Suppression of inflammation-induced lung cancer cells proliferation and metastasis by exiguaflavanone A and exiguaflavanone B from Sophora exigua root extract through NLRP3 inflammasome pathway inhibition

Objective: Non-small cell lung cancer (NSCLC) is recognized for its aggressive nature and propensity for high rates of metastasis. The NLRP3 inflammasome pathway plays a vital role in the progression of NSCLC. This study aimed to investigate the effects of S. exigua extract and its active compounds on NLRP3 regulation in NSCLC using an in vitro model. Methods: S. exigua was extracted using hexane, ethyl acetate and ethanol to obtain S. exigua hexane fraction (SE-Hex), S. exigua ethyl acetate fraction (SE-EA), and S. exigua ethanol fraction (SE-EtOH) respectively. The active compounds were identified using column chromatography and NMR analysis. A549 cells were primed with lipopolysaccharide (LPS) and adenosine triphosphate (ATP) for activated NLRP3 inflammasome. The anti-inflammatory properties were determined using ELISA assay. The anti-proliferation and anti-metastasis properties against LPS-ATP-induced A549 cells were determined by colony formation, cell cycle, wound healing, and trans-well migration and invasion assays. The inflammatory gene expressions and molecular mechanism were determined using RT-qPCR and Western blot analysis, respectively. Results: SE-EA exhibited the greatest anti-inflammation properties compared with other two fractions as evidenced by the significant inhibition of IL-1β, IL-18, and IL-6, cytokine productions from LPS-ATP-induced A549 cells in a dose-dependent manner (p < 0.05). The analysis of active compounds revealed exiguaflavanone A (EGF-A) and exiguaflavanone B (EGF-B) as the major compounds present in SE-EA. Then, SE-EA and its major compound were investigated for the anti-proliferation and anti-metastasis properties. It was found that SE-EA, EGF-A, and EGF-B could inhibit the proliferation of LPS-ATP-induced A549 cells through cell cycle arrest induction at the G0/G1 phase and reducing the expression of cell cycle regulator proteins. Furthermore, SE-EA and its major compounds dose-dependently suppressed migration and invasion of LPS-ATP-induced A549 cells. At the molecular level, SE-EA, EGF-A, and EGF-B significantly downregulated the mRNA expression of IL-1β, IL-18, IL-6, and NLRP3 in LPS-ATP-induced A549 cells. Regarding the mechanistic study, SE-EA, EGF-A, and EGF-B inhibited NLRP3 inflammasome activation through suppressing NLRP3, ASC, pro-caspase-1(p50 form), and cleaved-caspase-1(p20 form) expressions. Conclusion: Targeting NLRP3 inflammasome pathway holds promise as a therapeutic approach to counteract pro-tumorigenic inflammation and develop novel treatments for NSCLC.


Introduction
Lung cancer is a major global health concern, being the second most prevalent form of cancer and the primary contributor to cancer-related fatalities on a global scale (Huang et al., 2022).It includes different subcategories, wherein non-small cell lung cancer (NSCLC) makes up the majority, representing approximately 85% of all cases (Alduais et al., 2023).Within NSCLC, there are several subtypes such as adenocarcinoma, squamous cell carcinoma, and large cell carcinoma, among others.However, the existing therapies for NSCLC, which include surgery, chemotherapy, and radiotherapy, are inadequate in effectively reducing the elevated mortality rates associated with the disease (Wu and Lin, 2022).Despite advancements in early detection and treatment of NSCLC, the prognosis for patients with this condition remains unfavorable, as evidenced by a less than 20% 5-year overall survival rate (Zappa and Mousa, 2016).The recurrence of tumors and the spread of metastases play a significant role in the unfavorable outcomes and serve as the primary factors contributing to mortality in NSCLC cases (Ko et al., 2021).Hence, acquiring a thorough comprehension of the cellular and molecular mechanisms that trigger and advance NSCLC is of utmost significance.
In recent times, alongside surgery, chemotherapy, and radiotherapy, there has been increasing attention on adjuvant therapies that focus on targeting the tumor microenvironment.A growing body of evidence suggests that chronic inflammation plays a pivotal role in the progression of cancer (Gomes et al., 2014;Stares et al., 2022).While acute inflammation serves as a protective mechanism against infectious pathogens, chronic inflammation is linked to DNA damage and tissue dysfunction, encompassing genetic and epigenetic alterations that contribute to the initiation and advancement of various types of cancer (Lee et al., 2009).The inflammasome is an intracellular complex composed of multiple proteins that activates the inflammatory response in tissues when exposed to various stimuli.Over 20 inflammasomes have been identified, among which the NLRP3 inflammasome has been extensively studied and widely recognized for its crucial involvement in inflammation (Moossavi et al., 2018).The NLRP3 inflammasome comprises three main components: the NOD-like receptor NLRP3, the adaptor protein apoptosisassociated speck-like protein containing a caspase recruitment domain (ASC), and pro-caspase-1.The activation of the NLRP3 inflammasome involves a two-step process.In the first step, pathogen-associated molecular patterns (PAMPs) or dangerassociated molecular patterns (DAMPs) such as viruses, bacteria, or lipopolysaccharide (LPS) stimulate the expression of NLRP3, pro-IL-1β, and pro-IL-18.In the second step, a secondary stimulus like adenosine 5′-triphosphate (ATP), silica, or monosodium urate (MSU) triggers the activation of the NLRP3 inflammasome.This leads to the assembly of the NLRP3, ASC, and

Sophora exigua extracts
Total phenolic content (mg GAE/g extract) Total flavonoid content (mg CE/g extract) pro-caspase-1 components, resulting in the cleavage of pro-IL-1β and pro-IL-18 into their active forms, and the subsequent release of these pro-inflammatory cytokines (He et al., 2016).An increasing body of evidence indicates that the NLRP3 inflammasome plays a pivotal role in the development and progression of various types of cancer, including gastrointestinal cancer, skin cancer, breast cancer, hepatocellular carcinoma, and lung cancer (Huang et al., 2019;Gouravani et al., 2020;Lin et al., 2021;Xu et al., 2021).Activation of the NLRP3 inflammasome leads to the release of inflammatory cytokines including IL-1β and IL-18 to promote tumorigenesis (Gouravani et al., 2020).The activation of the inflammasome has also been implicated in promoting the migration and metastasis of various types of tumor cells (Dupaul-Chicoine et al., 2015;Yao et al., 2019).Given these findings, targeting the NLRP3 inflammasome holds potential as a therapeutic strategy for the treatment of NSCLC.Sophora exigua Craib, commonly referred to as "Phit sa nat" in Thai, is a plant belonging to the Fabaceae family and is found in different regions of Thailand.This Thai traditional medicinal plant, S. exigua, is known for its antipyretic and antiinflammatory properties (Krishna et al., 2012;Wang et al., 2016a).S. exigua is commonly incorporated into various multi-herb medicinal preparations, including the traditional Thai formula known as "Kheaw-Hom" remedy.This traditional remedy, Kheaw-Hom, is recognized and listed in the National List of Essential Medicines 2011 in Thailand.This remedy consists of eighteen Thai medicinal plants (Sukkasem, 2015).For an extended period, S. exigua has been utilized in folk medicine as a treatment for fever, skin ailments, measles, and chickenpox (Sukkasem et al., 2016).Several species within the Sophora genus have been studies for their pharmacological activities and potential therapeutic applications.Some of the most notable species include Sophora flavescens, Sophora japonica and Sophora alopecuroides (Krishna et al., 2012).These species have a long history of use in traditional medicine, particularly in East Asian countries (Aly et al., 2019;Abd-Alla et al., 2021).It is worth noting that specific medical research for Sophira exigua may be limited.S. exigua root extract exhibited antioxidant and antimalarial properties in plasmodium berghei-infected mice (Kaewdana et al., 2021).The root extract of S. exigua has demonstrated antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA), indicating its potential in combating this antibiotic-resistant bacterial strain (Tsuchiya et al., 1996;Fakhimi et al., 2006).In Folk medicine, it is used in the treatment of fever and inflammation (Sukkasem et al., 2016).However, the effect of S. exigua root extract and its chemical constituents on NLRP3 inflammasome pathway in NSCLC remains largely unknown.Therefore, the underlying mechanisms and potential therapeutic applications of S. exigua and its active compounds on the anti-inflammation-related cancer properties should be explored.
In this study, the in vitro anti-inflammation-related cancer properties of S. exigua root extract and its bioactive compounds on NSCLC cell lines were investigated.The A549 cells were primed with lipopolysaccharide (LPS) and adenosine triphosphate (ATP) for activated NLRP3 inflammasome and treatment with S. exigua root extract or its bioactive compounds.The study examined the  Ruangrungsi et al. (Ruangrungsi et al., 1992).

Position
Exiguaflavanone A in CDCl 3 (our results, 500 MHz) Exiguaflavanone A in acetone-d 6 (Ruangrungsi's results) δ H (multi., J (Hz), integral) δ H (multi., J (Hz), integral) anti-inflammatory, anti-proliferative, and anti-metastatic properties of S. exigua.The findings revealed that S. exigua root extract and its bioactive compounds were able to suppress the proliferation and metastasis of NSCLC cells by inhibiting the activation of the NLRP3 inflammasome.These results provide scientific evidence supporting the potential use of S. exigua root extract or its bioactive compounds as therapeutic strategies for NSCLC, specifically by targeting the NLRP3 inflammasome pathway.

Herb materials and preparation of Sophora exigua extracts
Sophora exigua was collected from natural source in 2022 from Chaiyaphum province, Thailand.Plant materials were identified and validated by Angkhana Inta, Chiang Mai University, Sukanda Chaiyong, Trinnakorn Katekulaphan, and Methee Rungrojsakul, Chandrakasem Rajabhat University, Bangkok, Thailand.The voucher specimen numbers of S. exigua (No.WP6605, WP6606, and WP7184) were certified by the herbarium at the Queen Sirikit Botanical Garden, Chiang Mai province, Thailand which was kept for future reference.The air-dried roots of S. exigua plants (100 g) were collected from 12 different sources and subjected to extraction using hexane (500 mL × 3 times), ethyl acetate (500 mL × 3 times), and ethanol (500 mL × 3 times).The resulting hexane, ethyl acetate, and ethanol extracts were filtered and concentrated under reduced pressure, yielding three crude fractional extracts.Ruangrungsi et al. (1992).

Column chromatography
In the experimental procedure, silica gel grade 60 was employed as the stationary phase, packed into a 3 cm × 60 cm column.The initial crude fractional extract, weighing 5.0 g, was carefully applied to the upper portion of the silica gel within the column.To achieve effective separation of compounds, we employed varying ratios of hexane and ethyl acetate as mobile phase components.This strategic adjustment of the hexane and ethyl acetate ratios, which enhances polarity, played a crucial role in facilitating the separation of compounds of interest.Upon elution, fractions were systematically collected in individual test tubes, with each fraction comprising 8 mL of the eluted material.For the analysis of compound separation and identification, thin layer chromatography (TLC) was employed.A small aliquot from each fraction was spotted onto a TLC plate.The TLC plate was then introduced into a chamber containing a hexane and ethyl acetate mixture in a 2:1 ratio.Concurrently, a reference standard, specifically the crude fractional extract's ethyl acetate fraction, was also subjected to TLC analysis alongside the fractions.Once the solvent front had progressed approximately 0.5 cm from the top of the TLC plate, the plate was removed from the chamber and subjected to molybdate staining.In order to identify and isolate the desired sub-fraction containing active compounds, fractions exhibiting similar TLC patterns were meticulously combined.The verification of purity for the consolidated fractions was carried out using TLC.Subsequently, the prominent compounds present in the consolidated fractions underwent further analysis to elucidate their chemical structures.This was achieved through the utilization of nuclear magnetic resonance (NMR) spectroscopy, specifically employing a Bruker instrument (Bruker, Fällanden, Switzerland).

Total phenolic content
The total phenolic content of the S. exigua extracts used in this study was determined using a Folin-Ciocalteu assay.The Folin-Ciocalteu assay was used as a gold standard protocol for determination of total phenolic content that was descripted in the previous studies (Box, 1983;Noreen et al., 2017;Aryal et al., 2019).In summary, 0.4 mL of each concentration of the S. exigua extracts was mixed with 0.3 mL of 10% Folin-Ciocalteau reagent and kept in the dark at room temperature for 3 min.Subsequently, 0.3 mL of carbonated sodium (Na 2 CO 3 ) was added to the mixture and was further incubated in the dark at room temperature for an additional 30 min.The absorbance of the resulting mixture was measured at 765 nm using a UV-visible spectrophotometer (UV-1800, SHIMADZU CO., LTD).The absorbance values were then compared to a standard curve of gallic acid (GA).The total phenolic content of the S. exigua extracts was expressed as milligrams of gallic acid equivalents per Gram of the herbal extracts (mg GAE/g extract).

Total flavonoid content
The total flavonoid content of the S. exigua extracts was determined using the aluminum chloride (AlCl 3 ) colorimetric assay with minor modifications from a previous study (Pękal and Pyrzynska, 2014).Catechin was used as the gold standard for comparison.In this assay, each concentration of the S. exigua extracts (250 µL) was mixed with 5% NaNO 2 (125 µL) and incubated for 5 min.Subsequently, 10% AlCl 3 (125 µL) was added to the mixture, followed by an additional 5 min of incubation.Afterward, 1.0 mL of NaOH was added, and the mixture was incubated for 15 min at room temperature.The absorbance of the resulting mixture was measured at 510 nm using a spectrophotometer and compared to a standard catechin.The total flavonoid content was expressed as milligrams of catechin equivalents (CE) per Gram of extract (mg CE/g extract).

Cell culture
The human lung adenocarcinoma cell line (A549) (CCL-185 ™ ) was obtained from American Type Culture Collection (ATCC) (Manassas, VA, United States).The cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) medium supplemented with 10% FBS, 50 IU/mL penicillin, and 50 μg/mL streptomycin.The cells were maintained in a humidified incubator at 37 °C with an atmosphere consisting of 95% air and 5% CO 2 .When the cells reached 70%-80% confluency, they were harvested and seeded for subsequent experiments.

The cell viability assay
The cytotoxicity of S. exigua extracts and its active compounds, exiguaflavanone A (EGF-A) and exiguaflavanone B (EGF-B), against A549 cells was determined by a sulforhodamine B (SRB) assay as was previously described (Vichai and Kirtikara, 2006;Sodde et al., 2015;Kabała-Dzik et al., 2017).In this study, we aimed to assess the cytotoxicity of S. exigua extracts and its active compounds over the course of our experiment, spanning 24 and 48 h.Thus, we made slight modifications to the protocols to tailor them to our specific experimental conditions.These modifications allow us to capture the dynamic effects of the test compounds within our desired timeframe, enhancing the precision of our investigation.Briefly, A549 cells (3 × 10 3 cells/well) were seeded in a 96-well plate and incubated at 37 °C, 5% CO 2 overnight.After that, the cells were treated with or without various concentrations of S. exigua extracts (0-500 μg/mL) and EGF-A (0-118 μM; MW = 424.5 g/mol) and EGF-B (0-114 μM; MW = 438.5 g/mol) for 24, and 48 h to observe the dose-response curve.Following incubation, the cells were treated with 10% (w/v) trichloroacetic acid (TCA) and incubated at 4 °C for 1 h.Subsequently, the medium was aspirated, and the cells were rinsed with gently running tap water.Next, 100 μL of a 0.054% (w/v) solution of sulforhodamine B (SRB) was added to each well, and the cells were incubated at room temperature for 30 min.After the incubation period, the SRB solution was removed, and the cells were washed four times with 1% (v/v) acetic acid.The cells were then allowed to dry at room temperature.To dissolve the dye, 150 μL of a 10 mM tris-based solution (pH 10.5) was added to each well, and the absorbance was measured at 510 nm using a microplate reader.The cell viability was calculated by comparing the absorbance of the treated cells to that of the control cells.

Determination of cytokine production
The levels of pro-inflammatory cytokines, specifically IL-1β, IL-18, and IL-6, in the cell culture supernatants were measured using an ELISA kit sourced from Biolegend (San Diego, CA, United States), following the manufacturer's protocol as previously outlined (Arjsri et al., 2022).A549 cells were seeded in a 6-well plate at a density of 2 × 10 5 cells per well.After overnight incubation, the cells were treated with various concentrations (0-7.5 μg/mL) of S. exigua extracts or its active compounds, exiguaflavanone A (EGF-A) and exiguaflavanone B (EGF-B) (0-4 μg/mL), for 4 h.Lipopolysaccharide (LPS) and adenosine triphosphate (ATP) could stimulate the inflammatory responds, one of them was NLRP3 inflammasome pathway (Wang et al., 2016b;Huang et al., 2017).Therefore, the cells were induced by LPS at a concentration of 1,000 ng/mL for 18 h, followed by the ATP at a concentration of 5 nM for an additional 6 h.The culture supernatant was collected for ELISA analysis to measure cytokine release.Calibration standards for IL-6, IL-1β, and IL-18 (ranging from 0 to 500 pg/mL) were utilized to establish a standard curve for the ELISA assay as an internal quality control during the quantitative ELISA.The amount of cytokine released into the supernatant was calculated and compared to the standard curves provided by the ELISA kit.

Colony formation assay
The inhibitory effect of S. exigua extract and its active compounds, exiguaflavanone A (EGF-A) and exiguaflavanone B (EGF-B), on A549 cells proliferation were determined using a colony formation assay, as per the established methodology outlined in the previous protocol (Franken et al., 2006).The A549 cells were seeded at 500 cells/ well in a 6-well plate and incubated for 24 h.The cells were pre-treated with 0-40 μg/mL of SE-EA and 0-15 μg/mL of EGF-A and EGF-B for 24 h.Then, the cells were induced by lipopolysaccharide (LPS) at concentration 1,000 ng/mL for 18 h following with adenosine triphosphate (ATP) 5 nM for further 7 days before harvest.The cells were fixed with 6% glutaraldehyde for 30 min and stained with Toluidine dye for 15 min.The dye was removed by water.The stained cells were visualized, and the images were captured using the iBright ™ CL-1500 imaging system (Thermo Fisher Scientific).The colony numbers were calculated using the "Analyze Particles."function in ImageJ 1.410 software (https://imagej.nih.gov/ij/).The parameters were set to include colonies of provide specific parameters used, e.g., size range, circularity, etc., ensuring accurate quantification.In each experiment, determinations were carried out in triplicate.

Cell cycle assay
To investigate the distribution of the cell cycle, we followed a previously established method (Crowley et al., 2016).A549 cells

FIGURE 5
The effect of SE-EA and its active compounds (EGF-A and EGF-B) on cell cycle distribution in LPS-ATP-induced A549 cells.The cells were treated with SE-EA (A, B) at a concentration range of 0-40 μg/mL or active compounds, EGF-A (C, D) or EGF-B (E, F), at a concentration range of 0-15 μg/mL (0-35.33 µM) and 0-15 μg/mL (0-34.20 µM), respectively, for 4 h.The cells were then induced by lipopolysaccharide (LPS) at a concentration 1,000 ng/ mL for 18 h, followed by adenosine triphosphate (ATP) at 5 nM for a further 6 h.After staining the cells with PI dye, cell cycle distribution was analyzed by flow cytometry (A, C, E) and the percentages of cells in the G0, S and G2/M phase were quantified (B, D, F).The data were obtained from three independent experiments and are presented as mean ± S.D. values.Statistical significance was determined with respect to the LPS-ATP-induced control group, and indicated as *p < 0.05, **p < 0.01 and ***p < 0.001.
were seeded at a density of 1 × 10 5 cells per well in a 6-well plate and cultured with 0.5% fetal bovine serum (FBS) in DMEM for 18 h.After the starvation period, the cells were treated with various doses of SE-EA or its active compounds, exiguaflavanone A (EGF-A), and exiguaflavanone B (EGF-B).The cells were incubated with SE-EA or EGF-A or EGF-B for 4 h.Then, the cells were induced by lipopolysaccharide (LPS) at concentration 1,000 ng/mL for 18 h following with adenosine triphosphate (ATP) 5 nM for further 6 h before harvest.Following the treatment, the cells were harvested and processed for cell cycle analysis.This typically involves trypsinizing the cells to detach them from the culture plate, fixing them in ice-cold 70% ethanol in −20 °C overnight.After washing the cell pellets with PBS, 12.5 µL of ribonuclease A (RNase A) was added to the pellets and incubated at 37 °C in a CO 2

FIGURE 6
The effects of SE-EA and its active compounds (EGF-A and EGF-B) on cell cycle regulator proteins (Cyclin D1, Cyclin E1, CDK-2, and CDK-4) expression in LPS-ATP-induced A549 cells.The cells were treated with SE-EA (A, B) at concentrations of 0-40 μg/mL or active compounds, EGF-A (C, D) or EGF-B (E, F), at a concentration range of 0-15 μg/mL (0-35.33 µM) and 0-15 μg/mL (0-34.20 µM), respectively, for 4 h.Following treatment, the cells were induced by lipopolysaccharide (LPS) at a concentration of 1,000 ng/mL for 18 h, followed by adenosine triphosphate (ATP) at a concentration of 5 nM for an additional 6 h before being harvested.The protein expression of Cyclin D1, Cyclin E1, CDK-2, and CDK-4 was determined by Western blot analysis, and the band density was measured using ImageJ 1.410 software.The LPS-ATP-induced A549 cells are presented as 100% of the control.Data are presented as mean ± S.D. values of three independent experiments.*p < 0.05, **p < 0.01 and ***p < 0.001 compared to the LPS-ATP-induced control group.
incubator for 15 min.Following the RNase A treatment, 200 µL of propidium iodide (PI) dye was added to the cell pellets and incubated at 37 °C in a CO2 incubator for 45 min.After the incubation period, the cells were centrifuged to remove the PI dye and the cell pellets were then re-suspended with 500 µL of PBS.The stained cells would then be subjected to flow cytometry analysis (Beckman Coulter Inc., Indiana, United States) to determine the distribution of cells in different phases of the cell cycle (G0/G1, S, and G2/M).

Wound-scratch assay
The wound-scratch assay was conducted to examine the migratory capacity of A549 cells as per the established protocol (Liang et al., 2007).Briefly, A549 cells were seeded at 2.5 × 10 5 cells in a 6-well plate and incubated for 24 h and then were cultured with 0.5% FBS in DMEM for overnight.A549 cells were cultured to 90%-100% confluence, a 200 µL pipette tip was used to generate a wound in the surface of the cells in a 6-well plate, and then the cells were treated with 0-7.5 μg/mL of SE-EA or (0-4 μg/mL) of its active compounds, exiguaflavanone A (EGF-A) and exiguaflavanone B (EGF-B) for 4 h.Then, the cells were induced by lipopolysaccharide (LPS) at concentration 1,000 ng/mL for 18 h following with (ATP) adenosine triphosphate 5 nM for further 6 h.Images of the same fields at the indicated time-points (0 and 24 h) were captured under a light microscope at a magnification of ×100.The images were captured under a phase-contrast microscope (Nikon Eclipse TS100, NIKON INSTRUMENT INC).The wound area was calculated using the " Wound healing size" plugin in ImageJ 1.410 software

FIGURE 7
The effects of SE-EA and its active compounds (EGF-A and EGF-B) on the migration of LPS-ATP-induced A549 cells.A scratch assay was used to assess the anti-migration effects of SE-EA (A) at concentrations of 0-7.5 μg/mL, EGF-A (B) at concentrations of 0-4 μg/mL (0-9.42 µM), and EGF-B (C) at concentrations of 0-4 μg/mL (0-9.12 µM).Photographs were taken at 0 and 24 h following the initial scratch assay.The trans-well migration assay was used to confirm the anti-metastasis properties of SE-EA (D), EGF-A (E), and EGF-B (F) against LPS-ATP-induced A549 cells.The migrated cells were photographed under phase-contrast microscopy at 0 and 24 h and quantified using ImageJ software.Data are presented as mean ± S.D. of three independent experiments.**p < 0.01 and ***p < 0.001 indicating statistical significance compared to the LPS-ATP-induced control group.(Suarez-Arnedo et al., 2020).The plugin parameters were adjusted to accurately delineate the wound area for each experimental condition.

Trans-well migration and invasion assay
To evaluate the impact of SE-EA (S. exigua extract), exiguaflavanone A (EGF-A), and exiguaflavanone B (EGF-B) on the invasion and migration of A549 cells, trans-well migration and invasion assays were performed following the previously outlined protocol (Pitchakarn et al., 2012).Polyvinylpyrrolidone-free polycarbonate filters with 8 μm pore size (BD Biosciences, Franklin Lakes, NJ, United States) were used.For the invasion assay, the filters were coated with 15 μg of Matrigel per filter, while for the migration assay, the filters were coated with 10 μg/ mL fibronectin.A549 cells were seeded into the upper chamber of the trans-well inserts at a density of 5 × 10 4 cells per well.The cells were cultured in 0.1% FBS DMEM containing different concentrations of SE-EA, EGF-A, or EGF-B (0-7.5 μg/mL for SE-EA and 0-4 μg/mL for EGF-A and EGF-B).The lower chamber of the trans-well was filled with 10% FBS DMEM, which acts as a chemoattractant.After 4 h of incubation, the cells were induced by lipopolysaccharide (LPS) at a concentration of 1,000 ng/mL for 18 h, followed by the addition of adenosine triphosphate (ATP) at a concentration of 5 nM for an additional 6 h.The invading or migrating cells on the lower surface of the trans-well filter were fixed with 95% ethanol for 5 min and then stained with 0.5% crystal violet in 20% methanol for 30 min.The stained cells were visualized under a phase-contrast microscope (Nikon Eclipse TS100, NIKON INSTRUMENT INC.), and images were captured.To quantify the extent of invasion or migration, the percentages of the areas occupied by cells were determined using the "Threshold" function in ImageJ 1.410 software (https://imagej.nih.gov/ij/).The threshold parameters were fine-tuned to distinguish cells and background, ensuring reliable assessment.

Determination of IL-6, IL-1β, IL-18 and NLRP3 gene expressions by RT-qPCR analysis
To quantify the gene expressions of IL-6, IL-1β, IL-18 and NLRP3 at 24 h after LPS + ATP induction, we adopted the

FIGURE 8
The effects of SE-EA and its active compounds (EGF-A and EGF-B) on the invasion of LPS-ATP-induced A549 cells.The anti-invasion effects of SE-EA (A) at concentrations ranging from 0-7.5 μg/mL, EGF-A (B) at concentrations of 0-4 μg/mL (0-9.42 µM), and EGF-B (C) at concentrations of 0-4 μg/ mL (0-9.12 µM) on LPS-ATP-induced A549 cells were determined using a trans-well invasion assay.The migrated cells were imaged under phasecontrast microscopy at 0 and 24 h and quantified using ImageJ software.The data from three independent experiments are presented as mean ± S.D. The statistical significance was indicated as **p < 0.01 and ***p < 0.001, compared to the LPS-ATP-induced control group.
RT-qPCR analysis method described by (Olcum et al., 2021;Semmarath et al., 2023).A549 cells were pre-treated with 0-7.5 μg/mL of S. exigua extracts or (0-4 μg/mL) of its active compounds, exiguaflavanone A (EGF-A) and exiguaflavanone B (EGF-B) for 4 h.Then, the cells were induced by lipopolysaccharide (LPS) at concentration 1,000 ng/mL for 18 h following with (ATP) adenosine triphosphate 5 nM for further 6 h.After incubation, total mRNA was isolated from the cells using TRI reagent ® , a commonly used reagent for RNA extraction.The concentration and purity of the isolated RNA were determined using NanoDrop ™ 2000/2000c   Spectrophotometers (Thermo Fisher Scientific, Waltham, MA, United States of America), which measure the absorbance of the RNA sample at specific wavelengths.The A260/A280 ratio (>1.8) indicates pure RNA without contamination.To obtain cDNA (complementary DNA), reverse transcription was performed using a Mastercycler ® nexus gradient machine (Eppendorf, GA, Germany).For quantitative real-time PCR (qRT-PCR), the cDNA samples were amplified and quantified using a qRT-PCR ABITM 7500 Fast and 7500 real-time PCR machine (Thermo Fisher Scientific, Waltham, MA, United States).This technique allows for the detection and quantification of specific target genes in the samples.According to the internal quality control during the qPCR, deionized water was use as a no-template control in every experiment.To assess the precision of our assays and ensure the reliability of the data, the duplicate runs have been performed for each sample and control.The gene expressions were analyzed using the software provided with the QuantStudio6 Flex real-time PCR system (Applied Biosystems).The 2 −ΔΔCT method with normalization to GAPDH and controls was used for calculation of results.All primer sequences used in this study were as follows: IL-6 forward, 59-ATG AAC TCC TTC ACA AGC-39, reverse, 59-GTT TTC TGC CAG TGC CTC TTT G-3'; IL-1β forward, 59-ATG ATG GCT TAT TAC AGT GGC AA-39, reverse, 59-GTC GGA GAT TCG TAG CTG GA -39; IL-18 forward, 59-AAA CTA TTT GTC GCA GGA ATA AAG AT -39 reverse, 59-GCT TGC CAA AGT AAT CTG ATT CC -39; NLRP3 forward, 59-AAG GGC CAT GGA CTA TTT CC -39 reverse, 59-GAC TCC ACC CGA TGA CAG TT -39and GAPDH forward, 59-TCA ACA GCG ACA CCC AC -39 reverse, 59-GGG TCT CTC TCT TCC TCT TGT G -39 (Humanizing Genomics Macrogen, Geumcheon-gu, Seoul, South Korea).

Western blot analysis
To determine the expression of cell cycle regulator proteins (CDK-2, CDK-4, cyclin D1 and cyclin E), The cells (5 × 10 5 cells per well) were plated in 6-well plate and cultured with 0.5% FBS in DMEM for 18 h before they were treated with 0-7.5 μg/mL of SE-EA or 0-4 μg/mL of its active compounds, exiguaflavanone A (EGF-A) and exiguaflavanone B (EGF-B) for 4 h.Then, the cells were induced inflammation by LPS at concentration 1,000 ng/mL for 18 h

FIGURE 9
Inhibitory effects of SE-EA extract and its active compounds (EGF-A and EGF-B) on the NLRP3, IL-1β and IL-18 gene expression in LPS-ATP-induced A549 cells.A549 cells were treated with SE-EA extract (A) at concentrations of 0-7.5 μg/mL or active compounds, EGF-A (B) at concentrations of 0-4 μg/mL (0-9.42 µM) or EGF-B (C), at concentrations of 0-4 μg/mL (0-9.12 µM) for 4 h.After treatment, the cells were induced by lipopolysaccharide (LPS) at a concentration of 1,000 ng/mL for 18 h, followed by adenosine triphosphate (ATP) at 5 nM for a further 6 h.The mRNA expressions of NLRP3, IL-6, IL-1β and IL-18 were determined using RT-qPCR.The LPS-ATP-induced A549 cells were used as a control group, and their expression levels were considered as 100%.The data are presented as mean ± S.D. values of three independent experiments.Statistical analysis was performed, *p < 0.05, **p < 0.01 and ***p < 0.001 were considered statistically significant compared to the LPS-ATP -induced control group.
followed by ATP adenosine triphosphate 5 nM for further 6 h before harvested.In order to determine the effects of SE-EA or its active compounds, exiguaflavanone A (EGF-A) and exiguaflavanone B (EGF-B) on NLRP3 inflammasome pathway, the A549 cells were pre-treated with 0-7.5 μg/mL of SE-EA or 0-4 μg/mL of its active compounds, exiguaflavanone A (EGF-A) and exiguaflavanone B (EGF-B) for 4 h.After inducing inflammation by lipopolysaccharide (LPS) and adenosine triphosphate (ATP) for 6 h, the cells were harvested, and the resulting cell pellets were lysed using RIPA buffer, adhering to the methodology outlined in the previous study (Chittasupho et al., 2023).RIPA buffer contains detergents and salts that help to solubilize and extract proteins from the cells.The protein concentration in the lysate was determined using the Bradford assay, which is a commonly used method for protein quantification.The Bradford assay relies on the binding of Coomassie Brilliant Blue dye to proteins, resulting in a color change that can be measured spectrophotometrically.For protein analysis, the whole-cell lysate was subjected to 10% SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis).To prevent non-specific binding, the membranes were blocked with 5% bovine serum albumin (BSA) in 0.5% TBS-Tween.After blocking, the membranes were washed with 0.5% TBS-Tween to remove excess BSA and unbound proteins.Following the cyclin D1, cyclin E1, CDK-2, CDK-4, NLRP3, ASC, or caspase-1 primary antibody incubation, the membranes were washed multiple times with 0.5% TBS-Tween to remove any unbound primary antibody.Subsequently, the membranes were incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody, either antimouse or anti-rabbit IgG, depending on the primary antibody used.Finally, the membranes were exposed to an imaging system such as the iBright ™ CL-1500 imaging system (Thermo Fisher Scientific) to capture the chemiluminescent signal.The resulting images were used for the analysis of band density levels using the "Measure" function in ImageJ 1.410 software (https://imagej.nih.gov/ij/).

Statistical analysis
The experiments were performed in triplicate independent experiments to ensure reproducibility.The data obtained from each experiment were presented as the mean ± standard deviation (mean ± SD), which provides an indication of the variability within the data set.Statistical analysis was performed using Prism version 8.0 software, which is a commonly used statistical analysis tool.The independent t-test and one-way ANOVA with Dunnett's test were used for data analysis in various experiments.The significance level for determining statistical significance was set at *p < 0.05, **p < 0.01, and ***p < 0.001.These values indicate the probability of obtaining the observed results by chance.A smaller p-value indicates a higher level of statistical significance.

Extraction of Sophora exigua and phytochemicals study
After subjecting S. exigua to solvent partition extraction, three fractions were obtained: S. exigua hexane extract (SE-Hex), S. exigua ethyl acetate extract (SE-EA), S. exigua ethanolic extract (SE-EtOH).Table 1 displays the total phenolic and total flavonoid contents of each fraction.The results indicate that SE-EA has the highest total phenolic content (152.12 ± 8.73 mg of GAE/g extract) and the total flavonoid content (112.75 ± 4.32 mg of CE/g extract), followed by SE-EtOH and SE-Hex respectively.Thus, the solvent partition extraction technique can increase the phenolic content in the extracts, as observed in SE-EA, this fraction can be used further to identify the bioactive phytochemical compounds present.Among the three fractional extracts tested, the ethyl acetate extract had the highest yield, with a value of 15.8 ± 0.05, followed by the ethanolic and hexane extracts with values of 4.53 ± 0.06, and 3.88% ± 0.09%, respectively.The ethyl acetate extract (5 g) was further purified using silica gel column chromatography, yielding two dominant compounds with yields of 3.94% and 5.96%, respectively.The compounds were identified as exiguaflavanone A and exiguaflavanone B through comparison of their 1 H-NMR spectra (Figure 1; Tables 2, 3) with previously published data (Ruangrungsi et al., 1992).Exiguaflavanone A was obtained as a brown gum-like material (purity degree is >90%), while exiguaflavanone B was obtained as a yellow viscous oil (purity degree is >90%).

Effects of S. exigua extracts and its active compounds on A549 cells viability
Prior to investigating the anti-inflammatory properties of S. exigua extracts and its bioactive compounds, the cytotoxic effects of SE-EA and its bioactive compounds on A549 cells were evaluated.The viability of A549 lung cells was measured using SRB assay after treatment with different concentrations of SE-Hex, SE-EA, and SE-EtOH, as well as exiguaflavanone A and exiguaflavanone B, for 24 and 48 h.The results revealed that SE-EA exhibited a cytotoxic effect on A549 cells, with an IC 50 of 64.00 ± 6.46 and 37.00 ± 1.53 μg/ mL after 24 and 48 h of incubation, respectively.On the other hand, SE-Hex had a higher IC 50 value of 111.67 ± 7.64 and 85.00 ± 5.00 μg/ mL after 24 and 48 h of incubation, respectively (Figure 2), indicating less cytotoxicity than SE-EA.SE-EtOH did not exhibit any cytotoxic effects on A549 cells at any concentration or time point tested.Exiguaflavanone A and exiguaflavanone B also showed cytotoxicity on A549 cells, with an IC 50 of 88.74 ± 11.14 and 68.42 ± 7.48 µM, respectively after 24 h of incubation, and with an IC 50 of 34.94 ± 6.05 and 30.03 ± 2.37 µM, respectively, after 48 h of incubation.Non-toxic concentrations of SE-Hex, SE-EtOH, SE-EA (0-7.5 μg/mL), and the active compounds exiguaflavanone A (0-9.42 µM or 0-4 μg/mL) and exiguaflavanone B (0-9.12 µM or 0-4 μg/mL) were selected for further experiments to investigate their anti-inflammatory properties on A549 cells.

Anti-inflammatory effects of S. exigua extracts and its active compounds on LPS-ATP-induced A549 cells
To assess the anti-inflammatory effects of S. exigua extracts (SE-Hex, SE-EtOH and SE-EA) and their bioactive compounds (exiguaflavanone A and exiguaflavanone B) on LPS-ATP-induced inflammation, we measured the release of pro-inflammatory cytokines  in the supernatant of A549 cells using ELISA.The levels of these cytokines were significantly increased in LPS-ATP-induced A549 cells compared to non-induced cells (p < 0.001), as shown in Figure 3. Treatment with SE-EA resulted in a dose dependent decrease in the release of IL-6, IL-1β, and IL-18 from LPS-ATP-induced A549 cells (p < 0.001).However, SE-Hex and SE-EtOH did not exhibit any inhibitory effects on cytokine releases (IL-6, IL-1β, and IL-18), as shown in Figure 3A.Moreover, the bioactive compounds exiguaflavanone A and exiguaflavanone B also demonstrated significant inhibition of IL-6, IL-1β, and IL-18 release from LPS-ATP-induced A549 cells in a dose-dependent manner (p < 0.001), as shown in Figures 3B, C.These results indicate that SE-EA and its bioactive compounds, exiguaflavanone A and exiguaflavanone B, possess anti-inflammatory properties against LPS-ATP inducted inflammation by reducing the release of IL-6, IL-1β, and IL-18 cytokines in A549 cell culture supernatant.

Effects of SE-EA and its active compounds (EGF-A and EGF-B) on LPS-ATP-induced A549 cells proliferation
Based on previous research, it is known that the activation of NLRP3 inflammasome can promote cancer progression by releasing inflammatory cytokines such as IL-1β.In this study, A 549 lung cancer cells were induced with inflammation through the NLRP3 inflammasome pathways using LPS bacterial inflammation-inducing agents in combination with adenosine triphosphate (ATP), and measured cell proliferation using colony formation assay.The result indicated that lung cancer cells induced by LPS and ATP showed a greater ability to proliferate than lung cancer cells that were not induced with inflammation in A549 cells, as shown in Figure 4. Next, the effectiveness of SE-EA extract and its active compounds, EGF-A and EGF-B, in inhibiting the growth of A549 lung cancer cells were investigated, it was found that the SE-EA extract (Figure 4A) as well as EGF-A (Figure 4B) and EGF-B (Figure 4C) had significant inhibitory effects on the proliferation of A549 lung cancer cells induced by LPS and ATP.To further understand the mechanism by which SE-EA extract and its active compounds, EGF-A and EGF-B, inhibit cell growth, we conducted a cell cycle analysis assay.As shown in Figure 5, the results showed that the SE-EA (Figures 5A, B) and its active compounds EGF-A (Figures 5C, D) and EGF-B (Figures 5E, F) were able to arrest the growth of A 549 lung cancer cells induced by LPS and ATP at the G1 phase of the cell cycle.To gain insight into the underlying mechanism of SE-EA and its bioactive compounds, Western blotting analysis was conducted to assess the expression of cell cycle regulator proteins.The results showed that SE-EA, EGF-A, and EGF-B markedly reduced the expression of cyclin D1, cyclin E1, CDK-2, and CDK-4 in LPS-ATP-induced A549 cells in a dose-dependent manner, as shown in Figure 6.

Effects of SE-EA and its active compounds (EGF-A and EGF-B) on LPS-ATP-induced A549 cells migration
Inflammatory response of lung cancer cells through the NLRP3 inflammasome pathway not only promotes cancer cell growth but also enhances cancer cell invasion and metastasis.Therefore, the effects of the SE-EA extract and its two active compounds, EGF-A and EGF-B, on the inhibition of lung cancer cell migration were investigated using would healing and trans-well assays in LPS-ATP-induced A549 lung cancer cells.The study found that LPS-ATP-induced A549 lung cancer cells had a higher migration capability than non-induced A549 cells, as shown in Figure 7.However, when treated with SE-EA extract, significant inhibition of cancer cell migration was observed in LPS-ATPinduced A549 lung cancer cells, as shown in Figures 7A, D.Moreover, the two active compounds, EGF-A and EGF-B, derived from SE-EA extract, also exhibited a significant inhibition of cancer cell migration in LPS-ATP-induced A549 lung cancer cells, as indicated in Figures 7B, C; Figures 7E, F.

Effects of SE-EA and its active compounds (EGF-A and EGF-B) on LPS-ATP-induced A549 cells invasion
The effect of SE-EA and its two major compounds, EGF-A and EGF-B, on the invasion of A549 lung cancer cells were investigated using trans-well assay.The results showed that A549 lung cancer cells stimulated by LPS and ATP exhibited a higher invasion capability than non-stimulated A549 lung cancer cells, as shown in Figure 8.However, treatment with SE-EA extract and its active compounds, EGF-A and EGF-B, significantly inhibited the invasion of A549 lung cancer cells via the trans-well assay.Therefore, it can be concluded that the inflammatory response triggered by LPS-ATP via the NLRP3 inflammasome pathway enhances the invasive capability of A549 lung cancer cells.SE-EA extract and its active compounds, EGF-A and EGF-B can effectively suppress the invasion of A549 lung cancer cells stimulated by LPS-ATP via the NLRP3 inflammasome pathway.
Effects of SE-EA and its active compounds (EGF-A and EGF-B) on inhibition of proinflammatory cytokines (IL-6, IL-1β, and IL-18) and NLRP3 gene expressions in LPS-ATP-induced A549 cells The study aimed to investigate the mechanism of action of SE-EA extract, specifically EGE-A and EGF-B, in inhibiting the expression of pro-inflammatory cytokines IL-6 and inflammasome related cytokines IL-1β and IL-18, as well as the NLRP3 protein at the mRNA level using RT-qPCR.The results demonstrated that treatment of lung cancer A549 cells with LPS and ATP induced the expression of IL-6 and inflammasome-related cytokines IL-1β and IL-18, as well as increased NLRP3 gene expression.Conversely, treatment with SE-EA extract (Figure 9A), EGF-A (Figure 9B), and EGF-B (Figure 9C) significantly inhibited the expression of IL-6, IL-1β, IL-18, and NLRP3 genes in A549 cells stimulated with LPS and ATP in a concentration-dependent manner (*p < 0.05, **p < 0.01 and ***p < 0.001), compared to the LPS and ATP only group.

Effects of SE-EA and its active compounds (EGF-A and EGF-B) on the NLRP3 inflammasome pathway in LPS-ATPinduced A549 cells
To induce inflammation, we treated A549 cells with LPS and ATP and compared these cells to a control group A549 cells that were not induced.It was found that, in A549 lung epithelial cells, the induction of inflammation using LPS and ATP resulted in elevated expression levels of NLRP3, ASC, pro-caspase-1 (p50), and cleavedcaspase-1 (p20) proteins when compared to non-induced control cells.Next, we performed Western blot analysis to evaluate the effects of SE-EA extract, EGF-A and EGF-B on the expression of these proteins in the LPS and ATP-induced A549 cells.Our results revealed that the SE-EA extract (Figures 10A, B), as well as EGF-A (Figures 10C, D) and EGF-B (Figures 10 E, F), significantly reduced the expression of NLRP3, ASC, pro-caspase-1 (p50), and cleavedcaspase-1 (p20) proteins in the LPS and ATP-induced A549 cells.

Discussion
Research on the anticancer potential of Sophora species, including Sophora flavescens and Sophora japonica, has yielded promising results in inhibiting the growth of cancer cells (Krishna et al., 2012;Chen et al., 2013;He et al., 2015).For example, Sophora flavescens, commonly used in traditional Chinese medicine, has shown potential as an anticancer agent against lung cancer cells.Extracts from Sophora flavescens have demonstrated anti-proliferative effects and induced apoptosis in A549 and NCI-H226 cell lines (Chen et al., 2019).Similarly, extracts from Sophora flavescens and Sophora japonica have been investigated for their potential anticancer effects in MCF-7 breast cancer (Cao and He, 2020).Studies have isolated certain compounds from Sophora species that exhibit promising anticancer effects against liver cancer cells.These compounds, including flavonoids and alkaloids, have demonstrated inhibitory effects on cell proliferation, migration, and invasion in HepG2 cell lines (Lin et al., 2023).Sophora japonica and Sophora subprostrata have been studied for their potential anticancer effects on colorectal cancer cells.Extracts from these plants have shown cytotoxic activity against HT29 colorectal cancer cells and induced apoptosis in these cells (Fang et al., 2018;Chen et al., 2021).However, information specifically related to Sophora exigua and its anticancer potential is scarce.Hence, it is essential to study the anticancer properties of S. exigua plant extract and its bioactive compounds on the A549 non-small cell lung cancer cell line.Furthermore, investigating the underlying mechanisms involved in cancer progression is of utmost importance.
Exiguaflavanones, specifically flavanones, which are a subclass of flavonoids, are known for their diverse chemical structures and various biological activities (Ruangrungsi et al., 1992;Boozari et al., 2019;Abd-Alla et al., 2021).Flavanones have been studied for their potential health benefits and medicinal properties, including antioxidant, anti-inflammatory, antimicrobial, and anticancer activities (Tsuchiya et al., 1996;Fakhimi et al., 2006;Kaewdana et al., 2021).In the root of Sophora exigua, a plant known for its medicinal properties, two important flavanone compounds called Exiguaflavanone A (EGF-A) and exiguaflavanone (EGF-B) can be found.These compounds are obtained from the ethyl acetate fraction of the plant extract (SE-EA) and have yields of 3.94% and 5.96%, respectively.In the context of the NLRP3 inflammasome pathway, which plays a crucial role in the inflammatory response of lung cancer cells, we have expanded upon the mechanisms discussed in the references.Specifically, we have detailed the role of the NLRP3 inflammasome pathway in promoting cancer cell growth, invasion, and metastasis.This is achieved partly through the activation of the NLRP3 inflammasome pathway and the subsequent release of the IL-1β cytokine, as highlighted in previous studies (Fan et al., 2014;Wang et al., 2016b).Therefore, this study aimed to investigate the potential of the SE-EA extract and its active compounds, EGF-A and EGF-B, in inhibiting lung cancer cell proliferation, migration, and invasion.To induce inflammation in A549 non-small cell lung cancer cells, a combination of LPS, a bacterial inflammation-inducing agent, and ATP was used.The findings of our investigation revealed that SE-EA extract and its active compounds, EGF-A and EGF-B, effectively inhibited LPSand ATP-induced proliferation of A549 cells.These compounds caused cell cycle arrest at the G1 phase and significantly downregulated the expression of cell cycle regulator proteins.Moreover, the SE-EA extract and its active compounds demonstrated a significant inhibitory effect on LPs-ATP-induced cell migration and invasion.
Inflammation is a complex process that involves the release of various cytokines and mediators.Notably, IL-6, Il-1β, and IL-18 are well-known pro-inflammatory cytokines that play a crucial role in the pathogenesis of many inflammatory diseases (Fenini et al., 2017).Previous studies have indicated that the activation of NLRP3 inflammasome can contribute to cancer progression by releasing inflammatory cytokines such as IL-1β and IL-18 (Vidal-Vanaclocha et al., 2000;Fabbi et al., 2015;Fenini et al., 2017).Our findings confirm the anti-inflammatory effects of SE-EA and its active compounds, EGF-A and EGF-B, by modulating the NLRP3 inflammasome pathway.These compounds effectively reduce cytokine release and downregulate key proteins involved in the NLRP3 inflammasome pathway, including NLRP3, ASC, procaspase-1 and cleaved-caspase-1.Furthermore, in our molecular investigations, we observed significant downregulation of mRNA expression of IL-1β, IL-18, and NLRP3 in LPS-ATP-induced A549 cells treated with SE-EA, EGF-A, and EGF-B, ultimately leading to a decrease in the release of inflammatory cytokines.The downregulation suggested that these compounds have the ability to suppress the transcriptional activity of these proinflammatory molecules.One potential mechanism through which this downregulation may occur is by inhibiting the activation of NF-kB.Several studies have reported that natural compounds can exert their anti-inflammatory effects by modulating NF-kB activity (Song et al., 2018;Semmarath et al., 2022;Lan et al., 2023).These compounds can interfere with the suppression of NF-kB nuclear translocation.By disrupting these steps, the compounds prevent the transactivation of NF-kB target genes involved in the inflammatory response.While the precise mechanism through which SE-EA, EGF-A, and EGF-B inhibit NF-kB activation and subsequently suppress transcriptional activity is not specified in this study, it is reasonable to propose NF-kB as a potential target.Further investigations, such as assessing NF-kB activation status would be necessary to confirm this hypothesis and provide more mechanistic insights into the transcriptional regulation.
Our study also contributes to the understanding of the role of inflammation in cancer progression.This observation aligns with previous research that has established a link between inflammation and cancer.For instance, the overexpression of NLRP3 inflammasome stimulated proliferation, migration, and invasion of esophageal squamous cell carcinoma (ESCC) in vitro.
As evidence by the knocking down using NLRP3-siRNAs could result in the attenuation of ESCC metastatic potential.Hence, NLRP3 could be a promising new candidate of cancer targeted therapy (Yu et al., 2020).In the context of non-small cell lung cancer, the activation of the NLRP3 inflammasome pathway in response to LPS and ATP enhanced the cell proliferation and invasive capability of A549 cells.Furthermore, the caspase-1 inhibitor, Z-YVAD-FMK, suppressed LPS-ATP-induced cell migration and invasion by abolishing Akt phosphorylation and inhibiting ERK1/2 and CREB phosphorylation (Wang et al., 2016b).Our findings coincide with these previous reports, highlighting the potential influence of LPS and ATP-induced NLRP3 inflammasome pathway, consequently impacting cancer cell behavior.Moreover, the previous reports emphasize the crucial role of NLRP3 inflammasome in regulating the proliferation, invasion, and migration of A549 cells.These studies underline the significance of chronic inflammation as a recognized risk factor for cancer development (Moossavi et al., 2018;Gouravani et al., 2020;Xu et al., 2021).Inflammasomes can contribute to tumor development by influencing host tumor immunity, promoting tumor cell proliferation and differentiation, and regulating the tumor microenvironment.Notably, the activation of the NLRP3 inflammasome in cancer-associated fibroblast (CAF) or CAF-derived IL-1β could facilitate lung cancer metastasis, as demonstrated by Moossavi et al., 2018;Gouravani et al., 2020.Nevertheless, it is essential to acknowledge the context-dependent and sometimes contrasting effects of the NLRP3 inflammasome and its product, IL-1β, on tumorigenesis depending on various cancer cell types.Evidence from documented studies and in vivo experiments suggests the activation of inflammasomes and related cytokines, such as IL-1β, in many human cancers.IL-1β plays a pivotal role in connecting innate and adaptive immune responses (Ghiringhelli et al., 2009), inducing the polarization of IFN-γ-secreting CD8 + T cells and triggering the formation of IL-17-producing γδT cells, which γδT cells have the ability to recognize tumor-associated antigens and perform anti-cancer activities (Ma et al., 2011).However, recent studies have identified IL-1β as a pro-cancer factor due to its immunosuppressive and chemo-resistant properties (Voronov et al., 2003).In the context of our study, we align with these recent findings, as we have observed that NLRP3 activation contributes to the progression of cancer cells in A549 lung cancer cells.Given the complex role of NLRP 3 inflammasome in the initiation and progression of neoplasia, the NLRP3 inflammasome and its associated pathways represent promising therapeutic targets for the prevention and treatment of lung cancer.
Our findings suggest that SE-EA extract and its active compounds, EGF-A and EGF-B, possess potential anti-cancer properties as they inhibit the invasion of A549 lung cancer cells stimulated by LPS and ATP via the NLRP3 inflammasome pathway.This discovery is significant since invasion is a crucial feature of cancer progression and inhibiting this process could potentially slow down or even halt cancer growth.However, before considering the clinical application of SE-EA extract and its active compounds, EGF-A and EGF-B, further studies are necessary to elucidate the underlying mechanisms of their metabolic pathways and to evaluate potential side effects in animal models.Understanding the precise mechanisms by which these compounds exert their anti-inflammatory and anti-cancer effects is essential for their therapeutic potential.Additionally, a comprehensive assessment of any possible adverse effects is crucial to ensure the safety and efficacy of these compounds.Animal models provide a valuable platform for investigating the pharmacokinetics, metabolic pathways, and toxicity profiles of SE-EA extract and its active compounds, thus aiding in determining their suitability for clinical use.
In conclusion, this study investigated the effects of Sophora exigua extract and its active compounds on the NLRP3 inflammasome pathway in non-small cell lung cancer (NSCLC).The results demonstrated that the extract, particularly the ethyl acetate fraction (SE-EA), exhibited significant antiinflammatory properties and inhibited the production of proinflammatory cytokines in NSCLC cells.The active compounds, exiguaflavanone (EGF-A) and exiguaflavanone (EGF-B), were found to contribute to these effects.Moreover, SE-EA, EGF-A, and EGF-B showed anti-proliferative and anti-metastatic properties by affecting cell cycle progression, migration, and invasion in NSCLC cells.These findings suggest that targeting the NLRP3 inflammasome pathway could be a promising therapeutic approach for NSCLC treatment, providing insights into the development of novel anticancer therapeutics.
FIGURE 1 1 H NMR spectrums of purified compound spot (A) No. 1 and (B) No. 2 which were identified to be as exiguaflavanone A and exiguaflavanone B, respectively.

FIGURE 3
FIGURE 3Inhibitory effects of S. exigua extracts and its active compounds (EGF-A and EGF-B) on the pro-inflammatory cytokine release in LPS-ATP-induced A549 cells.A549 cells were treated with S. exigua extracts (A), SE-Hex, SE-EtOH, and SE-EA at a concentration of 0-7.5 μg/mL or active compounds, EGF-A (B) at a concentration of 0-4 μg/mL (0-9.42 µM) or EGF-B (C) at a concentration of 0-4 μg/mL (0-9.12 µM), for 4 h.Then, the cells were induced by lipopolysaccharide (LPS) at concentration 1,000 ng/mL for 18 h following with (ATP) adenosine triphosphate 5 nM for further 6 h.The IL-6, IL-1β and IL-18 releases into the culture supernatant were examined by ELISA.The LPS-ATP-induced A549 as 100%.Data are presented as mean ± S.D. values of three independent experiments, *p < 0.05, **p < 0.01 and ***p < 0.001 compared to the LPS-ATP-induced control group.

FIGURE 10
FIGURE 10Inhibitory effects of S. exigua extracts and its active compounds (EGF-A and EGF-B) on the NLRP3 inflammasome pathway in LPS-ATP-induced A549 cells.A549 cells were treated with SE-EA (A) at concentrations ranging from 0-7.5 μg/mL or active compounds, EGF-A (C) at concentrations of 0-4 μg/mL (0-9.42 µM) or EGF-B (E), at concentrations of 0-4 μg/mL (0-9.12 µM) for 4 h.The cells were induced by lipopolysaccharide (LPS) at a concentration of 1,000 ng/mL for 18 h, followed by adenosine triphosphate (ATP) at a concentration of 5 nM for a further 6 h.The inhibitory effects of SE-EA (A, B), EGF-A (C, D), and EGF-B (E, F) on the expression of NLRP3, ASC, and pro-caspase-1 and cleaved-caspase-1 proteins in A549 cells were measured using Western blot and band density measurements.The results were presented as a percentage of the LPS-ATP-induced A549 cells, where the control group was set to 100%.The data are presented as mean ± S.D. values of three independent experiments, with *p < 0.05, **p < 0.01 and ***p < 0.001 indicating statistical significance compared to the LPS-ATP-induced control group.

TABLE 1
Phytochemical study of S. exigua extracts.

TABLE 2 1
H-NMR spectral data of Exiguaflavanone A in comparison to those previously reported by

TABLE 3 1
H-NMR spectral data of Exiguaflavanone B in comparison to those previously reported by