AUTHOR=Sharma Anket , Thakur Sharad , Kumar Vinod , Kanwar Mukesh K. , Kesavan Anup K. , Thukral Ashwani K. , Bhardwaj Renu , Alam Pravej , Ahmad Parvaiz 

TITLE=Pre-sowing Seed Treatment with 24-Epibrassinolide Ameliorates Pesticide Stress in Brassica juncea L. through the Modulation of Stress Markers

JOURNAL=Frontiers in Plant Science

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2016.01569

DOI=10.3389/fpls.2016.01569

ISSN=1664-462X

ABSTRACT=<p>The present experiment was designed to assess the effects of seed soaking with 24-epibrassinolide (EBR) on the physiology of <italic>Brassica juncea</italic> L. seedlings grown under imidacloprid (IMI) toxicity. Application of EBR increased the length of seedlings, dry weight, and pigment contents, polyphenols, total phenols, and organic acids under IMI toxicity. The expression of genes coding key enzymes of pigment, phenols, polyphenols, and organic acid biosynthetic pathways was also studied including <italic>CHLASE</italic> (chlorophyllase), <italic>PSY</italic> (phytoene synthase), <italic>CHS</italic> (chalcone synthase) and <italic>PAL</italic> (phenylalanine ammonialyase), <italic>CS</italic> (citrate synthase), <italic>SUCLG1</italic> (succinyl Co-A ligase,), <italic>SDH</italic> (succinate dehydrogenase), <italic>FH</italic> (fumarate hydratase), <italic>MS</italic> (malate synthase). Multiple linear regression (MLR) analysis revealed that IMI application regressed negatively on seedling length, dry weight and total chlorophyll content. However, EBR seed treatment regressed positively on all the parameters studied. Moreover, interaction between IMI and EBR showed positive regression for growth parameters, content of pigments, total polyphenol, total phenol and malate, and expression of <italic>PSY</italic> and <italic>PAL</italic>. Negative interactions were noticed for the contents of fumarate, succinate and citrate, and expression of <italic>CHS</italic> and all genes studied related to organic acid metabolism. In conclusion, EBR enhanced the growth and contents of all studied metabolites by regulating the gene expression of <italic>B. juncea</italic> seedlings under IMI stress.</p>