TY - JOUR AU - Cao, Xibing AU - Fan, Guoqiang AU - Dong, Yanpeng AU - Zhao, Zhenli AU - Deng, Minjie AU - Wang, Zhe AU - Liu, Wenshan PY - 2017 M3 - Original Research TI - Proteome Profiling of Paulownia Seedlings Infected with Phytoplasma JO - Frontiers in Plant Science UR - https://www.frontiersin.org/articles/10.3389/fpls.2017.00342 VL - 8 SN - 1664-462X N2 - Phytoplasma is an insect-transmitted pathogen that causes witches' broom disease in many plants. Paulownia witches' broom is one of the most destructive diseases threatening Paulownia production. The molecular mechanisms associated with this disease have been investigated by transcriptome sequencing, but changes in protein abundance have not been investigated with isobaric tags for relative and absolute quantitation. Previous results have shown that methyl methane sulfonate (MMS) can help Paulownia seedlings recover from the symptoms of witches' broom and reinstate a healthy morphology. In this study, a transcriptomic-assisted proteomic technique was used to analyze the protein changes in phytoplasma-infected Paulownia tomentosa seedlings, phytoplasma-infected seedlings treated with 20 and 60 mg·L−1 MMS, and healthy seedlings. A total of 2,051 proteins were obtained, 879 of which were found to be differentially abundant in pairwise comparisons between the sample groups. Among the differentially abundant proteins, 43 were related to Paulownia witches' broom disease and many of them were annotated to be involved in photosynthesis, expression of dwarf symptom, energy production, and cell signal pathways. ER -