%A Fan,Xiangyun %A Zhu,Juan %A Dong,Wenbin %A Sun,Yuandong %A Lv,Chao %A Guo,Baojian %A Xu,Rugen %D 2017 %J Frontiers in Plant Science %C %F %G English %K barley,Amylopectin,QTL,GWAS,genotyping-by-sequencing,SSⅡa %Q %R 10.3389/fpls.2017.01531 %W %L %M %P %7 %8 2017-September-05 %9 Original Research %+ Rugen Xu,Jiangsu Key Laboratory of Crop Genetics and Physiology, Co-Innovation Center for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Barley Research Institution of Yangzhou University, Yangzhou University,Yangzhou, China,rgxu@yzu.edu.cn %# %! SSⅡa for Grain Amylopectin Content in Barley %* %< %T Comparative Mapping and Candidate Gene Analysis of SSIIa Associated with Grain Amylopectin Content in Barley (Hordeum vulgare L.) %U https://www.frontiersin.org/articles/10.3389/fpls.2017.01531 %V 8 %0 JOURNAL ARTICLE %@ 1664-462X %X Amylopectin concentration in barley endosperm has important effects on grain quality and end-use. In this study, quantitative trait locus (QTL) analysis together with genome-wide association studies (GWAS) were performed to identify markers linked to grain amylopectin content respectively using a doubled haploid (DH) population of 178 lines and a collection of 185 diverse barley germplasms both genotyped by genotyping-by-sequencing (GBS). A stable QTL on chromosome 7H and 11 associated single nucleotide polymorphisms (SNPs) were detected. In the co-localized region, the SSIIa (SSII-3) gene was predicted as the candidate gene. Then we isolated and characterized biparental SSIIa alleles of the DH population, investigated the expression pattern by quantitative real-time PCR (qRT-PCR), and revealed that a 33-bp deletion in exon 2 is responsible for reducing SSIIa transcript, thus resulting in a reduced amylopectin content. A sequence-based molecular marker was developed for the SSIIa allele and validated the effectivity, which would provide help for barley breeding.