Aloe-emodin exhibits growth-suppressive effects on androgen-independent human prostate cancer DU145 cells via inhibiting the Wnt/β-catenin signaling pathway: an in vitro and in silico study

At the molecular level, several developmental signaling pathways, such as Wnt/β-catenin, have been associated with the initiation and subsequent progression of prostate carcinomas. The present report elucidated the anti-cancerous attributes of an anthraquinone, aloe-emodin (AE), against androgen-independent human prostate cancer DU145 cells. The cytotoxicity profiling of AE showed that it exerted significant cytotoxic effects and increased lactose dehydrogenase levels in DU145 cells (p < 0.01 and p < 0.001). AE also induced considerable reactive oxygen species (ROS)-mediated oxidative stress, which escalated at higher AE concentrations of 20 and 25 μM. AE also efficiently instigated nuclear fragmentation and condensation concomitantly, followed by the activation of caspase-3 and -9 within DU145 cells. AE further reduced the viability of mitochondria with increased cytosolic cytochrome-c levels (p < 0.01 and p < 0.001) in DU145 cells. Importantly, AE exposure was also correlated with reduced Wnt2 and β-catenin mRNA levels along with their target genes, including cyclin D1 and c-myc. Furthermore, the molecular mechanism of AE was evaluated by performing molecular docking studies with Wnt2 and β-catenin. Evidently, AE exhibited good binding energy scores toward Wnt2 and β-catenin comparable with their respective standards, CCT036477 (Wnt2 inhibitor) and FH535 (β-catenin inhibitor). Thus, it may be considered that AE was competent in exerting anti-growth effects against DU145 androgen-independent prostate cancer cells plausibly by modulating the expression of Wnt/β-catenin signaling.


Introduction
According to the recent data from the Global Cancer Observatory in 2020, prostate cancer patients contributed 7.3% to the global burden of cancer-related malignancies.Furthermore, prostate cancer was also correlated with 3.8% of cancer-associated fatalities globally (Global Cancer Observatory Factsheet, 2020).Prostate cancer is more frequent in older men during the latter half of their life, thus increasing its mortality and morbidity.In spite of substantial breakthroughs previously reported in the research on prostate carcinomas, the information elucidating its exact cause still remains elusive (Rawla, 2019).Nevertheless, certain important risk factors have been identified that regulate the onset and progression of prostate malignancies.Of these risk factors, impaired metabolism of androgen, ethnicity, and diet and mutated oncogenes are the most prominent ones.Indeed, subtle genetic mutations, even at a single amino acid or functional group level, initiate prostate carcinomas (Shukla and Gupta, 2004).Moreover, reports have further substantiated that impaired crosstalk between various signaling pathways is critical in increasing the invasiveness and proliferation of prostate cancer cells (Ehsani et al., 2021).Previous reports have indeed outlined that crosstalk between androgen receptor (AR), NF-κB, and Wnt/β-catenin signaling is imperative in increasing the metastasis and progression of prostate cancers (Lee et al., 2013;Koushyar et al., 2022).
Downstream molecules of the β-catenin pathway have also been reported to act as co-activators of the androgen receptor which itself is an oncogene associated with prostate carcinoma.Peptidyl-prolyl isomerase, also referred to as Pin1, is reported to influence the proliferation of prostate cancer cells (Chen et al., 2006).Moreover, it has also been reported that in the absence of androgens, the Wnt/βcatenin pathway modulates the switching on of the androgenmediated transcription pathway (Seo et al., 2017).Intriguingly, impaired androgen activity has also been reviewed to elevate Wnt/β-catenin signaling which culminates into the promotion of the androgen-independent proliferation of prostate cancer cells (Li et al., 2017;Wang et al., 2021).The crosstalk between β-catenin and AR often leads to a progression of prostate carcinomas, due to altered gene expression.Intriguingly, reports have shown that hyperactivated Wnt signaling results in escalated levels of βcatenin within the nucleus, leading to the expression of tumorpromoting genes (Zhan et al., 2017).A recent report correlated Wnt3 regulation with reduced β-catenin levels in the nucleus, leading to impaired cell cycle progression within prostate cancer cells (Dong et al., 2020a).Another report also showed that the modulation of Wnt/β-catenin by microRNA imparts therapeutic effects in prostate cancer (Cui et al., 2019).Indeed, these observations, along with others, make Wnt/β-catenin signaling a plausible target for the therapeutic intervention of prostate cancer (Wang et al., 2018;Cui et al., 2019;Liu et al., 2019).
Reports have substantiated that apoptotic cell death is indeed regulated by the crosstalk between Wnt/β-catenin and this has significant implications in several diseases (Ma et al., 2023).Apoptosis is undoubtedly a critical mechanism of immune responsiveness.Wnt/β-catenin signaling regulates several apoptosisrelated receptors including Fas and TRAIL, involved in the activation of the extrinsic pathway, as well as Bax/Bcl-2 involved in modulating intrinsic apoptosis pathways and caspases (Verbrugge et al., 2010).
Aloe-emodin (AE) is an anthraquinone that has been found abundantly in Aloe barbadensis miller, Cassia occidentalis, and Polygonum multiflorum.It is a well-known member of the anthraquinone family (Dong et al., 2020a).In addition to the various pharmacological properties, AE has also been known for its effects on cell cycle progression, apoptosis, immune signaling, and metastasis (Sanders et al., 2017;Dong et al., 2020b).Moreover, AE is also reported to induce apoptosis via intrinsic and extrinsic apoptotic pathways (Sanders et al., 2018).Nevertheless, the effects of AE on DU145 cells remain unexplored till date.Therefore, the investigators tried to study the anti-cancer effects of AE on DU145 cells and further explored AE-mediated modulatory effects on Wnt/β-catenin signaling.

Materials
Cell culture reagents, including Ham's F-12K media, fetal bovine serum (FBS), antibiotic-antimycotic cocktail, MTT stain, Hoechst 33342, and DCFH-DA dye, were procured from Sigma (St. Louis, MO, United States).RNase-A and the HiPurA TM Total RNA Miniprep Purification Kit used were from HiMedia, Mumbai, India.The DyNAmo ColorFlash SYBR Green Quantitative Polymerase Chain Reaction (qPCR) Kit was from Thermo Fisher Scientific, Waltham, MA, United States.The primers in the study were procured from IDT, Coralville, IA, United States.

In vitro studies 2.2.1.1 Cell culture
Androgen-independent human-derived prostate cancer DU145 cells and J774A.1 murine alveolar macrophages were procured from the National Centre of Cell Sciences, Pune, India.The cells were allowed to proliferate in RPMI 1640 and DMEM high-glucose media supplemented with 10% FBS and 1% antibiotic-antimycotic solution, both v/v.The cells were continuously provided a humidified atmosphere with 5% CO 2 at 37 °C.All the imaging reported in the present study was carried out using bright light and various fluorescent channels of the FLoid Imaging Station (Thermo Fisher Scientific, Waltham, Massachusetts, United States).

Cytotoxicity analysis
As described previously, AE-induced cytotoxic effects on DU145 cells were evaluated using MTT stain (Tiwari et al., 2021).AE was dissolved in 0.5% dimethyl sulfoxide (DMSO).Initially, 1 × 10 4 DU145 cells were exposed to 5, 10, 15, 20, and 25 μM concentrations of AE for 24 h; after exposure, 5 mg/mL MTT stain (10 μL) was added in cells treated with various AE concentrations and incubated for another 4 h.Eventually, the cells were analyzed for the formation of formazan crystal by measuring their intensity at 570 nm.The cytotoxicity of AE against DU145 cells was expressed as cell viability percentage (%) using the following formula.
Cells treated with 0.5% DMSO served as vehicle control and were subsequently used to compare the cytotoxicity of AE in DU145 cells.Furthermore, the effect of AE on J774A.1 cells was also evaluated by calculating the cell viability of J774A.1 cells using the same formula mentioned above.

Lactose dehydrogenase assay
The ability of AE to induce cytotoxicity against DU145 cells was also reaffirmed by lactose dehydrogenase assay (LDH) assay, as described previously (Ahmad et al., 2021).Initially, 5 × 10 3 DU145 cells were exposed to 5-25 μM concentrations of AE for 24 h.The levels of LDH in the supernatant of cells treated with varying AE concentration was determined colorimetrically using ELISA.100 μL of the reaction mixture was supplemented to the supernatant collected from respective AEtreated DU145 cells.The mixture was briefly incubated in the dark for an additional 30 min.The conversion of NAD + to NADH, characterized by the forming of formazan was quantified by recording the absorbance of the supernatant at 490 nm with the help of a spectrophotometer (Bio-Rad, Hercules, CA, United States).The formazan levels formed during the reaction were directly proportional to the amount of LDH released.Cytotoxicity was expressed in percentage (%) using the following formula.

AE-induced effects on nuclear morphology
Hoechst 33342 stain was used for qualitatively assessing the fragmentation of AE-treated DU145 cells as stated previously (Ahmad et al., 2021).For analysis, 5 × 10 3 DU145 cells were treated with varying stated AE concentrations for 24 h.After that, the media of each well were replaced with 2 μg/mL of Hoechst 33342 stain, and the plate was briefly incubated for 10 min in a humidified condition having 5% CO 2 .The characteristic blue fluorescence of the Hoechst 33342 stain in AE-treated DU145 cells was visualized, recorded, and compared with the control cells to assess changes in nuclear morphology.

AE-mediated effects on oxidative stress
Reactive oxygen species-mediated oxidative stress in AE-exposed DU145 cells was assessed through dichlorodihydrofluorescein diacetate (DCFH-DA) stain as per the previously described protocol (Alshehri et al., 2022).For this assay, approximately 1 × 10 3 DU145 cells were exposed to 5-25 μM concentrations of AE for 6 h.Subsequently, 10 μM DCFH-DA was supplemented to each for an additional 30 min, and the plate was left undisturbed in the dark.After incubation, the cells for each group were carefully washed using 1X phosphate buffer saline (PBS).The cells were then visualized and recorded for DCF-DA-mediated green fluorescence.The intensity of the DCFH-DA fluorescence in AEtreated DU145 cells (at the stated concentration) was compared with that of the untreated control DU145 cells.
For quantitative assessment, the same protocol was followed, except that approximately 1 × 10 5 DU145 cells were exposed to each stated concentration of AE for 6 h.Subsequently, after following nearly the same protocol, the cells were quantified for their DCFH-DA-related median fluorescence intensity (MFI).The DCFH-DA fluorescence percentage (%) was estimated by recording DCFH-DA absorbance at an excitation:emission wavelength of 485:528 nm using a Synergy H1 microplate reader (BioTek, Winooski, Vermont, United States).Comparisons were made between various AE-exposed DU145 cells and the untreated control to assess the relative changes in ROS levels.

Effect of ROS quencher
To reaffirm the efficacy of AE in inducing ROS-mediated oxidative stress, DU145 cells were pretreated with 10 mM N-acetyl-l-cysteine (NAC) for 2 h, followed by its exposure to AE at stated concentrations.After AE exposure for 6 h, the DU145 cells were washed and stained using DCFH-DA in the dark for 30 min.After that, the DCFH-DA fluorescence percentage (%) of each group was recorded using a spectrophotometer, as mentioned in the preceding section.

Assessment of caspase/s activity
The activity levels of caspase-3 and caspase-9 were evaluated colorimetrically in AE-treated DU145 cells following the manufacturer's instructions.Approximately 3 × 10 6 AE-treated DU145 cells were lysed in 500 μL chilled lysis buffer followed by a brief 10 min incubation.The suspension was centrifuged at 10,000 rpm for 1 min, and the supernatant was stored on ice.Subsequently, 500 μL of the collected cell lysate from each group was allowed to react with an equal volume of reaction buffer.Eventually, DEVD-pNA (4 mM) was added to each group, and the reaction was again briefly incubated for 10 min.Each treated and untreated group's absorbance was recorded subsequently at 405 nm.

Effect of caspase inhibitors
AE-induced cytotoxicity against DU145 was also confirmed through caspase inhibitors (Ahmad et al., 2022).DU145 cells were pretreated with Z-DEVD-FMK and Z-LEHD-FMK (50 μM each; caspase-3 and caspase-9 inhibitors, respectively) for 2 h.The cells were then retreated with AE, at the abovestated concentrations for 24 h, under optimum cell culture conditions.Finally, the viability of DU145 cells was estimated through the MTT test, as stated in Section 2.2.1.2.

AE-mediated effects on mitochondrial membrane potential (ΔΨm)
The AE-induced modulation of ΔΨm in DU145 was assessed colorimetrically using a mitochondrial membrane potential kit (Abcam; Cat.No. ab113852) following the manufacturer's instruction.AE-treated DU145 cells were briefly incubated for 15 min with 50 mM carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone.The cells were then briefly retreated with tetramethylrhodamine, ethyl ester (TMRE; 1 mM) for 30 min and then washed with PBS (0.2%).Finally, the absorbance of each group was recorded at an excitation:emission of 549:575 nm.

Cytochrome-c release assay
The cytosolic levels of cytochrome-c were quantified in AEtreated DU145 cells as per the protocol described previously (Tiwari et al., 2022). 1 × 10 6 DU145 cells were exposed to the abovestated AE concentrations for 24 h.The total protein content of DU145 cells from different groups was collected using T-PER reagent (Thermo Fisher Scientific, Waltham, MA, United States) through centrifugation (1,000 rpm at 4 °C for 10 min).Cytochrome-c levels within the cell lysate were quantified using an ELISA kit (Cat.No. KHO1051; Thermo Fisher Scientific, Waltham, MA, United States) by adhering to the manufacturers' protocol.

Estimation of cleaved poly(ADP-ribose) polymerase levels
AE-exposed DU145 cells were assessed for poly(ADP-ribose) polymerase (PARP) levels colorimetrically using a human-specific PARP ELISA kit (Cat.No. KHO0741, Thermo Fisher Scientific, Waltham, MA, United States) by adhering to the manufacturer's instruction.Different AE-treated and untreated groups' absorbance was recorded at 450 nm using a spectrophotometer (Bio-Rad, Hercules, California, United States).

qRT-PCR
qRT-PCR-based studies were subsequently undertaken to assess AE-induced modulatory effects on Wnt/β-catenin signaling and other important gene targets.During the analysis, the total RNA was isolated from 1 × 10 6 AE-exposed, and control DU145 cells, using a HiPurA TM kit (Thermo Fisher Scientific, Waltham, MA, United States).Next, cDNA was prepared through 2 μg of isolated RNA using a Verso cDNA synthesis kit (Thermo Fisher Scientific, Waltham, MA, United States) following the supplier's manual.The sequences of primers used in the study have been included in Table 1.The β-actin gene was used as a housekeeping gene during the study, and all the normalizations were performed against this gene.Data obtained were subsequently analyzed through the comparative CT method, and the fold change in gene expression was assessed through the 2−ΔΔCT method.

Statistical inferences
The data are the mean ± SEM of three discrete experiments performed at least thrice in triplicates.Data were analyzed through GraphPad Prism (Ver.5.0) using one-way ANOVA and Student's t-test as per applicability.Differences between the groups were inferred to be significant, when p < 0.05.* represents p < 0.05; **p < 0.01, and ***p < 0.001.

2.2.2
In silico studies 2.2.2.1 Docking studies AE was docked using AutoDock Vina 4 (Wang et al., 2011).The centroid of the target protein was chosen as the binding pocket coordinate, and a grid box was placed within a cubic box of magnitude ×40 40 × 40 Å.The optimal docking position was selected from nine poses, based on the interacting residues that formed hydrogen bonds with high binding affinity (kcal/mol).The protein-ligand interactions of the docked complexes were generated using PyMol (Trott and Olson, 2010).All the figures were generated using PyMol (Lill and Danielson, 2011).

AE impeded the growth of DU145 cells
To explore the plausible cytotoxic effects of AE against DU145 cells, an MTT assay was performed.It was observed that the growth and proliferation rate of the DU145 cell line was in inverse variation with the different concentrations of AE.Upon culturing of DU145 cells with AE, the growth of these cells was reduced to 88.80% ± 3.69% (5 μM), 67.75% ± 4.66% (10 μM), 46.73% ± 5.03% (15 μM), 28.92% ± 4.56% (20 μM), and 17.92% ± 2.73% (25 μM), as compared to unaltered viability in the control cells (Figure 1A).Our data, as shown in Figure 1B, exhibited that the IC50 values of AE was 12.47 ± 1.047 μM on DU145 cells, after treatment for 24 h.Therefore, based on the findings of this assay, further anti-cancer experimentation was performed.In addition, MTT assay was performed to investigate the effect of AE on normal murine macrophages J774A.1.The results showed that AE imparts insignificant cytotoxic effect on normal murine macrophages.

AE-induced nuclear fragmentation
To confirm that AE-induced cytotoxic effects were due to apoptosis induction, Hoechst 33342 staining was performed.Upon treatment  with varying concentrations of AE for 24 h, significant nuclear fragmentation was observed, as shown in Figure 2. The fluorescent micrographs revealed the presence of condensed and fragmented nuclei in AE-treated DU145 cells as compared to the untreated cells.

AE-mediated pro-oxidant activity
The intracellular production of reactive oxygen species (ROS) was assessed by using DCFH-DA staining.The fluorescent micrographs of AE-treated DU145 cells, shown in Figure 3A, were captured, and the dye intensity was found to be directly proportional to the amount of ROS generated in the cells.At the indicated concentrations of 5-25 μM AE, substantial augmentation of ROS was observed in DU145 prostate cancer cells.
Furthermore, ROS's intracellular generation was quantitatively assessed to validate our qualitative results.In the case of DU145 cells, the intracellular level of ROS was found to be 53.35% ± 2.99%, as compared to the untreated cells, at the concentration of 10 μM, which was followed by 80.42% ± 4.49% (15 μM), 123.22% ± 4.55% (20 μM), and 148.86% ± 4.44% (25 μM) as compared to the control cells (Figure 3B).These results , thus, suggested AE induced the elevation of ROS levels in a dosedependent manner.
Moreover, to confirm that AE-treated DU145 cells mediated the generation of ROS, the amount of ROS levels in prostate cancer cells was assessed in the presence of NAC, a well-known ROS inhibitor, followed by the treatment with AE.The results demonstrated that pretreatment with NAC (5 mM) completely abolished the elevated ROS levels within DU145 cells, which verified that AE could augment the generation of ROS in prostate cancer cells (Figure 3C).

Ameliorative effects of caspase inhibitors against AE-induced apoptosis
DU145 prostate cancer cells were treated with caspase inhibitors to ascertain that AE-mediated apoptosis induction was the result of caspase activation.The cells were pretreated with 50 µM Z-DEVD-FMK (a caspase-3 inhibitor) and Z-LEHD-FMK (caspase-9 inhibitor) for 2 h, followed by treatment with AE at the stated doses for 24 h.MTT assay was determined to measure the cell viability (Figures 4B,C).Pretreatment with caspase-3 and -9 inhibitors substantially inhibited the cytotoxic effects of AE on DU145 cells.

AE disrupted ΔΨm
Mitochondria are known for their contribution in inducing apoptosis.Dissipation of ΔΨm mediates apoptosis via the mitochondrial-dependent intrinsic apoptosis pathway.As demonstrated in Figures 5A,A reduction in NIR fluorescence was observed in AE-cultured DU145 after staining with Mito-NIR dye, implicating depolarization of mitochondria in comparison with the control where the ΔΨm was unaltered.Thus, AE treatment strongly altered the mitochondrial membrane potential (MMP) directly depending on AE concentration in prostate cancer DU145 cells.

AE mediated enhanced cytochrome-c release and PARP cleavage
To study the effect of AE on the release of cytochrome-c level in DU145 prostate cancer cells, an ELISA was performed.As demonstrated in Figure 5B, the treatment of DU145 cells with varying concentrations of AE increased the level of cytochrome-c in a dose-dependent trend.It was noted that AE treatment increased the level of cytosolic cytochrome-c by 5.66-folds at 25 μM concentration of AE.Therefore, AE-induced intrinsic or mitochondrial-dependent apoptosis via the release of cytochrome-c in the cytosol leads to apoptosis in the prostate cancer cells.
In addition, to find out whether caspase-mediated apoptosis in AE-treated prostate cancer cells was connected to PARP cleavage, cleaved PARP ELISA was performed on DU145 cells.As observed in Figure 5C, AE enhanced the level of PARP cleavage with increasing doses of AE in DU145 cells.The results demonstrated an increase in PARP cleavage levels by 2.77-folds at 25 μM concentration of AE.Therefore, AE-induced caspase-dependent apoptosis via PARP cleavage is a crucial attribute of apoptosis in prostate cancer cells.

Molecular docking results
The 2D and 3D structures of AE, FH535, CCT036477, Wnt2, and β-catenin are shown in Figure 7.As per the results of molecular docking analysis, it was demonstrated that the binding energy of AE toward Wnt2 and β-catenin was −7.0 and −8.0 kcal/mol, which were comparable to the binding energies of CCT036477 and FH535 (−6.9 and −7.7 kcal/mol) as shown in Figure 8.The residues   2.3 Å), which was involved in hydrogen bonding (Figure 11).Thus, AE is explored as a propitious natural compound with efficacy to Wnt/β signaling through molecular docking studies.The binding energies of AE with breast cancer targets (Wnt2 and β-catenin) and the interacting amino acids are summarized in Table 3.

Discussion
Despite the significant modern-day progress, the usage of present therapeutic modalities, including the standard radio and chemotherapeutics, needs to be improved by the systemic cytotoxicity and generation of resistance toward these therapies (Liu et al., 2021).This has necessitated the usage and exploration of efficacious natural compounds for their plausible anti-cancer role applicable to human use.Indeed, previous reports have shown that AE possesses a wide array of pharmacological attributes, including anti-bacterial and anti-microbial activities (Dong et al., 2020a).However, of all these attributes, the intrinsic anti-cancer efficacy of AE is of particular interest.Previously, the anti-cancer effects of AE have been reported in colon, breast, pancreatic, and lung cancer cells (Sanders et al., 2018).Indeed, it has been demonstrated that AE modulates key signaling pathways, including PI3K/Akt/ mTOR, ROS-JNK, MAPKs, Ras/ERK, and PKC (Acevedo- Duncan et al., 2004;Tu et al., 2016;Tseng et al., 2017;Dou et al., 2019;Shen et al., 2020).Furthermore, AE is also associated with regulating the expression levels of key genes, namely, c-Myc, NF-κB, casein kinase II, and ALP (Chen et al., 2014;Dong et al., 2020a).Nevertheless, the mechanistic insight into the functioning of AE in androgen-independent  Two-dimensional interaction complex of AE with Wnt2 protein as evaluated through AutoDock Vina.
Frontiers in Pharmacology frontiersin.org10 DU145 prostate cancer cells is still vague.The results from our study indicated that AE indeed exerted significant cytotoxic effects on DU145 cells.Based on the observations of MTT and LDH release assay, the present study showed that AE impeded the proliferation of DU145 cells in direct proportion to the concentration of AE.
To gain a better insight into the mechanistic anti-cancer effects of AE, its functioning at molecular levels impeding the viability of DU145 cells was subsequently investigated.Oxidative stress mediated by ROS plays a major role in varying the redox state of cancer cells and is, thus, an important target for various anti-cancer therapeutics (Liou. and Storz, 2010).ROS levels subsequently serve Two-dimensional interaction complex of the CCT036477 inhibitor with the Wnt2 protein complex as evaluated through AutoDock Vina.

FIGURE 11
Two-dimensional interaction complex of AE with the β-catenin protein complex as evaluated through AutoDock Vina.Thr330,Tyr331,Glu334,Tyr333,Leu519,Val251,Lys292,Thr289,Pro521,Glu479,Ala478,Glu477,Gln476,and His475 Frontiers in Pharmacology frontiersin.org 11 Hussain et al. 10.3389/fphar.2023.1325184as a critical impetus for activating various signaling pathways in regulating key intracellular processes such as inflammation, cell proliferation, migration, and apoptotic cell death (Ye et al., 2016).Due to its important role in cancer cells, the investigators studied AE-mediated effects on ROS levels in DU145 cells.The results indicated that upon AE exposure, intracellular ROS levels escalated considerably in the prostate cancer cells, which was characteristically proportional to AE concentration.This escalation of ROS levels could further be correlated with the activation of mitochondriadependent apoptotic cell death.Intriguingly, AE exposure in ROS quencher pretreated DU145 cells significantly reduced ROS generation, which was coherent with our observation that AE induced the escalation of ROS generation in DU145 cells.
Being an important member of the cysteine protease family, caspases play prerequisite roles in modulating the apoptotic pathway (McIlwain et al., 2013).Caspase activation during the onset of apoptosis is primarily mediated by the death-receptor pathway, where caspase-8 is established to provide key impetus.Furthermore, caspase-8 activation results in the downstream impelling of the mitochondria-dependent apoptotic pathway due to the concomitant cytosolic accumulation of cytochrome-c (Tummers and Green, 2017).Importantly, these pathways in their activated state led to the activation of caspase-3 by cleavage-dependent pathways.The observations from the present study indicated increased ROS levels concomitantly following the onset of apoptotic pathways after AE exposure, dissipated ΔΨm followed by increased caspase activities, and modulated expression of apoptosis-associated proteins.Thus, it can be inferred that AEinduced apoptotic cell death in DU145 cells was significantly modulated by caspase activation.Intriguingly, AE-mediated cytotoxicity was considerably alleviated in the presence of caspase-3 and -9 inhibitors, indicating the relevance of these caspases in impelling apoptotic cell death.
In mammalian cells, mitochondria are important sites mediating ROS generation, resulting in dysfunctional mitochondria and the dissipation of ΔΨm, leading to the discharge of cytochrome-c from mitochondria (Zorova et al., 2018).Indeed, in the present investigation, a significant decline in ΔΨm was observed in DU145 cells after exposure to AE.This observation supported the notion that dissipated ΔΨm activates caspase-3 by functionally activating endonuclease.This dissipation of ΔΨm after AE exposure corroborated with the ROS levels and mitochondriadependent apoptosis.Intriguingly, initiating apoptotic cell death in cancers is considered an attractive target for therapeutic intervention (Arfin et al., 2021).Among various other characteristics, DNA fragmentation and chromatin condensation represent the major characteristic during the onset of apoptosis.Our observations were indeed in line with the stated notion.During the study, it was inferred that AE exposure resulted in the apoptotic cell death of DU145 cells on the basis of observations recorded during the DAPI assay.Furthermore, this notion was also corroborated by our findings during PARP cleavage and cytochrome-c release assay in DU145 cells.
Developmental signaling pathways such as the Wnt pathway are indispensable in regulating proliferation, the initiation of drug resistance, and pathogenesis in prostate carcinoma (Seo et al., 2017).Reports have substantiated that the abnormal expression of Wnt receptors, their ligands, and inhibitors plays a significant role in the onset of prostate cancer (Zhu et al., 2020).The present study also observed that AE-induced impaired DU145 cell growth correlated with altered Wnt/β-catenin signaling.The study showed that AE reduced the Wnt2 and β-catenin expression.Intriguingly, AE also succeeded in lowering cyclin D1 mRNA, which subsequently promotes the progression of cells through the G0/G1 checkpoint (Behrens, 2000;Fu et al., 2004).Similarly, survivin is another important downstream target of Wnt/β-catenin signaling and is also an important member of antiapoptotic proteins.
c-Myc is a multifaceted oncogene that regulates various tumorigenesis-, proliferation-, and cell growth-related processes in several carcinomas.Among these, promoting the progression of cells to different phases of cell cycle is the most important biological function of c-Myc.Indeed, AE also exhibited its competence in deflating the mRNA expression of c-Myc in DU145 cells.This finding subsequently indicated that the Two-dimensional interaction complex of the FH535 inhibitor with the Wnt2 protein complex as evaluated through AutoDock Vina.
Frontiers in Pharmacology frontiersin.orgimpairment of c-Myc levels could plausibly be related to the onset of apoptotic cell death and arrest of cell cycle progression in prostate carcinoma.In addition, in vitro findings were subsequently corroborated by performing in silico studies.AE was docked with Wnt2 and β-catenin at the binding energies of −7.0 and −8.0 kcal/ mol, which was comparable to the binding energies of CCT036477 and FH535 (−6.9 and −7.7 kcal/mol) (Figure 12).Therefore, correlation was performed between in silico findings and qPCR results so as to establish that AE downregulated the Wnt/β-catenin signaling pathway and exhibited strong binding affinity toward Wnt2 and β-catenin.Furthermore, these results are in support of real-time PCR results and provide a strong rationale that AE significantly inhibited the Wnt/β-catenin signaling in prostate cancer cells.In total, in the current investigation, it was reported that AE modulated the Wnt/βcatenin pathway and inhibited the growth of androgenindependent prostate cancer cells.Thus, the findings from this research could provide a novel insight into the involvement and the underlying molecular mechanism of AE in prostate cancer.

Conclusion and future perspective
The present investigation indicated the anti-proliferative efficacy of AE by modulating the important components and targets of Wnt/β-catenin signaling in androgen-independent DU145 prostate cancer cells.AE was also competent in instigating apoptotic cell death, subsequently exerting anti-cancer effects on DU145 cells.The observations from the present report indicate the plausible usage of AE as an anti-cancer intervention in prostate cancer treatment.AE can also be further investigated as plausible adjunct therapeutics with the existing chemotherapeutic drugs in treating prostate cancer.Moreover, AE was mostly tested on a limited range of cancer cell lines, so its spectrum of activity needs to be expanded.In certain instances, the effectiveness of AE was limited by its poor bioavailability.Therefore, researchers should lay emphasis not only on the efficacy of compound, which is of substantial interest, but also on effective drug delivery systems aimed at overcoming the pharmacokinetic issues along with studying derivatives with a high degree of biological efficacy and availability.

FIGURE 8
FIGURE 8Two-dimensional and three-dimensional molecular structures of various chemicals and proteins involved in the present investigation.

TABLE 1
List of primer sequences used for mRNA expression analysis.

TABLE 3
Binding energies of AE with Wnt2 and β-catenin in comparison with their respective standards CCT036477 and FH535.