TY - JOUR
AU - Zhang, Zhong-Wei
AU - Wu, Zi-Li
AU - Feng, Ling-Yang
AU - Dong, Li-Hua
AU - Song, An-Jun
AU - Yuan, Ming
AU - Chen, Yang-Er
AU - Zeng, Jian
AU - Chen, Guang-Deng
AU - Yuan, Shu
PY - 2016
M3 - Original Research
TI - Mg-Protoporphyrin IX Signals Enhance Plant’s Tolerance to Cold Stress
JO - Frontiers in Plant Science
UR - https://www.frontiersin.org/articles/10.3389/fpls.2016.01545
VL - 7
SN - 1664-462X
N2 - The relationship between Mg-protoporphyrin IX (Mg-Proto IX) signals and plant’s tolerance to cold stress is investigated. Arabidopsis seedlings grown for 3 weeks were pretreated with 2 mM glutamate (Glu) and 2 mM MgCl2 for 48 h at room temperature to induce Mg-Proto IX accumulation. Then cold stress was performed at 4°C for additional 72 h. Glu + MgCl2 pre-treatments alleviated the subsequent cold stress significantly by rising the leaf temperature through inducing Mg-Proto IX signals. The protective role of Glu + MgCl2 treatment was greatly compromised in the mutants of Mg-Proto IX synthesis, Mg-Proto IX signaling, and cyanide-resistant respiration. And the enhancement of cold-responsive gene expression was greatly compromised in the mutants of Mg-Proto IX synthesis, Mg-Proto IX signaling and ABA signaling, but not in the mutant of cyanide-resistant respiration. Cold stress promoted cyanide-resistant respiration and leaf total respiration exponentially, which could be further induced by the Glu + MgCl2 treatment. Mg-Proto IX signals also activate antioxidant enzymes and increase non-enzymatic antioxidants [glutathione but not ascorbic acid (AsA)] to maintain redox equilibrium during the cold stress.
ER -