pCO2 and pH regulation of cerebral blood flow

CO2 serves as one of the fundamental regulators of cerebral blood flow (CBF). It is widely considered that this regulation occurs through pCO2-driven changes in pH of the cerebral spinal fluid (CSF), with elevated and lowered pH causing direct relaxation and contraction of the smooth muscle, respectively. However, some findings also suggest that pCO2 acts independently of and/or in conjunction with altered pH. This action may be due to a direct effect of CSF pCO2 on the smooth muscle as well as on the endothelium, nerves, and astrocytes. Findings may also point to an action of arterial pCO2 on the endothelium to regulate smooth muscle contractility. Thus, the effects of pH and pCO2 may be influenced by the absence/presence of different cell types in the various experimental preparations. Results may also be influenced by experimental parameters including myogenic tone as well as solutions containing significantly altered HCO3− concentrations, i.e., solutions routinely employed to differentiate the effects of pH from pCO2. In sum, it appears that pCO2, independently and in conjunction with pH, may regulate CBF.

A major physiologic regulator of cerebral blood flow (CBF) is arterial blood pCO 2 , with hyper-and hypo-capnia resulting in dilatation and contraction of the cerebral vasculature, respectively (Harper and Bell, 1963). The mechanism underlying this regulation appears independent of the decreased and increased arterial pH levels accompanying the elevated and lowered pCO 2 , respectively, since CBF remains unchanged following metabolic acidosis and alkalosis (Lambertsen et al., 1961;Harper and Bell, 1963;Severinghaus and Lassen, 1967;Nau et al., 1999;Anderson and Meyer, 2002). Rather, findings suggest that CBF is regulated by changes in pH of the cerebral spinal fluid (CSF) as the result of the rapid equilibration between CO 2 in the arterial blood and CSF (Lambertsen et al., 1961;Harper and Bell, 1963;Severinghaus and Lassen, 1967;Lassen, 1968). The lowered/elevated pH in the CSF then acts directly on the vasculature to cause relaxation and contraction, respectively (Lambertsen et al., 1961;Harper and Bell, 1963;Severinghaus and Lassen, 1967;Lassen, 1968). Thus, the action of pCO 2 on the vasculature is restricted to that of altering CSF pH, i.e., is void of other indirect effects as well as direct effects (Lambertsen et al., 1961;Harper and Bell, 1963;Severinghaus and Lassen, 1967;Lassen, 1968).
Evidence in support of this proposal includes demonstrations in cat and rat cranial window preparations that acidic hypercapnic but not isohydric hypercapnic superfusate caused pial arteriolar dilatation (Wahl et al., 1970;. Acidic hypercapnic but not isohydric hypercapnic solutions also dilated precapillary microvessels in rat cerebral cortex brain slices (Nakahata et al., 2003). Similarly, alkaline hypocapnic but not isohydric hypocapnic superfusate contracted cat and rat pial arterioles in cranial window preparations (Wahl et al., 1970; and pressurized segments of rat penetrating cerebral arterioles (Apkon and Boron, 1995).
Additionally, the magnitude of the dilatation/contraction in response to solutions with altered pH are similar in the absence and presence of corresponding changes in pCO 2 . For example, in a cranial window in the cat, acidic hypercapnic and acidic isocapnic superfusate elicited similar magnitudes of dilatation of pial arterioles . Also, similar magnitudes of dilatation following challenge with acidic hypercapnic and acidic isocapnic solutions were observed in several in vitro vascular preparations from the rat, including basilar and middle cerebral artery rings and pressurized segments of middle cerebral artery and parenchymal vessels (You et al., 1994;Tian et al., 1995;Peng et al., 1998a,b;Dabertrand et al., 2012). Alkaline hypocapnic and alkaline isocapnic solutions also elicited similar magnitudes of contraction of pial arterioles in a cranial window in the cat  and in rings of rabbit basilar artery (Zuccarello et al., 2000a,c).
Also in support of local extracellular pH and not pCO 2 as the major determinant of vascular contractility in response to respiratory hypercapnia/hypocapnia is the ability of alkaline isocapnic superfusate to markedly reduce the increased CBF due to respiratory hypercapnia in rat cerebral cortex . Respiratory hypercapnia-induced increased regional CBF was also greatly decreased following ventriculocisternal infusion of pH 7.52 solution containing 60 mM HCO − 3 in the dog (Koehler and Traystman, 1982). Consistent with the overall premise that local extracellular pH and/or pCO 2 is the major determinant of vascular contractility is the conclusion that alkaline hypocapnic superfusate completely prevented respiratory hypercapniainduced pial vessel dilatation in the cat . On the other hand, after taking into account the decrease in basal pial vessel diameter due to the alkaline hypocapnic superfusate, the ∼27% pial dilatation due to respiratory hypercapnia was only reduced to ∼18% .
Studies in both isolated and in situ vascular preparations suggest that pCO 2 independently of pH can regulate CBF. In isolated ring segments of cat middle cerebral artery, presumably with intact endothelium, significant contraction was induced by lowering pCO 2 in the bathing solution from 37 to 14 mmHg while maintaining pH at 7.4 (Harder and Madden, 1985). Furthermore, although pH 7.6/pCO 2 14 mmHg solution further increased contraction, relaxation was induced upon subsequent elevation of pCO 2 to 37 mmHg while pH was maintained at 7.6 (Harder and Madden, 1985).
Also, while acidic hypercapnic solution dilated endothelium intact helical strips of dog basilar and middle cerebral artery contracted with 20 mM KCl, return to normal pH through elevation of HCO − 3 to 75 mM in the continued presence of elevated pCO 2 resulted in initial re-constriction to control levels followed by partial relaxation (Toda et al., 1989). These findings (Toda et al., 1989) suggest that elevated pCO 2 levels in the absence of a change in pH can cause relaxation.
In other vascular preparations, elevated pH appears insufficient to elicit contraction (Edvinsson and Sercombe, 1976;Aoyama et al., 1999;Kim et al., 2004). Specifically, in rabbit basilar artery ring/strip preparations elevation of extracellular pH to 7.9 with unchanged CO 2 did not cause contraction (Aoyama et al., 1999;Kim et al., 2004). Whether the lack of contraction in response to elevated pH was the result of nitric oxide synthase (NOS) inhibition by N ω -nitro-L-arginine (L-NNA; Kim et al., 2004) or absence of the endothelium (Aoyama et al., 1999) should be considered. On the other hand, in cat middle cerebral artery rings presumably with intact endothelium, elevation of extracellular pH only occasionally induced contraction and, even then, the contraction was of small magnitude (Edvinsson and Sercombe, 1976).
There is also some evidence from our laboratory which suggests that pH as well as pCO 2 in the CSF compartment do not serve as the major regulators of respiratory hypocapnia-induced vasoconstriction (Yoon et al., 2002b). Using a cranial window preparation in the rabbit (with underlying ketamine/xylazineinduced acute metabolic alkalosis) we demonstrated that the magnitude of contraction of the basilar artery to lowered pCO 2 as the result of increased respiration was similar with superfusates at pH 7.3/49.0 mmHg pCO 2 (gassed with 8% CO 2 ) and pH 7.5/30.6 mmHg pCO 2 (gassed with 5% CO 2 ; Yoon et al., 2002b).
Additionally, we compared laser-Doppler CBF in rat ventral midbrain in response to respiratory hypocapnia in a cranial window preparation superfused with pH 7.4 Krebs-Ringer bicarbonate solution and in a preparation which contained only a burr hole in the cranium. Thus, in the superfused preparation the extracellular pH/pCO 2 were constant while in the burr hole preparation the CSF pCO 2 was allowed to decrease, thereby elevating pH. This experimental protocol retains the advantage that the HCO − 3 concentration in the superfusate is maintained at a physiologic level, thereby eliminating possible artifact due to elevated HCO − 3 concentration (Yoon et al., 2003; see "Physiologic and nonphysiologic parameters"). Increased respiration, which lowered arterial pCO 2 levels to similar magnitudes in both the superfused and burr hole preparations (from ∼47 mmHg to ∼22 mmHg), induced similar magnitudes of decreased CBF (Figure 1). Thus, the combined observations of the apparent lack of effect of extracellular (CSF) pH/pCO 2 on the contraction/decreased CBF due to lowered pCO 2 by increased respiration (Yoon et al., 2002b; Figure 1), along with the lack of effect of metabolic alkalosis on CBF (Nau et al., 1999;Anderson and Meyer, 2002), may suggest that pCO 2 also regulates cerebrovascular contractility through an action within the arterial blood compartment.
It is of interest to speculate that this regulation could occur through an action of arterial pCO 2 on the endothelium. Indeed, a possible mechanism underlying such a regulatory pathway may reflect the following: (1) increased pCO 2 , but not lowered extracellular pH, decreased intracellular pH and elevated intracellular Ca 2+ in cultured rat aorta endothelial cells (Ziegelstein et al., 1993) and (2) inhibition of endothelial SK Ca /IK Ca channels resulted in contraction of pressurized parenchymal vessels in rat and also reduced laser-Doppler CBF in mice (Hannah et al., 2011). Thus, the increased/decreased endothelial intracellular Ca 2+ levels could regulate smooth muscle contractility through changes in smooth muscle membrane potential as determined by negative charge transfer via myo-endothelial FIGURE 1 | Effects of respiratory hypocapnia in the presence and absence of superfusate and of NO synthase inhibitor on blood flow velocity in rat ventral midbrain. Respiratory rate and/or tidal volume were increased in cranial window preparations superfused with Krebs-Ringer bicarbonate solution (−Dura) or in preparations with a burr hole in the cranium (+Dura). Laser-Doppler flow (flow velocity) was calculated as percent baseline (top panel). Arterial blood gas parameters and mean arterial pressure (MAP) are indicated in the lower panels and were similar in preparations with and without dura. Additional superfused preparations were exposed to 0.1 mM L-NNA for 30 min prior to respiratory hypocapnic challenge. gap junctions and/or release of K + (Sandow et al., 2009;Edwards et al., 2010). It is also of interest to note that the pathway responsible for the release of prostaglandin I 2 and H 2 S, which were suggested to mediate respiratory hypercapniainduced dilatation of pial vessels in the newborn pig, was schematically illustrated to result from the lowering of intracellular pH in endothelial cells by elevated CO 2 (Leffler et al., 2011). Thus, as illustrated in Figure 2, CO 2 modulation of EDHF release as well as endothelial release of prostaglandin I 2 and H 2 S may represent pathways for the regulation of vascular contractility.
Clearly, an endothelial action of pCO 2 to regulate contractility through these and possibly other endothelial derived vasoactive factors (see below) necessitates the presence of a functional endothelium. Consistent with this requirement is the demonstration that localized light-dye endothelial injury abolished respiratory hypercapnia-induced dilatation in pial arterioles of newborn and juvenile pigs (Willis and Leffler, 2001). On the other hand, respiratory hypercapnia-induced dilatation of rat pial arterioles was not inhibited following localized light-dye injury of the endothelium . Whether these contrasting findings of endothelial dependency Willis and Leffler, 2001) is related to different animal age and/or species remains a possibility. FIGURE 2 | Potential cellular sites of action and vasoactive factor involvement in respiratory hypercapnia/hypocapnia-mediated relaxation/contraction. The effects of respiratory hypercapnia/hypocapnia may be mediated through accompanying changes in pH and/or directly by pCO 2 . pH and/or pCO 2 act on the smooth muscle as well as on the endothelium, nerves, and astrocytes. These latter cell types release vasoactive factors, including PGI 2 , H 2 S, NO, endothelium-derived hyperpolarizing factor (EDHF), and endothelin-1 (ET-1).

NO
Direct evidence for CO 2 modulation of NO release was recently demonstrated by isohydric hypercapnic-and isohydric hypocapnic solution-induced increased and decreased NO release, respectively, from both cultured endothelium from adult human temporal lobe microvessels and astrocytes from human fetus (Fathi et al., 2011). Also consistent with the pCO 2 rather than pH regulation of NO release is the demonstration that a neuronal NOS inhibitor partially prevented respiratory hypercapniainduced dilatation of rat pial arterioles while dilatation due to acidic isocapnic superfusate remained unaltered (Xu et al., 2004). Thus, as illustrated in Figure 2, CO 2 modulation of NO release from the endothelium, astrocytes, and neurons may represent additional pathways for the regulation of vascular contractility. The relevance of the decreased NO release due to lowered pCO 2 (Fathi et al., 2011), however, is not readily apparent since the time course for the decreased release was delayed as compared to the rapid inhibitory effect of systemic NOS inhibitor on the respiratory hypercapnia-elevated cortical CBF in, e.g., primates (Thompson et al., 1996). Thus, it was suggested that the modulation of NO release in response to pCO 2 is related to non-acute changes in contractility (Fathi et al., 2011). It is also of interest to speculate that CO 2 may modulate NO release from cellular sites other than endothelium and astrocytes, such as neurons (Xu et al., 2004;reviewed in Toda et al., 2009).
The relationship between the decreased NO release by isohydric hypocapnic solution (Fathi et al., 2011) and respiratory hypocapnia-induced cerebral vasoconstriction is also not clear since NOS inhibitors did not reduce the contraction elicited by respiration-induced lowered pCO 2 in the rabbit (Toda et al., 1989) and rat basilar artery in situ (21.8% and 22.3% contraction in the absence and presence of L-NNA, respectively; SH Yoon, M Zuccarello, RM Rapoport, unpublished observation). L-NNA Also did not reduce the decreased laser-Doppler flow in rat ventral midbrain due to lowered pCO 2 (Figure 1), even though L-NNA contracted the rat basilar artery in situ by ∼10% (SH Yoon, M Zuccarello, RM Rapoport, unpublished observation).
Although L-NNA did not decrease basal laser-Doppler flow in rat ventral midbrain (Figure 1), this lack of effect could reflect regional differences, as previously observed following systemic administration of NOS inhibitor in the cat (Kovách et al., 1992). Indeed, L-NNA superfusion decreased basal laser-Doppler CBF in rat cerebral cortex (Iadecola and Xu, 1994;Iadecola and Zhang, 1996), while not in cerebellum (Fabricius and Lauritzen, 1994). However, in possible contrast to the decreased basal flow in rat cerebral cortex (Iadecola and Xu, 1994;Iadecola and Zhang, 1996), basal CBF was not decreased in rat parietal cortex (Fabricius and Lauritzen, 1994).

ENDOTHELIN-1
Evidence for a role of endothelin (ET)-1 in pCO 2 -or, for that matter, pH-mediated changes in contractility is sparse. We demonstrated in rabbit basilar artery in situ that contraction of the rabbit basilar artery to lowered pCO 2 by increased respiration as well as to isocapnic alkaline superfusate was relaxed by ET receptor antagonists (Yoon et al., 2002a). Additionally, pretreatment with an ET converting enzyme inhibitor decreased the contraction of the rabbit basilar artery to lowered pCO 2 by 50%, although challenge with the ET converting enzyme inhibitor during the plateau contraction resulted in only 20% relaxation, while relaxing the contraction to isocapnic alkaline superfusate by 75% (Yoon et al., 2002a). We also demonstrated that ET receptor antagonists relaxed contraction to hypocapnic-and isocapnicalkaline solution in rabbit basilar artery in vitro (Zuccarello et al., 2000a,b,c).
Although, elevated levels of arterial ET-1 were not observed in humans following hyperventilation for 4 min or for repeated 2 min intervals (Jordan et al., 2002;Peebles et al., 2008), alkaline hypocapnic solution increased ET-1 release from pig cerebral endothelial cells in culture (Yoshimoto et al., 1991). However, since the effect of isohydric hypocapnic solution on ET-1 release was not investigated, it remains unclear whether the alkaline pH and/or lowered pCO 2 were responsible for the increased ET-1 release (Yoshimoto et al., 1991). Additionally, based on the ability of lowered pCO 2 to decrease NO release (Fathi et al., 2011), it may be considered that respiratory hypocapnia-induced increased ET-1 release is secondary to disinhibition of decreased ET-1 release by NO (Boulanger and Luscher, 1990). On the other hand, this ET-1 release mechanism would not be present in the rabbit basilar artery since L-NNA did not inhibit contraction to respiration-induced lowered pCO 2 (Yoon et al., 2002b). Pathways which depict the pCO 2 /pH regulation of ET-1 release are also represented in Figure 2.

20-HYDROXYICOSATETRAENOIC ACID
The role of 20-hydroxyicosatetraenoic acid (20-HETE) in the CO 2 /pH regulation of contractility was investigated in pial arterioles of fetal sheep in situ (Ohata et al., 2010). Superfusion with the omega-hydroxylase inhibitor, 17-octadecynoic acid, did not prevent contraction of the pial vessels to respiratory hypocapnia (Ohata et al., 2010). Thus, it does not appear, at least based on this study (Ohata et al., 2010), that 20-HETE is involved in the CO 2 /pH regulation of cerebral vascular contractility.

SIGNALING PATHWAYS IN SMOOTH MUSCLE
An action of CO 2 independent of or concomitant with that of pH was also demonstrated by the greater increase in inositol trisphosphate levels by alkaline hypocapnic than alkaline isocapnic solution in cultured cerebral microvascular smooth muscle cells from piglets (Albuquerque et al., 1995). Additionally, in rat middle cerebral artery rings and pressurized segments, while both acidic hypercapnic and acidic isocapnic solutions induced relaxation, these solutions elicited membrane hyperpolarization and depolarization, respectively (Peng et al., 1998a,b). Also, acidic isocapnic and essentially isohydric hypercapnic solution were without effect and hyperpolarized rat middle cerebral artery muscle cells, respectively (Harder, 1982).

PHYSIOLOGIC AND NON-PHYSIOLOGIC PARAMETERS
Some of the varied effects of pCO 2 and pH on cerebrovascular contractility described above could be attributed to vessel location within the cerebrovascular tree as well as animal age and species. This possibility was previously described with respect to the involvement of K ATP channels in the pH-mediated relaxation/ contraction of cerebral vessels (Rosenblum, 2003). In addition, experimental parameters or inherent differences between preparation types may also result in ambiguity with regards to pCO 2and pH-dependent mechanisms, as follows: (1) Non-physiologic pH: Hypercapnia and hypocapnia can lower and elevate CSF pH by ∼0.3 pH units, i.e., to ∼7.1 and ∼7.6, respectively (Plum and Posner, 1967;Adaro et al., 1969;Nilsson and Busto, 1973). Thus, non-physiologic mechanisms may be elicited by solutions with pH values outside of this range. For example, reversible contraction was elicited by pH 7.8 but not pH 8.0 Hepes buffered solution in isolated perfused penetrating arterioles of the rat middle cerebral artery (Apkon and Boron, 1995).
(2) Absence of HCO − 3 : The lack of inclusion of HCO − 3 may produce artifacts since HCO − 3 , through the Na + dependent Cl − -HCO − 3 exchanger, is required to recover from an alkali load and for normal cell homeostasis (Kikeri et al., 1990;Neylon et al., 1990;Batlle et al., 1993). The importance of HCO − 3 was also shown by the amplification of changes in intracellular pH when intraluminally pressurized rat cerebral arterioles were exposed to alkaline hypocapnic solution as compared to alkalinized Hepes solution (Apkon and Boron, 1995). Moreover, we demonstrated that alkaline Tris buffered solution but not alkaline hypocapnic Krebs-Ringer bicarbonate solution activated extracellular regulated protein kinase in brain microvascular endothelial cells (Motz et al., 2006).
(3) Alkaline isocapnic solution: A standard procedure for the evaluation of the effects of alkaline pH in the absence of changes in CO 2 is through the use of alkaline isocapnic solution, attained through the elevation of the HCO − 3 concentration. However, we observed that even relatively modest elevation of the HCO − 3 concentration from 25 mM to 35 mM or to 50 mM, yielding alkaline isocapnic superfusate with pH elevated from 7.4 to 7.6 and to 7.7, respectively, desensitized the contractile response of the rabbit basilar artery in situ to subsequent challenge with these alkaline isocapnic superfusates and to respiratory hypocapnia (Yoon et al., 2003). It should be noted, however, that the findings of desensitization (Yoon et al., 2003) may be complicated by the underlying anesthetic (ketamine/xylazine)-induced acute metabolic alkalosis (Yoon et al., 2002b). Moreover, unexpectedly, alkaline hypocapnic superfusate failed to contract the rabbit basilar artery (Yoon et al., 2002a). Despite these potential complications, the desensitization of contraction to alkaline isocapnic superfusate and respiratory hypocapnia by prior alkaline isocapnic superfusate may suggest that elevated HCO − 3 concentration elicits additional cellular events not present during acute changes in pCO 2 . Indeed, the calculated HCO − 3 concentration in CSF after 1-2 h of respiratory hypercapnia and hypocapnia was not significantly altered in human and only small changes were observed in dog and cat (Paddle and Semple, 1969 and references therein;Shibata et al., 1976;Kazemi and Javaheri, 1978). For example, in dog even after 2 h of respiratory hypercapnia, which increased CSF pCO 2 from 46.7 to 80 mmHg, the calculated HCO − 3 concentration increased by only 3.3 mM (Shibata et al., 1976). Also in dog, even after 2 h of respiratory hypocapnia, which decreased CSF pCO 2 from 48 to 30 mmHg, the calculated HCO − 3 concentration decreased by only 4.9 mM (Kazemi and Javaheri, 1978).
(4) Levels of pCO 2 /pH: Different mechanisms of dilatation/contraction may be evoked depending on the level of pCO 2 /pH. For example, the K ATP channel blocker, glyburide, inhibited dilatation to moderate but not severe hypercapnia in arterioles of the rabbit cerebral cortex (Faraci et al., 1994). Also, NOS inhibition decreased laser-Doppler CBF to moderate and severe but not to extreme hypercapnia in rat cerebral cortex (Iadecola and Zhang, 1994). Possibly consistent with the suggestion that the contraction due to respiratory hypocapnia involves multiple mechanisms is our demonstration in rabbit basilar artery in situ that alkaline isocapnic superfusate with elevated HCO − 3 concentration only partially inhibited subsequent contraction to respiratoryinduced lowered pCO 2 (Yoon et al., 2003). Moreover, the magnitude of decreased contraction to respiratory-induced lowered pCO 2 was similar to the decreased contraction to a second challenge with isocapnic alkaline superfusate (Yoon et al., 2003). Thus, the contraction due to respirationinduced lowered pCO 2 may be composed of components sensitive and insensitive to desensitization by alkaline isocapnic solution (Yoon et al., 2003). It should also be considered that the mechanism underlying contraction to pCO 2 /pH may differ depending on the time post initial alteration in pCO 2 /pH. (5) In vitro vs. in vivo: We demonstrated in rabbit basilar artery rings in vitro that maintained contraction to alkaline hypocapnic and isocapnic solutions was infrequently initiated in the absence of initial triggering with repeated challenge with KCl, serotonin or NOS inhibitor (Zuccarello et al., 2000a,b,c). Possibly consistent with these findings (Zuccarello et al., 2000a,b,c) are observations in rabbit basilar and cat middle cerebral artery ring/strip preparations in which elevation of extracellular pH did not cause or elicited only minimal contraction (Edvinsson and Sercombe, 1976;Aoyama et al., 1999;Kim et al., 2004). Additionally, contraction of cat middle cerebral artery rings to pH 7.4/pCO 2 14 mmHg solution was highly variable and, in some cases, minimal (Harder and Madden, 1985). Thus, factors may be absent/altered in in vitro ring/strip preparations that are present in vivo. It is of interest to speculate that intraluminal pressure is a factor which enhances alkaline hypocapnic solution-induced contraction in vivo and is absent from in vitro ring/strip preparations. Indeed, smooth muscle depolarization associated with intraluminal pressure (Dunn et al., 1994) could influence the actions of vasoactive factors as well as direct effects of CO 2 /pH on the smooth muscle. Additionally, with respect to contractile/relaxant mechanism, the absence of intraluminal pressure can completely change the mechanism whereby endothelium-derived factors elicit changes in contractility. For example, in small arteries of mouse gracilis muscle the absence of intraluminal pressure abolished the ability of acetylcholine to induce relaxation through www.frontiersin.org September 2012 | Volume 3 | Article 365 | 5 endothelium-derived hyperpolarizing factor (Boettcher and de Wit, 2011). Also, differing basal neuronal and endothelial NO release in in vivo vs. in vitro preparations may influence findings with respect to NO-dependency (Toda et al., 1996). (6) Absence of cell types: Depending on the vessel type/location, findings in isolated vessels could be altered by the absence of astrocytes and lack of nerve input since these cells have been implicated in changes in contractility in response to altered CO 2 (Xu et al., 2004;Toda et al., 2009;Fathi et al., 2011). For example, damage of the glial limitans with L-α-aminoadipic acid resulted in partial inhibition of respiratory hypercapniainduced dilatation of rat pial arterioles while not altering dilatation to acidic isocapnic superfusate (Xu et al., 2004). Thus, these findings suggest the involvement of glial and neuronal cells in pCO 2 regulation of CBF (Xu et al., 2004). This involvement appears to be age and possibly species dependent since L-α-aminoadipic acid failed to inhibit respiratory hypercapnia-induced dilatation of pial arterioles in newborn pig (Parfenova et al., 2012).

CONCLUSION
pCO 2 and pH may independently and concomitantly regulate cerebrovascular contractility. While regulation by pH appears restricted to the CSF, regulation by pCO 2 may occur through an action within the CSF and also possibly within the arterial blood. This latter regulation could occur through endotheliumdependent mechanisms. Additional sites of pCO 2 action, as well of pH, may include nerves and astrocytes. The possible effects of pCO 2 and pH on these different cell types and the involvement of vasoactive factors are outlined in Figure 2.
The underlying pCO 2 /pH regulatory mechanism(s) also likely varies with vessel type and magnitude and duration of challenge with altered pCO 2 /pH. Furthermore, these mechanisms may reflect redundant pathways (Irikura et al., 1995;Wang et al., 1998;Xu et al., 2004;Fathi et al., 2011;Leffler et al., 2011). Finally, with respect to experimental variables, the preparation and conditions employed likely influence the observed mechanism. Overall, the numerous and interactive regulatory pathways provides precise, regional regulation of CBF by pCO 2 and pH (Ito et al., 2000;Willie et al., 2012).