diarrhea, aggressiveness, respiratory depression, and abnormal sleeping pattern, and all three animals died within 24-36 h. Macroscopic examination of organs dissected from dead animals did not reveal any necrosis, hemorrhage, or changes in size, shape, and color. Group ANP 50 remains active and normal without showing any adverse signs of toxicity. As per the principle of the OECD guideline 423 shown in "Annexure 2c," for all three animals dying at 2,000 mg/kg dose within 24-48 h, while all the animals surviving at 300 mg/kg dose during the study, the LD50 cut-off value of ANPs will be 500 mg/kg body weight extract loaded in polymeric NPs.

Histopathology
Microscopic histopathological observations of the intestine, liver, and kidney of all the groups are shown in Figure 4. Figure 4A shows a normal intestinal architecture of group CN, and Figure 4B shows the normal intestinal architecture of group ANP 50 with insignificant inflammatory cell infiltrate. Figure 4C shows minor ulceration and inflammatory cell infiltrate in the lamina propria, and Figure 4D shows ulceration of intestinal mucosa with dense mixed inflammatory infiltrates in the lamina propria of group ANP 300 and ANP 2000 , respectively. Necropsy analysis of animals treated at 2,000 mg/kg ANPs reveals that a small amount of ANPs was deposited in the lumen of the intestine. These degenerative and corrosive effects of ANPs at intestinal pH can be attributed to the fact that ANPs were able to pass through the intestinal epithelium and reached the lamina propria. Representative photomicrographs of the liver [Figures 4E-G] from groups CN, ANP 50 , and ANP 300 represent the preserved hepatic lobular architecture, normal cell plate thickness, and polarity of hepatic parenchyma without necrosis, biliary cell damage, or inflammatory infiltrate. However, dilatation of sinusoids and a few central veins with interspersed congested blood vessels without necrosis or inflammatory infiltrate were seen in all the groups which could be due to the impaired venous drainage at the time of death. Figures 4I-L belong to sections from the kidney comprising of the cortex and medulla of groups CN, ANP 50 , ANP 300 , and ANP 2000 showing normal histological features of renal tubules and glomeruli with no evidence of ischemia or necrosis. However, Figure 4J of ANP 50 shows focal areas of the cortex with inflammatory infiltrates. Figures 4K, L shows focal areas of hemorrhage in the renal cortex without any other architectural distortion in animals treated at 300 mg/kg and 2,000 mg/kg, respectively. After entering systemic circulation, the kidney could be the major site of action for these ANPs where they would have accumulated causing renal toxicity.
Histological changes were not observed in the stomach, heart, brain, and spleen of treated groups as compared to the control. The stomach and heart sections from groups CN, ANP 50 , ANP 300 , and ANP 2000 showed the normally arranged gastric architecture and maintained polarity of myocytes arranged in muscle bundles [ Figures 5A-H]. The brain from groups CN, ANP 50 , ANP 300 , and ANP 2000 did not suffer any alteration and had normally arranged astrocytes, oligodendrocytes, axons, and interspersed capillaries in a fibrillary background [ Figures    Loss of appetite

Skin and fur change
- Sitting at the corner

Hematological and biochemical parameters
All the hematological and biochemical parameters for Group CN, ANP 50 , and ANP 300 were under normal physiological range and did not show any significant differences compared with the control group as shown in Table 4. As all the parameters were normal, no metabolic perturbations caused by these prolonged circulating ANPs were perceived in the study. Analysis of hematological and biochemical parameters for group ANP 2000 could not be carried out as all animals succumbed to the highest dose.

Discussion
The GC-MS analysis of the A. absinthium ethanolic extract revealed forty-five well-known terpenoids (alpha-curcumene, reynosin, phytol, isogeraniol, squalene, cycloartenol, and 24methylenecycloartenol), phytosterols (campesterol, stigmasta-5,22-dien-3-ol, and gamma-sitosterol), flavonoids (Artemetin), and lignan (yangambin and sesartemin) that have promising antioxidant, anti-inflammatory, antimicrobial, anticancer, and antiviral properties, attributing to the medicinal potency of the plant (Gonzalez-Burgos and Gómez-Serranillos, 2012). There is no previously available information about the toxicity assessment of a single oral dose of A. absinthium extract-loaded NIPAAM-VP-AA polymeric NPs that can cause severe complications in rodents. In this study, we first synthesized NIPAAM-VP-AA polymeric NPs by the free-radical polymerization reaction which was further confirmed by TEM, DLS, and FTIR spectroscopy. Different concentrations of the ethanolic extract of A. absinthium were encapsulated into the hydrophobic core of polymeric micelles for their acute toxicological assessment as per the OECD guideline 423.
NIPAAM-VP-AA NPs loaded with a variety of therapeutic compounds (e.g., ketorolac, riluzole, and curcumene) were administered via different routes, namely, ocular, intraperitoneal, and intranasal (Gupta et al., 2000;Bisht et al., 2010;Ahmad et al., 2013;Verma et al., 2016). This is the first study, in our knowledge, that was engaged in the evaluation of the LD50 cut-off value and exposure range of these polymeric ANPs for oral administration. Different studies have reported varying LD50 values of the A. absinthium alcoholic extract, i.e., 2,499 mg/kg, 3,700 mg/kg, and more than 5,000 mg/kg b.wt. (Parra et al., 2001;Mahmoudi et al., 2009;Daradka et al., 2014). As per our observations and the test procedure of the OECD guideline 423, ANPs exhibit dosedependent toxicity as all three animals died at the highest dose of 2,000 mg/kg, and their LD50 cut-off value will be 500 mg/kg body weight. According to the Globally Harmonized System, the substance having LD50 > 300-2,000 belongs to "category 4." Thus, the evaluation of obtained data enabled us to conclude that A. absinthium extract-loaded NIPAAM-VP-AA polymeric NPs lie under Globally Harmonized System (GHS) "category 4," i.e., moderately toxic. A subacute toxicity study demonstrated that orally administered poly (N-isopropyl acrylamide) copolymerized with acrylic acid (PNIPAAm-co-AAc) did not cause any toxicity in mice up to 2,000 mg/kg (Malonne et al., 2005). Therefore, we anticipated the involvement of the A. absinthium ethanolic extract to be responsible for the toxicity caused by ANPs at 2,000 mg/kg.
Modifications that occurred in the animal body weight during toxicity studies are reliable predictors of metabolic unfavorable effects of the test substance under investigation (da Silva Oliveira et al., 2016). There was a gradual weight gain in control as well as in animals treated at 50 mg/kg throughout the study. However, the body weight of animals treated at 300 mg/kg ANPs remained quite stagnant for up to 1 week, which significantly increased during the next week. Moreover, mild intestinal inflammation was also found in these animals, which corroborates our result, indicating that 300 mg/kg ANPs could compromise nutrient absorption and subsequent weight gain (da Silva Oliveira et al., 2018). However, this pathological and metabolic perturbation was short-lived and reversible. Similarly, behavioral symptoms of toxicity including drowsiness and loss of appetite, which were observed among these animals, also persisted for a short period. Meanwhile, there were no adverse clinical signs observed in animals treated at 50 mg/kg ANPs.

FIGURE 3
Increase in the mean value of body weight among control and treated groups. Significant increase in body weight was noted in control and groups treated at 50 mg/kg throughout 14 days. No significant growth was noticed in animals treated at 300 mg/kg till the 7th day, although weight gain resumed and continued till the 14th day. Values are expressed as the mean ± SD (N = 3); *p < 0.05 compared to the control.
Frontiers in Pharmacology frontiersin.org 11 Analysis of the in vivo hemocompatibility of ANPs and their interaction with the cellular components of blood, namely, erythrocytes, platelets, and leukocytes, is a prerequisite to ensure its safety for effective translation to clinical implication. Assessment of hematological and biochemical parameters is crucial to monitor the toxicity profile of different chemicals which was carried out in our study to evaluate the effect of these prolonged circulating ANPs on the hematopoietic system. The hematopoietic system is extremely sensitive to toxic compounds and serves as an indicator of the physiological and pathological status of animals in toxicological studies (da Silva Oliveira et al., 2016). Once entering system circulation, polymeric micelles of amphiphilic nature with the hydrophilic outer shell (>200 nm) possess prolonged circulating time and biocompatibility by evading reticuloendothelial systems (RESs) (Liu et al., 2005;Ashraf et al., 2018). Previously, 400 nm-sized NIPAAM-VP-AA NPs showed high cytotoxicity and apoptosis in the murine macrophage cell line J774 compared to 100 nm-sized NPs, confirming macrophage activation and recruitment of the inflammatory cascade (Ashraf et al., 2018). In our study, there were no significant differences in hematological parameters among animals treated at the dose of 50 mg/kg and 300 mg/kg ANPs as compared to control after 14 days. However, there was an apparent treatment-related insignificant increase in the total leukocyte count (TLC) and platelet count in animals treated at the dose of 50 mg/kg and 300 mg/kg after 14 days of dosing. Increment in TLC indicates the activation of the immune system of animals either due to stimulated or disturbed lymphopoiesis (Çetin et al., 2010;Otuechere et al., 2014). The finding of our study with minor increment in TLC and platelet count suggests immunomodulatory and wound healing properties of A. absinthium ,000 mg/kg represents maintained lobular architecture, normal cell plate thickness, and polarity of hepatic parenchyma without necrosis, biliary cell damage or inflammatory infiltrate; although dilatation of sinusoids (Blue arrow) and few central veins with interspersed congested blood vessels (Green arrow) were also seen in all the groups (Blue arrow indicate dilated sinusoids and green arrow indicate congested central veins). Sections of Group (I) CN (control), (J) ANP 50 treated at 50 mg/kg, (K) ANP 300 treated at 300 mg/kg, and (L) ANP 2000 treated at 2,000 mg/kg represent the kidney comprising of cortex and medulla where cortex shows numerous normal glomeruli having normal capillary loops and normal mesangial matrix deposition, normal proximal convoluted tubules, distal convoluted tubules, the loop of Henle and interstitium without any area of ischemia or necrosis; although (J) had focal areas of renal cortex with mild inflammatory infiltrate (Black arrow); (K) and (L) had focal areas of hemorrhage in the cortex (White arrow) (White arrow depict haemorrhage and black arrow depict inflammatory infiltrates).
Frontiers in Pharmacology frontiersin.org 12 extract-loaded NPs at lower doses (Yamthe et al., 2012), which is in agreement with the previous study of Amat et al. that demonstrated immunomodulatory effects of A. absinthium extracts in mice indicating toward its antiradical and antioxidant activity (Amat et al., 2010). Therefore, we suggest that ANPs with 100-200 nm hydrodynamic size are hemocompatible and do not cause lethal alterations in hematological parameters at a single low dose. Liverand kidney-related biochemical enzymes are reliable indicators of possible toxicity caused by exposure to different drugs (Farag et al., 2006). All the liver-and kidney-related enzymes, as well as electrolyte levels, were normal in animals treated at the dose of 50 mg/kg and 300 mg/kg ANPs. Therefore, we suggest that neither metabolic disturbance nor renal dysfunction was caused by ANPs at low doses in the rats. However, the impact of 2,000 mg/kg ANPs on hematological and biochemical parameters could not be inferred as all the animals died during the experiment.
In our study, histological evidence reveals the toxic potential of ANPs mainly in the intestine and kidney. Orally administered small NPs (50-100 nm) can move through the intestinal epithelium to lamina propria of the GI tract via a transcellular pathway comprising enterocytes to finally enter the systemic circulation, while large NPs (>100 nm) are taken up by M cells (Pridgen et al., 2015;Reinholz et al., 2018). It is also reported that NPs may experience direct and indirect movement in systemic circulation through the intestinal lymphatic system and hepatic portal system, respectively (Hua, 2020). We observed that a single oral dose of ANPs at 300 mg/kg caused mild intestinal inflammation, while 2,000 mg/kg caused ulceration of intestinal mucosa and inflammatory response in the Histological sections of the stomach, heart, brain, and spleen of rats (hematoxylin and eosin stain, magnification ×400). Sections of group (A) CN (control), (B) ANP 50 treated at 50 mg/kg, (C) ANP 300 treated at 300 mg/kg, and (D) ANP 2000 treated at 2,000 mg/kg show normal gastric mucosa comprising foveolar epithelium without any inflammation or necrosis. Micrographs of group (E) CN (control), (F) ANP 50 treated at 50 mg/kg, (G) ANP 300 treated at 300 mg/kg, and (H) ANP 2000 treated at 2,000 mg/kg display normally arranged and maintained polarity of myocytes arranged in muscle bundles. Sections of group (I) CN (control), (J) ANP 50 treated at 50 mg/kg (K) ANP 300 treated at 300 mg/kg, and (L) ANP 2000 treated at 2,000 mg/kg show normally arranged astrocytes, oligodendrocytes, axons, and interspersed capillaries in a fibrillary background. Micrographs of group (M) CN (control), (N) ANP 50 treated at 50 mg/kg, (O) ANP 300 treated at 300 mg/kg, and (P) ANP 2000 treated at 2,000 mg/kg show normal white and red pulp of the spleen with a profused lymphoid population.
Frontiers in Pharmacology frontiersin.org 13 TABLE 4 Effect of single oral administration of different doses of Artemisia absinthium extract-loaded NPs on the hematological parameters, liver profile, kidney profile, and body weight of female Wistar rats after 14 days of acute oral toxicity study.
lamina propria which, in turn, indicates that ANPs increased gut permeability in rats. Additionally, adverse clinical signs, including diarrhea and loss of appetite, were seen in group ANP 2000 , which was consistent with our finding of intestinal ulceration and inflammation, indicating disturbed intestinal function accompanied by diarrhea at a high dose of ANPs (Wang et al., 2019). Necropsy analysis of animals treated at 2,000 mg/kg also revealed the deposition of a small amount of ANPs in the lumen of the intestine. These findings can be correlated with an in vitro release study that reported a remarkable increment in LCST of the pH-and temperature-responsive, insulin-loaded NIPAAM and AA derivative-based hydrogels under the condition of neutral artificial intestinal fluid (pH 6.8), resulting in hydrogel swelling and fast release of insulin (Gao et al., 2013). Thus, we can anticipate that ANPs would also have experienced an increase in LCST (>37°C) at intestinal pH, eventually collapsing and releasing its content. However, our study cannot infer the amount of ANPs collapsing at intestinal pH and the amount of ANPs entering the systemic circulation and eventually getting eliminated from the body.
Several studies suggested that NPs entering systemic circulation are further distributed to different organs triggering further pathological changes (Chen et al., 2006). In this study, the liver of rats from all the groups showed dilatation of sinusoids and few central veins with interspersed congested blood vessels as a functional consequence of impaired blood flow. The kidney is the blood-filtering organ that receives a major portion of the systemic circulation; thus, direct exposure and accumulation of NPs can be responsible for renal tissue rupture or hemorrhage. In this study, the kidneys of animals treated at 300 mg/kg and 2,000 mg/kg showed focal areas of hemorrhage in the renal cortex without traces of ischemia, necrosis, or any other structural disintegration. The kidneys of animals treated at 50 mg/kg were also histopathologically normal with focal areas of inflammatory infiltrates in the cortex. Thus, we can postulate that these morphological and structural changes were associated with the fact that ANPs were able to pass through the intestinal epithelium and reached the lamina propria through which they are phagocytosed and transported to the kidney causing acute renal injury. These pathological changes due to a single oral dose persisted among animals treated at 50 mg/kg and 300 mg/kg ANPs even after 14 days of acute exposure advocate toward their capacity to cause moderate-to-mild renal toxicity. However, no pathological damage or toxicity of these ANPs was found in the stomach, heart, brain, or spleen. Therefore, the in vivo behavior, role of hydrodynamic size, nature of interaction with the intestinal tract, and fate in the GI tract require further investigation.

Conclusion
The findings of our study demonstrate that A. absinthium extract-loaded NIPAAM-VP-AA polymeric NPs (ANPs) have LD50 cut-off value equal to 500 mg/kg body weight and belong to 'category 4' of the GHS, with moderately toxic nature as high dose caused intestinal and renal injury in rats. Our preliminary study exhibits limitations being only concerned with the assessment of the LD50 cut-off value of ANPs, although providing a new insight into the understanding of immunostimulation by ANPs and emphasizing the need of investigating the hemocompatibility and chronic toxicity of ANPs for clinical application. However, an appropriate dose of these NPs presents a promising site-specific drug delivery system that could be employed against different medical disorders.

Data availability statement
The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding author.

Ethics statement
The animal study was reviewed and approved by the Institutional Animal Ethics Committee (IAEC) constituted through the Committee for the Purpose of Control and Supervision of Experiments on Animals (173/GO/Re/S/2000/CPCSEA) under the Ministry of Animal Welfare Division, Government of India, New Delhi.

Author contributions
SK was involved in data curation, formal analysis, investigation, methodology, validation, visualization, funding acquisition, writing-original draft, and writing-review and editing. MM was involved in methodology, project administration, and writing-review and editing. SS helped in investigation, methodology, and writing-review and editing. SW was involved in conceptualization, formal analysis, funding acquisition, investigation, methodology, project administration, resources, supervision, validation, visualization, and writing-review and editing. All authors contributed to the article and approved the submitted version.

Funding
The authors acknowledge the financial assistance by the Council of Scientific and Industrial Research (CSIR), India, by providing fellowship to SK and SS with award letter no. 09/591(0158)/2018-EMR-I and 09/591(0159)/2018-EMR-I, respectively.

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