The Transcription Factor AtDOF4.7 Is Involved in Ethylene- and IDA-Mediated Organ Abscission in Arabidopsis

Organ abscission is an important plant developmental process that occurs in response to environmental stress or pathogens. In Arabidopsis, ligand signals, such as ethylene or INFLORESCENCE DEFICIENT IN ABSCISSION (IDA), can regulate organ abscission. Previously, we reported that overexpression of AtDOF4.7, a transcription factor gene, directly suppresses the expression of the abscission-related gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (ADPG2), resulting in a deficiency of floral organ abscission. However, the relationship between AtDOF4.7 and abscission pathways still needs to be investigated. In this study, we showed that ethylene regulates the expression of AtDOF4.7, and the peptide ligand, IDA negatively regulates AtDOF4.7 at the transcriptional level. Genetic evidence indicates that AtDOF4.7 and IDA are involved in a common pathway, and a MAPK cascade can phosphorylate AtDOF4.7 in vitro. Further in vivo data suggest that AtDOF4.7 protein levels may be regulated by this phosphorylation. Collectively, our results indicate that ethylene regulates AtDOF4.7 that is involved in the IDA-mediated floral organ abscission pathway.


INTRODUCTION
Plant floral organ abscission is an important developmentally controlled process. Arabidopsis thaliana is an ideal model in which to study organ abscission (Bleecker and Patterson, 1997). In Arabidopsis, at the bases of the filaments, petals, and sepals in the floral organ, there are some small, high-density cell layers known as abscission zone (AZ), where the abscission process takes place (Patterson, 2001). The AZ cells perceive the abscission signal and subsequently activate cell walldegrading proteins. Finally, the pectin-rich middle lamellae of AZ cell walls are dissolved, resulting in the detachment of unwanted organs from the main plant body (Patterson, 2001;Patterson and Bleecker, 2004;Niederhuth et al., 2013a).
Several reports have indicated that abscission can be regulated in an ethylene-independent manner. It was originally found that the important plant hormone auxin could regulate the differentiation of the AZ of the leaf rachis in Sambucus nigra through transportation of auxin from the organ distal to the AZ (Osborne and Sargent, 1976;Morris, 1993). In Arabidopsis, auxin negatively regulates several polygalacturonases (PGs) in the dehiscence zone (DZ) to cause a delay in cell separation (Dal Degan et al., 2001;Ellis et al., 2005). Mutation of Auxin Response Factor 2 (ARF2) can lead to a delay in floral organ abscission. Furthermore, the effect of a delay in abscission can be enhanced by mutations in ARF1, ARF2, NPH4/ARF7, and ARF19 (Ellis et al., 2005;Okushima et al., 2005), suggesting that these overlapping genes regulate auxin-mediated floral organ abscission in an ethylene-independent manner.
Other than phytohormone, signaling peptides also play important roles in the regulation of abscission. In Arabidopsis, a short peptide, INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) has a key role in the regulation of floral organ abscission (Butenko et al., 2003;Liu et al., 2013). The ida single mutant fails to abscise its floral organs (Butenko et al., 2003), and constitutive expression of IDA in Arabidopsis (35S:IDA) induces premature organ abscission . Two leucine-rich repeat receptor-like protein kinases (LRR-RLKs), HAE and HSL2, are required for organ abscission. Double mutants for these two genes display organ abscission defects (Jinn et al., 2000;Cho et al., 2008;Stenvik et al., 2008;Niederhuth et al., 2013b). As previously reported, Arabidopsis perceives and responds to environmental signals through an interaction between extracellular peptides and plasma membrane-bound RLKs (Stenvik et al., 2008). IDA may act as a ligand binding to the HAE/HSL2 RLK, which transmits the abscission signal to downstream substrates to initiate organ abscission Stenvik et al., 2008).
Mitogen-activated protein kinase (MAPK) cascades play crucial roles in regulating various plant responses. There are two MEKs (MKK4 and MKK5) that are involved in floral organ abscission in Arabidopsis . Their downstream target substrates are MPK3 and MPK6 (Ren et al., 2002;Cho et al., 2008). MKK4 and MKK5 RNA interference (RNAi) transgenic lines show pleiotropic effects, including organ abscission deficiency, and constitutively active mutants of MKK4 DD (T224D/S230D) and MKK5 DD (T215D/S221D) that strongly activate endogenous MPK3 and MPK6 can restore abscission in the ida-2 and hae hsl2 mutants (Ren et al., 2002;Cho et al., 2008). Although the mpk3 and mpk6 single mutants exhibit normal organ abscission, the mpk3 mpk6 double mutant is lethal , while functionally inactive forms of MPK6 are expressed in mpk3 mutant plants, mpk3/MPK6 KR (Lys, the key residue of phosphate transferring, mutated to Arg) or mpk3/MPK6 AF (the conserved sites of phosphorylation by MKK, Thr and Tyr, mutated to Ala and Phe, respectively), survive and display loss of organ abscission . Furthermore, MPK6 has reduced kinase activity in hae hsl2 and ida-2 mutants. These results demonstrate that MPK6, but not MPK3, plays a dominant role in the regulation of floral organ abscission . In the ethylene-independent abscission pathway, IDA couples with HAE and HSL2, activating the downstream MAPK cascade to phosphorylate the substrates and initiate the separation of the AZ cells Shi et al., 2011;Niederhuth et al., 2013a,b).
Generally, ethylene regulates the timing of floral organ abscission, while IDA influences the degree of abscission. The physiological process of floral organ abscission is generally divided into four stages: (1) differentiation and formation of the AZ; (2) transduction of abscission signals; (3) activation of the abscission process; and (4) post-abscission transdifferentiation (Patterson, 2001;Niederhuth et al., 2013a). The second and third stages of abscission, in which ethylene, IDA, HAE/HSL2, MAPKs and PGs play important roles, have been well-described (Niederhuth et al., 2013a). However, further investigation is needed to understand how these two stages are linked.
We previously reported that AtDOF4.7, a member of the Arabidopsis DNA binding with one finger (DOF) transcription factor family, functions as an abscission inhibitor to directly regulate the expression of ADPG2, which encodes a cell wallhydrolyzing enzyme, to initiate cell separation (Wei et al., 2010). Nevertheless, it is still an open question whether AtDOF4.7 is a factor that acts between the second and third stage of abscission, potentially in a cascade of abscission signals that are transmitted from the second to the third stage through AtDOF4.7. In the present study, we demonstrated that AtDOF4.7 is an additional component of the IDA-mediated abscission pathway, and that it is regulated by ethylene and IDA. Our results suggest that AtDOF4.7 is regulated by both the ethylene-dependent and ethylene-independent pathways.

Plant Material
Arabidopsis thaliana (Columbia-0 ecotype) plants were grown at 22 • C in a growth chamber under a 16 h light/8 h dark photoperiod and a light intensity of 120 µmol m −2 s −1 .
To analyze the expression pattern of AtDOF4.7 in response to ethylene, 4-weeks-old Promoter AtDOF4.7 ::GUS plants were maintained in an air-tight growth chamber with or without ethylene at 10 ppm (10 µl l −1 ) for 3 days prior to the analysis.
To observe the abscission phenotype of S107/MKK5 DD , siliques of S107/MKK5 DD plants were treated with or without 0.02 µM DEX (Sigma, USA) for 24 h to induce MKK5 DD expression. The siliques were then photographed with a Cannon G12 camera. Detached leaves from S107/MKK5 DD plants were immersed in 15 µM DEX for various times prior to western blotting. Each treatment was repeated at least three times.
β-Glucuronidase (GUS) Assay β-Glucuronidase (GUS) gene expression was analyzed by staining different flower positions along the inflorescence as described previously (Wei et al., 2010). Histochemical GUS staining in the AZ cells of siliques was observed with an Olympus SZX16-DP72 stereo microscope system.

Quantitative Real-Time and Semi-quantitative RT-PCR
For semi-quantitative RT-PCR analysis, total RNA samples were extracted from Arabidopsis siliques using the RNeasy R Plant Mini Kit (Qiagen, Germany), and the RNA was reversely transcribed into cDNA with a Reverse Transcription System (Promega, USA). Semi-quantitative RT-PCR assays were run in a MJ Mini Personal Thermal Cycler (Bio-Rad, USA) with 25 thermal cycles. ACTIN2 (At3g18780) was employed as an internal control.
For quantitative real-time RT-PCR (qRT-PCR) analysis, total RNA was extracted from whole flowers and siliques, and qRT-PCR assays were run using the 7500 Real-Time PCR System (ABI, USA). After normalization to an internal control (ACTIN2; Charrier et al., 2002), the relative levels of gene expression were calculated via the Delta-Delta Ct (2 − Ct ) method (Livak and Schmittgen, 2001). All primers are given in online Supplementary  Table S2. Each relative gene expression assay was repeated at least three times.

Yeast Two-Hybrid Experiment and BiFc
A yeast two-hybrid (Y2H) experiment was performed as described previously (Wei et al., 2010). Cells that had been cotransformed with the pAD-GAL4-AtDOF4.7 (Wei et al., 2010) and pBD-GAL4-MPK3/MPK6 constructs (Xu et al., 2008) were cultured and transferred to 5 mM 3-AT medium lacking Trp, Leu, His, and Ade, followed by incubation at 28 • C for 3 days. Living yeast colonies were photographed with a Cannon G12 camera.

Preparation of Recombinant Proteins
Escherichia coli cells (strains BL21 and DE3) were transformed with the MPK3/6-pET30a(+)-His and MKK5 DD -pET28a(+)-FLAG constructs and then incubated as described previously (Liu and Zhang, 2004). His-tagged MPK3/6 recombinant proteins and FLAG-tagged MKK5 DD recombinant proteins were purified as described elsewhere (Xu et al., 2008). The AtDOF4.7 CDS was cloned into pMal-c2x (NEB, USA) in frame with the N-terminal MBP tag, and the construct was transformed into the E. coli strain Rosetta (DE3). Recombinant protein expression was induced with 0.1 mM isopropylthio-β-galactoside (IPTG) for 8 h at 18 • C. The MBP-tagged protein was purified using amylose resin (NEB, USA) according to the manufacturer's instructions.

Protein Extraction and Western Blotting Assay
Total protein was extracted from Arabidopsis silique tissues as described previously . The protein concentration was determined with a Bio-Rad protein assay kit (Bio-Rad, USA), using bovine serum albumin (BSA) as the standard. For western blotting assay, total protein extracts from each sample (10 µg per gel lane) were separated on 12% SDS-polyacrylamide gels. After electrophoresis, the proteins were electro-transferred to PVDF membranes (Millipore, USA). The membranes were subsequently incubated with an anti-Flag antibody (Cell Signaling, 1:10,000 dilution), washed, and then incubated with HRP-conjugated goat anti-rabbit IgG as a secondary antibody (1:10,000 dilution). The resultant protein bands were visualized by treating the membrane with the Immobilon TM Western Chemiluminescent HRP Substrate (Millipore, USA), following the manufacturer's instructions. Bands intensities in the western blots were compared using ImageJ software.

Phosphorylation of AtDOF4.7 In Vitro
Recombinant MPK3 (3.2 µg) and MPK6 proteins (5.7 µg) were activated by incubation with recombinant MKK5 DD (0.36 µg) in the presence of 50 µM ATP in 50 µl of reaction buffer (20 mM Hepes, pH 7.5, 10 mM MgCl 2 , and 1 mM DTT) at 25 • C for 1 h. The activated MPK3 and MPK6 proteins were then used to phosphorylate the AtDOF4.7 protein (1.86 µg, 1:16 enzyme:substrate ratio) in the same reaction buffer containing 25 µM ATP and [γ-32 P] ATP at 25 • C for 30 min, according to Liu and Zhang (2014). The reaction was stopped by the addition of SDS gel-loading buffer. Using the Mini-PROTEAN R Tetra System (Bio-Rad, USA), the samples were separated via electrophoresis on a 10% SDS-polyacrylamide gel at a constant 120 V. The gel was then dried under vacuum, and the phosphorylated AtDOF4.7 was visualized through autoradiography. Primary anti-His (TIANGEN, China) and anti-Flag (Cell Signaling Technology, USA) antibodies were used to verify the presence of the corresponding proteins in the phosphorylation buffer. Pre-stained protein markers (Fermentas, USA) were employed to calculate the molecular masses of the phosphorylated proteins.

Ethylene Regulates the Expression of AtDOF4.7
A previous study indicated that overexpression of AtDOF4.7 induces abscission defects in an ethylene-independent manner, although the flowers can perceive ethylene, suggesting that AtDOF4.7 might not be a direct target of early ethyleneindependent signal transduction (Wei et al., 2010). To investigate the role of AtDOF4.7 downstream of the early abscission pathway, we examined the expression pattern of Promoter AtDOF4.7 ::GUS in the mutants with ethylene-insensitive and ethylene-independent abscission deficiencies.
In the earlier report, the first flower showing visible white petals was referred as flower position 1 and then counted downward along the inflorescence in sequence (Wei et al., 2010). We initially crossed a plant carrying a single-copy of the Promoter AtDOF4.7 ::GUS construct with A. thaliana Col-0 (wildtype). The GUS expression pattern in the Promoter AtDOF4.7 ::GUS plant was similar to that reported previously ( Figure 1A). GUS expression was observed in the AZ cells at flower position 4, while at position 20, GUS expression from the AtDOF4.7 promoter could scarcely be detected in the AZ cells.
The Promoter AtDOF4.7 ::GUS plants were then crossed with the ethylene-insensitive mutant ein2-1. The expression pattern of Promoter AtDOF4.7 ::GUS in the ein2-1 mutant was altered temporally (Figures 1B,D and Supplementary Figure S3B), although spatial expression was similar to that of the wild type (WT). GUS expression was first observed at position 8 and continued to accumulate until reaching its maximum level at position 14. At position 20, GUS expression was still detectable in the AZ cells ( Figure 1B).
The expression pattern of Promoter AtDOF4.7 ::GUS in the etr1-1 mutant was also examined, and we found that the temporal expression pattern was altered in this mutant as well (Supplementary Figure S1). GUS expression was detectable at later flower positions than in Promoter AtDOF4.7 ::GUS/ein2-1 flowers and was faintly visible at position 10. Expression was clearly observed at position 20. In addition, the profile of the spatial expression of AtDOF4.7 in the etr1-1 mutant was similar to that in ein2-1 (Figure 1B and Supplementary Figure S1).
Temporally, the expression of Promoter AtDOF4.7 ::GUS in AZ cells in the ethylene-insensitive ein2-1 and etr1-1 mutants was remarkably delayed compared with that in WT. Because, flower abscission is also delayed in the inflorescences of the ein2-1 and etr1-1 mutants (Butenko et al., 2003;Patterson and Bleecker, 2004), we concluded that ethylene can influence AtDOF4.7 expression. To confirm this finding, we placed transgenic plants expressing Promoter AtDOF4.7 ::GUS in an air-tight chamber containing 10 ppm ethylene gas, which can accelerate organ abscission. Compared with the Promoter AtDOF4.7 ::GUS plants that were exposed to air (in the absence of ethylene), GUS expression was detected earlier at flower position 1 in the presence of ethylene (Supplementary Figure S2). The timecourse of Promoter AtDOF4.7 ::GUS expression was shortened by ethylene treatment. These experimental results indicated that ethylene can influence the timing of AtDOF4.7 expression, and the time-course of AtDOF4.7 expression could be relevant to organ abscission.

AtDOF4.7 Is a Component of the IDA-Mediated Abscission Pathway Underlying Abscission
In the IDA-mediated abscission pathway in Arabidopsis, IDA binds as a ligand to the RLKs HAE and HSL2 (Stenvik et al., 2008). Some cytoplasmic effectors, such as MKK4, MKK5, MPK3, and MPK6, function together to control cell separation during abscission . As reported previously, IDA can control the intensity of organ abscission (Butenko et al., 2003;Stenvik et al., 2006). To further determine whether the expression of AtDOF4.7 is regulated by IDA at the transcriptional level, we crossed a Promoter AtDOF4.7 ::GUS transgenic plant with the ida-2 (Col-0) mutant. The temporal expression pattern of Promoter AtDOF4.7 ::GUS was unaltered in the AZ cells of the siliques in the ida-2 background and was similar to that of AtDOF4.7 in the WT background (Figures 1A,C). However, qRT-PCR showed that the relative expression levels of AtDOF4.7 from flower positions 6 to 10 were significantly higher in the ida-2 background that in WT ( Figure 1D and Supplementary Figure S3A). These data suggested that IDA negatively regulates the expression of AtDOF4.7 at the transcriptional level.
To confirm the hypothesis that IDA and AtDOF4.7 are involved in a common pathway, we crossed an AtDOF4.7overexpressing line (S107) with 35S:IDA in the Col-0 background, which shows early abscission of the flowers at position 3 . Semi-quantitative RT-PCR demonstrated that both the AtDOF4.7 and IDA genes were overexpressed in the siliques of S107/35S:IDA plants (Figure 2A). The S107/35S:IDA lines showed that the floral organ abscission defect phenotype was similar to that in S107 plants (Figures 2D,E). Most notably, although the transcript levels of IDA were higher than that of AtDOF4.7 in S107/35S:IDA lines, from which the floral organs could not shed (Figures 2A,E). This data suggested that AtDOF4.7 might be epistatic to IDA. The flowers of WT plants exhibited normal abscission at position 6 ( Figure 2B). After abscission beginning at position 6, excessive secretion of arabinogalactan protein (AGP) was observed in the AZ cells of the flowers of 35S:IDA plants ; Figure 2C). However, there was no AGP observed in the AZ cells of siliques of S107/35S:IDA plants, even after the floral parts on the fruits were removed from positions 6 to 20 ( Figure 2F). These results support the hypothesis that IDA and AtDOF4.7 act in a common pathway to regulate abscission, and IDA might be in the upstream abscission pathway.

Interaction of AtDOF4.7 with MPK3 and MPK6 In Vivo and In Vitro
A previous study found that the MPK6 KR /mpk3 mutant, but not mpk3 or mpk6 single mutants, showed a defective floral abscission phenotype . Because MPK3 and MPK6 are key components of the IDA-mediated pathway underlying abscission, to understand how AtDOF4.7 is regulated The temporal expression pattern of AtDOF4.7 in WT was the same as in ida-2. However, the relative expression of AtDOF4.7 in ida-2 was higher from positions 6 to 10 than in WT. At flower positions 14 and 16, the expression level of AtDOF4.7 in the ida-2 background was similar to WT. Compared with WT, the temporal expression pattern of AtDOF4.7 was delayed in the ein2-1 mutant. The relative expression level of AtDOF4.7 in ein2-1 from positions 2 to 12 was significantly lower than in WT. After position 12, the relative expression level of AtDOF4.7 was elevated >2-fold in the ein2-1 compared with WT. Relative mRNA levels were averaged over three biological replicates and are shown with the SD (error bars).
Frontiers in Plant Science | www.frontiersin.org  by the MAPKs, we performed yeast two-hybrid (Y2H) and BiFC assays to determine whether AtDOF4.7 interacts with MPK3 or MPK6. The Y2H experiment showed that the transformed yeast cells were able to grow on 5 mM 3-AT medium lacking Trp, Leu, His, and Ade, suggesting an interaction between pAD-GAL4-AtDOF4.7 and pBD-GAL4-MPK3/MPK6 in vivo (Figure 3A). To further confirm the interaction of AtDOF4.7 with MPK3 or MPK6, we constructed the BiFC vectors pUC-SPYNE-AtDOF4.7 and pUC-SPYCE-MPK3/6, and the two constructs were cotransformed into Arabidopsis mesophyll protoplasts. The cells were then observed using laser fluorescence confocal microscopy; which showed that pUC-SPYNE-AtDOF4.7 and pUC-SPYCE-MPK6 interact in the nuclei ( Figure 3B). However, the yellow fluorescence could not be detected from the cells containing pUC-SPYNE-AtDOF4.7 and pUC-SPYCE-MPK3. The BiFC results strongly suggested that AtDOF4.7 and MPK6 physically interact in Arabidopsis.

Phosphorylation of AtDOF4.7 by MPK3 and MPK6 In Vitro
Potential sites for MAPK phosphorylation are Ser/Thr residues followed by a Pro residue (Meng et al., 2013). There are two such sites in the AtDOF4.7 sequence: Ser-34-Pro and Ser-104-Pro ( Figure 4A). To determine whether MPK3/MPK6 is able to carry out phosphorylation of AtDOF4.7, we obtained the recombinant proteins MBP-tagged AtDOF4.7, His 6tagged MPK3 and MPK6, and FLAG-tagged MKK5 DD for phosphorylation assays. Following activation by purified FLAGtagged recombinant MKK5 DD , either His 6 -tagged MPK3 or MPK6 strongly phosphorylated AtDOF4.7 (Figure 4B). FIGURE 5 | AtDOF4.7 is involved in abscission mediated by the MAPK cascade. The defective abscission defective phenotype of S107 was partly restored in S107/GVG-MKK5 DD plants following DEX treatment. All representative siliques are from flower position 10 of WT, S107, GVG-MKK5 DD , and S107/GVG-MKK5 DD plants.
Constitutively Activated MKK5 Cannot Rescue Organ Abscission in the S107 Line MKK4 and MKK5 are homologs found in Arabidopsis that act upstream of MPK3 and MPK6 in the regulation of floral abscission . Plants expressing the constitutively activated forms of MKK4 or MKK5 under the control of a steroid-inducible promoter (designated GVG-MKK4 DD or GVG-MKK5 DD ) had a normal abscission phenotype (Ren et al., 2002;Cho et al., 2008). To assess the biological function of the phosphorylation of AtDOF4.7 by MAPK, we crossed the AtDOF4.7-overexpressing S107 line with a GVG-MKK5 DD transgenic plant to obtain S107/MKK5 DD . We examined the siliques of S107/MKK5 DD plants at flower position 10, which is the position at which WT flowers shed completely. We observed that the organ abscission phenotype of S107/MKK5 DD was not rescued; however, small parts of the flowers were shed from the siliques of S107/MKK5 DD plants after dexamethasone (DEX) treatment ( Figure 5). These data indicated that the two genes act in a common pathway to regulate abscission in Arabidopsis, and MKK5 may negatively regulate AtDOF4.7.
A phosphorylation-dependent mechanism is involved in protein degradation (Willems et al., 1999;Feng et al., 2004;Yoo et al., 2008). Therefore, a western blot assay was performed to determine the changes in AtDOF4.7 protein levels in S107/MKK5 DD plants treated with DEX. The siliques of S107/MKK5 DD at flower position 10 were induced with 15 µM DEX after 10 h, resulting in greatly decreased AtDOF4.7 protein levels ( Figure 6A). The grayscale ratio of AtDOF4.7 protein bands on the western blot were measured, and the results showed that the changes in the levels of the AtDOF4.7 protein exhibited significant differences after 10 and 12 h of DEX treatment compared with the untreated control ( Figure 6B). ACTIN2 was used as an internal protein control, and was detected with an anti-ACTIN2 antibody. These data suggest that activated MKK5 following DEX induction could affect AtDOF4.7 at the protein level in the S107/MKK5 DD plant, providing one explanation for how organ abscission is partially recovered in S107/MKK5 DD lines.

DISCUSSION
A recent report shows that ethylene manipulates the rate of abscission, rather than directly inducing it (Niederhuth et al., 2013a). It has been indicated that several ethylene mutants of Arabidopsis, such as those harboring the ethylene receptor ethylene-response 1 (etr1) and ethylene-insensitive 2 (ein2) point mutations, displayed a delay of floral organ abscission (Bleecker et al., 1988;Guzman and Ecker, 1990;Patterson and Bleecker, 2004), however, the formation and differentiation of the AZ cells are not affected in these ethylene-insensitive mutants. Up to now, the genetic mechanism through which ethylene regulates abscission is still unclear. Here, we suggest that ethylene accelerates organ abscission in Arabidopsis by regulating the time-course of the expression of AtDOF4.7 (Figure 1B, Supplementary Figures S1, S2, and S3B). To make a preliminary demonstration for this hypothesis, we analyzed the region 3000 bp upstream of the AtDOF4.7 promoter sequence and found that the promoter sequence of AtDOF4.7 contained FIGURE 6 | Determination of AtDOF4.7 protein levels in S107/MKK5 DD siliques. (A) Without DEX treatment, the AtDOF4.7 protein levels did not change apparently over the 12 h time courses of the experiment. However, the level of the AtDOF4.7 protein appeared to be greatly decreased at the 10 and 12 h time points following DEX treatment. The bands indicated by arrows are the AtDOF4.7 protein. An anti-ACTIN2 antibody was used as the internal control. Total protein extracts from WT and S107 were used as the negative and positive controls, respectively. (B) The grayscale ratio of these bands without and with DEX treatment from (A). The asterisks indicate significant differences between DEX treatment and the control (-DEX) at the 10 and 12 h time points (t-test, P < 0.05). Grayscale measurements of each band were repeated three times. three cis-acting elements in this region: a GCC box (-1702 to -1696 bp), a CRT/DRE element (-1029 to -1023 bp) and several EIN3-binding sites (EBS, ATGTA; Supplementary Figure S4). The first two elements can be recognized by Ethylene Response Factors (ERFs) in response to ethylene (GCC) and drought stress (CRT/DRE), while the last element can be recognized by EIN3. Most notably, in the ethylene signaling pathway, ERFs are critical factors responding to ethylene, which can be activated by EIN3 (Ohme-Takagi and Shinshi, 1995;Chao et al., 1997). Based on these observations, AtDOF4.7 might be a potential target gene of ERFs or EIN3 in response to ethylene. From the data presented in this study, we hypothesize that the regulation of AtDOF4.7 is related to ethylene, but it is unclear that how ethylene affecting the expression of AtDOF4.7 at the transcriptional level.
Auxin, as an antagonist of ethylene, negatively regulates the cell wall-degrading enzymes, leading to a delay in cell separation via an ethylene-independent mechanism. Additionally, the AZ patterning is not affected in arf2 mutants (Ellis et al., 2005). It has been reported that auxin may regulate IDA and HAE/HSL2 to control cell separation (Kumpf et al., 2013;Niederhuth et al., 2013a). Thus, we can put forward the hypothesis that both ethylene and auxin may be involved in the regulation of AtDOF4.7 expression.
Previous data indicated that the delay of abscission caused by AtDOF4.7 overexpression could not be accelerated by exogenous ethylene, suggesting the potential involvement of ethylene-independent regulation. Therefore, we investigated the relationship between IDA and AtDOF4.7 in this study. In the mutant of IDA, the expression pattern of AtDOF4.7 promoter was not significantly altered ( Figure 1C), while the transcript level of AtDOF4.7 was found to be up-regulated at the same flower positions in the ida-2 mutant ( Figure 1D). IDA is involved in the regulation of ADPG2 expression in the AZ to control cell separation during floral organ abscission (González-Carranza et al., 2002Ogawa et al., 2009;González-Carranza et al., 2012), and its expression is suppressed by overexpression of AtDOF4.7 in the regulation of abscission (Wei et al., 2010). Our results indicated that the overexpression of AtDOF4.7 could inhibit the early abscission caused by IDA overexpressing lines (Figure 2). Therefore, this result provides a hint that AtDOF4.7 might participate in the regulation of PGs by IDA. It has been well-established that MAPK cascades (mainly MKK4/MKK5-MPK3/MPK6 pathway) are involved in downstream of IDAmediated abscission pathway. Thus, the relations between the MAPKs and AtDOF4.7 were also investigated. First, we found two abscission-related MPKs, MPK3 and MPK6, could interact with AtDOF4.7 in yeast. The in vitro assay indicated that both MPK3 and MPK6 could phosphorylated AtDOF4.7, and the signal of MPK6 was apparently stronger than that of MPK3. This is consistent with the stronger in vivo interaction between MPK6 and AtDOF4.7, suggesting MPK6 may play more important role in the regulation of abscission. Second, our results indicated that the AtDOF4.7-induced abortion of abscission could be partially rescued by MKK activation (Figure 5), and the protein level of AtDOF4.7 is down-regulated following the MKK5 activation. Thus, it is reasonable to speculate that the AtDOF4.7 may act as a direct target of MPKs in the abscission regulation. Taken together, our results suggested that the AtDOF4.7 should also involved in downstream of the IDA-MAPK-mediated abscission pathway.

CONCLUSION
AtDOF4.7, as a negative regulator in floral organ abscission, is regulated by the ethylene-dependent and ethylene-independent abscission pathways. It is suggested that AtDOF4.7 might be regulated by ethylene, and also by IDA, which represses expression of AtDOF4.7. Nevertheless, the relationship between phosphorylation and ethylene signaling remains unclear. A previous study showed that phosphorylated MPK3/6 activated by MKK4/5 further phosphorylates ERF6 to regulate defense gene induction and fungal resistance ( (Meng et al., 2013). Therefore, it is worthwhile to explore whether the phosphorylation of ERFs by MPK3/6 in the regulation of AtDOF4.7 occurs in response to ethylene. Further detailed biochemical and genetic analyses will allow us to gain a better understanding of the importance of AtDOF4.7 phosphorylation and ethylene responses in plant organ abscission.