Microbial Induction of Immunity, Inflammation, and Cancer

The human microbiota presents a highly active metabolic that influences the state of health of our gastrointestinal tracts as well as our susceptibility to disease. Although much of our initial microbiota is adopted from our mothers, its final composition and diversity is determined by environmental factors. Westernization has significantly altered our microbial function. Extensive experimental and clinical evidence indicates that the westernized diet, rich in animal products and low in complex carbohydrates, plus the overuse of antibiotics and underuse of breastfeeding, leads to a heightened inflammatory potential of the microbiota. Chronic inflammation leads to the expression of certain diseases in genetically predisposed individuals. Antibiotics and a “clean” environment, termed the “hygiene hypothesis,” has been linked to the rise in allergy and inflammatory bowel disease, due to impaired beneficial bacterial exposure and education of the gut immune system, which comprises the largest immune organ within the body. The elevated risk of colon cancer is associated with the suppression of microbial fermentation and butyrate production, as butyrate provides fuel for the mucosa and is anti-inflammatory and anti-proliferative. This article will summarize the work to date highlighting the complicated and dynamic relationship between the gut microbiota and immunity, inflammation and carcinogenesis.

intestinal inflammation, and prime the innate and the adaptive arms of the immune system (Vaarala, 2003). Aberrations in the biodiversity of enteric microbiota can contribute to individual differences in immunologic behavior during and subsequent to childhood. Western nations, for instance, have experienced consistent increases in the incidence of allergic diseases in the past few decades, which may be due to a lack of microbial exposure during infancy -the "hygiene hypothesis" -or adoption of a Western diet (Strachan, 1989;Wang et al., 2008). A synchronous decrease in the incidence of infectious diseases in developed countries has occurred subsequent to the implementation of antibiotics, vaccination, and improvements in hygiene (Bach, 2002).
Mounting evidence suggests that diet and the microbiota, independently or in conjunction, influence the risk of developing atopic disease. The constitution of enteric microbiota may be a consequence of one's country of origin. Infants living in developing countries have been shown to be colonized at younger ages with fecal bacteria and have more rapid transfer of enteric microbial strains than infants living in developed countries (Adlerberth et al., 1998). Diversity is considerably greater in rural African than European children (Figure 1), with a predominance of resistant polysaccharide hydrolyzers (Prevotella and Xylanibacter) and relative absence of inflammatory Enterobacteriaceae (De Filippo et al., 2010). Compared to children without atopic illnesses, microbial species of children who manifest atopic sensitization demonstrate a reduced ratio of bifidobacteria to clostridia when they are infants (Kalliomaki et al., 2001a). Important determinants of enteric microbiotic composition in infants appear to be the mode of delivery, maternal diet, type of infant feeding (breast milk or formula), gestational age, infant hospitalization, antibiotic use by the infant, and the presence of siblings (Penders et al., 2006). One

IntroductIon
The distal intestine is populated by a stunning quantity of bacteria, comprised of relatively few phyla that are highly diverse at the species level. This heterogeneous composition presents a vibrant metabolically active life-form, sharing the same habitat as our enteric organs. Realistically, enteric microbiota are a continuum, with members existing somewhere along the spectrum between symbiosis and pathogenicity. As the microbiota co-evolved with humans, they have provided us with genetic and metabolic characteristics that we have not had to develop on our own, such as enabling us to be able to harvest otherwise inaccessible nutrients and synthesizing vitamins (O'Keefe et al., 2009). Resistant starch and non-starch polysaccharides that evade human digestion provide energy for commensal bacteria. In turn, microbes metabolize these undigested nutrients to produce short-chain fatty acids (SCFA), including butyrate, the primary nourishment for colonocytes which possesses potent anti-proliferative and anti-inflammatory properties (Pryde et al., 2002). Alternatively, enteric microbes may enhance disease pathogenesis at the cellular and molecular level via pro-inflammatory mechanisms ( Table 1).
promoted cellular proliferation by triggering mitogen-activated protein kinases which can increase the incidence of cell transformation as well as the rate of acquiring genetic mutations (Baron and Sandler, 2000;Biarc et al., 2004). Pro-inflammatory cytokines act as tumor promoters by regulating cytokines, chemokines, adhesion molecules, and angiogenesis (Coussens and Werb, 2002). A recent study of multiple intestinal neoplasia mice showed that infection with enterotoxin-producing Bacteroides fragilis organisms that produce inflammatory diarrhea in humans triggered colitis and strongly induced colonic tumors (Wu et al., 2009). Clinical support for the importance of chronic inflammation is provided by the observation that colon cancer risk is increased five-fold in patients with chronic ulcerative colitis (Ekbom et al., 1990). Furthermore, the risk of cancer in inflammatory bowel disease (IBD) is considerably higher if the disease affects the colon, rather than the small intestine, indicating that the carcinogenic effects of bacteria and inflammation may be additive. Finally, it has been clearly established that the use of anti-inflammatory medications suppresses colon cancer risk (Baron and Sandler, 2000).
Substantial epidemiological evidence also supports a primary role for diet in the genesis of colorectal carcinoma (Doll and Peto, 1981). Comparative studies from high and low risk populations indicate that elevated intake of red meat and animal fat increase colon cancer risk, while greater consumption of fiber reduces risk. The effect of diet on colonic carcinogenesis may be mediated by the microbiota, which can either maintain colonic health or promote chronic inflammation (O'Keefe et al., 2009). Fermentation of indigestible carbohydrate, such as fiber and resistant starch, maintains colonic health by producing SCFA such as acetate, propionate, and butyrate. Unlike most other cells in the body, colonocytes use butyrate as their preferred energy source. Furthermore, butyrate appears to be fundamental in the maintenance of cellular homeostasis and a normal colonocyte phenotype (Topping and Clifton, 2001). SCFAs have anti-inflammatory effects, either directly by regulating the release of prostaglandin E2, cytokines, and chemokines from human immune cells (Cox et al., 2009), or indirectly by their ability to support the growth of probiotic study comparing the enteric microbial species constitutions of 7-year-old children noted that those delivered by Cesarean section had significantly lower quantities of clostridia and bifidobacteria (Salminen et al., 2004). Additionally, infants who are treated frequently with antibiotics have a heightened likelihood of developing asthma (Marra et al., 2009).
Breast feeding is associated with a diminished risk of atopic diseases, and alteration of the mother's diet to avoid n−6 saturated fat and include supplementation with Lactobacillus rhamnosus GG (ATCC 53103), has been shown to reduce risk by 50% (Kalliomaki et al., 2001b). Diet in early life may also affect the likelihood of developing allergic illnesses. For instance, infants fed a strictly organic diet were found to have a significantly lower likelihood of developing eczema (Kummeling et al., 2008). Dietary lipids, particularly n−6 long-chain polyunsaturated fatty acids, have a pro-inflammatory effect through the increased synthesis of prostaglandin E2; their consumption can contribute to the development of allergies and asthma (Black and Sharpe, 1997). N−3 polyunsaturated fatty acids, on the other hand, possess a demonstrated ability to decrease the inflammatory response via their reduction in prostaglandin E2 levels (Kobayashi et al., 2006). Diet may, thus, affect the development of allergic disease both directly by its effect on inflammation and indirectly through its influence on the composition and activity of the microbiota.

colon cancEr
Robust experimental and clinical evidence demonstrates that chronic inflammation increases the risk of neoplastic transformation (Ekbom et al., 1990;Baron and Sandler, 2000;Coussens and Werb, 2002;Garcea et al., 2005). Not surprisingly, studies have linked colonic carcinogenesis to chronic inflammation generated by "pathogenic" bacteria. Although specific bacterial species have yet to be proven to be the cause of colon cancer, the minor gut colonizer Streptococcus bovis has been implicated in some clinical studies. Experimental investigations into its carcinogenic potential showed that bacterial proteins upregulated COX-2 production and Table 1 | Cellular and molecular evidence for the role of the microbiota in inflammation and disease.

Finding Reference
The differentiation of IL-17-producing T-helper cells in the lamina propria of the small intestine requires commensal cytophagaflavobacter-bacteroidetes bacteria and can be inhibited with antibiotics Ivanov et al. (2008) A microbial factor known as polysaccharide A suppresses pro-inflammatory interleukin-17 production by intestinal immune cells and protects from inflammatory-mediated illnesses via functional requirement for interleukin-10-producing CD4+ cells Mazmanian et al. (2008) IL-2 deficient, germ-free rats develop colitis due to a non-pathogenic E. coli mpk strain Waidmann et al. (2003) Enteric bacteria influence outcome of systemic immune responses by determining the ratio of Th1 to Th2 effector cells Mazmanian et al. (2005) Mutations in genes involved in bacterial peptidoglycan recognition (NOD2/CARD15) are risk factors for Crohn's disease Abraham and Cho (2009) The probiotic bacterial proteins p75 and p40 are capable of promoting intestinal epithelial homeostasis and significantly reduce TNF-induced colon epithelial damage Yan et al. (2007) Lactobacillus casei has been shown to attenuate the severity of experimental colitis by upregulation of ICAM-1 expression and leukocyte recruitment Angulo et al. (2006) SCFAs have anti-inflammatory effects by regulating the release of prostaglandin E(2), cytokines, and chemokines from human immune cells Cox et al. (2009) SCFAs support the growth of probiotic species bifidobacteria and lactobacilli Delcenserie et al. (2008) Butyrate maintains colonic motility by modifying histone acetylation in the colonic myenteric plexus Soret et al. (2010) species, such as bifidobacteria and lactobacilli (Wang et al., 2002;Delcenserie et al., 2008). SCFAs also possess immune-modulating and anti-inflammatory actions by binding certain G-coupled receptors which may help to stimulate the normal resolution of inflammatory responses in colonocytes (Maslowski et al., 2009). Colonic blood flow as well as fluid and electrolyte uptake are also enhanced by SCFA production (Topping and Clifton, 2001). Butyrate, in particular, can modulate inflammation and affect tumorigenesis. Evidence shows that risk reduction may be related to the effects of butyrate on activating the apoptosis cascade and inducing growth arrest of tumors by histone hyperacetyla-tion (Avivi-Green et al., 2000). In vitro, butyrate demonstrates numerous additional anti-inflammatory properties, including decreasing the effects of pro-inflammatory cytokines and their expression by inhibiting NF kappa B activation and abolishing lipopolysaccharide (LPS)-induced expression of cytokines by peripheral blood mononuclear cells (Segain et al., 2000). Butyrate also inhibits the in vitro preneoplastic hyperproliferation-induced tumor promoters and arrests the growth of neoplastic colonocytes (Pryde et al., 2002). A fascinating recent study provides experimental evidence that butyrate maintains colonic motility, and therefore "housekeeping," by modifying histone acetylation in circulation of primary bile acids, such as cholic acid and chenodeoxycholic acid, which are mostly reabsorbed, although a fraction reaches the colon -and this quantity increases on a high fat diet (Reddy, 1981). If the colonic microbiota contains 7α-dehydroxylating bacteria (chiefly clostridial species), the primary bile acids are converted to the secondary bile acids, namely deoxycholic acid (DCA) and lithocholic acid, which are well recognized co-carcinogens with lithogenic properties (Nagengast et al., 1995;Ridlon et al., 2006). Higher fecal and plasma levels of DCA have been observed in patients with colon cancer (Hori et al., 1998), and our studies have shown that levels were considerably higher in healthy, high risk African Americans compared to low risk healthy rural Africans (Ou et al., 2011). Our recent studies have confirmed that 7α-dehydroxylating bacteria are less common in developing countries, where gallstones and colon cancer are rare (Wells et al., 2000;O'Keefe et al., 2007). Figure A1 displays the relationship between food, the enteric microbiota, and the intestinal mucosa in regards to macronutrient metabolism and inflammation.
Finally, an abundant supply of colonic carbohydrate residues can stimulate the synthesis of vitamins by the resident microbiota. In our studies in rural Africans, we measured constituents of colonic evacuants following polyethylene glycol (PEG) consumption (O'Keefe et al., 2007). As expected, total colonic SCFA and butyrate were significantly higher in rural Africans than in Caucasians and African Americans. More surprising was that colonic folate and biotin content was similar in all three groups despite considerably lower dietary intake in Africans. Considering that content was higher than normal dietary intake, this indicated avid net synthesis by the microbiota. Folate is synthesized by some strains of specific microbes, e.g., bifidobacteria, from glycolysis and tricarboxylic acid cycle intermediates (Watson, 2006). Studies have shown that increasing colonic carbohydrate, in the form of oligosaccharides or resistant starch, enhances the production rate of folate by microbiota (Pompei et al., 2007). The importance of luminal folate and biotin is evident in recent studies demonstrating that active colonic mucosal transport mechanisms exist for these vitamins and that both vitamins play key roles in DNA synthesis and repair (O'Keefe et al., 2009).

Inflammatory bowEl dIsEasE
Inflammatory bowel disease comprises two distinct, but overlapping, entities -Crohn's disease and ulcerative colitis. There are approximately 1.4 million people living in the United States with IBD and most cases are diagnosed in individuals in their teens or twenties (Loftus Jr. et al., 2002). The etiology of IBD has been described as on-going intestinal mucosal inflammation that hinges on microbial or environmental factors and immune deficiencies in genetically predisposed individuals (Abraham and Cho, 2009). Etiologically, immune system dysfunction, an abnormally permeable mucosal barrier, or imbalances in beneficial and harmful species of enteric bacteria are hypothesized as contributing to IBD risk. Clinical and epidemiologic data demonstrate that a network of inflammatory components interacts to incite IBD (Abt and Artis, 2009). Examples of the influence of microbially perpetuated inflammation include the association of IL-10 mutations with ulcerative colitis (Franke et al., 2008) the colonic myenteric plexus, leading to an increased proportion of cholinergic neurons and accelerated colonic transit (Soret et al., 2010).
The negative side of fermentation is the production of hydrogen. Excess hydrogen can damage living cells by impairing NAD regeneration and inhibiting respiration (Gibson et al., 1993). This would not only injure the colonic mucosa (in the extreme case causing pneumatosis intestinalis), but would suppress the bacterial population size, thereby inhibiting its potential to feed itself and the colonic mucosa. The microbiota have evolved to circumvent this problem by expanding their diversity to contain organisms -archaeal hydrogenotrophic methanogens (e.g., Methanobrevibacter smithii) -specialized to consume hydrogen to produce the non-cytotoxic end-product methane (Wolin, 1976). The net effect is an enormous increase in the generation capacity of SCFAs from complex carbohydrates which ensures the health of both the microbiota and the colonic epithelium. As evidence, we observed that methanogenesis was considerably higher in low cancer risk rural Africans consuming high complex carbohydrate diets compared to high risk Americans who eat little fiber (O'Keefe, 2001).
Industrialized man's diet has changed from containing mainly unprocessed vegetables and grains to favoring meat, animal products, and refined cereals. This evolution reduces the quantity of complex carbohydrates or fiber that reaches the colon and alters the composition of the residue to contain more sulfur. The alteration in colonic milieu affects the diversity of the microbiota as sulfate stimulates the alternative growth of sulfur-reducing bacteria (SRBs, e.g., Desulfovibrio vulgaris), another form of hydrogenotrophic bacteria, which outcompete methanogens for hydrogen under these conditions (Gibson et al., 1988). Unfortunately, the metabolic end-product of SRBs, hydrogen sulfide (H 2 S), differs from methane, being inflammatory and genotoxic. H 2 S impairs cytochrome oxidase, suppresses butyrate utilization, inhibits the synthesis of mucus and the methylation of DNA (Gibson et al., 1993), and is genotoxic via the generation of free radicals (Attene-Ramos et al., 2007). Christl et al. (1992) demonstrated that 70% of British fecal samples contained SRBs and the remainder produced methane, whereas the reverse was the case with rural black South Africans (Gibson et al., 1988). The link to diet was illustrated by further studies showing that a high meat diet increased fecal sulfide (Magee et al., 2000), and increasing colonic sulfide suppressed methanogenesis (Christl et al., 1992). The clinical significance of this finding is exemplified by observing that colonic overgrowth with SRBs exists, together with suppression in butyrate production in patients with ulcerative colitis (Roediger, 1993), another condition that increases colon cancer risk. Interestingly, ulcerative colitis is also rare in native Africans (O'Keefe et al., 1983). While SRBs play an essential role in the external environment by suppressing the release of methane gas -one of the "greenhouse" gases -from sewers and marshes into the atmosphere (Guisasola et al., 2008), it seems that humans, unlike the carnivores, have had insufficient exposure time to a westernized diet to evolve mechanisms for preventing H 2 S injury.
A high meat diet is usually associated with elevated fat intake, and fat itself has been shown to influence cancer risk (Ferguson, 2010). Fat stimulates the synthesis and enterohepatic and elevated levels of mucosal IgG directed against the normal microbiota in IBD patients (Cummings et al., 2003). Additionally, mutations in genes involved in bacterial peptidoglycan recognition (NOD2/CARD15) are known risk factors for Crohn's disease (Abraham and Cho, 2009) and recent studies have shown that the microbiota can mediate the effects of these genetic defects (Rogler, 2010).
Rates of IBD in modernized nations are rising. Between the 1950s and 1990s, the incidence of Crohn's disease nearly tripled in Northern Europe (Farrokhyar et al., 2001). The "hygiene hypothesis" has again been indicted (Strachan, 1989). This hypothesis posits that the growing reliance on antibiotics and the sometimes obsessive focus on maintaining an anti-bacterial environment for children may be triggers for IBD on a population level. Studies of migrants, for instance, have shown that relocating from a developing to a developed nation increases the risk of IBD and risk is greatest if relocation occurs during infancy or early childhood (Bernstein and Shanahan, 2008). Regional differences in rates of IBD indicate that its etiology is exceptionally complex, with rates in the southern United States being lower than other US regions, but those of Olmstead county (Minnesota) being more similar to rates in Wales (UK) rather than neighboring Canadian provinces (Bernstein et al., 2006;Gunesh et al., 2008). The incidence of IBD in African and Asian countries, such as Korea, is only a fraction of what it is in the United States and the United Kingdom (Yang et al., 2008).
Clinical observations of IBD patients and animal models highlight the role of bacteria, viruses, and mycobacteria in intestinal inflammation, although no specific microbe or profile of microbes have to date been proven to be causative and it must be remembered that the disease itself and its treatment may also disturb the microbiota. Murine models of colitis require bacteria for inflammation to occur, while other animal models have shown that antibiotics that target anaerobes and Gram-positive organisms such as enterococci are protective against ulcerative colitis -particularly when they are administered prior to the induction of inflammation (Cummings et al., 2003). Antibiotics can also be effective treatments for some IBD patients (Elson et al., 2005). IL-2 deficient, germ-free rats develop colitis due to a non-pathogenic E. coli mpk strain but not with exposure to B. vulgatus (Waidmann et al., 2003).
Dysbiosis may also play a role in the etiology and severity of IBD, as some patients demonstrate lower enteric bacterial diversity with reduced proportions of butyrate-producers (Frank et al., 2007) and "protective" bacteria such as lactobacilli and bifidobacteria (Eckburg and Relman, 2007). This suggests that the mucosa is "at risk," exemplified by the heightened incidence and severity of Clostridium difficile colitis among IBD patients both in hospitalized and outpatient settings (Ananthakrishnan et al., 2009;Clayton et al., 2009). C. difficile thrives in a dysbiotic, "permissive," environment and is suppressed by metabolically active butyrate-producers. However, butyrate deficiency may not provide the whole explanation as there is evidence for decreased butyrate utilization in ulcerative colitis and restoration of butyrate-producers may not reverse the inflammation (Thibault et al., 2010). Other microbial products may better explain the ability of butyrate-producers contained within Clostridium cluster IV and XIVa to decrease intestinal inflammation and necrosis in the mouse model (Sokol et al., 2008). Sokol et al. (2008) showed that the supernatant of a culture of a member of cluster IV, Faecalibacterium prausnitzii, reduced colitis and mortality in the mouse model, even if administered orally or intraperitoneally.
Dietary factors may also influence risk of IBD. A Japanese study found a strong correlation between increased intake of animal protein and n−6 polyunsaturated fatty acids, and decreased intake of n−3 polyunsaturated fatty acids over the course of 20 years with Crohn's disease (Shoda et al., 1996). IBD is also more likely to occur following a bout of acute gastroenteritis. Researchers in Spain studied 43,013 patients aged 20-74 years who had experienced an episode of acute infectious gastroenteritis and found that the estimated incidence rate of IBD was 68.4 per 100,000 person-years after an episode of gastroenteritis but 29.7 per 100,000 person-years in an age-, sex-, and calendar-matched controls (Garcia Rodriguez et al., 2006). There was an especially greater risk of developing Crohn's disease during the first year after the infective episode (hazard ratio, 6.6; 95% CI, 1.9-22.4) although confounding of Crohn's symptoms with a bout of gastroenteritis might explain this observation. IBD studies also note that patients have greater risk of developing gastrointestinal infections; an IBD twin study noted greater rates of self-reported enteric infections among patients than their non-affected twin . Nonetheless, environmental triggers could be as important as genetic ones in the etiology of IBD. Studies of monozygotic twins in which only one twin is affected with IBD note that the unaffected twin may have altered mucosal glycosylation, indicative of "preinflammatory" NFkappaB activation which would be acquired rather than inherited (Bodger et al., 2006).

conclusIon
Enteric organisms share a common environment with the mucosa and have evolved in tandem to ensure survival of what has been described as the "superorganism." In health, the microbiota prevents overgrowth with pathogens and simultaneously educates the gut immune system in immunotolerance and defense. Disturbance of the microbiota by diet, hygiene, antibiotics, or disease leads to "dysbiosis," increasing the potential for mucosal injury and inflammation which, with time, may trigger a variety of mucosal and systemic diseases depending upon the host's genetic susceptibility. The final result of the soluble protein products of inflammatory cells is tissue damage, which can manifest in a variety of disease states, including eczema, colonic neoplasia, or IBD. Each of these entities requires a number of interactive factors to evolve and a prerequisite genetic susceptibility. Reproductive and lifestyle factors in developed nations, including advancements in public healthy hygiene measures, could help foster an environment that diminishes the protective effects of the enteric microbiota. Nonetheless, we can make a concerted effort to write fewer antibiotic prescriptions for infants, support breastfeeding for new mothers and discourage elective C-sections unless conditions necessitate them. Additionally, education about protective dietary constituents should be embraced by clinicians. For instance, encouraging patients to include ample fiber or complex carbohydrates in their diet, supplementing with probiotics, choosing "healthy" n−3 fats over pro-inflammatory n−6 fats, and limiting meat consumption, may optimize health and longevity.