Rhizospheric Bacterial Strain Brevibacterium casei MH8a Colonizes Plant Tissues and Enhances Cd, Zn, Cu Phytoextraction by White Mustard

Environmental pollution by heavy metals has become a serious problem in the world. Phytoextraction, which is one of the plant-based technologies, has attracted the most attention for the bioremediation of soils polluted with these contaminants. The aim of this study was to determine whether the multiple-tolerant bacterium, Brevibacterium casei MH8a isolated from the heavy metal-contaminated rhizosphere soil of Sinapis alba L., is able to promote plant growth and enhance Cd, Zn, and Cu uptake by white mustard under laboratory conditions. Additionally, the ability of the rifampicin-resistant spontaneous mutant of MH8a to colonize plant tissues and its mechanisms of plant growth promotion were also examined. In order to assess the ecological consequences of bioaugmentation on autochthonous bacteria, the phospholipid fatty acid (PLFA) analysis was used. The MH8a strain exhibited the ability to produce ammonia, 1-amino-cyclopropane-1-carboxylic acid deaminase, indole 3-acetic acid and HCN but was not able to solubilize inorganic phosphate and produce siderophores. Introduction of MH8a into soil significantly increased S. alba biomass and the accumulation of Cd (208%), Zn (86%), and Cu (39%) in plant shoots in comparison with those grown in non-inoculated soil. Introduced into the soil, MH8a was able to enter the plant and was found in the roots and leaves of inoculated plants thus indicating its endophytic features. PLFA analysis revealed that the MH8a that was introduced into soil had a temporary influence on the structure of the autochthonous bacterial communities. The plant growth-promoting features of the MH8a strain and its ability to enhance the metal uptake by white mustard and its long-term survival in soil as well as its temporary impact on autochthonous microorganisms make the strain a suitable candidate for the promotion of plant growth and the efficiency of phytoextraction.


INTRODUCTION
The continued industrialization of countries has led to extensive environmental problems that induce the contamination of soil and water (Rajkumar and Freitas, 2008). Among the pollutants, heavy metals pose a critical concern to human health and the environment (Rajkumar et al., 2009a). Due to the widespread contamination, searching for innovative ways to remove metals from the environment has become a priority in the remediation field (Rajkumar et al., 2009b;Ali et al., 2013). One of the most promising strategies is phytoextraction, which is defined as the use of plants to take up pollutants from contaminated soil (Salt et al., 1998;Glick, 2010;Płociniczak et al., 2013b). The success of metal extraction depends on many factors, but the most important are a plant's ability to uptake and translocate metals to its stems and leaves, metal bioavailability and soil type (Glick, 2010;De-Bashan et al., 2012;Płociniczak et al., 2013a). The results of many studies have shown that the efficiency of heavy metal phytoextraction may be supported by metalresistant bacteria that belong to the plant growth-promoting bacteria group (PGPB; Glick, 2005;Jing et al., 2007;Li and Ramakrishna, 2011;Li et al., 2012;Sun et al., 2014). PGPB include both rhizospheric (PGPR) and endophytic (PGPE) bacteria (Ma et al., 2011a). Because of the extensive root exudation of many easily degradable compounds, the rhizosphere is a nutrient-rich environment for beneficial bacteria that may colonize the internal tissues of plants and exist as endophytes. Although bacterial plant growth-promoting endophytes (PGPEs) exist in plants to varying degrees, and despite the fact that they are transient, they are often capable of triggering physiological changes that promote the growth and development of plants, even those that are growing in metal-contaminated soil (Rajkumar et al., 2009a).
Generally, the beneficial effects of endophytes are greater than those of many rhizobacteria and these might be enhanced when a plant is growing under either biotic or abiotic stress conditions. It has been shown that PGPE may confer plants with a higher tolerance to heavy metal stress and may stimulate the growth of the host plant through several mechanisms (Ma et al., 2011b). That is one reason why PGPE apart from their application as biocontrol agents and biofertilizers are used to enhance in situ phytoextraction (Chen et al., 2010;Becerra-Castro et al., 2011). Heavy-metal-resistant endophytes can enhance plant growth, decrease metal phytotoxicity and affect metal translocation and accumulation in plants and thus play a significant role in the adaptation of plants to a polluted environment (Sessitsch et al., 2013). The inoculation of plants with PGPR/PGPE has been shown to enhance the growth of plants and their development in heavy metals and/or soils contaminated with organic pollutants (Becerra-Castro et al., 2011;Thavasi et al., 2014). Generally, PGPB interact directly or indirectly with a host plant through several mechanisms. These mechanisms involve the production of indole acetic acid (IAA), phytohormones, 1-aminocyclopropane-1-carboxylic acid deaminase (ACCD) and biosurfactants (Arshad et al., 2007;Gravel et al., 2007;Juwarkar et al., 2008;Shoebitz et al., 2009;Li et al., 2012;Sun et al., 2014;Thavasi et al., 2014). Moreover, metal-resistant microorganisms have been shown to increase the availability of heavy metals in soil through soil acidification by producing siderophores and/or by mobilizing metal phosphates (Abou-Shanab et al., 2008;Jing et al., 2012;Płociniczak et al., 2013b).
The aim of this study was to estimate the potential of a metal-resistant strain of Brevibacterium casei MH8a to enhance Zn, Cd, and Cu uptake by white mustard under laboratory conditions. Moreover, its potential to promote the growth of plants was also determined. Additionally, the ability of MH8a to colonize the internal tissues of Sinapis alba and the ecological consequences of its introduction into soil on autochthonous bacterial communities were also determined.

Isolation and Identification of Metal-Resistant Bacteria
The B. casei MH8a strain was chosen from 12 strains isolated from metal-contaminated rhizosphere soil of S. alba L. collected around a non-ferrous steelworks in Dąbrowa Górnicza, Upper Silesia, Poland, which contained the following concentrations of heavy metals: Zn 926, Cd 32, Cu 60, and Ni 150 mg kg −1 dry weight (dw). The metal-resistant bacteria were isolated on a 10% Tryptic Soy Agar (TSA) medium that was supplemented with 5 mM of zinc as ZnCl 2 . The plates were incubated at 28 • C for 7 days. Isolated strains were identified using the MIDI microbial identification system (MIDI, Newark, DE, USA) according to the producer's procedure. Identification of MH8a strain was also confirmed based on 16S rRNA gene sequence analysis. For 16S rRNA gene amplification, the universal bacterial primers 8F (5 AGTTTGATCATCGCTC AG 3 ) and 1492R (5 GGTTACCTTGTTACGACTT 3 ) targeting fragment size 1484 bp were used (Pacwa-Płociniczak et al., 2014). The obtained sequence was compared to known 16S rRNA gene sequences using the BLAST server at the National Center for Biotechnology Information (NCBI; https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE= BlastSearch).
DNA sequences were aligned using ClustalW. Phylogenetic analyses were performed by the neighbor-joining (NJ) method to test the support for the phylogeny with a bootstrap analysis based on 1,000 replicates using MEGA software ver. 7.0.

Determination of Metal Minimal Inhibitory Concentration (MIC)
To assess the level of the resistance to heavy metals of the tested strain, the minimal inhibitory concentration (MIC) of Zn, Cu, and Cd was estimated. The MIC values were determined in triplicate in an MES buffered minimal medium (MBMM) as described by Rathnayake et al. (2013). The MBMM medium was supplemented with an increasing content of the metals (1-10 mM of Zn and Cu and 0.5-3 mM of Cd). The MIC was defined as the lowest metal concentration at which bacterial growth was not observed.

Evaluation of Plant Growth-Promoting Activities
Secretion of siderophores by the tested strain was detected using the method described by Schwyn and Neilands (1987) using blue agar plates that contained Chrome azurol S dye (CAS). Orange zones around the colonies on blue agar were considered to be a positive reaction that indicated the production of siderophores.
1-Aminocyclopropane-1-carboxylic acid deaminase activity was assayed according to a modified Honma and Shimomura (1978) method as described by Saleh and Glick (2001). ACCD activity was expressed in nmol of α-ketobutyrate mg −1 h −1 . The protein concentration of microbial cell suspensions was determined using the Bradford (1976) method.
Indole acetic acid production was assessed using Salkowski's reagent according to the modified method of Bric et al. (1991). The IAA concentration in cultures was calculated using the calibration curve of pure IAA (1-100 μg mL −1 of medium) as the standard.
The phosphate solubilizing ability of the tested bacteria was determined on a National Botanical Research Institute's phosphate (NBRIP) agar medium as described by Płociniczak et al. (2013a).
The production of hydrogen cyanide by MH8a was tested using the Lorck (1948) method as shown in Pawlik and Piotrowska-Seget (2015).
The bacterial isolate was tested for the production of ammonia in peptone water according to Cappuccino and Sherman (1992).

Selection of a Rifampicin-Resistant Mutant of MH8a
A spontaneous mutant of the MH8a was selected by plating on a Luria Bertani (LB) medium that was amended with 5 μg mL −1 of rifampicin. Next, the growing colony was reinoculated on LB plates with a successively higher content of rifampicin (up to 150 μg mL −1 of medium). The stability of rifampicin resistance was confirmed by subculturing MH8a rf five times on an LB medium without antibiotic selection. The cultures were stored in 20% glycerol at −80 • C (Płociniczak et al., 2013b). MH8a rf had the same biochemical features as the parental MH8a and was used in pots experiment.

Inoculum Preparation
For soil inoculation, the metal-resistant MH8a rf strain was cultured on a Luria-Bertani (LB) medium on an orbital shaker at 120 rpm (28 • C) for 24 h. The number of bacteria in the inoculum was established based on the turbidimetry and plating methods. The appropriate volume of bacterial culture was centrifuged (6000 rpm, 21 • C, 20 min); the harvested bacteria were washed twice with sterile distilled water and resuspended in 50 mL sterile water.
The experiment had a completely randomized block design with three replications that had two treatments (I) plants growing in soil inoculated with the tested MH8a rf strain and (II) control -plants growing in soil inoculated with distilled water that contained thermal inactivated MH8a rf cells instead of the suspension of living bacteria.
Prior to the experiment, the soil was kept for 2 days at room temperature and sieved. Then the soil moisture was adjusted and maintained at 20% which corresponded to 50% of the maximum water holding capacity of the soil. Seeds of S. alba L. cv. Nakielska were placed in pots (total volume of 500 mL) that contained 400 g of the soil and kept in a growth room under controlled light (14-h photoperiod at 15,000 lux; temperature 23/18 • C light/dark). After 2 weeks, 50 mL of the bacterial solution was poured into the soil up to the number of 10 8 cells of the tested strains g −1 dw soil. Plants (five plants per pot) were grown in the conditions described above for 28 days.

Effect of the Bacterial Inoculant on Plant Biomass and Metal Accumulation
After 28 days of incubation, the plants were removed from the pots and shoots (stems and leaves) and roots were weighed separately. Before weighing, the roots were washed carefully using distilled water. The fresh and dw of shoots and roots as well as heavy metal accumulation in the above and underground parts were measured. The metal concentration in the shoots and roots of S. alba was determined in triplicate using atomic absorption spectrometry as described in Płociniczak et al. (2013b). The usefulness of S. alba in phytoextraction was confirmed by estimating the translocation factor (TF) for the tested metals (Yoon et al., 2006).

Effect of the Bacterial Inoculant on the Structure of Bacterial Community
Changes in the biodiversity and community structure of the autochthonous bacterial populations after soil inoculation with the MH8a rf strain were determined in triplicate at 24 h, 7 and 28 days using the phospholipid fatty acid (PLFA).
Phospholipid fatty acids were isolated from 2 g of fresh soil as described by Pennanen et al. (1999). The fatty acid methyl esters were separated using a gas chromatograph (Hewlett-Packard 6890, USA) with an HP-Ultra 2 capillary column (25 m, 0.22 mm ID) and hydrogen as the carrier gas. PLFA compounds were detected using a flame ionization detector (FID) and identified using MIDI Microbial Identification System software (Sherlock TSBA 6 method and TSBA 6 library; MIDI, Inc., Newark, DE, USA).

Survival of MH8a rf in Soil and its Ability to Colonize Plant Tissues
The number of living MH8a rf cells in soil was determined at 24 h, 7 and 28 days after soil inoculation. In order to estimate the colony-forming units (cfu) of bacteria in soil and plant tissues, the dilution-plate method on Luria-Bertani (LB) agar with the addition of 150 μg mL −1 of rifampicin was used. As a control, a suspension of soil inoculated with thermal inactivated MH8a rf cells was plated on LB agar with the addition of an antibiotic.
Plant colonization by MH8a rf was determined 24 h, 7 and 28 days after soil inoculation with the tested strain. Roots, stems and leaves were surface sterilized with 70% ethanol (2 min), 5% sodium hypochlorite (2 min), and 10% hydrogen peroxide (2 min). The samples were rinsed three times in sterile distilled water to remove the disinfectant. The sterilization process was verified by a plating final wash onto a TSA medium. Plates were incubated at 28 • C for 7 days. If no microbial growth was found, the surface-sterilization process was recognized as being successful. The roots, stems, and leaves were macerated separately in 5 mL of 0.9% NaCl using a mortar and pestle and a 100 μL suspension was plated onto a TSA medium that contained rifampicin at a concentration of 150 μg mL −1 of medium and incubated at 28 • C for 7 days (Kukla et al., 2014). At the same time, rifampicin-resistant bacteria were isolated from the control plants grown in soil inoculated with thermal inactivated MH8a rf strain.

Statistical Analysis
Statistical analysis was performed using STATISTICA 10.0 PL software (StatSoft, Tulsa, OK, USA). Analysis of variance (ANOVA) followed by a post hoc Least Significant Difference test were conducted in order to identify any significant effects of the introduced strains on the plant biomass as well as on the accumulation of heavy metals in the shoots and roots of S. alba. Differences to from the control plants with p < 0.01 in the plant inoculation experiments were considered to be significant. For the pot experiments, data were represented as the mean ± standard deviation (SD) of three replicates. For the PLFA experiments principal component analysis (PCA) was used. One-way multivariate analysis of variance (MANOVA) of the PCA-axes values and post hoc LSD tests (p < 0.05) were applied for the statistical testing of the separation of the profiles along each PC. For the PLFA experiment, data were represented as the mean ± standard deviation (SD) of three replicates.

Identification of Isolate and MIC Values of the Tested Strain
Based on the MIDI-FAME method and 16S rRNA gene sequence analysis, the isolated strain was identified as B. casei and designed as MH8a strain. The phylogenetic analysis showed that the 16S rDNA sequence of B. casei MH8a (KT951720.1) had a 98 and 97% identity with strains B. casei DSM 20657 (NR_041996.1) and B. casei NCDO 2048 (NR_119071.1), respectively (Figure 1).
The MIC values for Zn, Cd, and Cu reached values of 7, 1.5, and 6 mM, respectively.

Biochemical Characteristic of MH8a (PGP Traits)
Among the tested mechanisms that were considered to be potentially responsible for the support of plant growth and heavy metal phytoextraction, MH8a was able to produce ammonia, hydrocyanic acid and indole 3-acetic acid at a concentration of 3.44 ± 0.09 μg IAA mL −1 of medium. The MH8a showed the activity of ACC deaminase at a level of 53.6 ± 1.47 nmol α-ketobutyrate mg −1 h −1 . B. casei MH8a was not able to solubilize Ca 3 (PO 4 ) 2 in an NBRIP medium to produce siderophores on a CAS medium.

Effect of Bacteria Inoculation on Plant Biomass and Metal Accumulation
The inoculation of soil with the tested bacterial strain resulted in a significant increase of the biomass of S. alba as compared to the control plants (Figure 2). MH8a enhanced the fresh and dry weight of shoots by 144 and 51%, respectively, and significantly increased (about 33%) the fresh and dry weight of roots as compared to the control plants.
The concentrations of Zn, Cd, and Cu were significantly higher in plants growing in soil inoculated with MH8a in comparison with the control plants ( Table 1). MH8a led to a significant increase in Zn (87%), Cd (207%), and Cu (39%) accumulation in shoot tissues. The tested strain also caused a significant increase in the accumulation of metals in the roots of S. alba, where the Zn, Cd, and Cu accumulation was 85, 87, and 122% higher as compared to the control plants, respectively.
As is shown by the values of TF, the inoculation of soil with MH8a resulted in a markedly higher Cd translocation from root to shoot. The value of TF for Cd in plants growing in soil treated with MH8a reached a value of 0.91, whereas in the control plants, it was significantly lower (0.55).

Effect of the Bacterial Inoculant on the Structure of Bacterial Community
The inoculation of MH8a into the soil caused temporary changes in the PLFA profiles that were obtained from the treated soil as compared to the control soil (Figure 3). All PLFAs were divided into structural classes and among them hydroxylated, cycloprane, and unsaturated fatty acids were considered to be characteristic for Gram-negative bacteria. In turn, the branched fatty acids indicated the presence of Gram-positive bacteria. The inoculation of MH8a into soil caused an increase of branched fatty acids content (4.4%) in PLFA profiles after 24 h. Additionally, a decrease in the percentage of unsaturated fatty acids (3.3%) was also observed. At the same time, the amount of 18:2ω6,9c decreased from 2.5 to 1.9%. Seven days after soil treatment, the percentage of branched fatty acids decreased about 2.6% in the PLFA profiles as compared to samples at 24 h. At the same time, an increase in the percentage of methylated fatty acids was observed. Analysis of the PLFA profiles from 28 days samples (end of the experiment) showed a decrease in the percentage of branched fatty acids and an increase of 18:2ω6,9c as compared with the PLFA profiles from the 24 h and 7 days samples.
Phospholipid fatty acid profiles differed significantly (p < 0.001) along PC1, which explained 46.26% of the total variability between the tested samples (Figure 4). All PLFA profiles extracted from the soil inoculated with the MH8a strain (24 h, 7 and 28 days) differed significantly as compared to the profiles obtained from the control soil. The most pronounced changes in the bacterial community structure were observed 24 h after soil inoculation with MH8a. Profiles at 24 h also differed significantly from those obtained for soil taken 28 days after soil inoculation with the tested strain. Among the tested samples, PLFA profiles from soil taken at the end of experiment (28 days) had the nearest location to the control samples, but the differences were still statistically significant.

Survival of MH8a in Soil and its Ability to Colonize Plant Tissues
A rifampicin-resistant mutant of the MH8a strain was used for the assessment of its survival in soil and its ability to colonize S. alba tissues after soil inoculation. A gradual decrease in cell number in soil was observed during the experimental period (Figure 5). At the first sampling time, the number of MH8a cells decreased three-fold (to 3.6 × 10 7 cfu g −1 dw of soil) as compared to the number of bacteria introduced into the soil (10 8 cfu g −1 dw of soil). On the last sampling day (28 days), the number of MH8a cells in soil was 4 × 10 3 cfu g −1 of dry soil. The plate inoculation with the plant's suspension showed that no rifampicin-resistant bacteria were isolated from plant tissue 24 h after soil treatment with MH8a. Seven and 28 days after the introduction of MH8a into the soil, the number of bacteria that were isolated from the leaves of S. alba reached a value of 4.9 × 10 3 and 3.6 × 10 3 cfu g −1 of the dw of leaves, respectively. At the same sampling points, the number of MH8a cells in the roots of S. alba reached a value of 8.6 × 10 3 and 9.2 × 10 3 cfu g −1 of the dw of roots, respectively.

DISCUSSION
The plant-bacteria partnership can be applied to increase the phytoremediation efficiency of soil and water contaminated with organic and/or inorganic pollutants (Weyens et al., 2009;Khan et al., 2015). The results of many studies revealed that phytoextraction may be significantly enhanced by various bacteria including PGPB (Jiang et al., 2008;Rajkumar and Freitas, 2008;Dimkpa et al., 2009;Ma et al., 2009;Płociniczak et al., 2013a). Recently, attention has concentrated on the bioaugmentation of soil with bacteria that are characterized by metal resistance and plant growth-promoting traits (Ma et al., 2015).
In the present study, we describe the metal-tolerant bacterial strain B. casei MH8a, which was isolated from the rhizosphere of S. alba that was growing in heavy metal contaminated soil, as a potential agent in the enhancement of phytoextraction. Moreover, this strain was able to establish a close interaction with S. alba by colonizing its tissues.
Endophytic bacteria may improve plant tolerance to heavy metals and plant growth through several biological mechanisms. The beneficial effects of endophytic bacteria are generally attributed to the utilization of ACC, the production of IAA and siderophores and the solubilization of phosphates (Kolbas et al., 2015). Bacterial ACC deaminase can limit the level of the stress hormone ethylene in plants that are growing in harsh conditions thus increasing a plant's growth. Additionally, the secretion of phytohormones such as IAA can lead to the formation of ACC, which in turn may be converted into ammonium and α-ketobutyrate by endophytic bacteria. The increase in the Roots 1331 * ± 22 20.9 * ± 0.5 5 1 .3 * ± 1.2 * p < 0.01.
biomass and metal accumulation in plants that was observed in our studies may be connected with the biochemical features of the MH8a strain. Biochemical analysis of MH8a revealed the activity of several mechanisms that may potentially be responsible for the promotion of plant growth and indirectly for the enhancement of phytoextraction. The ACCD activity at the level of 53.6 ± 1.47 nmol α-ketobutyrate mg −1 h −1 , IAA production (3.44 ± 0.09 μg IAA mL −1 ) as well as the release of ammonia and hydrocyanic acid allows the MH8a strain to be classified as a PGPB. It is very difficult to connect the positive effect of MH8a on plant growth and heavy metal phytoextraction with a specific bacterial mechanism. For example, it has been observed that a low level of ACCD activity, approximately ≥20 nmol α-ketobutyrate mg −1 protein h −1 , is sufficient to enable a bacterium to grow on ACC and to act as a PGPB. Interestingly, organisms with a higher ACCD activity (300-400 nmol α-ketobutyrate mg −1 protein h −1 ) do not necessarily promote root elongation more than the strains that are characterized by a lower enzyme activity (Penrose and Glick, 2003). The secretion of siderophores and phosphate solubilization are also considered to be features of PGPB, but MH8a did not exhibit these activities. Such bacterial mechanisms are essential in niches that are poor in bioavailable forms of iron and phosphorus. During the phytoextraction experiment, symptoms of an Fe and P deficiency were not observed in S. alba indicating that bacteria and plants did not suffer from a lack of these nutrients.  The effect of MH8a on plant biomass was expressed in the significant increase of S. alba weight in the plants that were cultivated in soil treated with the tested strain. This result is in agreement with previous reports describing a greater biomass of S. alba growing in soil treated with Enterobacter intermedius MH8b and several Pseudomonas strains that had also been isolated from heavy metal contaminated soil (Płociniczak et al., 2013a,b). The positive impact of endophytic bacteria on plant biomass has been confirmed by several authors. For instance, Chen et al. (2014) found that the biomass of Sedum alfredii treated with the Sphingomonas SaMR12 strain was significantly higher as compared with a non-inoculated control. Similarly, Kolbas et al. (2015) confirmed the positive effect of endophytic bacteria (mainly from Bacillus and Rhodococcus genera) isolated from seeds of Agrostis capilaris on the biomass of inoculated plants grown in soil contaminated with Cu (13-114 mg kg −1 ). They found that at these Cu concentrations, endophytic inoculants increased shoot biomass between 1.6 and twofold compared to the control plants.
Plants that might be useful in the phytoextraction process, besides possessing a high biomass, should uptake and accumulate heavy metals with a high efficiency. The efficiency of the phytoextraction of metal-contaminated soil can be enhanced by metal-tolerant PGPE (Mastretta et al., 2009;Ma et al., 2011b). In our study we observed that the introduction of the multi-metalresistant strain MH8a into soil resulted in a significantly higher accumulation of Zn, Cd, and Cu in the shoots and roots of S. alba. In comparison with the control plants, the amount of Zn, Cd, and Cu in the shoots of inoculated plants was 87, 207, and 39% higher, respectively. The positive role of metal-resistant PGPE in the enhancement of phytoextraction by Sedum plumbizincicola was reported earlier by Ma et al. (2015). Among the tested strains (Bacillus pumilus E2S2, Bacillus sp. E1S2, Bacillus sp. E4S1, Achromobacter sp. E4L5 and Stenotrophomonas sp. E1L), the most effective strain, B. pumilus E252, significantly increased the fresh and dry biomass of plants (37 and 32%, respectively), as well as the plant Cd uptake (43%). In other studies Ma et al. (2011b) showed that the PGPE Pseudomonas sp. A3R3 significantly increased the Ni accumulation in Alyssum serpyllifolium during microbial-assisted phytoremediation. Similarly, Mastretta et al. (2009) confirmed the positive effect of Cd-resistant Sanguibacter sp. on Cd phytoextraction by Nicotiana tabacum. Plants growing in soil treated with this strain accumulated a significantly higher amount of Cd, as compared to control plants. It is worth emphasizing that MH8a caused the higher translocation of Cd from the roots to the shoots of plants was confirmed by the higher value of the TF for cadmium (0.91) as compared to the control plants (0.56). This result in combination with the high TF for Zn (1.03) makes the system of white mustard and B. casei MH8a especially useful for the phytoextraction of soil contaminated with zinc and cadmium.
It is widely accepted that the inoculants that are used in bioaugmentation should not upset the microbial equilibrium in soil; however, very little is known about the impact of PGPB/PGPE on the indigenous microbial communities in soil. This is particularly important because most of the bacteria that are used in the bioaugmentation process are introduced directly into soil.
In our study the bacterial communities of both the control and inoculated soils were dominated by Gram-negative bacteria. As was indicated by PLFA analysis, the introduction of MH8a caused temporary changes in the structure of the bacterial populations in inoculated soils. The temporary changes in the percentage of branched fatty acids in the PLFA profiles probably resulted from the introduction of the Gram-positive MH8a strain. However, the changes decreased over the period of experiment. This was observed because MH8a was isolated from the same heavy-metal polluted soil that was used in the phytoextraction experiment and was a member of the autochthonous population of microorganisms.
The strain used in our study had the ability to enter a plant's tissues and become an endophyte. One of the problems connected with the use of endophytes as an inoculum is their poor survival in soil and weak recolonization of plants, which are caused by harsh soil conditions and competition between bacterial species. It seems that the bioaugmentation of soil with metal-tolerant PGPB, which have originated from the rhizosphere or bulk soil and are able to colonize plant tissue is an alternative that may reduce the risk of the poor survival of an inoculant.
For the effective enhancement of phytoextraction by bacteria, the bacteria that are introduced should interact closely with plants and exhibit the ability to colonize the rhizosphere and/or interior of plant tissues. The plant apoplast offers different growth conditions, and therefore, different strains originating from rhizosphere can efficiently colonize the plant interior and become endophytes (Khan et al., 2015).
The strain that was used in this study was able to colonize both the rhizosphere soil and plant tissues and this interaction supported the phytoextraction of heavy metals. Seven days after the inoculation of the soil with the MH8a strain, rifampicinresistant bacteria were detected in the rhizosphere as well as in the roots and leaves of S. alba. Similar experiments on bacterial survival in the rhizosphere soil and tissues of apple seedlings was evaluated by Ju et al. (2014), who studied the ability of Bacillus subtilis Y-1 to colonize and protect apple seedlings against Fusarium oxysporum. Using the plating method, the authors showed that the rifampicin-resistant strain of Y-1 could colonize the rhizosphere and plant tissues within 30 days. The colonization of potato plants by rifampicin-resistant strains of Paenibacillus sp. E119 and Methylobacterium mesophilicum SR1.6/6 was tested by Andreote et al. (2010). The authors emphasized the role of the shifts in the compositions of plant-associated communities after the successful colonization of plant tissues by the bacterial inoculant. These shifts may lead to differences in the plant's metabolism thereby influencing plant growth and crop yields.

CONCLUSION
A metal-tolerant B. casei MH8a strain, due to the activity of several mechanisms that are considered to be important for plant growth-promotion, has the potential to enhance the phytoextraction of heavy metals from contaminated soil. Additionally, MH8a showed a high survival rate after introduction into the soil and was able to colonize the internal tissues of S. alba. These features indicate that MH8a in combination with S. alba can be regarded as an effective tool for the phytoextraction of Cd and Zn.

AUTHOR CONTRIBUTIONS
All authors listed, have made substantial, direct and intellectual contribution to the work, and approved it for publication.

ACKNOWLEDGMENT
The research was supported by grant No. 2013/11/B/NZ9/00152 financed by the National Science Centre, Poland.