AUTHOR=Dell’Aquila Gianluca , Zauner Stefan , Heimerl Thomas , Kahnt Jörg , Samel-Gondesen Vera , Runge Simon , Hempel Franziska , Maier Uwe G. TITLE=Mobilization and Cellular Distribution of Phosphate in the Diatom Phaeodactylum tricornutum JOURNAL=Frontiers in Plant Science VOLUME=11 YEAR=2020 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00579 DOI=10.3389/fpls.2020.00579 ISSN=1664-462X ABSTRACT=

Unicellular organisms that live in marine environments must cope with considerable fluctuations in the availability of inorganic phosphate (Pi). Here, we investigated the extracellular Pi concentration-dependent expression, as well as the intracellular or extracellular localization, of phosphatases and phosphate transporters of the diatom Phaeodactylum tricornutum. We identified Pi-regulated plasma membrane-localized, ER-localized, and secreted phosphatases, in addition to plasma membrane-localized, vacuolar membrane-localized, and plastid-surrounding membrane-localized phosphate transporters that were also regulated in a Pi concentration-dependent manner. These studies not only add further knowledge to already existing transcriptomic data, but also highlight the capacity of the diatom to distribute Pi intracellularly and to mobilize Pi from extracellular and intracellular resources.