%A Jha,Ambuj B. %A Gali,Krishna K. %A Tar’an,Bunyamin %A Warkentin,Thomas D. %D 2017 %J Frontiers in Plant Science %C %F %G English %K Ascochyta blight,genotyping-by-sequencing,Heterogeneous inbred family,Quantitative Trait Loci,Pisum fulvum,Psium sativum %Q %R 10.3389/fpls.2017.00765 %W %L %M %P %7 %8 2017-May-09 %9 Original Research %+ Thomas D. Warkentin,Crop Development Centre – Department of Plant Sciences, University of Saskatchewan, Saskatoon,SK, Canada,tom.warkentin@usask.ca %# %! Fine Mapping Ascochyta Resistance in Pea %* %< %T Fine Mapping of QTLs for Ascochyta Blight Resistance in Pea Using Heterogeneous Inbred Families %U https://www.frontiersin.org/articles/10.3389/fpls.2017.00765 %V 8 %0 JOURNAL ARTICLE %@ 1664-462X %X Ascochyta blight (AB) is an important disease of pea which can cause severe grain yield loss under wet conditions. In our previous study, we identified two quantitative trait loci (QTLs) abIII-1 and abI-IV-2 for AB resistance and these QTLs were consistent across locations and/or years in an inter-specific pea population (PR-19) developed from a cross between Alfetta (Pisum sativum) and P651 (P. fulvum). The objectives of this study were to fine map the abIII-1 and abI-IV-2 QTLs using a high density single nucleotide polymorphism (SNP)-based genetic linkage map and analyze identified markers in heterogeneous inbred family (HIF) populations. Selective genotyping of 51 PR-19 recombinant inbred lines was performed using genotyping-by-sequencing (GBS) and the resulting high density genetic linkage map was used to identify eight new SNP markers within the abI-IV-2 QTL, whereas no additional SNPs were identified within the abIII-1 QTL. Two HIF populations HIF-224 (143 lines) and HIF-173 (126 lines) were developed from F6 RILs PR-19-224 and PR-19-173, respectively. The HIF populations evaluated under field conditions in 2015 and 2016 showed a wide range of variation for reaction to AB resistance. Lodging score had significant positive (P < 0.001) correlation with AB scores. HIFs were genotyped using SNP markers within targeted QTLs. The genotypic and phenotypic data of the HIFs were used to identify two new QTLs, abI-IV-2.1 and abI-IV-2.2 for AB resistance within the abI-IV-2 QTL. These QTLs individually explained 5.5 to 14% of the total phenotypic variation. Resistance to lodging was also associated with these two QTLs. Identified SNP markers will be useful in marker assisted selection for development of pea cultivars with improved AB resistance.