AUTHOR=Seabra Ana R., Carvalho Helena G. TITLE=Glutamine synthetase in Medicago truncatula, unveiling new secrets of a very old enzyme JOURNAL=Frontiers in Plant Science VOLUME=6 YEAR=2015 URL=https://www.frontiersin.org/articles/10.3389/fpls.2015.00578 DOI=10.3389/fpls.2015.00578 ISSN=1664-462X ABSTRACT=Glutamine synthetase (GS) catalyzes the first step at which nitrogen is brought into cellular metabolism and is also involved in the reassimilation of ammonium released by a number of metabolic pathways. Due to its unique position in plant nitrogen metabolism, GS plays essential roles in all aspects of plant development, from germination to senescence, and is a key component of nitrogen use efficiency (NUE) and plant yield. Understanding the mechanisms regulating GS activity is therefore of utmost importance and a great effort has been dedicated to understand how GS is regulated in different plant species. The present review summarizes exciting recent developments concerning the structure and regulation of GS isoenzymes, using the model legume Medicago truncatula. These include the understanding of the structural determinants of both the cytosolic and plastid located isoenzymes, the existence of a seed-specific GS gene unique to M. truncatula and closely related species and the discovery that GS isoenzymes are regulated by nitric oxide at the post-translational level. The data is discussed and integrated with the potential roles of the distinct GS isoenzymes within the whole plant context.