AUTHOR=Kartaram Shirley W. , van Norren Klaske , Schoen Eric , Teunis Marc , Mensink Marco , Verschuren Martie , M’Rabet Laura , Besseling-van der Vaart Isolde , Mohrmann Karin , Wittink Harriet , Garssen Johan , Witkamp Renger , Pieters Raymond 

TITLE=Kinetics of Physiological Responses as a Measure of Intensity and Hydration Status During Experimental Physical Stress in Human Volunteers

JOURNAL=Frontiers in Physiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2020.01006

DOI=10.3389/fphys.2020.01006

ISSN=1664-042X

ABSTRACT=<sec><title>Introduction</title><p>Strenuous physical stress induces a range of physiological responses, the extent depending, among others, on the nature and severity of the exercise, a person’s training level and overall physical resilience. This principle can also be used in an experimental set-up by measuring time-dependent changes in biomarkers for physiological processes. In a previous report, we described the effects of workload delivered on a bicycle ergometer on intestinal functionality. As a follow-up, we here describe an analysis of the kinetics of various other biomarkers.</p></sec><sec><title>Aim</title><p>To analyse the time-dependent changes of 34 markers for different metabolic and immunological processes, comparing four different exercise protocols and a rest protocol.</p></sec><sec><title>Methods</title><p>After determining individual maximum workloads, 15 healthy male participants (20–35 years) started with a rest protocol and subsequently performed (in a cross-over design with 1-week wash-out) four exercise protocols of 1-h duration at different intensities: 70% W<sub><italic>max</italic></sub> in a hydrated and a mildly dehydrated state, 50% W<sub><italic>max</italic></sub> and intermittent 85/55% W<sub><italic>max</italic></sub> in blocks of 2 min. Perceived exertion was monitored using the Borg’ Rating of Perceived Exertion scale. Blood samples were collected both before and during exercise, and at various timepoints up to 24 h afterward. Data was analyzed using a multilevel mixed linear model with multiple test correction.</p></sec><sec><title>Results</title><p>Kinetic changes of various biomarkers were exercise-intensity-dependent. Biomarkers included parameters indicative of metabolic activity (e.g., creatinine, bicarbonate), immunological and hematological functionality (e.g., leukocytes, hemoglobin) and intestinal physiology (citrulline, intestinal fatty acid-binding protein, and zonulin). In general, responses to high intensity exercise of 70% W<sub><italic>max</italic></sub> and intermittent exercise i.e., 55/85% W<sub><italic>max</italic></sub> were more pronounced compared to exercise at 50% W<sub><italic>max</italic></sub>.</p></sec><sec><title>Conclusion</title><p>High (70 and 55/85% W<sub><italic>max</italic></sub>) and moderate (50% W<sub><italic>max</italic></sub>) intensity exercise in a bicycle ergometer test produce different time-dependent changes in a broad range of parameters indicative of metabolic activity, immunological and hematological functionality and intestinal physiology. These parameters may be considered biomarkers of homeostatic resilience. Mild dehydration intensifies these time-related changes. Moderate intensity exercise of 50% W<sub><italic>max</italic></sub> shows sufficient physiological and immunological responses and can be employed to test the health condition of less fit individuals.</p></sec>