Conserved Function of Fibrillin5 in the Plastoquinone-9 Biosynthetic Pathway in Arabidopsis and Rice

Plastoquinone-9 (PQ-9) is essential for plant growth and development. Recently, we found that fibrillin5 (FBN5), a plastid lipid binding protein, is an essential structural component of the PQ-9 biosynthetic pathway in Arabidopsis. To investigate the functional conservation of FBN5 in monocots and eudicots, we identified OsFBN5, the Arabidopsis FBN5 (AtFBN5) ortholog in rice (Oryza sativa). Homozygous Osfbn5-1 and Osfbn5-2 Tos17 insertion null mutants were smaller than wild type (WT) plants when grown on Murashige and Skoog (MS) medium and died quickly when transplanted to soil in a greenhouse. They accumulated significantly less PQ-9 than WT plants, whereas chlorophyll and carotenoid contents were only mildly affected. The reduced PQ-9 content of the mutants was consistent with their lower maximum photosynthetic efficiency, especially under high light. Overexpression of OsFBN5 complemented the seedling lethal phenotype of the Arabidopsis fbn5-1 mutant and restored PQ-9 and PC-8 (plastochromanol-8) to levels comparable to those in WT Arabidopsis plants. Protein interaction experiments in yeast and mesophyll cells confirmed that OsFBN5 interacts with the rice solanesyl diphosphate synthase OsSPS2 and also with Arabidopsis AtSPS1 and AtSPS2. Our data thus indicate that OsFBN5 is the functional equivalent of AtFBN5 and also suggest that the SPSs–FBN5 complex for synthesis of the solanesyl diphosphate tail in PQ-9 is well conserved in Arabidopsis and rice.

Fibrillins (FBNs), which are lipid-associated proteins, are found in all organisms performing oxygenic photosynthesis (Pozueta-Romerso et al., 1997;Kessler et al., 1999;Simkin et al., 2007;Cunningham et al., 2010;Heinnickel and Grossman, 2013;Lohscheider and Bártulos, 2016). In higher plants, FBN can be distinguished into 11 subfamilies (Singh and McNellis, 2011). New FBNs were recently identified in algae by searching FBN sequences from publicly available algal genomes (Lohscheider and Bártulos, 2016). Proteomic studies of plastid subcompartments have identified 12 known FBNs in Arabidopsis. Seven FBNs are strongly enriched in plastoglobules (PGs), while other FBNs are distributed throughout the stroma or are associated with the thylakoid membranes (Lundquist et al., 2012). Singh and McNellis (2011) showed that FBNs contain a conserved lipocalin "motif 1" in the N-terminal region and conserved residues in the C-terminal region, including aspartic acid. FBNs have been predicted to adopt a threedimensional β-barrel structure with a small α-helical lid, similar to that of lipocalin (Lohscheider and Bártulos, 2016). This structure indicates that FBNs are involved in the binding and transport of small hydrophobic molecules (Flower, 1996;Singh and McNellis, 2011).
FBNs are mainly found in chromoplasts, PGs, and algal eyespots and have important roles in the formation of fibril structures in these organelles (Duruère et al., 1994;Rey et al., 2000;Singh et al., 2010). Group 1 members have been reported to be involved in PG formation and thylakoid maintenance (Rey et al., 2000;Simkin et al., 2007). Group 4 members have also been shown to be involved in PG formation. Furthermore, FBNs are involved in growth and development, tolerance to oxidative stress, and hormone signaling (Leitner-Dagan et al., 2006;Singh et al., 2010). The cyanobacterium Synechocystis pgl1/pgl2 mutant exhibits altered thylakoid ultrastructure, reduced pigment levels, and is more susceptible to light (Cunningham et al., 2010). Group 1 and 2 members are involved in hormone signaling such as abscisic acid-mediated, jasmonate-mediated, and gibberellinmediated responses to abiotic stress (Yang et al., 2006;Youssef et al., 2009). FBN4 has been suggested to be involved in the transport of PQ-9 between thylakoids and PG, an idea supported by the finding that FBN4 deficiency in apple and Arabidopsis plants resulted in reduced tolerance to abiotic and biotic stresses (Singh et al., 2010(Singh et al., , 2012. In addition, we recently showed that FBN5 is involved in PQ-9 biosynthesis in Arabidopsis. Arabidopsis mutant plants containing low levels of FBN5-B accumulated less PQ-9 and PC-8, leading to their increased susceptibility to cold stress and lower photosynthetic performance. FBN5-B interacted with SPS1 and SPS2 in chloroplasts. It was hypothesized that FBN5-B stimulates the enzymatic activity of SPS1 and SPS2 by binding to the hydrophobic solanesyl moiety and helping to release this moiety from the enzymes in Arabidopsis (Kim et al., 2015).
Although FBNs are presumed to play significant roles in photosynthetic organisms, they have been identified and characterized in only a few plant species to date (e.g., Arabidopsis, cucumber, tomato, and pepper) (Singh and McNellis, 2011). Rice is a major cereal. Thus, characterization of FBNs function in rice would increase our knowledge about maintaining photosynthetic efficiency and stress tolerance. In this study, we identified an Arabidopsis FBN5 (AtFBN5) ortholog, OsFBN5, in rice and investigated its function in two rice Osfbn5 Tos17 insertion mutants. We found that deficiency of OsFBN5 resulted in reduced levels of PQ-9 and PC-8 in the leaves and increased susceptibility to excess light energy. Moreover, OsFBN5 interacted with AtSPS1, AtSPS2, and OsSPS2. OsFBN5 was also capable of complementing AtFBN5 function when expressed in the fbn5-1 Arabidopsis mutant. The contents of PQ-9 and PC-8 present in complemented mutants were almost indistinguishable from those in WT Arabidopsis plants. These results provide strong evidence that the function of FBN5 in PQ-9 biosynthesis is well conserved between eudicots and monocots.

Plant Materials and Growth Conditions
The Arabidopsis thaliana Columbia-0 ecotype (WT) and the AtFBN5/fbn5 T-DNA insertion mutant (Salk_064597), the latter of which harbors a disruption in FBN5 (At5g09820), were grown in soil or agar plates containing 0.5× MS (Murashige and Skoog) medium supplemented with/without sucrose. Plants were propagated under a 16 h light/8 h dark photoperiod with 100 µmol m −2 s −1 fluorescence light at 22 • C. Two rice Tos17 insertion mutant alleles, Osfbn5-1 (ND8652) and Osfbn5-2 (NG2517) (cultivar Nipponbare), were isolated from the Rice Tos17 Insertion Mutant Database 1 . Rice seeds were germinated on 0.5× MS agar medium supplemented with 3% sucrose. The plants were grown at 28 • C for 7 days under 100 µmol m −2 s −1 light intensity. Uniformly grown seedlings were transferred into fresh water or transplanted onto soil in the growth chamber and grown with 100 or 600 µmol m −2 s −1 light at 28 • C.

PCR Analysis
The genotypes of the Osfbn5 mutant alleles, the Arabidopsis fbn5-1 (Atfbn5-1) mutant, and the complemented lines were determined by genomic DNA PCR analysis with a genotyping 1 https://tos.nias.affrc.go.jp/ primer set (Supplementary Table 1). Total RNA was extracted from the leaf tissues using an RNeasy kit (Qiagen), which included a DNase treatment step. cDNA was synthesized from total RNA using RNA to cDNA EcoDry TM premix (Clontech, CA, United States) following the manufacturer's instructions. The PCR reactions were performed with Takara Ex Taq DNA polymerase and an RT-PCR primer set (Supplementary Table 1).

Analysis of Prenyl-Lipids
To analyze the PQ-9, PC-8, tocopherol, carotenoid, and chlorophyll contents of the plants, total lipids were extracted and analyzed with HPLC using a Shimadzu LC-20AD chromatography unit as previously described (Kim et al., 2015). Chromatography was conducted at 30 • C on a C18 reverse-phase column (5 µM Supelco Discovery C18 column, 250 × 4.6 mm). Total lipids were extracted from frozen tissue grindates in liquid nitrogen in cold ethyl acetate. After centrifugation, the supernatant was transferred to a new tube and evaporated under nitrogen. The extract was redissolved in 95% ethanol. PQ-9, PC-8, and tocopherols were analyzed with an isocratic solvent system consisting of methanol/hexane (9:1, v/v) at a flow rate of 1.0 mL min −1 . PQ-9 was detected by absorption at 255 nm, while PC-8 and tocopherols were detected fluorimetrically (290 nm excitation and 330 nm emission). The compounds were quantified by comparison to their corresponding external calibration standards, and the data were corrected by comparison with the recovery of rac-Tocol (Matreya, PA, United States) as the internal standard.
For the analysis of carotenoids and chlorophylls, the chromatographic conditions were the same as above. Solvent A (acetonitrile:water = 9:1 v/v with 0.1% triethylamine) and solvent B (ethyl acetate) were used with the following gradient: 0 to 5 min, 0 to 33.3% B; 5 to 33 min, 33.3 to 66.7% B; 33 to 33.5 min, 66.7 to 100% B; 33.5 to 38 min, 100% B; 38 to 38.5 min, 0% B; and 38.5 to 43 min, 0% B. The HPLC peak areas at 440 nm were integrated.

Yeast Two-Hybrid Assay
The coding sequences for OsFBN5 and OsSPS2 without the predicted chloroplast transit peptide regions were amplified from rice leaf cDNA with the yeast two-hybrid primer set (Supplementary Table 1). The amplified products were cloned into the entry vector pENTR TM /D-TOPO R (Invitrogen, CA, United States) and then subcloned into two destination vectors, pDEST-GBKT7 (bait) and pDEST-GADT7 (prey) using the Gateway system. The resulting bait and prey vectors, together with the AtFBN5, AtSPS1, and AtSPS2 vectors (Kim et al., 2015), were introduced into the yeast strains PBN204 (containing URA3, ADE2, and lacZ as reporters) and AH109 (containing HIS3, ADE2, and lacZ). Transformants were spotted onto synthetic defined medium lacking Leu and Trp (SD-LW) or also lacking Ura (SD-LWU), Ade (SD-LWA), or Ade and His (SD-LWAH). After incubation for 3 days at 30 • C, the colonies were replica-plated onto several selective media. As a negative control, cells transformed with parental bait (pGBKT7) and prey (pGADT7) vectors (Clontech, CA, United States) were used. Cells transformed with the SV40 large T-antigen (pGBKT7) and murine p53 (pGADT7) vectors were used as a positive control.

BiFC Assay
The coding sequences for OsFBN5 and OsSPS2 without stop codons were amplified with the bimolecular fluorescence complementation (BiFC) primer set (Supplementary Table 1). The amplified products were cloned into pENTR TM /D-TOPO R (Invitrogen, CA, United States) and then inserted into the pJJ2536 (containing the N-terminus of YFP) and pJJ2537 (containing the C-terminus of YFP) destination vectors for the BiFC assay. To assay transient expression, maize mesophyll protoplasts (5 × 10 5 cells per sample) were isolated from the second leaves of etiolated plants. The vectors were delivered into maize mesophyll protoplasts using the polyethylene glycolcalcium mediated method, followed by 12-16 h incubation to allow transient expression (Cho et al., 2009). Chlorophyll autofluorescence was used as a chloroplast marker. Expression of the fusion constructs was monitored using a confocal microscope (LSM 510 META, Carl Zeiss).

Complementation of Atfbn5-1 with OsFBN5
To produce the complementation constructs, the full coding sequence of OsFBN5 was amplified with the complementation primer pairs (Supplementary Table 1). The amplicon was cloned into the entry vector pENTR TM /D-TOPO R and then subcloned into the destination vector pB2GW7 (Karimi et al., 2002) using the Gateway system. The resulting construct was transformed into heterozygous FBN5/fbn5-1 Arabidopsis plants by Agrobacterium (GV3101) mediation using the floral dip transformation method (Clough and Bent, 1998). Transformants were selected via their resistance to BASTA (Bayer). Genomic DNA was extracted from the leaves of the BASTA-resistant plants and used to identify the homozygous fbn5-1 plants harboring 35S:OsFBN5 via PCR. OsFBN5 expression was detected in the complemented fbn5-1 plants using the appropriate primer pairs (Supplementary Table 1). Actin2 was used as an internal control.

Measurement of Photosynthetic Parameters
A portable chlorophyll fluorimeter (Walz) was used to measure the maximal photochemical activity of PSII (F v /F m ) under atmospheric conditions. Prior to F v /F m measurement, leaves were adapted to the dark for 10 min. F v /F m was calculated as (F m − F o )/F m , where F o is the initial chlorophyll fluorescence level, and F m is the maximal fluorescence level, determined with an intense pulse of white light. The stress-dependent reduction in F v /F m was interpreted as the photoinhibition of PSII.

Phylogenetic Analysis
FBN orthologs in rice were identified by similarity searches using BLAST 2 and phytozome v10.1 3 . The protein sequences of the individual FBN family members from Arabidopsis were used as queries against the rice genome. Sequence alignment was performed using the default ClustalW parameters and maximum likelihood was used for tree construction in MEGA6 program.

Identification of FBN5 Homologs from Rice
Previously, we proposed a novel function for FBN5 in the PQ-9 biosynthetic pathway of Arabidopsis (Kim et al., 2015). To investigate the roles of FBNs in rice, a monocot model species, a phylogenetic tree was constructed based on amino acid sequences FIGURE 2 | Isolation and growth phenotypes of the OsFBN5 mutant alleles. (A) Schematic diagram of the rice FBN5 gene with the Tos17 insertion. Black boxes and black lines represent exons and introns, respectively. The Tos17 insertion sites for two independent mutant lines, Osfbn5-1 and Osfbn5-2, are indicated by red lines. Arrows with names indicate primers for PCR experiments. (B) Genomic DNA PCR analysis of Osfbn5-1 and Osfbn5-2 progeny plants. A 1.1-kb PCR product was detected using the LP+RP primers, but not the LTR1+RP primers, in WT segregants. In knockout mutants, 830 bp and 850 bp products were detected using the LTR1+LP primers, but not the LP+RP primers. A representative sample from each indicated genotype is shown. (C) RT-PCR analysis of OsFBN5 and OsSPS2 expression in segregant wild type (WT), Osfbn5-1, and Osfbn5-2 mutant lines. The OsFBN5 transcript was amplified by primers P1+P2. A product was detected in WT segregants, but not in Osfbn5-1 or Osfbn5-2 plants. OsUBQ5 was used as a PCR loading control. (D) Photographs of WT, Osfbn5-1, and Osfbn5-2 seedling plants at 1, 3, and 5 days after transferring 7-day-old plants into water. (E) Plant height and (F) fresh weight were measured on the 5th day in water. Each data point represents the mean (± SD) of five different plants. The asterisk indicates a significant difference between WT and mutant plants ( * P < 0.05; Student's t-test).
Frontiers in Plant Science | www.frontiersin.org of FBNs from Arabidopsis and rice. Arabidopsis and rice express 14 and 11 FBN genes, respectively (Supplementary Figure 1). Analysis of putative physicochemical properties, isoelectric points (PIs), and hydrophobicities of the Arabidopsis and rice FBN sequences after removal of the predicted plastid targeting peptides revealed predicted similar physicochemical properties between the Arabidopsis and rice FBN homologs (Supplementary Figure 2). Each FBN Arabidopsis/rice homolog pair showed more similar PIs than hydrophobicities. While the FBN1, FBN3, and FBN7 genes are duplicated in Arabidopsis, each of these genes is unique in rice. AtFBN5 and OsFBN5 shared 60% amino acid identity after removal of the predicted plastid targeting peptides (Figure 1). In Arabidopsis, an alternatively spliced transcript of FBN5, AtFBN5-A, could not interact with SPS (Kim et al., 2015). The product of this transcript was altered in 14 amino acids, including a 10 amino acid deletion at the C-terminus, indicating that these residues are indispensable for AtFBN5 function. These residues are well conserved in OsFBN5 (Figure 1), suggesting that OsFBN5 can bind SPS. A fairly well conserved lipocalin motif 1 was present in the Arabidopsis (DKIGGCWKLIY) and rice (DKVDGCWRLVY) FBN5s. Aspartic acid residues in the C-terminal regions, which are highly conserved in the FBN family, are present in Arabidopsis and rice FBNs (Singh and McNellis, 2011). The high amino acid sequence similarity of OsFBN5 and AtFBN5 suggests that OsFBN5 might function as a structural protein providing a scaffold for prenyl chain assembly during the synthesis of PQ-9 in rice, similar to Arabidopsis AtFBN5.

Isolation and Characterization of FBN5 Knockout Rice Mutants
We identified two Tos17 insertion mutant alleles in the sixth exon of OsFBN5 (LOC_Os04g34460), Osfbn5-1 and Osfbn5-2, and confirmed their insertions by genomic DNA sequencing (Figure 2A). Progeny seeds from the self-fertilized heterozygous plants, designated OsFBN5/Osfbn5-1 and OsFBN5/Osfbn5-2, were germinated on agar medium supplemented with 3% sucrose. Genotype analysis of growing seedlings by genomic DNA PCR with OsFBN5-specific and Tos17-specific primers revealed nearly normal segregation of Mendelian inheritance (Figure 2B and Supplementary Table 2). RT-PCR of RNA isolated from the selected homozygous plant showed that no OsFBN5 transcripts had accumulated (Figure 2C). Segregant WT and homozygote plants were grown on MS medium supplemented with 3% sucrose for one week and then transferred to water for growth observation. Unlike the Atfbn5-1 plants, the Osfbn5 homozygous mutant plants were not seedling lethal. However, they were smaller than the WT plants (Figures 2D-F). When the WT and Osfbn5 homozygous mutant plants were transplanted onto soil in the greenhouse, the mutant plants rapidly dried and eventually died, while the WT plants grew and yielded seeds.
The maximum efficiency of PSII photochemistry was measured by the chlorophyll fluorescence parameter OsFBN5 Mutation Reduces the Levels of PQ-9 and PC-8 Since AtFBN5 is involved in the PQ-9 biosynthetic pathway (Kim et al., 2015), we next used HPLC to determine the amounts of PQ-9, PC-8, tocochromanols, carotenoids, and chlorophyll in the mutant and WT plants. The amount of PQ-9 was reduced about sevenfold in the leaves of Osfbn5-1 and Osfbn5-2 mutant  Reversed-phase HPLC was used to analyze total lipids from the leaves 1 day after the 7-day-old plants were transferred into water. The quantities of individual components were determined relative to standards. Asterisks represent significance relative to WT. * P < 0.05, * * P < 0.01, and * * * P < 0.001 (Student's t-test). Data are means ± SD (n = 3-5). ND, not detected. Toc, tocopherol; Neo, neoxanthin; Vio, violaxanthin; Ant, antheraxanthin; Lut, lutein; β-Car, β-carotene; Car, carotene; Chl, chlorophyll. plants compared with the amount in WT plants (Figures 4A,C). PC-8, the product of PC-9 cyclization, was not detectable in either of the mutants, in contrast to WT plants (Figures 4A,C). In the Osfbn5-1 and Osfbn5-2 mutant plants, the levels of γ-tocopherol, α-tocopherol, and total tocopherols were not significantly different (Figures 4B,D). The level of β-carotene in the Osfbn5-1 plants was reduced by about 30% compared with that in the WT plants, whereas the levels of other carotenoids were not significantly reduced in Osfbn5-1 than WT plants ( Figure 4E). The level of antheraxanthin was increased in the Osfbn5-1 plants (Figure 4E), indicating that the mutant plants were more photostressed than the WT plants. Both chlorophyll a and b levels were slightly reduced in the Osfbn5-1 plants compared with the WT plants ( Figure 4F). FIGURE 5 | Yeast two-hybrid assay to test the strength of the interaction of OsFBN5 and AtFBN5 with SPS enzymes from rice and Arabidopsis. AH109 transformants were dropped onto selective medium lacking Leu and Trp (SD-LW) or also lacking Ade (SD-LWA) or Ade and His (SD-LWAH), with or without 5 mM 3-amino-1,2,3-triazole (3-AT), a competitive inhibitor of yeast HIS3. Positive control, yeast transformed with the P53 bait plasmid and the Tag prey plasmid. Negative control, yeast transformed with the parental bait vector (pGBKT7) and the prey vector (pGADT7).

OsFBN5 Interacts with SPSs from Rice and Arabidopsis
OsSPS2 has been shown to provide an SPP for PQ-9 formation in rice (Ohara et al., 2010). Therefore, we tested whether OsFBN5 interacts with OsSPS2 using the yeast two-hybrid system. After confirming that the fusion product of the mature OsFBN5 cDNA and the GAL4 DNA binding domain (BD fusion) did not retain transcriptional activation ability, this fusion protein was used as the bait. We also tested interactions of OsFBN5 and OsSPS2 with their Arabidopsis counterpart. OsFBN5 interacted with AtSPS1 and AtSPS2, as well as with OsSPS2 in both AH109 cells and PBN204 cells (Figure 5 and Supplementary Figure 3). In addition, AtFBN5 interacted with OsSPS2 in both yeast lines (Figure 5 and Supplementary Figure 3). We next tested the strength of the interaction between the proteins in AH109 cells on medium containing 3-amino-1,2,3-triazole, a competitive inhibitor of yeast HIS3. These experiments revealed that the interactions of OsFBN5 and AtFBN5 with OsSPS2, AtSPS1, and AtSPS2 were as strong as those of the positive control proteins (Figure 5). Yeast growth and β-galactosidase activity assays in PBN204 cells showed less strong interaction of OsFBN5 with OsSPS2 and AtSPS1 than with AtSPS2 (Supplementary Figure 3). OsSPS2 exhibits 77.5 and 76.6% identity with AtSPS1 and AtSPS2, respectively, after removal of the chloroplast targeting peptide (Supplementary Figure 4). Interestingly, the transcript levels of OsSPS2 were reduced in the Osfbn5 homozygous mutant plants than WT (Figure 2C). The OsFBN5-OsSPS2 interaction was further confirmed by BiFC experiments in maize mesophyll protoplasts. The YFP signals overlapped well with the chlorophyll autofluorescence signal (Figure 6), suggesting that these two proteins interact with each other in chloroplasts.

Complementation of the Atfbn5-1 Mutant with the Rice Ortholog of FBN5
To test the relevance of the OsFBN5 and AtSPSs interactions and to determine whether rice FBN5 is capable of functionally replacing AtFBN5, OsFBN5 cDNA was constitutively expressed under the CaMV 35S promoter in the fbn5-1 Arabidopsis mutant. Two Atfbn5-1 + 35S:OsFBN5 transgenic complemented lines (#1 and #2), each of which contained OsFBN5 cDNA and was FIGURE 6 | Visualization of the OsFBN5-OsSPS2 interaction in maize protoplasts by the BiFC assay. Maize mesophyll protoplasts were cotransfected with recombinant vectors containing the indicated constructs. Chlorophyll autofluorescence was used as a chloroplast marker. Fluorescence was observed 12-16 h after transfection.
homozygous for Atfbn5-1, were identified by genomic DNA PCR (Figures 7A,B). RT-PCR analysis showed that OsFBN5 was expressed in the Atfbn5-1 transgenic plants but not in their WT counterpart (Figure 7C). The Atfbn5-1 + 35S:OsFBN5 lines displayed a WT phenotype (Figure 7A), indicating that rice OsFBN5 in fbn5-1 Arabidopsis plants has a function similar to that of AtFBN5 in WT Arabidopsis plants. The levels of PQ-9 and PC-8 in the Atfbn5-1 + 35S:OsFBN5 lines, which were deficient in Atfbn5-1, were comparable to the levels in WT plants ( Figure 7D). Moreover, the levels of total tocopherols in the complemented Arabidopsis plants were similar to the levels in WT plants ( Figure 7E). The levels of γ-tocopherol in the complemented Arabidopsis plants, which were elevated in Atfbn5-1 plants, were recovered to those of WT plants. These results demonstrate that rice OsFBN5 is able to complement the Atfbn5-1 mutant plants, suggesting the similar function of OsFBN5 with AtFBN5 in Arabidopsis PQ-9 biosynthesis.

DISCUSSION
We have previously shown that a plastid lipid-associated protein, FBN5, is required for PQ-9 biosynthesis in Arabidopsis through physical and functional interactions with SPS (Kim et al., 2015). Our working hypothesis is that FBN5 binds to hydrophobic solanesyl moieties generated by SPS1 and SPS2 in FBN5/SPS homodimeric complexes and removes these moieties from the enzyme active sites to catalyze the reaction and maintain substrate turnover. In the present study, we proposed that FBN5 function in PQ-9 synthesis is not limited to Arabidopsis and is well conserved in rice, a monocot.
FBNs are present in all oxygenic photosynthetic organisms and were recently described as "green cut proteins, " referring to a group of proteins proposed to be necessary for optimal photosynthetic processes (Heinnickel and Grossman, 2013). Although FBNs appear to be involved in important processes in higher plants, their functions in rice are not known. Here, we show that the FBN family is well conserved in rice ( Supplementary Figure 1), and that the physiochemical properties of FBN family members are very similar to those of their Arabidopsis orthologs, especially PI (Supplementary Figure 2). The localization of each rice FBN protein is expected to be consistent with the PG, stromal, and thylakoid membrane localizations of the orthologous Arabidopsis FBN proteins identified in a previous proteomics study. Moreover, FBN localization in Arabidopsis is correlated with PI (Lundquist et al., 2012).
OsFBN5 physically interacts with OsSPS2 (Figures 5, 6), suggesting involvement of FBN5 in SPP moiety synthesis. Yeast growth and β-galactosidase activity assays in PBN204 cells suggested less strong interaction of OsFBN5 with OsSPS2 and AtSPS1 than with AtSPS2 (Supplementary Figure 3). However, this is likely attributed from other reasons such as lower expression of OsSPS2 and AtSPS1 in PBN204 cells rather than less strong interactions of them with OsFBN5. Because the yeast growth in AH109 cells indicated that the interactions of OsFBN5 with OsSPS2 and AtSPS1 were as strong as that of OsFBN5 and AtSPS2. In addition, the strength test of the interactions between the proteins in AH109 cells against a competitive inhibitor of yeast HIS3 showed similar strength between OsFBN5 with OsSPS2, AtSPS1 and AtSPS2 (Figure 5). The high degree of sequence conservation (over 60% identity) between Arabidopsis and rice FBN5 and SPS suggests that OsFBN5 is also able to physically interact with Arabidopsis SPS1 and SPS2, like AtFBN5 (Figure 5 and Supplementary Figure 4). The ability of rice FBN5 to complement the fbn5-1 mutation in Arabidopsis indicates that OsFBN5 interacts with AtSPS1 and AtSPS2, thus participating in PQ-9 synthesis in Arabidopsis chloroplasts (Figure 7).  Table 1) were detected in the #1 and #2 complemented lines but not in WT plants. The AtFBN5 transcript amplified by the F+R primers (Supplementary Table 1) was only detected in WT plants. AtACT2 was used as a PCR loading control. (D) PQ-9 and PC-8 contents and (E) tocopherol contents in leaves from WT plants, #1 and #2 complemented lines, and Atfbn5-1 plants. Contents were analyzed by reverse-phase HPLC. Asterisks represent significance relative to WT. * P < 0.05 and * * * P < 0.001 (student's t-test). Data are mean ± SD (n = 3-5). ND, not detected. Similar to Arabidopsis (Block et al., 2013), rice also expresses three SPS genes, OsSPS1 (LOC_Os06g46450), OsSPS2, and OsSPS3 (LOC_Os12g17320) (Supplementary Figures 4, 5). OsSPS1 and OsSPS2 have been shown to be responsible for ubiquinone-9 and PQ-9 SPP synthesis, respectively (Ohara et al., 2010;Ducluzeau et al., 2011). OsSPS3 is predicted to be targeted to chloroplasts, shares 90% identity with OsSPS2 ( Supplementary  Figure 4), and is phylogenetically closer to OsSPS2 than OsSPS1 (Block et al., 2013). Examination of OsSPS1, OsSPS2, and OsSPS3 expression data in different organs available in the Rice Genome Annotation Project public expression database 4 showed that OsSPS2 and OsSPS3 are highly expressed in leaves, while OsSPS1 is mainly expressed in embryos, pistils, and anthers (Supplementary Figure 5). Although OsSPS3 activity for SPP synthesis has not yet been reported, OsSPS3 is presumed to be a paralog of OsSPS2, raising the possibility that it can also contribute to PQ-9 biosynthesis. OsSPS2 might be a major contributor to PQ-9 biosynthetic flux since its transcript level was higher than that of OsSPS3 in leaf tissue (Supplementary Figure 5). Moreover, the high amino acid sequence similarity of OsSPS3 with AtSPS1 and AtSPS2 (Supplementary Figure 4) also suggests that OsSPS3 may interact with AtFBN5 and OsFBN5.
FBN functions appear to be conserved among plant species. For instance, Arabidopsis FBN4 mutants and FBN4 knockdown apple trees showed increased sensitivity to various oxidative stresses and alteration in the accumulation of osmophilic material inside PG, implying that FBN4 is involved in plant stress tolerance and trafficking of hydrophobic molecules between thylakoids and PGs (Singh et al., 2010). In addition, FBN group 1 genes are involved in disease resistance. Knockdown of LeChrC (FBN1) gene expression in tomato (Leitner-Dagan et al., 2006) and knockdown of FBN1b gene expression in Arabidopsis (Cooper et al., 2003) resulted in increased susceptibility to the fungus Botrytis cinerea and the bacterium Pseudomonas syringae pv. maculicola, respectively. Furthermore, silencing of the C40.4 (FBN1) gene in potato resulted in stunted growth, decreased tuber yield, and reduced non-photochemical quenching (Monte et al., 1999) and overexpression of bell pepper FBN1 resulted in increased plant height under stress conditions (Rey et al., 2000). These results suggest that FBN group 1 genes are related to modulation of photosynthetic efficiency and plant development upon stress.
We found that the Osfbn5 mutants displayed about sevenfold lower levels of PQ-9 compared to the WT plants and an undetectable amount of PC-8 (Figures 4A,C). Osfbn5 mutants exhibited slow growth and lower photosynthetic efficiency under normal growth conditions (Figures 2, 3), demonstrating that PQ-9 is required for plant growth and optimal photosynthetic performance. When Osfbn5 mutant plants were exposed to high light conditions, the PQ-9 defect was exacerbated, leading to photodamage of PSII and death (Figure 3). The PQ-9 deficiency in the Osfbn5 mutants is likely the cause of the reduced chlorophyll and carotenoid contents, but not the tocopherol contents (Figure 4). These features of Osfbn5 are consistent with previous studies performed with PQ-9-deficient plants such as Atfbn5-1, atsps1, atsps2, and atsps1atsps2 (Block et al., 2013;Kim et al., 2015). By monitoring the decay of radioactivity incorporated into PQ-9 from a labeled precursor, the half-life of PQ-9 was shown to be very short in spinach cells (Wanke et al., 2000). Furthermore, upon sudden exposure of Arabidopsis plants to excessively high light conditions, total PQ-9 contents were dramatically decreased. This effect was followed by a progressive increase, with almost fourfold enhanced accumulation compared to PQ levels measured before stress at the end of the time course (Ksas et al., 2015). Recent studies focused on PQ-9 antioxidant functions, thus expanding the PQ-9 role as a photosynthetic electron carrier between PSII and PSI and a redox gene regulator (Nowicka and Kruk, 2012;Ksas et al., 2015;Kruk et al., 2016). Therefore, the capacity of PQ-9 synthesis must be optimized in order to maintain stable yet dynamic PQ-9 concentrations to enable suitable physiological responses (Szymańska and Kruk, 2010), as supported by our OsFBN5 results.
Recently, Lohscheider and Bártulos (2016) reported the distribution and numbers of FBNs in some algae and higher plants that perform oxygenic photosynthesis. Interestingly, some algae belonging to Chlorophyta such as Ostreococcus tauri and Micromonas pusilla do not contain FBN5 orthologs, while Chlamydomonas reinhardtii does. We also identified a few plant species that do not contain FBN5 orthologs (data not shown). It would be interesting to determine whether these organisms have alternative structural proteins that function like FBN5 or whether their SPS enzymes contain a structural domain for binding to the SPP moiety for PQ-9 synthesis.