Recent Developments in Systems Biology and Metabolic Engineering of Plant–Microbe Interactions

Microorganisms play a crucial role in the sustainability of the various ecosystems. The characterization of various interactions between microorganisms and other biotic factors is a necessary footstep to understand the association and functions of microbial communities. Among the different microbial interactions in an ecosystem, plant–microbe interaction plays an important role to balance the ecosystem. The present review explores plant–microbe interactions using gene editing and system biology tools toward the comprehension in improvement of plant traits. Further, system biology tools like FBA (flux balance analysis), OptKnock, and constraint-based modeling helps in understanding such interactions as a whole. In addition, various gene editing tools have been summarized and a strategy has been hypothesized for the development of disease free plants. Furthermore, we have tried to summarize the predictions through data retrieved from various types of sources such as high throughput sequencing data (e.g., single nucleotide polymorphism detection, RNA-seq, proteomics) and metabolic models have been reconstructed from such sequences for species communities. It is well known fact that systems biology approaches and modeling of biological networks will enable us to learn the insight of such network and will also help further in understanding these interactions.


INTRODUCTION
Microbial interactions have a decisive role in the sustainability of the various ecosystems. The characterization of such interactions among microorganisms and other biotic factors is a necessary footstep to understand the association and functions of microbial communities. Among the different microbial interactions in an ecosystem, plant-microbe interaction plays an important role to balance the ecosystem. Plants produce a number of organic and inorganic compounds which results in a nutritionally enriched environment which is favorable for heavy colonization of diversity of microbes. Microorganisms may colonize the exteriorly (epiphytes) or interiorly (endophytes). Microbial communities can affect the plant physiology either positively or negatively in direct or indirect ways by various interactions mutualism, commensalism, amensalism, and pathogenic consequences. Endophytic bacteria is an example of plant-microbe interaction wherein bacteria live in a non-competitive environment of host plant tissue without any major damage to the host cell (James and Olivares, 1998). Endophytes are omnipresent in nearly all plants on earth. Endophytic microflora such as bacteria and fungi, are defined as microorganisms which are present after surface sterilization of various plant parts such as root, shoot, seed, or nodules. It has been assumed that these endophytes originated from the rhizosphere, the seeds, and the aerial portion of plants (Seghers et al., 2004). The rhizospheric soil is a significant source of root endophytes (Gao et al., 2004;Castro-Sowinski et al., 2007;Imam et al., 2013a). These endophytic microbes are supposed to enter into the plant tissue by local fractures or cellulose degradation of the root system (Gough et al., 1997). Endophytes inside a plant tissue may either be restricted to the point of entry or extend throughout the plant. These bacteria generally colonize the intercellular spaces, and they have been isolated from all compartments including seeds. There are few studies on plant-microbe interactions on details about Avr protein, computational strategies for protein interactions, molecular diversity and interactions of virulence genes (Imam et al., 2013a(Imam et al., ,b,c, 2014(Imam et al., , 2015a. Both types of bacteria either Gram-positive or Gram-negative have been isolated from different tissues of numerous types of plant species. A number of facultative endophytes have been reported from rice, maize, wheat, sorghum, cotton, potato, and Arabidopsis. Furthermore, several different bacterial species have been isolated from a single plant. Conventionally, to investigate the various plant-microbial interactions use of a number of laborious laboratory experiments such as growth assays and pot house experiments are required (Kato et al., 2005;Harcombe, 2010;Zeidan et al., 2010). However, these laborious experiments make them infeasible for large scale application. With the help of bioinformatics approaches these issues can be alleviated by predicting plant-microbe interactions for experimental validation (Freilich et al., 2011;Buffie et al., 2014;Lima-Mendez et al., 2015). These predictions are founded on different types of informational data, such as the measurement of species abundances from high throughput sequencing or reconstructed metabolic models for species communities. There are several reports in various related fields where use of gene editing, genome engineering, and advanced technologies are proving quite significantly addressed Shukla, 2015a,b, 2016). In addition, various other in silico methods could be relevant to analyze such interactions while understanding the large amount of published data (Pritchard and Birch, 2011;Xu et al., 2013;Dix et al., 2016). This review envisages the concept of systems biology and gene editing in plant-microbe interactions by deciphering these technologies in detail.

PLANT-MICROBE INTERACTION AND ITS RELEVANCE
Microflora is an aggregation of several types of microbes to form heterogeneous communities which are necessary components in several ecological niches and composed of distinct proportions of various microorganisms. Microorganisms of microflora do not live isolated or independently, but in its place these populations actively interact with other biological members of the ecosystem within their ecological niche. These microbial interactions may take place with any of biological form such as animal-microbe interaction, microbe-microbe interaction, plant-microbe interaction, etc. Plants provide an excellent ecosystem for microbial interactions. The plant provides the variable environment to the microorganisms from aerial plant part to the stable root system for the interactions. On the basis of location of plant-microbe interaction, the microbes can be divided in two groups, phyllospheric microorganisms which interact with the aerial leaf surface of plants and rhizospheric which interact with roots of plants. Phyllospheric microorganisms are adapted to low humidity and high irradiation, helps to protect plants from airborne pathogens. Rhizosphere of plants is a nutritionally rich zone due to deposition of nutrition rich compounds such as amino acid, organic acid, vitamins, sugars, etc. secreted by the roots. There is a pictorial presentation of various microbiome in Figure 1 showing both phyllospheric and rhizospheric microorganisms. The nutritional enriched environment around roots creates a favorable environment for the growth of soil microorganisms, which includes rhizosphere and the rhizoplane soil microbial communities. A number of microorganisms interact with different plant tissues or cells with various level of dependence. These interactions may be beneficial, harmful, or neutral for one or both the organisms on the basis of this attribute plant-microbe interactions are known as amensalism (neutralnegative), antagonism (negative-positive), commensalism (neutral-positive), competition (negative-negative), mutualism (positive-positive), and neutralism (neutral-neutral). The commensalism or mutualism are more frequent interactions found in plants, in which either one or both species gain benefit from the relationship respectively (Campbell, 1995). Mycorrhiza and genus Rhizobium symbionts are best example of mutualism interaction. There are a number of superb reviews reporting present research on plant-microbe interaction at the molecular level, plant responses to quorum-sensing signals from microbial communities, applications of plant-microbe interaction, microflora responses toward transgenic plants and other rhizospheric interactions (Bauer and Mathesius, 2004;Singh et al., 2004;Sørensen and Sessitsch, 2007;Fillion, 2008;Ryan et al., 2008). The examination and understanding of these plant-microbe interactions helps to figure out the insights of mechanism which may direct us to understand such concerns. These sustainable resources will be ecofriendly and helpful to clean up the pollution and gaseous effect on a global scale.

Communication Systems
The life cycles of all the organisms from quorum sensing bacteria (Cornforth et al., 2014) to singing whales (Parks et al., 2014) are found on signaling pathways to convey information. Signaling system has played an important role in organismal evolution and the complexity of life (West et al., 2015). If both the donor as well as a receiver has a shared interest to propagate the reliable information then an effective signaling system can fetch a number of health benefits. The signaling pathway may be important from an evolutionary point of view because organisms can manipulate signals for their profit (Mokkonen and Lindstedt, 2015). Now these days, there has been an escalating awareness in communication network between the plants and root microflora which have a symbiotic relationship (Miller andOldroyd, 2012: Bakker et al., 2013;Andreo-Jimenez et al., 2015). The roots of plant are bordered by a massive amount of soil microorganisms consisting of tens of thousands species diversity (Bardgett and van der Putten, 2014). There should be an effective crosstalk between plant and surrounding microflora to establish a successful relationship. There should a better understanding of these molecular signaling pathways to access control over the microbial population. The researchers have made efforts from last decade to understand the molecular mechanism of communication in the rhizosphere (Guttman et al., 2014) but still we do not have sufficient knowledge to comprehend the evolutionary origins and stability of the rhizosphere communication system. Comprehension of major beneficial plant-microbe interactions such as arbuscular mycorrhizas and the plant growth promoting rhizobacteria (PGPR)-legume symbiosis have been changed over the past years. The PGPR-legume root symbiosis and arbuscular mycorrhizal (AM) symbioses are established by exchanging a number of signals as there is mutual identification of diffusible signal molecules generated from both plants and microbial partner. A common symbiotic pathway (CSP) is triggered by symbiotic signals produced by rhizospheric bacteria or fungi which are in form of lipo-chitooligosaccharides (LCOs). These LCOs are perceived via lysine-motif (LysM) receptors found on the plasma membrane of plant cell and activate the CSP which regulate the interactions between plant and rhizospheric microorganisms. LysM receptor families are found in both legume and nonlegume plants and receive signals from both rhizobia (Nod factor signals) and AM fungi (Myc-LCO signals). A model of CSP triggered in plants has been described in Figure 2 together with all the proteins and receptor molecules involved in signaling. Furthermore, in this review it has been tried to understand the signaling pathway among AM fungi and roots of their host plants, where organic food is exchanged for nutrients from soil. This symbiotic relationship is among the most prevalent and anticipated to have evolved roughly 450 Mya (Field et al., 2015). There are several evidences obtained that signaling pathways between AM fungi and roots of their plant hosts are so thriving that the components of this pathway have been recruited by plants to evolution of other symbiosis such as rhizobial N 2 -fixation (Geurts et al., 2012). Plants and microorganism use a signaling system to transmit information about their internal situation and their readiness for immigration or colonization, but how can these reach the desired recipients, and not others (Oldroyd, 2013). Theoretically, specific signaling is required at two levels a broader screening to identify or stimulating the mutualists and a finer screen, to distinguish high and low-quality strains within a mutualist microorganism (Werner and Kiers, 2015). Strigolactones are acting as a major plant signaling molecule in the symbiotic system of arbuscular mycorrhiza. Strigolactones are terpenoid lactones which are a byproduct of carotenoid metabolism (Bonfante and Genre, 2015). However, Strigolactones are plant hormones, which secondarily also act to attract AM fungi. Strigolactones act as a stimulus to initiate metabolic cycle of the AM fungus which promotes growth toward the roots (Figure 3; Gutjahr, 2014). The receptors for strigolactone in mycorrhizal fungi have not been yet discovered (Koltai, 2014) Different types of strigolactones have been emitted by different plants which vary from host to attract specific fungal species or strains (Conn et al., 2015). The germinating AM fungal spores were activated by strigolactones derived from a root which execute a series of signal molecules such as chitooligosaccharides and lipochitooligosaccharides. These signal molecules activate a set of reactions in the plant root system and consequently the cytosolic concentration of calcium boosts which further induces gene expression of activated AM fungi which directs to the creation of the pre-penetration apparatus. The reacting root will secrete cut-in monomers, signaling the fungi to form a hypopodium and initiate arbuscular growth (Padje et al., 2016). The PGPR is known to synthesize the phytohormones, auxins. Auxin production can occur via multiple pathways by both plants as well as PGPRs. There are certain papers available which report that indole-3-acetic acid (IAA) is a natural auxin acting as signaling molecules in microorganisms. IAA affects gene expression in some of microorganisms, thus IAA act as a reciprocal signaling molecule in microbe-plant interactions (Spaepen and Vanderleyden, 2011). The bacterial gene expression is regulated under the control of IAA has been first described for ipdC gene of Azospirillum brasilense. It has been reported that IAA act as an inhibitory signal molecule for viral gene expression by Agrobacterium tumefaciens a phytopathogen (Liu and Nester, 2006). Furthermore, auxin level in plant-PGPR interactions affects different levels of nodule formation in plants such as auxin transport inhibition by the flavonoids which act as indicators of specification of founder cell and auxins accumulations initiate the nodule formation and differentiation (Mathesius, 2008).

In silico Methods in Understanding Interactions
Systems biology is the study of genes, proteins and their interaction within a cell, tissue or whole organism. It also enables us to understand complex biological system and modeling it with the help of computational techniques. The interaction of host and pathogen in plants plays an important role in enhancing signaling cascade which brings change in the protein and eventually in the phenotypic expression. There are few notable studies on systems biology and molecular modeling tools to understand the microbial enzymes and similar proteins, but it lacks any further scope for studies of proteins involved in plant-microbe interaction Shukla, 2011, 2015;Karthik and Shukla, 2012;Baweja et al., 2015Baweja et al., , 2016Singh et al., 2016). The study of in silico transcriptomes of both host and pathogen during the infection will contribute to the knowledge of changes occurring during the infection. There are different database which is dedicated to host-pathogen interaction. There is dynamic complexity in the plant-microbe interaction which occurs since edges represent processes in biological networks that may take time to occur and are dependent on the other factors in the network. Concentrations of metabolites in metabolic and signaling processes vary over time thus there could be several ways to model this time-dependent variation. Ordinary differential equations are employed for the analysis and calculation of biochemical process for metabolic kinetics studies. In such studies edges and node forms the complex, edges are associated with some value of parameters such as binding coefficients. Edges comprise of values representing a quantity or concentration. There are variations in the value of nodes over the time as the substrate is utilized or byproduct is formed. Flux is the rate at which material flows, flux is associated with the edges and carry a certain value. Understanding flux and managing it helps in the regulating the biological process dynamics. The study of the dynamic behavior of interaction is complex to analyze even studying a small, dynamic behavior requires certain parameters and information which requires multiple dimension overview. The networks and their dynamic characteristics may be significant and these processes should be confirmed with valid experimental models. Topologies related to metabolomics of cell are dynamic between the compartments and they change over the time. It is obvious to mention here that concentrations (or counts) of active proteins, crucial metabolites within the interacting cell are more inconsistent than the topology of the metabolic model. This indicates a clear overview about that existence and these factors define the network topology. Furthermore, the amount of each active element in such system has varied significantly so such attributes are accessed by metabolomics, transcriptomics, and proteomics and these can be taken as significant markers to explicit the hostmicrobial interactions. There are examples in which microbes dominated the over the molecular control of the host and resulted in exceptional results including production of "zombie ants" and mimicry of flowers by the fungi Ophiocordyceps unilateralis (Pontoppidan et al., 2009) and Puccinia monoica (Roy, 1994). Such examples exemplify the potential of microorganisms to control elegantly the physiological processes in host cells. In it quite important to mention here that such microbes have developed the capacity toward environment control and influence the surrounding factors. The systems biology approach helps to find out various ways toward the alteration of host plant cells. There are not many chances that all the symptoms that appear in the plant-microbe interaction come out as a disease, it is just the coincidental part that occurs. All pathogens are causing disease will not be the right thing to consider. The pathogens which attack the host first explore the most vulnerable element of the host network that could cause more disruption in the most economical way. By virtue of this, the host also develops its defense system and the pathogen attack may be detected only in those parts of the system which are structurally most responsive to these changes. Further, it is to mention here that host cell will be benefited because the reduction in the number of receptor and recognition proteins. Systems biology approach and mathematical modeling of the system could also lead us to develop novel strategies to control the disease. Apart from these, the metabolism of plant engineered in microbe will show the way to the production of different essential components which are commercially important such as fuel and pharmaceutical molecules. An overall depiction of the methods described above is given in Figure 4.

Systems Biology Techniques for Deciphering Plant-Microbe Interaction
Metabolic engineering in microorganisms has been employed in different areas such as industrial microbiology, medical microbiology, and agricultural microbiology (Chotani et al., 2000;Nakamura and Whited, 2003). The targeted motive of metabolic engineering could be different, but the technology and platform remained unchanged. Recently, computational modeling emerged and changed the perspective to analyze metabolic engineering. Computational modeling anticipates the effect of genetic manipulations on metabolism, however, these methods need enzyme kinetic information that is still mostly unknown (Tepper and Shlomi, 2010). Constraint-based modeling (CBM), is an alternate which overcome these problems by examining the function of metabolic networks by relying on physical-chemical constraints (Price et al., 2003). There are certain genome-scale network models available for many microorganisms (Förster et al., 2003;Reed et al., 2003;Duarte et al., 2004). CBM has proved to be successful for large-scale microbial networks which involve metabolic engineering studies for different applications. A metabolic reconstruction is a wellstructured description of the network topology that enables derivation of genome-scale models (GEMs) that are used to mimic different metabolic states of an organism (Satish Kumar et al., 2007;Thiele and Palsson, 2010;Esvelt and Wang, 2013). Such technology has gained popularity for systems biology . The effectors act outside the host cell and sometimes secrete small molecules that may affect the host and modifies its biochemistry, for example, coronatine. We understand systems biology perspectives can be well applied to study such effectors and their pathogenesis aspects. These studies are based on certain tools which help in analyzing large amount of genomic data, interactions, GEMs this is depicted in Table 1. OptKnock is a technique which searches for sets of gene knockouts that lead to the production of desired products (Burgard et al., 2003) and can be used for the same purpose which can resist the plant from harmful microbial compounds. On the other hand, OptStrain that not only allows gene knockouts, but also incorporate novel enzymecoding genes from different species to a given microbial genome (Pharkya et al., 2004). More recently, OptReg was developed, searching for manipulations in the form of up-and downregulation of metabolic enzymes in addition to gene knockouts to meet desired metabolite production (Pharkya and Maranas, 2006).

Gene Editing: An Approach to Develop Customized Functions
The recombinant DNA technology has revolutionized the study of the genome to a next level to provide the opportunity for its application in various fields like agriculture, industries, etc. The techniques like gene editing are proving as potential techniques in improvement of crop characters such as enhancing yield, providing resistance from biotic and abiotic stress. This has been possible because of major gene editing tools like zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALEN), and clustered regularly interspaced short palindromic repeats (CRISPR-Cas) that introduce double strand break (DSB) in the target gene, which are repaired by the error-prone non-homologous end joining (NHEJ) pathway or homology-directed repair (HDR; Symington and Gautier, 2011).
ZFNs are artificial restriction enzymes that edit or cleave the specific target DNA by using zinc finger DNA-binding domain. The recognizing sequences viz. zinc finger domains can be artificially engineered to target specific sequences in the host. It consists of two DNA binding domains, the domain one is comprised of eukaryotic transcription factors and contain a zinc finger. The second domain includes the catalytic component, the nuclease FokI restriction enzyme that catalyzes the specific DNA sequences. ZFNs have successfully performed well in defining the functions of various genes from diverse organism, including proven highly valuable in defining the roles of numerous genes in cells from a variety of organisms, including fruit flies, humans, mice, and higher plants (Gaj et al., 2013). However, there are certain drawbacks of ZHN technology like difficulties in design, construction, cost, and uncertain success rates.
TALEN are restriction enzymes that cleave target DNA by utilizing TAL effector DNA binding domains. The specific targeting is aided by simple "code" that matches with the diamino acid sequence (repeat-variable di-residue) in ∼33-35 amino acid conserved target sequence. The progress in gene editing tools and development of various methods for easy synthesis and assembly of TALENs, allows the efficient editing at multiple sites. There have been various examples of the success of TALENs like knockout of the CCR5 gene for HIV resistance in human cells (Mussolino et al., 2011); destruction of the bacterial blight disease susceptibility gene in rice (Li et al., 2012); disruption of the LDL receptor in swine (Carlson et al., 2012); replacement of a tyrosine hydroxylase gene via TALEN-enhanced homologous recombination in zebrafish (Xiao et al., 2013;Zu et al., 2013).

CRISPR-Cas in Understanding Interactions
Gene editing has been highly appreciated for their ability to change the desired DNA fragment using engineered nucleases often called as molecular scissors. Since it edits the product according to fitment of the process it has various applications in a diversity of areas. The CRISPR-Cas system has been evidenced as most efficient, easy and simple (Kanchiswamy et al., 2016). CRISPR-Cas system, also known as third-generation programmable nuclease has a major role in crop protection.
There are approximately 11 CRISPR-Cas systems have been reported. They can be distinguished into three types (Types I-III) which are further divided into 11 subtypes (Ma and Liu, 2016). Each type has its own specific Cas protein component which is named according to model organism.
Cas9 is a DNA endonuclease guided by RNA to target foreign DNA for inhibition (Figure 5) The guide RNAs (gRNAs) are derived from CRISPRs. CRISPRs consists of tandem arrays of a 30-40 bp short, direct repeat sequence which are separated by spacer sequences that matches the foreign sequence. Further transcription and processing of CRISPR produces mature CRISPR (cr)RNAs, the sequence flanked by signature CRISPR repeat tag at 5 and 3 end. The CRISPR (cr)RNAs form complex with Cas proteins to form a ribonucleoprotein (crRNP) that introduce cleavage in the DNA/RNA of the invader (Hale et al., 2012). One of the remarkable features of CRISPR is the specificity, that is aided by gRNA, that allows specific binding to target DNA and beauty of the system lies in the customized engineering of the gRNA. The specificity was enhanced by using double nickase and Cas9-nuclease fusion systems. Double nickase system allows binding of two gRNAs, both upstream as well as downstream preventing off target editing. This was further improved by using inactivated Cas9, i.e., without nuclease activity, fused with restriction enzymes. The nuclease activity of restriction enzyme only gets activated when both are in close proximity (Guilinger et al., 2014). The gene of interest can be inserted or deleted from the system with the help of CRISPR/Cas9 by introducing DSBs into a target site (Vanamee et al., 2001;Auer et al., 2014). Suitable expression construct is required for successful accomplishment of CRISPR-Cas sgRNA sequence(s), the codon-optimized variant of Cas9, strong promoters suitable to derive transcription of sgRNA and Cas9 (Raitskin and Patron, 2016). The importance of all these parameters was elucidated in a review by Schaeffer and Nakata (2015). With progress in computational techniques various computational tools like E-CRISP, CRISPR design tool, and CHOPCHOP have been developed that allow to identify the probable sequence of cleavage using input target sequences. Therefore, it helps to design gRNA (Hsu et al., 2013;Heigwer et al., 2014;Montague et al., 2014).
Once the target site is recognized by the gRNA, the nuclease Cas9 with the aid of its two domains RucV and HNH breaks the strand and generate blunt end DSB. Such DSB can be repaired by NHEJ that introduce mutation at the targeted site or by HDR, that may knock-in or replace the desired gene fragment at the target site using template DNA. There are various examples of gene editing utilized by different microbes (Table 2). Additionally, multiple editing in the same cell is possible using multiple gRNA that show various applications, like mutation in genes which are  functionally related to control complex traits Xie et al., 2015). In a study, expression of Cas9 and sgRNA genes in Arabidopsis and tobacco, caused a targeted cleavage of a nonfunctional GFP gene. Further mutation by NHEJ DNA repair led to the production of a strong green fluorescence in transforming leaf cells (Jiang et al., 2013(Jiang et al., , 2014. To enhance the expression of Cas9 in plants, codon optimization is often used strategically (Fauser et al., 2014). For the expression of Cas9, constitutive promoters of ubiquitin genes of rice, Arabidopsis, and maize can attain the desired requirement of gene editing in monocot and dicot plants.

Plant-Virus Interactions and Desired Trait Improvement
Earlier, the studies on trait improvement were based on plant breeding, somatic hybridization, and random mutagenesis, the process was tedious and time consuming. The trend of plant breeding was replaced by efficient and simple tools, i.e., CRISPR-Cas to introduce specific traits into the population. The effort was done to enhance the sensitivity toward the herbicide. The three oligonucleotides were targeted by CRISPR-Cas via A. tumefaciens. The transformation was done using single gRNA in a binary vector and successfully mutants were found to be sensitive to bentazon herbicide. A genome modification study was done for the first time in the maize utilizing TALENs and CRISPR-Cas and concluded that both the systems efficiently can be used for genome modification in maize (Liang et al., 2014). Similar studies were done in tobacco and it also suggested that CRISPR-Cas is an efficient genome modification tool . The studies were done to enhance the gene targeting and it was observed that virus mediated transformation showed a higher frequency than the traditional A. tumefaciens T-DNA (Xu et al., 2014). Baltes et al. (2014) reported such finding in Nicotiana tabacum by using Gemini virus replicons to enhance the gene targeting and also revealed the DNA sequence editing using Gemini virus replicons. There have been a number of strategies for multiple gene targeting using multiple gRNA in a single plasmid vector described by Raitskin and Patron (2016). The Cas9 are now recently used to control the pests. In a study, the Cas9 was used to control the population of Drosophila melanogaster. Engineered endonuclease-based drive systems have been used to drive mutations into populations of pest species leading directly or indirectly to reduce population sizes (Reid and O'Brochta, 2016).
In near future, it is expected that CRISPR-Cas will prove as a remarkable tool to engineer plants to eradicate problems associated with crops like low yields, nutritional content, and resistance from biotic and abiotic factors. The technique can also be utilized to prevent the plant diseases by inhibiting the virus interaction with the plant system ( Figure 5). The bacterial CRISPR-Cas could be used to inhibit the viral genetic material with the action of Cas9 as a nuclease thereby curtailing the establishment of viral infection in the plant (Ali et al., 2015;Baltes et al., 2015;Chaparro-Garcia et al., 2015;Ji et al., 2015). There are various examples where CRISPR-Cas system has proved to be successful in improving plant traits. In a rice plant, genetic modification was done in large chromosomal segments of sugar efflux transporter genes that resulted in 87-100% editing in T0 transgenic plants (Zhou et al., 2014). The gene function was first time revealed in the citrus fruit with the aid of CRISPR-Cas (Jia and Wang, 2014). CRISPR/Cas9 technology is most useful in woody plants that have long reproductive cycles, as they have the ability to acquire mutants in T0 generation (Fan et al., 2015;Tsai and Xue, 2015). Indeed, such results of gene editing empower the idea of the customized editing and desired expression in all living systems.
Certainly, successful development of the Cas9/sgRNA system for targeted gene modification and genome editing holds promise for boosting fundamental knowledge of plant biology as well as for designing crop plants with potential new agronomic, nutritional, and novel traits for the benefit of farmers and consumers.

CONCLUSION
Microbes play a fundamental role in diverse ecosystems through microbial interactions with other biotic and abiotic components of the ecosystem. Plant-microbe interactions play an important role in plant health and ecological sustainability. So, comprehension of these interactions is very crucial to improve plant health and ecological sustainability. Recently, microbial interaction prediction using computational biology has become an extensively used approach to inspect the plant-microbial interactions. In this review, different computational methods developed by the computational data has been summarized to understand plant-microbe interactions. Several systems biology tools such as FBA (flux balance analysis), CBM, and OptKnock has been described to understand the metabolic pathways involved in plant-microbe interactions. Furthermore, gene editing tools such as TALENs and CRISPER-Cas have been described to control the pathogen interactions with plants to obtain customized plants. A snapshot of gene editing tools has been described to obtain disease free customized plants. There should be a better understanding of signaling pathways and metabolic networks to have an understanding of plant-microbial interactions. A combinatorial approach of computational biology and genomic tools has proven supportive to understand the communication pathway and metabolic pathway and provides an alternative to regulate these pathways to get a beneficial effect on plants with ecological sustainability.

AUTHOR CONTRIBUTIONS
All authors listed, have made substantial, direct and intellectual contribution to the work, and approved it for publication.