Setaria: A Food Crop and Translational Research Model for C4 Grasses

It is increasingly evident that new environmentally sustainable agricultural practices will be necessary to support an estimated human population of 9 billion by 2050. Considering the predicted climate change-associated events such as flooding, drought, increased temperatures, and increased soil salinity, it is critical to broaden our study and use of cereals and small grains. Millets offer significant potential toward meeting these goals. The genus Setaria comprises more than one hundred species distributed in a wide range of habitats in Asia, Africa, South America, and North America. Millets, including foxtail millet (Setaria italica), pearl millet (Pennisetum glaucum) and proso millet (Panicum milaceum), are consumed by millions of humans across the world, as well as used as forage and bird feed. Recently, foxtail millet and its wild-progenitor, green foxtail millet (S. viridis), have emerged as model plant systems for studying C 4 grass biology and other agronomic traits (Doust et al. Here, we comment on the significance of S. italica and S. viridis as model systems for agronomic and translational research related to improvement of nutritional quality, biotic and abiotic stress responses, photosynthetic efficiency and biomass potential of C 4 grasses. Foxtail millet is in the Paniceae tribe (sub-family Panicoideae of the Poaceae), which also contains pearl millet and proso millet. Foxtail millet was domesticated from green foxtail in northern China ca. 8000 years ago (Barton et al., 2009), and has been widely cultivated in arid and semi-arid regions of Asia, Africa and the Americas (Lata et al., 2013). It is one of the most resilient cereal crops, with good yields in dry and marginal land with minimal agricultural inputs. Foxtail millet also adapts well to adverse weather conditions such as low and unpredictable precipitation Because global climate change will have significant adverse effects on the production of other major cereals, foxtail-, pearl-and proso-millets are increasingly attractive alternatives for small grain production (Tadele, 2016). Green foxtail millet has also come into the spotlight in recent years as an attractive alternative to more established grass model systems such as rice and maize (Figure 1). S. viridis offers several research advantages, including short stature (10–30 cm), rapid life cycle (6–9 weeks), prolific seed production (∼13,000 seeds per plant), self-compatibility, small genome size (∼395 Mb), diploid genetics (2n = 18), ability to be grown in controlled environments under relatively low light levels, and amenability to transformation (Devos et al. Like maize, green and foxtail millets …

It is increasingly evident that new environmentally sustainable agricultural practices will be necessary to support an estimated human population of 9 billion by 2050. Considering the predicted climate change-associated events such as flooding, drought, increased temperatures, and increased soil salinity, it is critical to broaden our study and use of cereals and small grains. Millets offer significant potential toward meeting these goals. The genus Setaria comprises more than one hundred species distributed in a wide range of habitats in Asia, Africa, South America, and North America. Millets, including foxtail millet (Setaria italica), pearl millet (Pennisetum glaucum) and proso millet (Panicum milaceum), are consumed by millions of humans across the world, as well as used as forage and bird feed. Recently, foxtail millet and its wild-progenitor, green foxtail millet (S. viridis), have emerged as model plant systems for studying C 4 grass biology and other agronomic traits (Doust et al., 2009;Li and Brutnell, 2011;Lata et al., 2013;Mandadi et al., 2014;Xianmin et al., 2014;Brutnell, 2015;Brutnell et al., 2015). Here, we comment on the significance of S. italica and S. viridis as model systems for agronomic and translational research related to improvement of nutritional quality, biotic and abiotic stress responses, photosynthetic efficiency and biomass potential of C 4 grasses.
Foxtail millet is in the Paniceae tribe (sub-family Panicoideae of the Poaceae), which also contains pearl millet and proso millet. Foxtail millet was domesticated from green foxtail in northern China ca. 8000 years ago (Barton et al., 2009), and has been widely cultivated in arid and semi-arid regions of Asia, Africa and the Americas (Lata et al., 2013). It is one of the most resilient cereal crops, with good yields in dry and marginal land with minimal agricultural inputs. Foxtail millet also adapts well to adverse weather conditions such as low and unpredictable precipitation (Lata et al., 2013;. Furthermore, it appears to be resilient to multiple abiotic and biotic stresses such as drought (Zhang et al., 2005;Meng et al., 2009;Lata et al., 2011), salinity (Ardie et al., 2015) and fungal diseases (Xu et al., 2011). Because global climate change will have significant adverse effects on the production of other major cereals, foxtail-, pearland proso-millets are increasingly attractive alternatives for small grain production (Tadele, 2016).
Green foxtail millet has also come into the spotlight in recent years as an attractive alternative to more established grass model systems such as rice and maize (Figure 1). S. viridis offers several research advantages, including short stature (10-30 cm), rapid life cycle (6-9 weeks), prolific seed production (∼13,000 seeds per plant), self-compatibility, small genome size (∼395 Mb), diploid genetics (2n = 18), ability to be grown in controlled environments under relatively low light levels, and amenability to transformation (Devos et al., 1998;Doust et al., 2009;Brutnell et al., 2010;Li and Brutnell, 2011;Wang et al., 2011;Xianmin et al., 2014;Saha and Blumwald, 2016). Like maize, green and foxtail millets are C 4 plants, but they have smaller genomes and are true diploids. In combination with sorghum (Sorghum bicolor), S. italica and S. viridis are proving valuable in studying C 4 photosynthesis, with the long-term goal of engineering C 4 traits into important C 3 crops such as rice and wheat.
The S. italica and S. viridis system offer many genetic resources, including sequenced draft genomes (Bennetzen et al., 2012;Zhang et al., 2012) (Setaria viridis v1.1, DOE-JGI, http://phytozome.jgi.doe.gov) and a high-density haplotype map of genome variation (Jia et al., 2013). Comparisons of the arrangement of the nine chromosomes of S. italica and S. viridis with the corresponding chromosomes of rice and sorghum reveal relatively few rearrangements, and the genome sequence has been used to guide the assembly of the polyploid genome of its close relative, Panicum virgatum (switchgrass), a promising bioenergy feedstock (Daverdin et al., 2015). The high-quality genome sequence of foxtail millet has also allowed in-depth analyses of transposon family dynamics and locations that reveal hitherto unsuspected variation between transposon families in insertion site preference and turnover (Bennetzen et al., 2016). Mutant populations have been characterized in both foxtail millet and green foxtail millet, and the identity of candidate genes was revealed by novel high-throughput sequencing approaches (Li et al., 2016;Liu et al., 2016;Martins et al., 2016;Xue et al., 2016). S. italica and S. viridis have been used in the characterization of important agronomic traits, including yield-related architectural traits such as height, branching, biomass, flowering time and domestication-related traits such as shattering (Qian et al., 2012;Jia et al., 2013;Mauro-Herrera et al., 2013;Wang et al., 2013;Doust et al., 2014;Gupta et al., 2014;Layton and Kellogg, 2014;Qie et al., 2014;Fahlgren et al., 2015;Fang et al., 2016;Hodge and Kellogg, 2016;Liu et al., 2016;Mauro-Herrera and Doust, 2016).
In plants, salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) are key defense hormones that mediate signaling during plant-microbe interaction. Prevailing evidence, primarily based on studies of dicot plants, suggest that SA and JA/ET pathways are antagonistic to each other during plant-microbe interactions. Recently, in conjunction with Brachypodium distachyon (a C 3 grass), S. viridis was used to characterize the dynamics of C 3 vs. C 4 plant defense signaling responses against a diverse group of grass-infecting viruses (Scholthof, 1999;Mandadi and Scholthof, 2012;Mandadi et al., 2014). This study showed that SA and JA/ET crosstalk does exist in S. viridis during grass-virus interactions; however, there were also unique C 4 host-dependent signaling responses. S. italica was also used to dissect grass defense signaling pathways modulated during an incompatible interaction with a fungal pathogen, Uromyces setariae-italicae (Li et al., 2015). Together, these studies demonstrate the utility of S. italica and S. viridis for fundamental research pertaining to plant-microbe interactions.
S. italica and S. viridis are also very useful for translational research related to bioenergy traits. In addition to plantderived sugars it has been shown that cell-wall derived cellulose, hemicellulose and lignin are sustainable sources for bioenergy production. Owing to their superior carbon-assimilation and photosynthetic pathways, several C 4 grasses such as switchgrass, Miscanthus, and energycane have emerged as feedstocks for plant ligno-cellulosic biomass (Brutnell et al., 2010). Efforts are underway to engineer C 4 photosynthetic pathways into C 3 crops (e.g., rice) to improve photosynthetic capacity and yield (von Caemmerer et al., 2012;Karki et al., 2013). However, the genomes and genetics of the leading bioenergy grasses are complex, and are a hindrance in deciphering the mechanisms of C 4 photosynthesis, and ultimately to engineer them into C 3 grasses. Both S. italica and S. viridis are C 4 grasses and their genomes are highly syntenic to other Panicoid grasses (Kumari et al., 2013) and, in this context, these model plants offer powerful tools to understand the genetics of biomass production of panicoid bioenergy grasses, as well as to limn the evolution of C 4 vs. C 3 traits (Doust et al., 2009;Li and Brutnell, 2011;Lata et al., 2013;Mandadi et al., 2014;Brutnell et al., 2015;Martin et al., 2016).
In addition to enabling research into agronomic traits, S. italica and S. viridis are valuable models in the study of phytonutrient pathways pertaining to small grain millets. For example, S. italica is a good source of calories and essential micronutrients, and has higher nutritive value than major cereal grains like rice, wheat and sorghum. S. italica also contains high levels of proteins, dietary fibers, vitamins, anti-oxidants and non-starchy polysaccharides with low glycemic index, when compared to rice, wheat and sorghum (Taylor et al., 2006;Suma and Urooj, 2012;Amadou et al., 2013;. Moreover, over fifty phenolic compounds belonging to classes such as hydroxybenzoic acids, hydroxycinnamic acids and flavonoids were identified from S. italica (Chandrasekara and Shahidi, 2011b). In in vitro studies, phenolic compounds derived from S. italica, exhibited health promoting properties including antioxidant and free radical quenching, which helps in boosting immunity and inhibiting cancer cell proliferation (Dykes and Rooney, 2006;Chandrasekara and Shahidi, 2010, 2011a,b, 2012. Notwithstanding these reported nutritional qualities, certain anti-nutritional constraints were also reported for S. italica. These include the presence of toxic substances, some of which might be harmful to human health. For instance, some foxtail millet grains contain goitrogens that suppress thyroid activity which may have a role in goiter-an enlargement of the thyroid gland (Gaitan et al., 1989;Gluchoff-Fiasson et al., 1989;Elnour et al., 2000). The major goitrogenic and antithyroid compounds isolated were C-glycosylflavones (Gaitan et al., 1989;Gluchoff-Fiasson et al., 1989). Furthermore, in vivo experiments with vitexin (one of the three major Cglycosylflavones) in rats revealed evidence of antithyroid activity (Gaitan et al., 1995). In this context, further research is needed to carefully delineate the nutritional and anti-nutritional properties of phytochemicals present in S. italica and perhaps other small grain millets. The availability of S. italica and S. viridis genomic and genetic resources should enable research into identifying and inactivating such anti-nutritional compounds using novel genome-editing technologies (e.g., CRISPR-CAS9) to enhance S. italica nutritional traits.
Given the importance of S. italica as a food crop and in the study of grass biology, we suggest that S. italica, and its wildprogenitor, S. viridis, are promising translational research models to study nutritional pathways, abiotic and biotic stress resistance pathways, as well as to advance C 4 photosynthesis and bioenergy research.

AUTHOR CONTRIBUTIONS
SP, SI, AD, KS, and KM contributed to the design, preparation, and editing of the manuscript.