Immunological role and prognostic value of somatostatin receptor family members in colon adenocarcinoma

Colon adenocarcinoma (COAD) is among the most prevalent cancers worldwide, ranking as the third most prevalent malignancy in incidence and mortality. The somatostatin receptor (SSTR) family comprises G-protein-coupled receptors (GPCRs), which couple to inhibitory G proteins (Gi and Go) upon binding to somatostatin (SST) analogs. GPCRs are involved in hormone release, neurotransmission, cell growth inhibition, and cancer suppression. However, their roles in COAD remain unclear. This study used bioinformatics to investigate the expression, prognosis, gene alterations, functional enrichment, and immunoregulatory effects of the SSTR family members in COAD. SSTR1-4 are differentially downregulated in COAD, and low SSTR2 expression indicates poor survival. Biological processes and gene expression enrichment of the SSTR family in COAD were further analyzed using the Kyoto Encyclopedia of Genes and Genomes and Gene Ontology. A strong correlation was observed between SSTR expression and immune cell infiltration. We also quantified SSTR2 expression in 25 COAD samples and adjacent normal tissues using quantitative real-time polymerase chain reaction. We analyzed its correlation with the dendritic cell–integrin subunit alpha X marker gene. The biomarker exploration of the solid tumors portal was used to confirm the correlation between SSTR2 with immunomodulators and immunotherapy responses. Our results identify SSTR2 as a promising target for COAD immunotherapy. Our findings provide new insights into the biological functions of the SSTR family and their implications for the prognosis of COAD.


Introduction
Colorectal cancer (CRC) is a common malignancy worldwide, ranking third among all malignancies in incidence and mortality (Shaukat and Levin, 2022).Colonic adenocarcinoma (COAD) is the most common form of CRC (Mutch, 2007).COAD progresses through several stages, from normal mucosa to adenoma, and finally to cancer (Riihimäki et al., 2016;Arnold et al., 2017).The usual presentation of COAD during medical treatment includes changes in bowel movements; in addition to the onset of rectal bleeding, irondeficiency anemia, abdominal pain, weight loss, and loss of appetite (Thanikachalam and Khan, 2019); there are no typical or specific clinical signs.However, comprehensive testing is lacking in most areas.Consequently, patients with COAD are commonly diagnosed with advanced cancer, making treatment more challenging and affecting their prognosis (Raza et al., 2022).Therefore, the identification of new biomarkers and therapeutic targets to improve the survival of patients with COAD is urgently needed.
Somatostatin (SST) is an inhibitory peptide hormone produced by neuroendocrine, inflammatory, and immune cells found in the central nervous system (CNS) and in several peripheral tissues in response to cytokines, growth factors, thyroid and steroid hormones, neurotransmitters, neuropeptides, nutrients, and ions (Patel, 1999;Wu et al., 2020).SST binds to certain cell surface receptors to suppress exocrine and endocrine secretions as well as tumor cell growth (Priyadarshini et al., 2022).Five different subtypes of SST receptors (SSTRs) (SSTR1-5) have been identified.SSTR is a member of the family of G protein-coupled receptors.The five isoforms are coupled with the Gi protein, which can affect the concentration of intracellular cyclic AMP (cAMP) by regulating the activity of adenylate cyclase and transmitting exogenous signals to cells (Bo et al., 2022;Liguz-Lecznar et al., 2022).SSTRs, which help release hormones, transmit nerve signals, arrest cell growth, and inhibit cancer, are found in abundance in the CNS and associated malignant cells as well as within the peripheral organs, pancreas, and gut (Rorsman and Huising, 2018;Harda et al., 2020).Neuroendocrine tumors (NETs) are a diverse category of tumors that can arise in the digestive tract, lungs, and pituitary among other organs (Klöppel, 2017).The expressions of SSTRs, which act as therapeutic targets for SST analogs, which can slow tumor growth and suppress hormone overproduction, are one inherent characteristic of NETs.Moreover, the degree of SSTR expression in several NETs has predictive significance for treatment response (Rogoza et al., 2022).Moreover, researchers found that the internalization capacity of SSTR and the development of radiolabeled somatostatin analogs have improved cancer diagnosis and treatment (Fani et al., 2017;Delpassand et al., 2022).
However, limited research has examined the expression, prognosis, and immune characteristics of the SSTR family members in COAD.Using research databases and bioinformatic methods, we examined SSTR family gene expressions and their link to clinical features in COAD.Our findings offer new knowledge about the prognosis and biological roles of SSTRs in COAD.

Tumor immune estimation resource database
The Tumor Immune Estimation Resource version 2.0 (TIMER2.0)database (https://cistrome.shinyapps.io/timer/) has three main components: vaccination, exploration, and assessment.TIMER2.0 can analyze relationships between genes and infiltrating cells, compare genetic expression in tumors and normal tissues across different malignant tumor types, and provide easy-to-use interactive visualizations to help explore the data (Li et al., 2020).Using the TIMER2.0database, we analyzed SSTR family expression in 41 normal and 457 COAD samples.Additionally, TIMER2.0 was employed to examine associations between the mRNA expression of SSTR family members and cells of the COAD immune infiltrate, including CD4 + and CD8 + T cells, neutrophils, macrophages, dendritic cells (DCs), and B cells.The R-value of the correlation was determined by Spearman's algorithm with adjustment for tumor purity.Values of p < 0.05 were considered significant.

Human protein atlas
Protein expression information for different cancer types based on immunohistochemical (IHC) analysis is available on the Human Protein Atlas (HPA) portal (http://www.proteinatlas.org).It is a critical resource for many biomedical research projects (Pontén et al., 2011).In this investigation, we conducted IHC to analyze the SSTR family members' protein expressions in normal and COAD tissues.

University of Alabama at birmingham cancer data analysis portal
University of Alabama at Birmingham Cancer Data Analysis Portal (UALCAN) (https://ualcan.path.uab.edu)makes it simple to perform Kaplan-Meier survival analyses depending on tumor subgroups, promoter DNA methylation status, and pre-calculated gene/protein expression (Chandrashekar et al., 2017).A stratified analysis was performed based on the individual patient's cancer stage and nodal status.Student's t-test was used, and values of p < 0. 05 were considered to be statistically significant.

CBioPortal
As a tool for interactively exploring multi-dimensional cancer genomic data sets, cBio Cancer Genomics Portal (http://cbioportal.org) is freely available (Cerami et al., 2012).We used cBioPortal to retrieve a dataset of 594 patients with COAD and conducted coexpression and gene alteration analyses of the SSTR family members.

STRING database
Known and predicted protein-protein association data of a large number of species are present in the STRING database (https://cn.string-db.org/).The database includes the physical interactions, functional links, and confidence levels that indicate their reliability.The STRING database was used to evaluate the SSTR gene correlations.

Cytoscape database
High-throughput expression data, other molecular states, and biomolecule interaction networks may be combined using the Cytoscape (http://cbioportal.org)open-source software project (Otasek et al., 2019).A total of 178 commonly mutated SSTR family genes were functionally integrated using Cytoscape after being screened from the cBioPortal database.Node size represents the degree values between these interacting proteins.Larger circles indicate a higher degree of interaction.

Metascape database
By leveraging more than 40 independent knowledge sources in one integrated platform, Metascape (https://metascape.org)integrates membership search, gene annotation, interactome analysis, and functional enrichment factors (Zhou et al., 2019).The SSTR family-related Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was conducted using Metascape.

PrognoScan
The correlation between SSTR expression and survival in COAD was analyzed using the PrognoScan database (http://www.abren.net/PrognoScan/) (Mizuno et al., 2009).PrognoScan offers a huge collection of freely accessible, clinically annotated cancer microarray datasets that may be applied to assess the biological links between gene expression and patient prognosis.Values of p < 0. 05 were considered statistically significant.

Biomarker exploration of solid tumors
Validation was conducted using the Biomarker Exploration of Solid Tumors (BEST) portal (https://rookieutopia.com/app_direct/BEST/).BEST was used to analyze the association between the SSTR family and immunotherapy response and prognosis in COAD.

Tissue samples
Twenty-five pairs of matched adjacent normal tissue samples and paraffin-embedded archival colon cancer specimens were obtained from Xiangya Hospital (Changsha, P. R. China).None of the patients had received any form of therapy, such as chemotherapy, radiotherapy, or immunotherapy, before the resection.The Xiangya Hospital of Central South University's research ethics committee approved the collection of clinical colon cancer specimens.

Isolation of RNA from formalin-fixed and paraffin-embedded samples
After the deparaffinization of formalin-fixed paraffin-embedded (FFPE) colon cancer or normal samples with xylene, total RNA was extracted using total RNA AmoyDx ® FFPE RNA Extraction Kit (Cat.#8.02.0019;AmoyDx, Xiamen, P. R. China).

Quantitative real-time polymerase chain reaction
As discussed previously, quantitative real-time polymerase chain reaction (qRT-PCR) was performed following the RNA extraction and amplification (Ou et al., 2020;He et al., 2021).Table 1 displays the qRT-PCR primer sequences.

Statistical analysis
The statistics for the survival analysis were obtained using the log-rank test, and the associations of the SSTR family with immune infiltration and markers of immune cell type were evaluated using Spearman's correlation.Student's t-test was conducted to contrast data from two independent samples.Values of p < 0.05 were considered statistically significant.

Aberrant expression of SSTR family members in COAD
To investigate the changes in SSTR expression levels in various malignant tissues compared to normal tissues, we employed the TIMER2.0database to assess the SSTR transcript levels.In COAD tissues, SSTR1-4 mRNA expression levels were remarkably downregulated.However, SSTR5 expression was upregulated (Figure 1A).Easy access to pre-calculated gene and protein expression based on tumor subgroups was provided by UALCAN (https://ualcan.path.uab.edu).This was applied to investigate the SSTR family gene mRNA expression.
Our findings revealed that all SSTRs except SSTR5 were significantly downregulated in COAD versus normal controls (p < 0.05) (Figure 1B).
To verify these results, we examined the SSTR family immunohistochemistry (IHC) results from the Human Protein Atlas database.SSTR1-4 protein levels were lower in COAD versus normal tissues (Figures 2A-D).These outcomes agreed with our earlier mRNA expression research findings.

Correlation of SSTR family expression with clinicopathological features of COAD
First, we investigated whether COAD staging and lymph node metastases were correlated with SSTR family member expression levels.In tumors with lymph node metastases at the N0-N2 stage, the mRNA expression levels of the four SSTR family genes (all but SSTR5) were lower (Figure 3A).Furthermore, compared to normal tissue, tumor stage 1-4 subgroups exhibited lower SSTR1-4 mRNA expression levels.A correlation occurred between SSTR1-2 expression and the different COAD stages (Figure 3B).These results suggested that SSTRs (SSTR2 in particular) contribute to COAD development.
Next, we examined the association between the clinicopathological characteristics of COAD and SSTR family gene expressions in The Cancer Genome Atlas Program database.Statistical analysis of the clinicopathological characteristics of 237 patients with COAD showed a correlation between SSTR1 expression and sex.SSTR4 expression was linked to age, N stage, and clinical stage, while SSTR1/2 expressions were substantially linked to lymphatic invasion (p < 0.05; Table 2).

Genetic alteration and functional analysis of SSTR family in patients with COAD
DNA methylation of COAD genes is a potential epigenetic biomarker for the early detection of COAD.The UALCAN database was used to determine the methylation levels of the SSTR genes in patients with COAD.In contrast to normal tissues, COAD samples had considerably lower levels of SSTR1/ 5 DNA methylation but remarkably higher SSTR2/4 methylation levels.For SSTR3, there were no remarkable variations between the normal and malignant tissues (Figure 5A).We subsequently used the cBioPortal dataset to investigate genetic alterations in each SSTR family member.All five SSTR family members were altered in patients with COAD, with alteration rates of 6%, 4%, 3%, 10%, and 5%, respectively (Figure 5B).The most prevalent SSTR family abnormalities in patients with COAD were mRNA alterations and mutations (Figure 5C).
Next, we used the Cytoscape v.3.9.0 database to identify coexpressed genes with a cutoff point of |log2 fold-change| ≥ 0.7 and p < 0.05.The co-expression network of key genes linked to the SSTR family was generated using the cBioPortal database (Figure 6A; Supplementary Table S1).The biological functions of the SSTR members and their co-expressed genes were assessed via GO annotation and KEGG pathway analyses using the Metascape database.For the co-expressed genes, KEGG pathway analysis was performed for cell adhesion molecules, the cAMP signaling pathway, and Staphylococcus aureus infection (Figure 6B).The GO findings illustrated that the co-expressed genes were primarily correlated with the pattern specification process, cell-cell signaling mediated by  Bold font indicates significant difference.
Frontiers in Pharmacology frontiersin.orga cell surface receptor pathway, and signaling receptor regulatory activity (Figure 6C).These genes were primarily involved in pattern specification, epithelial morphogenesis, and MARK cascade regulation (Figure 6D).A molecular function analysis revealed the primary involvement of these genes in signaling receptor regulatory activity, DNA-binding transcription activator activity, and ligand-gated monoatomic ion channel activity (Figure 6E).According to the analysis of cellular components, these genes were often linked to the extracellular matrix and the apical region of the cell (Figure 6F).
We analyzed the marker types of DCs, CD8 + T cells, neutrophils, and tumor-associated macrophages in COAD using the TIMER2.0database to further investigate the relationship between SSTR family expression and different immune cells (Table 3).We observed a correlation between SSTR1 and CD8 + T cells.We also observed a strong correlation between SSTR2 and CD8 + T cells, B cells, T cells, tumor-associated macrophages (TAMs), M2 macrophages, neutrophils, DCs, T helper type 1 (Th1) cells, Tfh cells, regulatory T cells (Tregs), exhausted T cells, and monocytes.A strong association occurred between CD8 + T cells, B cells,

FIGURE 6
SSTRs and SSTR-associated molecules co-expressed in COAD and their predicted functions and signaling pathways.(A) The cBioPortal database was used to identify the 178 SSTR-associated co-expressed molecules that are most frequently altered in COAD.SSTR family members and their associated co-expressed genes were used to generate the PPI network using the Cytoscape database.(B-F) Functional enrichment analysis was used to analyze the biological functions of the SSTR family members and their co-expressed genes.COAD, colon adenocarcinoma; PPI, protein-protein interaction; SSTRs, somatostatin receptors.
T cells, TAMs, M1 and M2 macrophages, neutrophils, DCs, natural killer (NK) cells, Th1, Th2, Tfh, Th17, Tregs, T-exhausted cells, and monocytes.SSTR4 expression was moderately correlated with B cells.A moderately strong correlation was observed between SSTR5 and M1 macrophage markers in patients with COAD.Furthermore, these results indicate that SSTR family members are likely to contribute to the immune infiltration of COAD.

Analysis and experimental verification of the potential value of SSTR2 expression in CRC immunotherapy
We performed preliminary experiments to verify the differential expression of SSTR2 in COAD and its correlation with immune cell-associated molecules to further investigate the role of SSTR2 in COAD.To characterize SSTR2 expression in COAD tissues, qRT-  PCR analyses revealed that the relative levels of SSTR2 expression in 25 COAD tissue samples were significantly lower than those in the matched adjacent non-tumor tissue samples (p = 0.0087; Figure 8A).We also demonstrated an association between SSTR and integrin subunit alpha X (ITGAX), a marker gene of DC.The qRT-PCR analysis showed that the relative expression levels of ITGAX were significantly lower in the 25 COAD samples than in the matched para-cancerous normal samples (p = 0.0086; Figure 8B).Finally, we found a positive correlation (r = 0.4248; p = 0.0343; Figure 8C) between SSTR2 and ITGAX expressions in the 25 COAD samples.
Based on the Gene Set Enrichment Analysis findings, SSTR2 was primarily positively enriched using the BEST tool of the following data.These genes are responsible for the regulation of antigen processing and presentation, inflammasomal complex assembly, regulation of cytotoxicity, and antigen processing and presentation of DCs (Figure 8D); cytokine receptor interaction; an intestinal immune network for IGA production; the chemokine signaling pathway; NK cell-mediated cytotoxicity; the Jak stat signaling pathway; the T cell receptor signaling pathway; and the Toll-like receptor signaling pathway in the KEGG analysis (Figure 8E).These results are consistent with the involvement of SSTR2 in functional immune networks in COAD.Furthermore, we analyzed the correlation between SSTR2 and immunomodulators among 10 datasets using BEST analysis, including antigen presentations, immune inhibitors, immunostimulators, chemokines, and chemokine receptors, to better understand the impact of SSTR2 on immunological responses.A substantial positive correlation between SSTR2 expression and the chemokine receptor CX3CR1; immunostimulators TNFRSFI3C and KLRKI; chemokines CCL16 and CCL1; and immune inhibitors IL10, BTLA, and KIR2DL1 are shown in Figure 9A.To further test the correlation between SSTR2 and immunotherapy, we examined whether aberrant SSTR2 expression influenced the response to immunotherapy in CRC.As shown in Figure 9B, a positive correlation was observed between the mRNA expression levels of SSTR2 and those of PDCD1 [programmed cell death protein 1 (PD-1), CD274 (programmed cell death protein ligand 1 (PD-L1)] (Huang et al., 2017), and cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) in The Cancer Genome Atlas Program dataset.SSTR2 expression was upregulated in chimeric antigen receptor T cell (CAR-T) responders in the Lauss cohort and anti-PD-1/PD-L1 responders in the Cho cohort (Figure 9C).The areas under the receiver operating characteristic curves for the Lauss and Cho cohorts were 0.933 and 0.782, respectively.This indicates that SSTR2 could discriminate between CAR-T and anti-PD-1/PD-L1 responders and nonresponders (Figure 9D).High SSTR2 expression correlated with better OS in CRC patients receiving CAR-T cells in the Lauss cohort (p < 0.05; Figure 9E).

Discussion
SSTRs have great potential for the diagnosis and treatment of cancer, as extensive research has illustrated the aberrant expressions of SSTR family members in several malignancies and their involvement in cancer cell proliferation and development (Rogoza et al., 2022;Aboagye et al., 2023).In vitro and in vivo studies suggest that SSTR1, SSTR2, and SSTR5 suppress the growth of pancreatic cancer (Li et al., 2005).High SSTR1 expression can silence and inhibit the proliferative rate of CRC stem cells by reducing the proliferation of acetaldehyde dehydrogenase-positive cells (Modarai et al., 2016).One preliminary study (Sun et al., 2021) confirmed that SSTR1 is a target gene affecting renal cell carcinoma (RCC) metastasis and the associated immune response and could be a prognostic biological marker of and viable therapeutic target for RCC.Another study (Zhou et al., 2009) found that the antiproliferative effects of SSTR2 were both cytostatic (growth suppression) and cytotoxic (apoptosis) by affecting the cellular apoptotic level, MAPK, and angiogenic signaling molecules in SSTR2-positive and -negative cancers.In the field of drug targeting, the delivery of nanoparticles through receptor-mediated cell interactions has received considerable attention.SSTRs are promising targets for various nanoparticles facilitated by modifying nanoparticles with specific ligands or coatings for better binding (Abdellatif et al., 2018).However, the function of SSTRs in COAD has not yet been thoroughly investigated.The five different perspectives of interest are mRNA and protein expression levels, clinical characteristics and disease prognosis, genetic mutations, pathway analysis, and immune infiltration.We extensively explained each SSTR family member's biological impacts on COAD.Compared to nontumor cells, we discovered that SSTR1-4 mRNA and protein levels were downregulated in COAD cells.Another novel finding was that the SSTR family is strongly associated with individual clinicopathological stages and nodal involvement in COAD.These data suggest that SSTR might be correlated with COAD progression and that SSTR family members are potential diagnostic markers for COAD.Additionally, the SSTR gene family is frequently altered in COAD.Changes in mRNA expression are among the prevalent mutations.These findings strongly indicate that the differential expression of SSTR family members may be essential for COAD.
As a receptor of the G protein-coupled signal transduction pathway, SSTR can prevent proto-oncogene activation, inhibit  cell proliferation, and inactivate tyrosine kinases through the MAPK pathway, thus preventing cellular proliferation (Chen et al., 2022;Sáez-Martínez et al., 2022).Next, we investigated the molecular and biological functions of SSTR family members.KEGG pathway analysis of SSTRs and their co-expressed genes revealed that cell adhesion molecules and the cAMP signaling pathway were significantly enriched.According to the GO pathway analysis, the cell surface receptor signaling pathway involved in cell-cell signaling and signaling receptor regulator activity was especially associated with SSTRs.The combination of SSTR2 or other subtypes with SST may inhibit DNA synthesis and play an antitumor role through the cAMP, MAPK, and other information pathways.This provides a fundamental principle for the development of multi-receptor SST analogs and combined therapy with signal-targeting agents such as mammalian (or mechanistic) target inhibitors of rapamycin (Robertson et al., 2022).Our results indicate that SSTR-related signaling pathways have enormous potential for antitumor immunity.Mounting data illustrate that the infiltration of immunocompetent cells may contribute to tumor development and recurrence as well as the determination of the immunotherapy response and clinical outcome (Gajewski et al., 2013;Han et al., 2022;Zou et al., 2023).SSTRs were considerably linked to six types of immune cell infiltrates: CD4 + T cells, CD8 + T cells, DCs, macrophages, neutrophils, and B cells.
SSTR2 is one of the most abundant SSTRs and a member of the GPCR family (Wu et al., 2020).Studies have shown that SSTR2 is associated with tumorigenesis in stomach and breast cancer and overexpressed in neuroendocrine tumors (Schulz et al., 2000;Skog et al., 2008;Tchoghandjian et al., 2016;Hope et al., 2018;Mohamed and Strosberg, 2019;Yin et al., 2020).SSTR2 reportedly interacts with the Wnt pathway protein Dvl1 in a ligand-independent way.SSTR2 is then targeted by Dvl1 for lysosomal degradation.SSTR2-targeted therapies may become more effective if this pathway is interfered with and SSTR2 expression in NETs is increased (Carr et al., 2023).Wildemberg showed that low SSTR2 expression can predict the failure of somatotropinomas to respond biochemically to SST analog treatment (Wildemberg et al., 2013).SSTR2 is an Epstein-Barr virus-induced druggable target in nasopharyngeal cancer, and Lechner and others demonstrated the preclinical effectiveness of targeted treatment (Lechner et al., 2021).The researchers found that the combination of SST and SSTR2 inhibits cytokine release from immune cells and impacts the tumor microenvironment (TME) (Patel, 1999;Cordova and Kurz, 2020).However, the relationship between SSTR2 and TME in COAD has not been reported.To further confirm the differential expression of SSTR2 in COAD and its association with immune cells.We collected 25 pairs of paraffin-embedded archived COAD specimens and matched adjacent normal tissues to confirm the differential expression of SSTR2 in COAD and its correlation with immune cells.In patients with COAD, SSTR2 expression is significantly downregulated.SSTR2 expression is also positively correlated with ITGAX, a gene associated with DCs.These results suggest that SSTR2 may exert antitumor effects by interacting with DCs in COAD.
Surgical treatment supplemented with postoperative adjuvant chemotherapy can treat mid-to early-stage COAD.Chemotherapy alone or combined with targeted therapy is the primary treatment for patients with advanced COAD.With only a 15% 5-year survival rate, patients with advanced COAD have a poor prognosis.Therefore, new treatments are urgently required to prolong patient survival (Auclin et al., 2017;Dekker et al., 2019).As a novel and potent anticancer therapy, immunotherapy is anticipated to become an alternative treatment option for CRC patients.The treatment of CRC has entered a new era with the advent of immunotherapy as a ground-breaking intervention following surgery in combination with radiotherapy, targeted therapy, and chemotherapy.ICI therapy is the most crucial immunotherapy in the fight against CRC (Zou et al., 2021;Pang et al., 2023).
Immunotherapies other than ICI have emerged rapidly in recent years, including CAR-T and oncolytic virus-based immunotherapy.The low immune cell infiltration level is the primary cause of the poor immune response to CRC (Hege et al., 2017;Zhao et al., 2022).The most crucial and effective therapeutic method for resolving this issue is CAR-T cell therapy.Zhang et al. (2017) performed a phase I clinical study of CAR-T therapy for CRC with high carcinoembryonic antigen expression.Of 10 patients, seven progressed while receiving prior treatment and remained stable.After CAR-T treatment, the condition of each remained unchanged.Two patients showed tumor shrinkage.In addition, Mandriani et al. showed that anti-SSTR CAR-T cells are highly effective target-dependent cytotoxic agents against a range of NET cell lines with different SSTR2/5 expression levels (Mandriani et al., 2022).This study aimed to determine whether abnormal SSTR expression affects the immunotherapy response of COAD.Our results suggest that SSTR2 may serve as an immunotherapeutic target in the treatment of COAD.The Lauss cohort showed a significant upregulation of SSTR2 expression in CRC patients receiving CAR-T cells.Differences were considered statistically significant.In patients with CRC receiving CAR-T and anti-PD-1/PD-L1 immunotherapy, SSTR2 could better discriminate between immune responders and non-responders (areas under the receiver operating characteristic curve, 0.933 and 0.782, respectively), and patients receiving CAR-T cells had a better OS, with statistically significant differences.By demonstrating a strong correlation between SSTR2 and immune molecules in CRC, our results support an immunoenhancing function of SSTR2.Additionally, our results suggest that SSTR2 contributes to tumor immunity.Therefore, it is a potential biological marker for anticipating the prognosis and effectiveness of immunotherapy in CRC patients.
Our study provides the first analysis of the relationship between SSTR family expression, tumor immune infiltration, and COAD prognosis to increase our understanding of the critical functions of these genes as drivers of tumor development and the immune system in patients with COAD.Our study identified the SSTR family as useful biomarkers and therapeutic targets that can be used to develop diagnostic and prognostic approaches to improve treatment outcomes.However, this study has some important limitations that require consideration.Furthermore, in vitro clinical studies are required to validate the likely processes underlying the action of multiple SSTR genes in COAD, the molecular links between them, and their clinical applications.

FIGURE 2
FIGURE 2 Protein expression levels of SSTR family members in COAD.(A-D) Protein expression levels of SSTR1-4 in COAD versus non-cancerous tissues.COAD, colon adenocarcinoma; SSTR, somatostatin receptor.

FIGURE 3
FIGURE 3 Relationship between stage and lymph node metastasis of COAD and SSTR family members.(A) Relationship between SSTR family mRNA expression levels and lymph node metastases of patients with COAD.Relationship between SSTR family mRNA expression levels and cancer stage of patients with COAD.(B) Relationship between SSTR family mRNA expression levels and cancer stage of patients with COAD.*p < 0.05, **p < 0.01, ***p < 0.001 compared with controls.COAD, Colon adenocarcinoma; SSTR, somatostatin receptor; UALCAN, University of Alabama at Birmingham Cancer Data Analysis Portal.

FIGURE 4
FIGURE 4 Prognostic value of mRNA expression levels of SSTR family members in patients with COAD.(A,B) The PrognoScan database was used to analyze the OS and DFS of the SSTR family in patients with COAD.COAD, colon adenocarcinoma; DFS, disease-free survival; OS, overall survival; SSTR, somatostatin receptor.

FIGURE 5
FIGURE 5 Genetic alterations and DNA methylation levels of the SSTR family members in patients with COAD.(A) DNA methylation changes in the SSTR family members in patients with COAD were assessed by the UALCAN database.(B,C) Summary of the rate of alteration for the SSTR family in the COAD (cBioPortal).*p < 0.05, **p < 0.01, ***p < 0.001 compared with control.COAD, colon adenocarcinoma; SSTR, somatostatin receptor; UALCAN, University of Alabama at Birmingham Cancer Data Analysis Portal.

FIGURE 7
FIGURE 7Association between SSTR mRNA expression with immune cell infiltration.(A-E) The TIMER2.0 database was used to evaluate the associations between SSTR family members and immune cell infiltration.SSTR, somatostatin receptor; TIMER, Tumor Immune Estimation Resource.

TABLE 1
Primer sequence for qRT-PCR.

TABLE 2
Clinicopathologic parameters of and SSTR family member expressions in COAD.

TABLE 3
Correlations between SSTR family member expressions and immune cell markers.

TABLE 3 (
Continued) Correlations between SSTR family member expressions and immune cell markers.