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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Physiol. | doi: 10.3389/fphys.2019.01241

Anti-Inflammatory and Regulatory Effects of Huanglian Jiedu Decoction on Lipid Homeostasis and the TLR4/MyD88 Signaling Pathway in LPS-Induced Zebrafish

 Junyi Zhou1, Xinru Gu1, Xiaorui Fan1, Yanyan Zhou1, Hongjie Wang1, Nan Si1, Jian Yang1, Baolin Bian1* and Haiyu Zhao1*
  • 1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, China

Huanglian Jiedu Decoction (HLJDD) has been used in the clinical treatment of inflammatory conditions. To clarify the mechanism of its comprehensive anti-inflammatory activities, the correlation between lipid homeostasis and the TLR4/MyD88 signaling pathway in zebrafish was established in the present study. In the lipopolysaccharide (LPS)-induced inflammation in zebrafish model, RT-PCR assays of five inflammatory cytokines and six targeted proteins were measured. Lipidomics analysis was conducted to identify potential lipid markers. HLJDD displayed strong efficacies, with a 61% anti-inflammatory rate at a concentration of 50 µg/mL. The activation of TLR4/MyD88 played an essential role in the inflammatory process. All protein indexes in the HLJDD group exhibited a tendency to reverse back to normal levels. Moreover, 79 potential pathological lipid biomarkers were identified. Compared with the model group, 61 therapeutic lipid biomarkers were detected in HLJDD group. Most perturbations of lipids were ameliorated by HLJDD, mainly through the glycerophospholipid metabolic pathway. In the visual network study, the corresponding lipoproteins such as PLA2, SGMS and SMDP were observed as important intermediates between lipid homeostasis and the TLR4/MyD88 signaling pathway.

Keywords: Huanglian Jiedu Decoction, Anti-inflammatory activities, lipidomic profiles, TLR4/MyD88 signaling pathway, LPS (lipopolysaccharide), Zebrafish

Received: 02 May 2019; Accepted: 10 Sep 2019.

Copyright: © 2019 Zhou, Gu, Fan, Zhou, Wang, Si, Yang, Bian and Zhao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Prof. Baolin Bian, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China, blbian@icmm.ac.cn
Prof. Haiyu Zhao, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China, hyzhao@icmm.ac.cn