AUTHOR=Wang Xiaolei , Wu Dezhi , Yang Qian , Zeng Jianbin , Jin Gulei , Chen Zhong-Hua , Zhang Guoping , Dai Fei TITLE=Identification of Mild Freezing Shock Response Pathways in Barley Based on Transcriptome Profiling JOURNAL=Frontiers in Plant Science VOLUME=7 YEAR=2016 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2016.00106 DOI=10.3389/fpls.2016.00106 ISSN=1664-462X ABSTRACT=
Low temperature is a major abiotic stress affecting crop growth and productivity. A better understanding of low temperature tolerance mechanisms is imperative for developing the crop cultivars with improved tolerance. We herein performed an Illumina RNA-sequencing experiment using two barley genotypes differing in freezing tolerance (Nure, tolerant and Tremois, sensitive), to determine the transcriptome profiling and genotypic difference under mild freezing shock treatment after a very short acclimation for gene induction. A total of 6474 differentially expressed genes, almost evenly distributed on the seven chromosomes, were identified. The key DEGs could be classified into six signaling pathways, i.e., Ca2+ signaling, PtdOH signaling, CBFs pathway, ABA pathway, jasmonate pathway, and amylohydrolysis pathway. Expression values of DEGs in multiple signaling pathways were analyzed and a hypothetical model of mild freezing shock tolerance mechanism was proposed. Expression and sequence profile of