TY - JOUR AU - Uzelac, Ilija AU - Crowley, Christopher J. AU - Iravanian, Shahriar AU - Kim, Tae Yun AU - Cho, Hee Cheol AU - Fenton, Flavio H. PY - 2022 M3 - Original Research TI - Methodology for Cross-Talk Elimination in Simultaneous Voltage and Calcium Optical Mapping Measurements With Semasbestic Wavelengths JO - Frontiers in Physiology UR - https://www.frontiersin.org/articles/10.3389/fphys.2022.812968 VL - 13 SN - 1664-042X N2 - Most cardiac arrhythmias at the whole heart level result from alteration of cell membrane ionic channels and intracellular calcium concentration ([Ca2+]i) cycling with emerging spatiotemporal behavior through tissue-level coupling. For example, dynamically induced spatial dispersion of action potential duration, QT prolongation, and alternans are clinical markers for arrhythmia susceptibility in regular and heart-failure patients that originate due to changes of the transmembrane voltage (Vm) and [Ca2+]i. We present an optical-mapping methodology that permits simultaneous measurements of the Vm - [Ca2+]i signals using a single-camera without cross-talk, allowing quantitative characterization of favorable/adverse cell and tissue dynamical effects occurring from remodeling and/or drugs in heart failure. We demonstrate theoretically and experimentally in six different species the existence of a family of excitation wavelengths, we termed semasbestic, that give no change in signal for one dye, and thus can be used to record signals from another dye, guaranteeing zero cross-talk. ER -