TY - JOUR AU - Li, Xingwen AU - Sanagi, Miho AU - Lu, Yu AU - Nomura, Yuko AU - Stolze, Sara Christina AU - Yasuda, Shigetaka AU - Saijo, Yusuke AU - Schulze, Waltraud X. AU - Feil, Regina AU - Stitt, Mark AU - Lunn, John E. AU - Nakagami, Hirofumi AU - Sato, Takeo AU - Yamaguchi, Junji PY - 2020 M3 - Original Research TI - Protein Phosphorylation Dynamics Under Carbon/Nitrogen-Nutrient Stress and Identification of a Cell Death-Related Receptor-Like Kinase in Arabidopsis JO - Frontiers in Plant Science UR - https://www.frontiersin.org/articles/10.3389/fpls.2020.00377 VL - 11 SN - 1664-462X N2 - Nutrient availability, in particular the availability of sugar [carbon (C)] and nitrogen (N), is important for the regulation of plant metabolism and development. In addition to independent utilization of C and N nutrients, plants sense and respond to the balance of C and N nutrients (C/N-nutrient) available to them. High C/low N-nutrient stress has been shown to arrest early post-germinative growth while promoting progression to senescence in Arabidopsis. Although several signaling components of the C/N-nutrient response have been identified, the inclusive molecular basis of plant C/N-nutrient response remains unclear. This proteome analysis evaluated phosphorylation dynamics in response to high C/low N-nutrient stress. Phosphoproteomics under conditions of C/N-nutrient stress showed a global change in the phosphorylation status of proteins, including plasma membrane H+-ATPase, carbon and nitrogen metabolic enzymes and signaling proteins such as protein kinases and transcription factors. Further analyses suggested that SNF1-related protein kinase 1 (SnRK1) is involved in primary C/N-nutrient signal mediation via the transcriptional regulation of C/N-regulatory kinases. We also identified a leucine-rich repeat receptor-like kinase with extracellular malectin-like domain, named as LMK1, which was shown to possess cell death induction activity in plant leaves. These results provide important insight into the C/N-nutrient signaling pathways connecting nutrition stress to various cellular and physiological processes in plants. ER -